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The map label for this gene is rpoN

Identifier: 189025345

GI number: 189025345

Start: 126971

End: 128398

Strand: Direct

Name: rpoN

Synonym: TPASS_0111

Alternate gene names: 189025345

Gene position: 126971-128398 (Clockwise)

Preceding gene: 189025344

Following gene: 189025347

Centisome position: 11.14

GC content: 47.9

Gene sequence:

>1428_bases
ATGCAGCAGCTTGTTCAACAGCAGCGGTTAGTTCTCAATCAGCGGTTGGTGCACGGGCTTTGTTTACTGCAGCTTTCTCG
GGGGGATTTGAAGACCGAGGTTCTCCGTGCCGTGCAACGCAATCCCCTTTTAGAAATACGTCCTTCGTCCGCTCGTCGCA
CTGGGAAGGCTTGTTACCTGTCGCTCGGTGATAGACGTGAGCGCATGCGCGCACGTGATCGCTTTCAGCAATTGCTGGAA
AATCAGCCCGATAAGCAGGTTGACAATATCCGAGCTGTATTGCGTGAACAAGTTTTTTATCAAAAACACGAAGCGATTGT
GCTCGATTTGGCGTGCGCGTTTATCCAGATGTTAGATGATCACGGTTTTTTTTCCATATCTCCTGCGATCTTTCAGAACA
TGTGTGGTAGTATGCCCACAGCCCTTCAAGAAAAAATACCGCAGGCAATAGCGCTCATTCAAAGACTTGAGCCACAAGGG
TGTGCTGTTTTTAATTTTAAAGAATCATTGGCAGTCCAAGCGCGGCTGCGCTTTGAGCGTTTCTCTGATCCCTTGTATCG
ATGTGTTATCAATCTACTAACACACCACAGCGAATTGCTTTTCTGCTCTGACAATATGTGTGATGGACGCGTATCCGTGC
ACGCGCTGACGACACAGATTAATAGTATGGGGCTTTGTGTGCAGAAGGTTTCGTCAAATGACGTGAAAGATATTTTGCTC
CTCATTAAGGAACTACATCCCTTTCCAGGTAAGTGTGTGTCGAACGCTCAGCGGGCTGACACGAACATGTTGCTTGAACC
AGATGTATTGATTACAAAAACTGCGCACGGGTTTGTTACACAGATTAACTGCACGGGCATCCCAACTGTGGTTTTCCGAA
ACGATTACTGCATGCATTCAAAAGCGGCAGAAAAAAACCACGCGTTGAAAGCATGCATGCACGACGCGCTCAGTCTTGTA
AGTATGCTCTCATACCGGGAACGTACCTTGCTGGATATCGCGAAAACAATAGTTCACTACCAATGCGGTTTCTTTGATCA
CGGACCTGCGAAGCTCACGCCCCTGAGAATGACAGATGTTGCACACAGGACCGGATTGAGCGTTTCAACAGTGTCCCGCA
TAGTGCGCGACAAATGGCTCCAGTACGGATCGCAACACTTTTCCTTAAGGTACTTTTTCTCCCCTCGGGTTTTATCCACC
GAAGAATATCGTGATCGGTCCAGTCTTGGACAGAATTACCCGAGTTCTCCGCACTCAAAAGTATCAGTTAAGTACCGGAT
ATCACGTCTCATACAAGAGGTACGAACGCAGCGTATATCCCTTTCCGACCGAAGGATAGCCCAGTTGCTCGGTGAGCAGG
GCATCAAGTGCGCAAGACGTACCGTAAATAAGTACCGATCAGAATTGCGCACTTGTTCTTCTTCTTAA

Upstream 100 bases:

>100_bases
CGCTAGTAGAAATGGAGAACCTAGAAACTACGCACCTGTGAGCGTTTCGCTCCTTTTCTGTATTCCTTTCGTGGGATTGG
TTTGCAGGGCACGTGGTGAT

Downstream 100 bases:

>100_bases
TTTTTAAGATACAGAGGGGTGTCCGCCAGCGCTCCCATCGGATCCCAAGGTTCGAGCGCTATTATTGGCTTCTCTAGCGC
ATCGAGCCACACCTGTGGTA

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MQQLVQQQRLVLNQRLVHGLCLLQLSRGDLKTEVLRAVQRNPLLEIRPSSARRTGKACYLSLGDRRERMRARDRFQQLLE
NQPDKQVDNIRAVLREQVFYQKHEAIVLDLACAFIQMLDDHGFFSISPAIFQNMCGSMPTALQEKIPQAIALIQRLEPQG
CAVFNFKESLAVQARLRFERFSDPLYRCVINLLTHHSELLFCSDNMCDGRVSVHALTTQINSMGLCVQKVSSNDVKDILL
LIKELHPFPGKCVSNAQRADTNMLLEPDVLITKTAHGFVTQINCTGIPTVVFRNDYCMHSKAAEKNHALKACMHDALSLV
SMLSYRERTLLDIAKTIVHYQCGFFDHGPAKLTPLRMTDVAHRTGLSVSTVSRIVRDKWLQYGSQHFSLRYFFSPRVLST
EEYRDRSSLGQNYPSSPHSKVSVKYRISRLIQEVRTQRISLSDRRIAQLLGEQGIKCARRTVNKYRSELRTCSSS

Sequences:

