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The map label for this gene is ushA [C]

Identifier: 189025338

GI number: 189025338

Start: 113855

End: 115636

Strand: Direct

Name: ushA [C]

Synonym: TPASS_0104

Alternate gene names: 189025338

Gene position: 113855-115636 (Clockwise)

Preceding gene: 189025337

Following gene: 189025339

Centisome position: 9.99

GC content: 48.43

Gene sequence:

>1782_bases
ATGAAAAGGTTTATTCCCCATCGGGTGATTCACGCGGTGTGTATCGGGCTTGCACTTGTAGGTTGTAGGAAACTCGATTC
TCGTGCGGGGGATTTTGAGTTAACGATTATACATATCAACGATCATCATTCGCATTTGGAACCAGAACCCTTAGAGCTTG
CAGTGGCAGGGGAAAGACTCAGAGCGGCTGTAGGCGGTTATGCGGCGCTTGTGCACGAGATACAACGGTTGCGTGCGGAG
TCGAAGAACGCATTGGTACTGCATGCAGGAGATGCACTCATAGGTACGCTGTATTCTACCCTCTTTAGAGGGCGTGCGGA
CGCGGTGCTGATGAACCATGCAGGATTTGATTTTTTTACCCTTGGCAATCACGAATTTGATAATGGGAATGAGGGACTCA
AAGAATTTCTGCACTATTTGGAAGTGCCAGTTCTCTCTGCAAATGTGGTTCCTAATGCTGCCAGCACGTTGCATGGCTTG
TGGAAGCCGAGCGCTATTGTGGAGCGTGCAGGTGAGCGTATTGGGGTTATCGGACTTGATACGGTAAAGAAAACCGTGGA
GTCATCCAGTCCCGGTAAGGATATCAATTTTATTGATGAGATAGAGGCGGTGCGTCGTGCAACTGTTGAAATGCAGCAGC
AAGGAGTAAATAAAATAATCCTCCTTTCTCATGCAGGTTTTGAGAAGAACTGTGAAATTGCTCAGAACATTTCTGGTATT
GACGTCATCGTGTCAGGTGATACCCACTACCTTTTGGGGGATGAATCACTCGGACGGCTAGGTCTTCCGGTAGTTGGTGA
ATATCCCAGAAAGATTATGTCCCCTGCAGGGGAGCCTGTGTATGTGGTAGAGGCGTGGGAGTATGGTAAGTGTCTGGGCG
AGCTGAACGTAGTCTTTGACCGAACAGGAGTAATAACGAGTGCAGTAGGCATGCCGCGTTTTTTGTTACATACGAATACA
TTGCAAAAAAAAGGAGCGGATAGAAAAAATTATCCTCTTGAGGAGGCAGAGCGTGAAGCGCTGCTTGTGGCACTGAGGAT
GACGCCAGAGATTATATTTGCGCAGGAGAATGATCAGATTATATCTGTGTTGGAAGAATTTAAAAAGGAAAAGGAGGCGC
TTGGTGCGCAGGCAATTGGCGTAATTACCGGTGCCTCAATGCGAGGTGGTTCTGTGCATCGAGTTCCCGATGCACAGAAT
CCACAGGGTTCGGTTGCAACGCGGTTTGTAGCAGAGACGATGCTCTCAGACATTCAAAGTTTTGGTGCGGGGAAGGTAGA
TTGCGTAATTCAAAATGCAGGCGGTGCGCGGTCAAATATTCAGCCTGGTGAGATTACGTATAATGACGCATACACGCTCC
TCCCCTTTAGTAACACGCTGGTGTTGGTGGACGTCAGCGGTGCAGAGTTGAAACAAATTATAGAGGATGCATTGCAGTTT
GCACTTGGTGATGGTTCCACGGGAGCCTTCCCCTATGGGGCGGGTGTCCGGTATGAAGCGCGCCAAGAACCAGATGAACA
TGGCAAACGAGTGATAAAGCTTGAGGTGCAAAAAAAAGATGGAGCGTGGGTGCCAGTAGATGAGCGCGCGCCGTATCGGT
TGGGTGTGAACTCGTACATTGCGCGGGGAAAAGACGGATATAAAACGCTCGGAGAGATTGTCAGTACGCGCGGAGCTGAG
GATACGTATCTGCGTGATGCGGAGTCTTTGATTAAGTTTTTGCGTGCGCATAAAAATTTTCGTGCATACACAGATTCCAA
TGTGATATTCCGTCTTAAATAG

