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The map label for this gene is pcrA [H]

Identifier: 188587404

GI number: 188587404

Start: 3039434

End: 3041698

Strand: Reverse

Name: pcrA [H]

Synonym: Nther_2813

Alternate gene names: 188587404

Gene position: 3041698-3039434 (Counterclockwise)

Preceding gene: 188587405

Following gene: 188587403

Centisome position: 96.09

GC content: 36.87

Gene sequence:

>2265_bases
ATGATTGGAGACTTTTCCGGGTCAGGTAATCAAAACAAAGGCAATGGTAAAGATCCCCTTGATAAATTAATTGATTTTTC
AAAATACAAGCGCAGGAAACAAGGCCAAGATAAACATGAAGCCAGTAATACTGAGGAATCATTGAAAAACAAGGAAACAA
ACCATGCCAGTATTTTTCCTTCCCAATACCCTGAACCACCTGTTGACGATGAAATCAGAGCTAGATTCCAACATTTACAG
TCACTGATTGACAAAATAAATAATCAATTGGAACAAGAGGCTAGGCCTGGAGATTTTCCTAACAATATCAGTCAAAGTGC
CAGTGACTTCAAAATAAATTATCCTGAAGAGCTTAACCCACAACAGCTGGAGGCAGTTACTTACTGCAATGGGCCTTTAT
TAATCATAGCAGGCGCAGGGACTGGTAAAACTAGAACTTTGGTATACCGGGTTTCTTATTTACTAGAATCGGGGATTCCC
CCCCAAGAAATTTTACTTTTAACATTTACACGAAAGGCTGCCCGAGAAATGCTGGAAAGAGTCAAAAAACTCTTAGGCAC
TGAAAGTACTGATAGAATTAAAGGAGGGACTTTCCATTCTTTTGCAAATAATGTCTTGAGAAGATACTCGGGCCTAGTTG
GTTTATCCCCTGATTTTTCTGTTTTGGATCAGATTGATTCACAAGATGTGGTGGATTTACTTAGAACTGAATATGATTTT
CACAGACAAGAAAAAGCTTTTCCCAAGAAAGAGCGGATTTTCGAGATTATTTCCAAAAGCAGGAATACTCAAAAATCAAT
AGGTCAAGTGATAAGTGAAGAGTTTTCCGGATTAGATGATTATATAGATGAAATTGATACTATCGCCCAAGCTTACCATA
AATACAAAAGACACTATAATCTGATGGATTTTGATGACCTGTTATTGGTTTTACGAGATAATCTGAGAGACAATTCTACT
TTCCGTAAACAGTTGTCTTCGGAATTATCCTACATACTTGTAGACGAATTCCAAGATACTAATGTTGTTCAAAACGAGAT
AGTTAATTTGCTTGGTTCACAATCTAGGAATATCACCGTAGTAGGAGATGATTTCCAAAGCATCTATGCTTTTAGAGGAG
CTAATTGGGAAAATATCCTCTTGTTTCCTCAAAATTATCCCGATGCAAAGTTAATAAAGCTTGAGAAAAACTACAGATCG
CACAATAAAATTCTCAAATTTGCCAATGAAATAGTGGATTCGGCTCAAATAGGCTATCAAAAACATCTCTCCTCTGAGTT
CCATGAGGGTACGCTACCTCAATTTATTAAATATTATTCCCAGGAGGAGGAAGCAGAAGCTATAGTTTCTGAGATACTTG
CTTTACGGGAACAAGGCTTTTCATATAAGGATATGGCTGTATTGTACAGAGCTGGTTTCCACGGCAATTATATTCAGGCA
GAACTTTTGAGGAGGAAAATTCCATACGTTGTTTACGGTGGTATTAAGTTTATGGAGAGAAAGCATGTTAAAGATCTGGT
CTCTTATTTAAGGTTAACAGTCAATCCCATAGATGCTGTTTCTTGGAATCGATTTTTAAAAATGATTCCAGGGATCGGAA
GGGTAAGAGCTAGCCAAATTGTTGAAGAAATCACTGAACAGGATGGGCGTTTGAACTTTAATAATTTTAAGGGGACGAGC
TATTATAAGTCTTTATTATCTTTACAAGAAGTTTTATTAGAAGCTGGAAAAAAGGGGATTTCACCTTCTAACAGTGTTGC
TCGGATAATTGAATATTATTACCCAATACTGGAAAAACAAGAAGTGGATGCTTCGGAACGAAAAAATGACTTAGAAGTTC
TCTATCATTTATCCCAGAATTACTCGGAATTAGAACAATTTTTGGCTGATTTATCCTTAGAACCTCCTGCCAATAAATTT
CAACAAGAAACAACCCCTGCATTAAATGAAGCTGAAGACGACGATAGCTTGGTGATATCTACCATTCACTCTGCTAAGGG
TCTAGAATGGAATACGGTTTTTATTCCTCACTTACTAGATGGCTTGTTCCCTAGTTCTAAGTCCCTGAGAGATCCATTTA
TACTAGAAGAAGAACGTCGATTGTTTTATGTGGCTATAACACGGGCTAAAGAAAAACTATATCTATCAATGCCCCATCAG
GTAGTATCGTATAATGGTTACTTTATCCAACCCTCTAGATTTTTAAAAGATCTGGACCCTGCTTGCTATCATTTTCAGGC
CTTTTTCCAGGATAACAAAGAATAA

