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The map label for this gene is uvrB

Identifier: 189025350

GI number: 189025350

Start: 132860

End: 134866

Strand: Direct

Name: uvrB

Synonym: TPASS_0116

Alternate gene names: 189025350

Gene position: 132860-134866 (Clockwise)

Preceding gene: 189025348

Following gene: 189025355

Centisome position: 11.66

GC content: 45.34

Gene sequence:

>2007_bases
ATGAAAGAATTTAAATTACACGCTTCTTTCCAACCTGCAGGCGATCAGATTGCCGCAATCGACGCACTCGTGCGTGGCTT
GCATGCGGGGGCGCGTTTTCAAACGCTCAAGGGTGTTACCGGTTCGGGGAAAACGTTTACAGTTGCAAACGTTATTGCAC
GCGTGCAAAAACCGACGCTTGTCATCAGTCATAATAAAACGCTCTCTGCACAATTGTACCGGGAATTTAAAGGTTTTTTC
CCAGACAATGCGGTGGAGTATTTTGTGTCGTACTACGATTATTATCAGCCTGAATCGTATGTCCCTGCTCGTGATCTCTA
CATTGAGAAAGATGCTTCTATCAATGCAGAGATAAATAGAATGCGTTTATCTGCAACGTTTAGTCTCATGGAACGTCGTG
ATGTTATTGTAGTCGCAACTGTTTCGTGTATTTACGGTCTTGGGTTGCCTGAATCCTGGCGTGATCTGCGTATCCACGTG
GAAGTCAACCAGTGTCTTGATCTTGAAGATCTCAAGCGGCAACTTGTATCTCTGCAATACGAGCGTAACGACGCGGTGCT
CGAGTGCGGTCGCTTCCGAGTTCGGGGAGACGTTATTGAAATTTTCCCTGCGTATTTAGAAGAATTTTATCGTATCGAGT
GCGATTGGGATCGAGTTGTTCGTATCCGTCGCATTCATCCTGTAAGCGGTGCTGTTCTAAGGGAATTTGAAGAACTTACC
GTTTACCCTGCAAAGCATTTTGTACTTAAAGAGGATGCAATCCCTCGCGCCATGGACCGTATTAGACAAGAACTCGATGA
ACGGTTGGTACAATTAACTCAAGAGAATAAGCTTGCAGAAGCGGCACGTTTAAAAACGCGCACAGAATACGATCTTGAAA
TGCTCGGAGAGATGGGGTATTGCCACGGTATAGAAAATTATTCTGCGCCGATTGCAGGTAGAAAATCTGGTGAACCGCCG
GTGACGCTTCTTCATTATTTTCCAAAGGATTTTGTGCTCTTTGTTGATGAAAGTCACGTAACACTTCCGCAGCTTGGTGC
AATGTATGAAGGAGATCGGGTGCGTAAACAGAATCTCATCGATTTTGGCTTTCGCTTGCCGTGTGCACGAGATAACCGTC
CACTGAAAGATTCGGAATTTGAGGCATTGTTAAATCAAGCAGTTTTTATTTCTGCAACTCCTGGGGTGAAGGAACGCACA
CAGTCTGTGCAAATAGTTGAACAACTGATTCGTCCCACTGGACTTTTAGATCCATGCATCGAAGTGAGAAAAACGGATGG
ACAAATAGAAGATATATGTCAGCGGGTAAAAGCTTGTAGTGCGCGGAACGAACGTAGTTTGGTGCTAACATTGACAAAAA
AAATGGCTGAAGATTTAACAGATTATTTCAATGGACTGGGAATAAGGACAAAGTATGTTCATTCGGAAATTGAAACTATT
GAACGCGTAGAAATTCTCACGAGTCTGCGTGCGGGGGAATGTGAGGTATTAGTTGGAATAAATTTATTACGAGAAGGTAT
TGATTTGCCCGAGGTGGCATTCATTGCTATTTTGGATGCCAATATAGTCGGTTTTTTACGTTCGACGACGAGTCTCATCC
AAATTATTGGTCGAGCAGCACGTAATGCTCGCGGAACTGTGGTAATGTACGCGGATGCAATCAGCGATGCGATGCGTGAA
GCAATAGAGGAAACTGCTCGACGGAGAAAGATTCAGATGGCGTATAATCGTGCTCATGGGATTACACCCCGTACGATTAA
AAAGTCTATTGAAGATATTCTGGTCCGTGAGCAGGAAGTAAAAAAAGACGCTGCGCGTGTACAGGTCGCACCCCTGTTGC
GCGCAGCAGACGCAGACGTGCGCACACATGCTGCACGCAAAAAAATGGTACAGGCGTTGCGCCTACACATGAAGGTTTGT
GCGCGTGAGTTACGATTTGAAGAGGCAGCCCTCATCCGAGACAAAATTTTGCAACTGCAAAGGCAAGACGAGCAAAACGG
GGTTTGA

Upstream 100 bases:

