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The map label for this gene is rlmN

Identifier: 189025302

GI number: 189025302

Start: 74008

End: 75030

Strand: Direct

Name: rlmN

Synonym: TPASS_0068

Alternate gene names: 189025302

Gene position: 74008-75030 (Clockwise)

Preceding gene: 189025301

Following gene: 189025306

Centisome position: 6.5

GC content: 53.18

Gene sequence:

>1023_bases
ATGGAGTGGTGCTGCGCCCTTTCGGGGCTTTTGCCAGAAGAAATCCAGAAGGTGTGTGCGTTTGCTGAGCGCTTTCGTGG
GGTGCAGGTGTTCAGATGGATTGCCGCAGGGTGCACTGACTTCCATGCGATGAGTGATCTCTCTTCTGAGACGCGTGCAC
GCCTGGCGAGGGCGTGCGTCATCTCGGACACTCGTGTCTATACCACGCTGCGTGATGTGGATGGTACGCTCAAGCTGGGT
ATTGAACTGAAAGATAAACGGCGCGTAGAGGCAGTCTTACTCGTCGATCAAGTCTCGCGTAAGACTGCTTGTCTATCCTG
TCAAGTCGGCTGCCCTATGGCGTGCGCGTTTTGTCAAACAGGCCAGTTGGGTTTCGCGCGAAACCTTTCTGCCTCAGAGA
TCGTCGAGCAGTTCCTTCATCTGGAACGATGTGTCGGTACATTGGATAATGTTGTGTTTATGGGAATGGGTGAGCCCATG
CTCAATCTGGATGCGGTGTGTAGAGCTATTGAGATACTGTCTCATCCACAGGGTCGTGACCTATCTGAAAAACGTATTAC
TATTTCTACGTCTGGACATTGCCGTGGTATTTATTCGCTTGCTGACCGCGCACTGCAGGTTCGCTTGGCGGTGTCTTTAA
CCACCGCGAATGCACCGTTGCGCGCACGCCTCATGCCACGTGCTGCGCACGACAGTTTAGCAAAACTGAAAAGCGCTATT
CGCTATTTTAACGAGAAGAGTGGAAAGCGTGTGACACTCGAGCTCGCCCTCATGCGGGGAGTGAATACTTCTGAACGGCA
TGCGCAAGAAGTTATCGATTTTGCACATGGGCTTAACGTGCACGTGAACTTAATTCCCTGGAATCCGGTAGCATCAATCC
ACTTTGAAACACCTCGGGAAGTGGAGGTTGCGCATTTTGAGGCGCTTCTCATGCGCGCCCGCATCCCCGTGACACGCCGC
TATCAGCGTGGGAATGGCATTGGAGGCGCATGCGGACAACTAGGTAAAACAGCCGGCGTGTAA

Upstream 100 bases:

>100_bases
TGCGCGATGCCTGTCGATTCGGCGCTTTTTGTGTGGTGGTCGGGGTCCTCCTCTTCGTCGGAGGTGGTGCAGGACTTTGC
GACGAGGGGGTGGAGCGTAT

Downstream 100 bases:

>100_bases
CTCTTTCGCTCCGTTTGTAGATGTTTGTACGTGTGGCCATGCTCGTTCCTGTTTTTCAGGAAAGTTTCTTTGAGGCAGCC
AGCGCTTTTGCCTTGCTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MEWCCALSGLLPEEIQKVCAFAERFRGVQVFRWIAAGCTDFHAMSDLSSETRARLARACVISDTRVYTTLRDVDGTLKLG
IELKDKRRVEAVLLVDQVSRKTACLSCQVGCPMACAFCQTGQLGFARNLSASEIVEQFLHLERCVGTLDNVVFMGMGEPM
LNLDAVCRAIEILSHPQGRDLSEKRITISTSGHCRGIYSLADRALQVRLAVSLTTANAPLRARLMPRAAHDSLAKLKSAI
RYFNEKSGKRVTLELALMRGVNTSERHAQEVIDFAHGLNVHVNLIPWNPVASIHFETPREVEVAHFEALLMRARIPVTRR
YQRGNGIGGACGQLGKTAGV

Sequences:

>Translated_340_residues
MEWCCALSGLLPEEIQKVCAFAERFRGVQVFRWIAAGCTDFHAMSDLSSETRARLARACVISDTRVYTTLRDVDGTLKLG
IELKDKRRVEAVLLVDQVSRKTACLSCQVGCPMACAFCQTGQLGFARNLSASEIVEQFLHLERCVGTLDNVVFMGMGEPM
LNLDAVCRAIEILSHPQGRDLSEKRITISTSGHCRGIYSLADRALQVRLAVSLTTANAPLRARLMPRAAHDSLAKLKSAI
RYFNEKSGKRVTLELALMRGVNTSERHAQEVIDFAHGLNVHVNLIPWNPVASIHFETPREVEVAHFEALLMRARIPVTRR
YQRGNGIGGACGQLGKTAGV
>Mature_340_residues
MEWCCALSGLLPEEIQKVCAFAERFRGVQVFRWIAAGCTDFHAMSDLSSETRARLARACVISDTRVYTTLRDVDGTLKLG
IELKDKRRVEAVLLVDQVSRKTACLSCQVGCPMACAFCQTGQLGFARNLSASEIVEQFLHLERCVGTLDNVVFMGMGEPM
LNLDAVCRAIEILSHPQGRDLSEKRITISTSGHCRGIYSLADRALQVRLAVSLTTANAPLRARLMPRAAHDSLAKLKSAI
RYFNEKSGKRVTLELALMRGVNTSERHAQEVIDFAHGLNVHVNLIPWNPVASIHFETPREVEVAHFEALLMRARIPVTRR
YQRGNGIGGACGQLGKTAGV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=322, Percent_Identity=36.0248447204969, Blast_Score=191, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_TREPA (O83107)

Other databases:

- EMBL:   AE000520
- PIR:   D71371
- RefSeq:   NP_218508.1
- ProteinModelPortal:   O83107
- IntAct:   O83107
- GeneID:   2611395
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0068
- NMPDR:   fig|243276.1.peg.67
- TIGR:   TP_0068
- HOGENOM:   HBG335345
- OMA:   VQKYFDE
- ProtClustDB:   CLSK218510
- BioCyc:   TPAL243276:TP_0068-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 37520; Mature: 37520

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWCCALSGLLPEEIQKVCAFAERFRGVQVFRWIAAGCTDFHAMSDLSSETRARLARACV
CCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHH
ISDTRVYTTLRDVDGTLKLGIELKDKRRVEAVLLVDQVSRKTACLSCQVGCPMACAFCQT
CCCCHHEEHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCHHEEEEECCCCCHHHHHHCC
GQLGFARNLSASEIVEQFLHLERCVGTLDNVVFMGMGEPMLNLDAVCRAIEILSHPQGRD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
LSEKRITISTSGHCRGIYSLADRALQVRLAVSLTTANAPLRARLMPRAAHDSLAKLKSAI
CCCCEEEEECCCCCCHHHHHHHHHHEEEEEEEEEECCCCHHHHCCCCHHHHHHHHHHHHH
RYFNEKSGKRVTLELALMRGVNTSERHAQEVIDFAHGLNVHVNLIPWNPVASIHFETPRE
HHHHCCCCCEEEEEEEEHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCC
VEVAHFEALLMRARIPVTRRYQRGNGIGGACGQLGKTAGV
HHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MEWCCALSGLLPEEIQKVCAFAERFRGVQVFRWIAAGCTDFHAMSDLSSETRARLARACV
CCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHH
ISDTRVYTTLRDVDGTLKLGIELKDKRRVEAVLLVDQVSRKTACLSCQVGCPMACAFCQT
CCCCHHEEHHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCHHEEEEECCCCCHHHHHHCC
GQLGFARNLSASEIVEQFLHLERCVGTLDNVVFMGMGEPMLNLDAVCRAIEILSHPQGRD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
LSEKRITISTSGHCRGIYSLADRALQVRLAVSLTTANAPLRARLMPRAAHDSLAKLKSAI
CCCCEEEEECCCCCCHHHHHHHHHHEEEEEEEEEECCCCHHHHCCCCHHHHHHHHHHHHH
RYFNEKSGKRVTLELALMRGVNTSERHAQEVIDFAHGLNVHVNLIPWNPVASIHFETPRE
HHHHCCCCCEEEEEEEEHHCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCC
VEVAHFEALLMRARIPVTRRYQRGNGIGGACGQLGKTAGV
HHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876