Upstream 100 bases:

>100_bases
TATGGCGGGACTTTAGAAGTCCCGCTTTTTTTATGTTTATTCGATTATGTTATAATTTGATAAGATGTTAAATAATTTGA
GAAGAACCGAGGTGACAACC

Downstream 100 bases:

>100_bases
GTAAAGTTATAAAAATAAATCTATTTTTCAGGGTTTTCACTTTATGAGAAAATTTGATAGAATATTAGTGACGCGAAATA
TTATATATTAATTCAAAAAT

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 754; Mature: 754

Protein sequence:

>754_residues
MIGDFSGSGNQNKGNGKDPLDKLIDFSKYKRRKQGQDKHEASNTEESLKNKETNHASIFPSQYPEPPVDDEIRARFQHLQ
SLIDKINNQLEQEARPGDFPNNISQSASDFKINYPEELNPQQLEAVTYCNGPLLIIAGAGTGKTRTLVYRVSYLLESGIP
PQEILLLTFTRKAAREMLERVKKLLGTESTDRIKGGTFHSFANNVLRRYSGLVGLSPDFSVLDQIDSQDVVDLLRTEYDF
HRQEKAFPKKERIFEIISKSRNTQKSIGQVISEEFSGLDDYIDEIDTIAQAYHKYKRHYNLMDFDDLLLVLRDNLRDNST
FRKQLSSELSYILVDEFQDTNVVQNEIVNLLGSQSRNITVVGDDFQSIYAFRGANWENILLFPQNYPDAKLIKLEKNYRS
HNKILKFANEIVDSAQIGYQKHLSSEFHEGTLPQFIKYYSQEEEAEAIVSEILALREQGFSYKDMAVLYRAGFHGNYIQA
ELLRRKIPYVVYGGIKFMERKHVKDLVSYLRLTVNPIDAVSWNRFLKMIPGIGRVRASQIVEEITEQDGRLNFNNFKGTS
YYKSLLSLQEVLLEAGKKGISPSNSVARIIEYYYPILEKQEVDASERKNDLEVLYHLSQNYSELEQFLADLSLEPPANKF
QQETTPALNEAEDDDSLVISTIHSAKGLEWNTVFIPHLLDGLFPSSKSLRDPFILEEERRLFYVAITRAKEKLYLSMPHQ
VVSYNGYFIQPSRFLKDLDPACYHFQAFFQDNKE

Sequences:

>Translated_754_residues
MIGDFSGSGNQNKGNGKDPLDKLIDFSKYKRRKQGQDKHEASNTEESLKNKETNHASIFPSQYPEPPVDDEIRARFQHLQ
SLIDKINNQLEQEARPGDFPNNISQSASDFKINYPEELNPQQLEAVTYCNGPLLIIAGAGTGKTRTLVYRVSYLLESGIP
PQEILLLTFTRKAAREMLERVKKLLGTESTDRIKGGTFHSFANNVLRRYSGLVGLSPDFSVLDQIDSQDVVDLLRTEYDF
HRQEKAFPKKERIFEIISKSRNTQKSIGQVISEEFSGLDDYIDEIDTIAQAYHKYKRHYNLMDFDDLLLVLRDNLRDNST
FRKQLSSELSYILVDEFQDTNVVQNEIVNLLGSQSRNITVVGDDFQSIYAFRGANWENILLFPQNYPDAKLIKLEKNYRS
HNKILKFANEIVDSAQIGYQKHLSSEFHEGTLPQFIKYYSQEEEAEAIVSEILALREQGFSYKDMAVLYRAGFHGNYIQA
ELLRRKIPYVVYGGIKFMERKHVKDLVSYLRLTVNPIDAVSWNRFLKMIPGIGRVRASQIVEEITEQDGRLNFNNFKGTS
YYKSLLSLQEVLLEAGKKGISPSNSVARIIEYYYPILEKQEVDASERKNDLEVLYHLSQNYSELEQFLADLSLEPPANKF
QQETTPALNEAEDDDSLVISTIHSAKGLEWNTVFIPHLLDGLFPSSKSLRDPFILEEERRLFYVAITRAKEKLYLSMPHQ
VVSYNGYFIQPSRFLKDLDPACYHFQAFFQDNKE
>Mature_754_residues
MIGDFSGSGNQNKGNGKDPLDKLIDFSKYKRRKQGQDKHEASNTEESLKNKETNHASIFPSQYPEPPVDDEIRARFQHLQ
SLIDKINNQLEQEARPGDFPNNISQSASDFKINYPEELNPQQLEAVTYCNGPLLIIAGAGTGKTRTLVYRVSYLLESGIP
PQEILLLTFTRKAAREMLERVKKLLGTESTDRIKGGTFHSFANNVLRRYSGLVGLSPDFSVLDQIDSQDVVDLLRTEYDF
HRQEKAFPKKERIFEIISKSRNTQKSIGQVISEEFSGLDDYIDEIDTIAQAYHKYKRHYNLMDFDDLLLVLRDNLRDNST
FRKQLSSELSYILVDEFQDTNVVQNEIVNLLGSQSRNITVVGDDFQSIYAFRGANWENILLFPQNYPDAKLIKLEKNYRS
HNKILKFANEIVDSAQIGYQKHLSSEFHEGTLPQFIKYYSQEEEAEAIVSEILALREQGFSYKDMAVLYRAGFHGNYIQA
ELLRRKIPYVVYGGIKFMERKHVKDLVSYLRLTVNPIDAVSWNRFLKMIPGIGRVRASQIVEEITEQDGRLNFNNFKGTS
YYKSLLSLQEVLLEAGKKGISPSNSVARIIEYYYPILEKQEVDASERKNDLEVLYHLSQNYSELEQFLADLSLEPPANKF
QQETTPALNEAEDDDSLVISTIHSAKGLEWNTVFIPHLLDGLFPSSKSLRDPFILEEERRLFYVAITRAKEKLYLSMPHQ
VVSYNGYFIQPSRFLKDLDPACYHFQAFFQDNKE

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=657, Percent_Identity=31.6590563165906, Blast_Score=278, Evalue=8e-76,
Organism=Escherichia coli, GI48994965, Length=652, Percent_Identity=32.361963190184, Blast_Score=278, Evalue=8e-76,
Organism=Escherichia coli, GI1787196, Length=335, Percent_Identity=28.955223880597, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=446, Percent_Identity=31.6143497757848, Blast_Score=165, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324477, Length=406, Percent_Identity=25.3694581280788, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 86852; Mature: 86852