Upstream 100 bases:

>100_bases
TGAGCCGTCGTTCATGTGTCTCCGATACGGTGTGGTCTAGGTTCCGTACCGTGCGGGCACGGAACACATCGAGCGGACGC
GTCTGTTCGTGGAGGATATT

Downstream 100 bases:

>100_bases
TAGGAAGTAACTTACATTAGAGGCCTGTAAAGAACTACGTTCTTTACAGGCTGTGCCAATCTGCTTTTCCGGGAAAGACA
AAGGGTATGCCACGTTAGGA

Product: 5'-nucleotidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKRFIPHRVIHAVCIGLALVGCRKLDSRAGDFELTIIHINDHHSHLEPEPLELAVAGERLRAAVGGYAALVHEIQRLRAE
SKNALVLHAGDALIGTLYSTLFRGRADAVLMNHAGFDFFTLGNHEFDNGNEGLKEFLHYLEVPVLSANVVPNAASTLHGL
WKPSAIVERAGERIGVIGLDTVKKTVESSSPGKDINFIDEIEAVRRATVEMQQQGVNKIILLSHAGFEKNCEIAQNISGI
DVIVSGDTHYLLGDESLGRLGLPVVGEYPRKIMSPAGEPVYVVEAWEYGKCLGELNVVFDRTGVITSAVGMPRFLLHTNT
LQKKGADRKNYPLEEAEREALLVALRMTPEIIFAQENDQIISVLEEFKKEKEALGAQAIGVITGASMRGGSVHRVPDAQN
PQGSVATRFVAETMLSDIQSFGAGKVDCVIQNAGGARSNIQPGEITYNDAYTLLPFSNTLVLVDVSGAELKQIIEDALQF
ALGDGSTGAFPYGAGVRYEARQEPDEHGKRVIKLEVQKKDGAWVPVDERAPYRLGVNSYIARGKDGYKTLGEIVSTRGAE
DTYLRDAESLIKFLRAHKNFRAYTDSNVIFRLK

Sequences:

>Translated_593_residues
MKRFIPHRVIHAVCIGLALVGCRKLDSRAGDFELTIIHINDHHSHLEPEPLELAVAGERLRAAVGGYAALVHEIQRLRAE
SKNALVLHAGDALIGTLYSTLFRGRADAVLMNHAGFDFFTLGNHEFDNGNEGLKEFLHYLEVPVLSANVVPNAASTLHGL
WKPSAIVERAGERIGVIGLDTVKKTVESSSPGKDINFIDEIEAVRRATVEMQQQGVNKIILLSHAGFEKNCEIAQNISGI
DVIVSGDTHYLLGDESLGRLGLPVVGEYPRKIMSPAGEPVYVVEAWEYGKCLGELNVVFDRTGVITSAVGMPRFLLHTNT
LQKKGADRKNYPLEEAEREALLVALRMTPEIIFAQENDQIISVLEEFKKEKEALGAQAIGVITGASMRGGSVHRVPDAQN
PQGSVATRFVAETMLSDIQSFGAGKVDCVIQNAGGARSNIQPGEITYNDAYTLLPFSNTLVLVDVSGAELKQIIEDALQF
ALGDGSTGAFPYGAGVRYEARQEPDEHGKRVIKLEVQKKDGAWVPVDERAPYRLGVNSYIARGKDGYKTLGEIVSTRGAE
DTYLRDAESLIKFLRAHKNFRAYTDSNVIFRLK
>Mature_593_residues
MKRFIPHRVIHAVCIGLALVGCRKLDSRAGDFELTIIHINDHHSHLEPEPLELAVAGERLRAAVGGYAALVHEIQRLRAE
SKNALVLHAGDALIGTLYSTLFRGRADAVLMNHAGFDFFTLGNHEFDNGNEGLKEFLHYLEVPVLSANVVPNAASTLHGL
WKPSAIVERAGERIGVIGLDTVKKTVESSSPGKDINFIDEIEAVRRATVEMQQQGVNKIILLSHAGFEKNCEIAQNISGI
DVIVSGDTHYLLGDESLGRLGLPVVGEYPRKIMSPAGEPVYVVEAWEYGKCLGELNVVFDRTGVITSAVGMPRFLLHTNT
LQKKGADRKNYPLEEAEREALLVALRMTPEIIFAQENDQIISVLEEFKKEKEALGAQAIGVITGASMRGGSVHRVPDAQN
PQGSVATRFVAETMLSDIQSFGAGKVDCVIQNAGGARSNIQPGEITYNDAYTLLPFSNTLVLVDVSGAELKQIIEDALQF
ALGDGSTGAFPYGAGVRYEARQEPDEHGKRVIKLEVQKKDGAWVPVDERAPYRLGVNSYIARGKDGYKTLGEIVSTRGAE
DTYLRDAESLIKFLRAHKNFRAYTDSNVIFRLK

Specific function: Degradation Of External Udp-Glucose To Uridine Monophosphate And Glucose-1-Phosphate, Which Can Then Be Used By The Cell. [C]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family

Homologues:

Organism=Homo sapiens, GI4505467, Length=537, Percent_Identity=31.2849162011173, Blast_Score=186, Evalue=5e-47,
Organism=Escherichia coli, GI1786687, Length=562, Percent_Identity=27.5800711743772, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24641187, Length=532, Percent_Identity=29.1353383458647, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI19922446, Length=535, Percent_Identity=27.1028037383178, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24654424, Length=535, Percent_Identity=27.1028037383178, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI221329836, Length=559, Percent_Identity=25.7602862254025, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI19922444, Length=567, Percent_Identity=26.1022927689594, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI28573524, Length=572, Percent_Identity=25.6993006993007, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI19921980, Length=528, Percent_Identity=26.1363636363636, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652512, Length=528, Percent_Identity=26.1363636363636, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161076508, Length=528, Percent_Identity=26.1363636363636, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): 5NTD_TREPA (O83142)

Other databases:

- EMBL:   AE000520
- PIR:   E71365
- RefSeq:   NP_218544.1
- ProteinModelPortal:   O83142
- IntAct:   O83142
- GeneID:   2610818
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0104
- NMPDR:   fig|243276.1.peg.103
- TIGR:   TP_0104
- HOGENOM:   HBG534106
- OMA:   RIATRAQ
- ProtClustDB:   CLSK793390
- BioCyc:   TPAL243276:TP_0104-MONOMER
- BRENDA:   3.1.3.5
- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843
- InterPro:   IPR006420
- Gene3D:   G3DSA:3.90.780.10
- PANTHER:   PTHR11575
- PRINTS:   PR01607
- TIGRFAMs:   TIGR01530

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos; SSF55816 5'-Nucleotdase_C