>Translated_475_residues
MQQLVQQQRLVLNQRLVHGLCLLQLSRGDLKTEVLRAVQRNPLLEIRPSSARRTGKACYLSLGDRRERMRARDRFQQLLE
NQPDKQVDNIRAVLREQVFYQKHEAIVLDLACAFIQMLDDHGFFSISPAIFQNMCGSMPTALQEKIPQAIALIQRLEPQG
CAVFNFKESLAVQARLRFERFSDPLYRCVINLLTHHSELLFCSDNMCDGRVSVHALTTQINSMGLCVQKVSSNDVKDILL
LIKELHPFPGKCVSNAQRADTNMLLEPDVLITKTAHGFVTQINCTGIPTVVFRNDYCMHSKAAEKNHALKACMHDALSLV
SMLSYRERTLLDIAKTIVHYQCGFFDHGPAKLTPLRMTDVAHRTGLSVSTVSRIVRDKWLQYGSQHFSLRYFFSPRVLST
EEYRDRSSLGQNYPSSPHSKVSVKYRISRLIQEVRTQRISLSDRRIAQLLGEQGIKCARRTVNKYRSELRTCSSS
>Mature_475_residues
MQQLVQQQRLVLNQRLVHGLCLLQLSRGDLKTEVLRAVQRNPLLEIRPSSARRTGKACYLSLGDRRERMRARDRFQQLLE
NQPDKQVDNIRAVLREQVFYQKHEAIVLDLACAFIQMLDDHGFFSISPAIFQNMCGSMPTALQEKIPQAIALIQRLEPQG
CAVFNFKESLAVQARLRFERFSDPLYRCVINLLTHHSELLFCSDNMCDGRVSVHALTTQINSMGLCVQKVSSNDVKDILL
LIKELHPFPGKCVSNAQRADTNMLLEPDVLITKTAHGFVTQINCTGIPTVVFRNDYCMHSKAAEKNHALKACMHDALSLV
SMLSYRERTLLDIAKTIVHYQCGFFDHGPAKLTPLRMTDVAHRTGLSVSTVSRIVRDKWLQYGSQHFSLRYFFSPRVLST
EEYRDRSSLGQNYPSSPHSKVSVKYRISRLIQEVRTQRISLSDRRIAQLLGEQGIKCARRTVNKYRSELRTCSSS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family

Homologues:

Organism=Escherichia coli, GI1789594, Length=510, Percent_Identity=24.5098039215686, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): RP54_TREPA (O83149)

Other databases:

- EMBL:   AE000520
- PIR:   F71364
- RefSeq:   NP_218551.1
- ProteinModelPortal:   O83149
- SMR:   O83149
- IntAct:   O83149
- GeneID:   2610882
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0111
- NMPDR:   fig|243276.1.peg.110
- TIGR:   TP_0111
- HOGENOM:   HBG250902
- OMA:   AVEHRNQ
- ProtClustDB:   CLSK2300133
- BioCyc:   TPAL243276:TP_0111-MONOMER
- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634
- TIGRFAMs:   TIGR02395

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD

EC number: NA

Molecular weight: Translated: 54281; Mature: 54281

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00717 SIGMA54_1; PS00718 SIGMA54_2; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLVQQQRLVLNQRLVHGLCLLQLSRGDLKTEVLRAVQRNPLLEIRPSSARRTGKACYL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEE
SLGDRRERMRARDRFQQLLENQPDKQVDNIRAVLREQVFYQKHEAIVLDLACAFIQMLDD
ECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HGFFSISPAIFQNMCGSMPTALQEKIPQAIALIQRLEPQGCAVFNFKESLAVQARLRFER
CCCEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
FSDPLYRCVINLLTHHSELLFCSDNMCDGRVSVHALTTQINSMGLCVQKVSSNDVKDILL
HCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LIKELHPFPGKCVSNAQRADTNMLLEPDVLITKTAHGFVTQINCTGIPTVVFRNDYCMHS
HHHHHCCCCCHHHCCCHHCCCCEEECCCEEEEECCCCEEEEEECCCCCEEEECCCCCCCC
KAAEKNHALKACMHDALSLVSMLSYRERTLLDIAKTIVHYQCGFFDHGPAKLTPLRMTDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AHRTGLSVSTVSRIVRDKWLQYGSQHFSLRYFFSPRVLSTEEYRDRSSLGQNYPSSPHSK
HHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEECHHHHHHHHHHCCCCCCCCCCH
VSVKYRISRLIQEVRTQRISLSDRRIAQLLGEQGIKCARRTVNKYRSELRTCSSS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQQLVQQQRLVLNQRLVHGLCLLQLSRGDLKTEVLRAVQRNPLLEIRPSSARRTGKACYL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEE
SLGDRRERMRARDRFQQLLENQPDKQVDNIRAVLREQVFYQKHEAIVLDLACAFIQMLDD
ECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HGFFSISPAIFQNMCGSMPTALQEKIPQAIALIQRLEPQGCAVFNFKESLAVQARLRFER
CCCEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
FSDPLYRCVINLLTHHSELLFCSDNMCDGRVSVHALTTQINSMGLCVQKVSSNDVKDILL
HCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LIKELHPFPGKCVSNAQRADTNMLLEPDVLITKTAHGFVTQINCTGIPTVVFRNDYCMHS
HHHHHCCCCCHHHCCCHHCCCCEEECCCEEEEECCCCEEEEEECCCCCEEEECCCCCCCC
KAAEKNHALKACMHDALSLVSMLSYRERTLLDIAKTIVHYQCGFFDHGPAKLTPLRMTDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
AHRTGLSVSTVSRIVRDKWLQYGSQHFSLRYFFSPRVLSTEEYRDRSSLGQNYPSSPHSK
HHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEECHHHHHHHHHHCCCCCCCCCCH
VSVKYRISRLIQEVRTQRISLSDRRIAQLLGEQGIKCARRTVNKYRSELRTCSSS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876