>100_bases
TGTCAACCAGAGGTTCTAAGAGAGCAAAATCAAAGGGAAAGGCACAGCATGTACTACAGATTTTTTAAATCCTATGGTAA
TAACCGATACTGCGAAGCAC

Downstream 100 bases:

>100_bases
TAGGGGAGGTGGAATCGAACAGCACGCGTTTTTACCATGTCACTTTCATTCCACAGACAAGGGTGCCGAAGTGGCGGTCG
GACCCGATGCTCTGAGTAAT

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MKEFKLHASFQPAGDQIAAIDALVRGLHAGARFQTLKGVTGSGKTFTVANVIARVQKPTLVISHNKTLSAQLYREFKGFF
PDNAVEYFVSYYDYYQPESYVPARDLYIEKDASINAEINRMRLSATFSLMERRDVIVVATVSCIYGLGLPESWRDLRIHV
EVNQCLDLEDLKRQLVSLQYERNDAVLECGRFRVRGDVIEIFPAYLEEFYRIECDWDRVVRIRRIHPVSGAVLREFEELT
VYPAKHFVLKEDAIPRAMDRIRQELDERLVQLTQENKLAEAARLKTRTEYDLEMLGEMGYCHGIENYSAPIAGRKSGEPP
VTLLHYFPKDFVLFVDESHVTLPQLGAMYEGDRVRKQNLIDFGFRLPCARDNRPLKDSEFEALLNQAVFISATPGVKERT
QSVQIVEQLIRPTGLLDPCIEVRKTDGQIEDICQRVKACSARNERSLVLTLTKKMAEDLTDYFNGLGIRTKYVHSEIETI
ERVEILTSLRAGECEVLVGINLLREGIDLPEVAFIAILDANIVGFLRSTTSLIQIIGRAARNARGTVVMYADAISDAMRE
AIEETARRRKIQMAYNRAHGITPRTIKKSIEDILVREQEVKKDAARVQVAPLLRAADADVRTHAARKKMVQALRLHMKVC
ARELRFEEAALIRDKILQLQRQDEQNGV

Sequences:

>Translated_668_residues
MKEFKLHASFQPAGDQIAAIDALVRGLHAGARFQTLKGVTGSGKTFTVANVIARVQKPTLVISHNKTLSAQLYREFKGFF
PDNAVEYFVSYYDYYQPESYVPARDLYIEKDASINAEINRMRLSATFSLMERRDVIVVATVSCIYGLGLPESWRDLRIHV
EVNQCLDLEDLKRQLVSLQYERNDAVLECGRFRVRGDVIEIFPAYLEEFYRIECDWDRVVRIRRIHPVSGAVLREFEELT
VYPAKHFVLKEDAIPRAMDRIRQELDERLVQLTQENKLAEAARLKTRTEYDLEMLGEMGYCHGIENYSAPIAGRKSGEPP
VTLLHYFPKDFVLFVDESHVTLPQLGAMYEGDRVRKQNLIDFGFRLPCARDNRPLKDSEFEALLNQAVFISATPGVKERT
QSVQIVEQLIRPTGLLDPCIEVRKTDGQIEDICQRVKACSARNERSLVLTLTKKMAEDLTDYFNGLGIRTKYVHSEIETI
ERVEILTSLRAGECEVLVGINLLREGIDLPEVAFIAILDANIVGFLRSTTSLIQIIGRAARNARGTVVMYADAISDAMRE
AIEETARRRKIQMAYNRAHGITPRTIKKSIEDILVREQEVKKDAARVQVAPLLRAADADVRTHAARKKMVQALRLHMKVC
ARELRFEEAALIRDKILQLQRQDEQNGV
>Mature_668_residues
MKEFKLHASFQPAGDQIAAIDALVRGLHAGARFQTLKGVTGSGKTFTVANVIARVQKPTLVISHNKTLSAQLYREFKGFF
PDNAVEYFVSYYDYYQPESYVPARDLYIEKDASINAEINRMRLSATFSLMERRDVIVVATVSCIYGLGLPESWRDLRIHV
EVNQCLDLEDLKRQLVSLQYERNDAVLECGRFRVRGDVIEIFPAYLEEFYRIECDWDRVVRIRRIHPVSGAVLREFEELT
VYPAKHFVLKEDAIPRAMDRIRQELDERLVQLTQENKLAEAARLKTRTEYDLEMLGEMGYCHGIENYSAPIAGRKSGEPP
VTLLHYFPKDFVLFVDESHVTLPQLGAMYEGDRVRKQNLIDFGFRLPCARDNRPLKDSEFEALLNQAVFISATPGVKERT
QSVQIVEQLIRPTGLLDPCIEVRKTDGQIEDICQRVKACSARNERSLVLTLTKKMAEDLTDYFNGLGIRTKYVHSEIETI
ERVEILTSLRAGECEVLVGINLLREGIDLPEVAFIAILDANIVGFLRSTTSLIQIIGRAARNARGTVVMYADAISDAMRE
AIEETARRRKIQMAYNRAHGITPRTIKKSIEDILVREQEVKKDAARVQVAPLLRAADADVRTHAARKKMVQALRLHMKVC
ARELRFEEAALIRDKILQLQRQDEQNGV