EC number: =3.1.3.5

Molecular weight: Translated: 64872; Mature: 64872

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00785 5_NUCLEOTIDASE_1; PS00786 5_NUCLEOTIDASE_2; PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFIPHRVIHAVCIGLALVGCRKLDSRAGDFELTIIHINDHHSHLEPEPLELAVAGERL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCEEEEECHHHH
RAAVGGYAALVHEIQRLRAESKNALVLHAGDALIGTLYSTLFRGRADAVLMNHAGFDFFT
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCEEE
LGNHEFDNGNEGLKEFLHYLEVPVLSANVVPNAASTLHGLWKPSAIVERAGERIGVIGLD
CCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCHHHHHHCCCEEEEEEHH
TVKKTVESSSPGKDINFIDEIEAVRRATVEMQQQGVNKIILLSHAGFEKNCEIAQNISGI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCE
DVIVSGDTHYLLGDESLGRLGLPVVGEYPRKIMSPAGEPVYVVEAWEYGKCLGELNVVFD
EEEEECCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHCEEEEEE
RTGVITSAVGMPRFLLHTNTLQKKGADRKNYPLEEAEREALLVALRMTPEIIFAQENDQI
CCCHHHHHCCCCHHHHCCCHHHHCCCCCCCCCCCHHHHHHEEEEEECCCEEEEECCCCHH
ISVLEEFKKEKEALGAQAIGVITGASMRGGSVHRVPDAQNPQGSVATRFVAETMLSDIQS
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
FGAGKVDCVIQNAGGARSNIQPGEITYNDAYTLLPFSNTLVLVDVSGAELKQIIEDALQF
HCCCCEEEEEECCCCCCCCCCCCEEEECCCEEEEECCCEEEEEECCCHHHHHHHHHHHHH
ALGDGSTGAFPYGAGVRYEARQEPDEHGKRVIKLEVQKKDGAWVPVDERAPYRLGVNSYI
HCCCCCCCCCCCCCCCEECCCCCCHHHCCEEEEEEEEECCCCEEECCCCCCEEECCHHHH
ARGKDGYKTLGEIVSTRGAEDTYLRDAESLIKFLRAHKNFRAYTDSNVIFRLK
HCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEC
>Mature Secondary Structure
MKRFIPHRVIHAVCIGLALVGCRKLDSRAGDFELTIIHINDHHSHLEPEPLELAVAGERL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCEEEEECHHHH
RAAVGGYAALVHEIQRLRAESKNALVLHAGDALIGTLYSTLFRGRADAVLMNHAGFDFFT
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCEEE
LGNHEFDNGNEGLKEFLHYLEVPVLSANVVPNAASTLHGLWKPSAIVERAGERIGVIGLD
CCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCHHHHHHCCCEEEEEEHH
TVKKTVESSSPGKDINFIDEIEAVRRATVEMQQQGVNKIILLSHAGFEKNCEIAQNISGI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCE
DVIVSGDTHYLLGDESLGRLGLPVVGEYPRKIMSPAGEPVYVVEAWEYGKCLGELNVVFD
EEEEECCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHCEEEEEE
RTGVITSAVGMPRFLLHTNTLQKKGADRKNYPLEEAEREALLVALRMTPEIIFAQENDQI
CCCHHHHHCCCCHHHHCCCHHHHCCCCCCCCCCCHHHHHHEEEEEECCCEEEEECCCCHH
ISVLEEFKKEKEALGAQAIGVITGASMRGGSVHRVPDAQNPQGSVATRFVAETMLSDIQS
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
FGAGKVDCVIQNAGGARSNIQPGEITYNDAYTLLPFSNTLVLVDVSGAELKQIIEDALQF
HCCCCEEEEEECCCCCCCCCCCCEEEECCCEEEEECCCEEEEEECCCHHHHHHHHHHHHH
ALGDGSTGAFPYGAGVRYEARQEPDEHGKRVIKLEVQKKDGAWVPVDERAPYRLGVNSYI
HCCCCCCCCCCCCCCCEECCCCCCHHHCCEEEEEEEEECCCCEEECCCCCCEEECCHHHH
ARGKDGYKTLGEIVSTRGAEDTYLRDAESLIKFLRAHKNFRAYTDSNVIFRLK
HCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9665876