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGDFSGSGNQNKGNGKDPLDKLIDFSKYKRRKQGQDKHEASNTEESLKNKETNHASIFP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCCCCCEECC
SQYPEPPVDDEIRARFQHLQSLIDKINNQLEQEARPGDFPNNISQSASDFKINYPEELNP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCEECCCCCCCH
QQLEAVTYCNGPLLIIAGAGTGKTRTLVYRVSYLLESGIPPQEILLLTFTRKAAREMLER
HHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHH
VKKLLGTESTDRIKGGTFHSFANNVLRRYSGLVGLSPDFSVLDQIDSQDVVDLLRTEYDF
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
HRQEKAFPKKERIFEIISKSRNTQKSIGQVISEEFSGLDDYIDEIDTIAQAYHKYKRHYN
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
LMDFDDLLLVLRDNLRDNSTFRKQLSSELSYILVDEFQDTNVVQNEIVNLLGSQSRNITV
CCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEE
VGDDFQSIYAFRGANWENILLFPQNYPDAKLIKLEKNYRSHNKILKFANEIVDSAQIGYQ
ECCCHHHHHHHCCCCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KHLSSEFHEGTLPQFIKYYSQEEEAEAIVSEILALREQGFSYKDMAVLYRAGFHGNYIQA
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHH
ELLRRKIPYVVYGGIKFMERKHVKDLVSYLRLTVNPIDAVSWNRFLKMIPGIGRVRASQI
HHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
VEEITEQDGRLNFNNFKGTSYYKSLLSLQEVLLEAGKKGISPSNSVARIIEYYYPILEKQ
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
EVDASERKNDLEVLYHLSQNYSELEQFLADLSLEPPANKFQQETTPALNEAEDDDSLVIS
CCCHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEE
TIHSAKGLEWNTVFIPHLLDGLFPSSKSLRDPFILEEERRLFYVAITRAKEKLYLSMPHQ
EHHHCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHHEEECCHH
VVSYNGYFIQPSRFLKDLDPACYHFQAFFQDNKE
HHCCCCEEECHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIGDFSGSGNQNKGNGKDPLDKLIDFSKYKRRKQGQDKHEASNTEESLKNKETNHASIFP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCCCCCEECC
SQYPEPPVDDEIRARFQHLQSLIDKINNQLEQEARPGDFPNNISQSASDFKINYPEELNP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCEECCCCCCCH
QQLEAVTYCNGPLLIIAGAGTGKTRTLVYRVSYLLESGIPPQEILLLTFTRKAAREMLER
HHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHH
VKKLLGTESTDRIKGGTFHSFANNVLRRYSGLVGLSPDFSVLDQIDSQDVVDLLRTEYDF
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
HRQEKAFPKKERIFEIISKSRNTQKSIGQVISEEFSGLDDYIDEIDTIAQAYHKYKRHYN
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
LMDFDDLLLVLRDNLRDNSTFRKQLSSELSYILVDEFQDTNVVQNEIVNLLGSQSRNITV
CCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEE
VGDDFQSIYAFRGANWENILLFPQNYPDAKLIKLEKNYRSHNKILKFANEIVDSAQIGYQ
ECCCHHHHHHHCCCCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KHLSSEFHEGTLPQFIKYYSQEEEAEAIVSEILALREQGFSYKDMAVLYRAGFHGNYIQA
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHH
ELLRRKIPYVVYGGIKFMERKHVKDLVSYLRLTVNPIDAVSWNRFLKMIPGIGRVRASQI
HHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
VEEITEQDGRLNFNNFKGTSYYKSLLSLQEVLLEAGKKGISPSNSVARIIEYYYPILEKQ
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
EVDASERKNDLEVLYHLSQNYSELEQFLADLSLEPPANKFQQETTPALNEAEDDDSLVIS
CCCHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEE
TIHSAKGLEWNTVFIPHLLDGLFPSSKSLRDPFILEEERRLFYVAITRAKEKLYLSMPHQ
EHHHCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHHEEECCHH
VVSYNGYFIQPSRFLKDLDPACYHFQAFFQDNKE
HHCCCCEEECHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]