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Escherichia coli, GI1786996, Length=665, Percent_Identity=52.4812030075188, Blast_Score=672, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UVRB_TREPA (O83154)

Other databases:

- EMBL:   AE000520
- PIR:   C71365
- RefSeq:   NP_218556.1
- ProteinModelPortal:   O83154
- SMR:   O83154
- GeneID:   2610902
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0116
- NMPDR:   fig|243276.1.peg.115
- TIGR:   TP_0116
- HOGENOM:   HBG703949
- OMA:   AAQLCNE
- ProtClustDB:   PRK05298
- BioCyc:   TPAL243276:TP_0116-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00204
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00631

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR; SSF46600 UvrB_C

EC number: NA

Molecular weight: Translated: 76191; Mature: 76191

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFKLHASFQPAGDQIAAIDALVRGLHAGARFQTLKGVTGSGKTFTVANVIARVQKPTL
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEHHHHHHHHHCCCEE
VISHNKTLSAQLYREFKGFFPDNAVEYFVSYYDYYQPESYVPARDLYIEKDASINAEINR
EEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCCHHHH
MRLSATFSLMERRDVIVVATVSCIYGLGLPESWRDLRIHVEVNQCLDLEDLKRQLVSLQY
EEHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHH
ERNDAVLECGRFRVRGDVIEIFPAYLEEFYRIECDWDRVVRIRRIHPVSGAVLREFEELT
CCCCHHHHCCCEEECCHHHHHHHHHHHHHHEECCCHHHHHHHHEECCCCHHHHHHHHHHE
VYPAKHFVLKEDAIPRAMDRIRQELDERLVQLTQENKLAEAARLKTRTEYDLEMLGEMGY
ECCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHH
CHGIENYSAPIAGRKSGEPPVTLLHYFPKDFVLFVDESHVTLPQLGAMYEGDRVRKQNLI
HCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHCCCCCCCHHHHHHHH
DFGFRLPCARDNRPLKDSEFEALLNQAVFISATPGVKERTQSVQIVEQLIRPTGLLDPCI
HHCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHH
EVRKTDGQIEDICQRVKACSARNERSLVLTLTKKMAEDLTDYFNGLGIRTKYVHSEIETI
HHHCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ERVEILTSLRAGECEVLVGINLLREGIDLPEVAFIAILDANIVGFLRSTTSLIQIIGRAA
HHHHHHHHCCCCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNARGTVVMYADAISDAMREAIEETARRRKIQMAYNRAHGITPRTIKKSIEDILVREQEV
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KKDAARVQVAPLLRAADADVRTHAARKKMVQALRLHMKVCARELRFEEAALIRDKILQLQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQDEQNGV
HHHHCCCC
>Mature Secondary Structure
MKEFKLHASFQPAGDQIAAIDALVRGLHAGARFQTLKGVTGSGKTFTVANVIARVQKPTL
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEHHHHHHHHHCCCEE
VISHNKTLSAQLYREFKGFFPDNAVEYFVSYYDYYQPESYVPARDLYIEKDASINAEINR
EEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCCCCHHHH
MRLSATFSLMERRDVIVVATVSCIYGLGLPESWRDLRIHVEVNQCLDLEDLKRQLVSLQY
EEHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHH
ERNDAVLECGRFRVRGDVIEIFPAYLEEFYRIECDWDRVVRIRRIHPVSGAVLREFEELT
CCCCHHHHCCCEEECCHHHHHHHHHHHHHHEECCCHHHHHHHHEECCCCHHHHHHHHHHE
VYPAKHFVLKEDAIPRAMDRIRQELDERLVQLTQENKLAEAARLKTRTEYDLEMLGEMGY
ECCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHH
CHGIENYSAPIAGRKSGEPPVTLLHYFPKDFVLFVDESHVTLPQLGAMYEGDRVRKQNLI
HCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHCCCCCCCHHHHHHHH
DFGFRLPCARDNRPLKDSEFEALLNQAVFISATPGVKERTQSVQIVEQLIRPTGLLDPCI
HHCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHH
EVRKTDGQIEDICQRVKACSARNERSLVLTLTKKMAEDLTDYFNGLGIRTKYVHSEIETI
HHHCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ERVEILTSLRAGECEVLVGINLLREGIDLPEVAFIAILDANIVGFLRSTTSLIQIIGRAA
HHHHHHHHCCCCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNARGTVVMYADAISDAMREAIEETARRRKIQMAYNRAHGITPRTIKKSIEDILVREQEV
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KKDAARVQVAPLLRAADADVRTHAARKKMVQALRLHMKVCARELRFEEAALIRDKILQLQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQDEQNGV
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876