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The map label for this gene is thiD [H]

Identifier: 189025349

GI number: 189025349

Start: 131900

End: 132709

Strand: Reverse

Name: thiD [H]

Synonym: TPASS_0115

Alternate gene names: 189025349

Gene position: 132709-131900 (Counterclockwise)

Preceding gene: 189025351

Following gene: 189025346

Centisome position: 11.65

GC content: 54.32

Gene sequence:

>810_bases
ATGGTCAAGTTGCTTAGCATTGGTGGATCGGATGCTTCAGGTGGTGCGGGCATCGAGGCGGATCTGAAAACTTTCCAAGA
GTATGGAGCGTTCGGTGTGGCTACGCTCACCGCCATCGTTACTATGGACCCATCCCGGAACTGGTCGCATCGTGTACATT
CACTTGAGGAAGACTGTGTGCGCGATCAGCTTGAAACCGCATTTGCAGGCGTGGGGGTCAGCGCGGTGAAAAGCGGTATG
CTTGCCTCTGTCCATGCAATCGAATGTGTCGCGGAGTATCTCGAACGTTTTGCAGTTGCTGCATACGTCTTTGATCCTGT
CATGGTATGCAAAGGATCGGGAGATGCATTGCACCGTGAGTTGAACGAATTGATGATCCAGAAACTTTTGCCACGCGCGA
CAGTTGTTACTCCCAATCTTTTTGAAACCGCCCAGATTGCCGATATCAGCGTACCACGGACAGTGGACGAAATGAAGGAG
GGTGCACGTTTGATTCACGAGCGCGGCGCGTCGCACGTGTTCGTCAAAGGCGGCGGAAGACTCCCCGGTTGCAAGCACGC
TCTGGATGTTTTCTACGACGGCAAGACGTTTCACCTCGTTGAAGATGAACTTGTGCAGAGTGGATGGAATCACGGCGCGG
GCTGCACCGTATCTGCGGCTATTACTGCAGGACTGGGCCGAGGACTCACCGCCTACGACGCGATACTGAGTGCTAAGAGA
TTCGTGACTACAGGCCTCCGCCACGGATTCCAAGTCAACCAGTGGGTTGGAACAGGAAACCTCAGCAAATGGCGCGACCG
CTTCCACTGA

Upstream 100 bases:

>100_bases
ATGCTGTGCCTTTCCCTTTGATTTTGCTCTCTTAGAACCTCTGGTTGACAAACCACACCCATGGTGAATAGAGTGGACCC
CGCTTAAGAGGAGGCACGTT

Downstream 100 bases:

>100_bases
CTCAGGCGGTACATACGTGGGCGATCAGTGCTGGTATAGGTGCTTGAAGTATTCCAAGTCGGTTGAGAGGATCTTCTCCG
TGTCGGGGAGCGATTTCTTG

Product: phosphomethypyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MVKLLSIGGSDASGGAGIEADLKTFQEYGAFGVATLTAIVTMDPSRNWSHRVHSLEEDCVRDQLETAFAGVGVSAVKSGM
LASVHAIECVAEYLERFAVAAYVFDPVMVCKGSGDALHRELNELMIQKLLPRATVVTPNLFETAQIADISVPRTVDEMKE
GARLIHERGASHVFVKGGGRLPGCKHALDVFYDGKTFHLVEDELVQSGWNHGAGCTVSAAITAGLGRGLTAYDAILSAKR
FVTTGLRHGFQVNQWVGTGNLSKWRDRFH

Sequences:

>Translated_269_residues
MVKLLSIGGSDASGGAGIEADLKTFQEYGAFGVATLTAIVTMDPSRNWSHRVHSLEEDCVRDQLETAFAGVGVSAVKSGM
LASVHAIECVAEYLERFAVAAYVFDPVMVCKGSGDALHRELNELMIQKLLPRATVVTPNLFETAQIADISVPRTVDEMKE
GARLIHERGASHVFVKGGGRLPGCKHALDVFYDGKTFHLVEDELVQSGWNHGAGCTVSAAITAGLGRGLTAYDAILSAKR
FVTTGLRHGFQVNQWVGTGNLSKWRDRFH
>Mature_269_residues
MVKLLSIGGSDASGGAGIEADLKTFQEYGAFGVATLTAIVTMDPSRNWSHRVHSLEEDCVRDQLETAFAGVGVSAVKSGM
LASVHAIECVAEYLERFAVAAYVFDPVMVCKGSGDALHRELNELMIQKLLPRATVVTPNLFETAQIADISVPRTVDEMKE
GARLIHERGASHVFVKGGGRLPGCKHALDVFYDGKTFHLVEDELVQSGWNHGAGCTVSAAITAGLGRGLTAYDAILSAKR
FVTTGLRHGFQVNQWVGTGNLSKWRDRFH

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=245, Percent_Identity=34.2857142857143, Blast_Score=134, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6325378, Length=262, Percent_Identity=28.2442748091603, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324997, Length=261, Percent_Identity=27.5862068965517, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324517, Length=263, Percent_Identity=27.3764258555133, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 29001; Mature: 29001

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLLSIGGSDASGGAGIEADLKTFQEYGAFGVATLTAIVTMDPSRNWSHRVHSLEEDCV
CEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHH
RDQLETAFAGVGVSAVKSGMLASVHAIECVAEYLERFAVAAYVFDPVMVCKGSGDALHRE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHH
LNELMIQKLLPRATVVTPNLFETAQIADISVPRTVDEMKEGARLIHERGASHVFVKGGGR
HHHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
LPGCKHALDVFYDGKTFHLVEDELVQSGWNHGAGCTVSAAITAGLGRGLTAYDAILSAKR
CCCHHHHHHHEECCCEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
FVTTGLRHGFQVNQWVGTGNLSKWRDRFH
HHHHHHHCCCEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MVKLLSIGGSDASGGAGIEADLKTFQEYGAFGVATLTAIVTMDPSRNWSHRVHSLEEDCV
CEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHH
RDQLETAFAGVGVSAVKSGMLASVHAIECVAEYLERFAVAAYVFDPVMVCKGSGDALHRE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHH
LNELMIQKLLPRATVVTPNLFETAQIADISVPRTVDEMKEGARLIHERGASHVFVKGGGR
HHHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
LPGCKHALDVFYDGKTFHLVEDELVQSGWNHGAGCTVSAAITAGLGRGLTAYDAILSAKR
CCCHHHHHHHEECCCEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
FVTTGLRHGFQVNQWVGTGNLSKWRDRFH
HHHHHHHCCCEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]