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The map label for this gene is appA [H]

Identifier: 188587389

GI number: 188587389

Start: 3022756

End: 3024429

Strand: Reverse

Name: appA [H]

Synonym: Nther_2798

Alternate gene names: 188587389

Gene position: 3024429-3022756 (Counterclockwise)

Preceding gene: 188587390

Following gene: 188587388

Centisome position: 95.54

GC content: 39.67

Gene sequence:

>1674_bases
TTGCGAAAGCTGTTAATTTTACTGCTCGTAGTTTTTGTGGCGTCTTTTGGATTTATGGGTTGTGATCCGGCTGATCCTGA
AGAAATTGCTGAAGAAGAAGAAAAAGAGCCTGAAGAAGATGTTGACGAAGAAGAGGAAGAGCTAGAACAGGCTATTACTC
ATGCTATATGGAGTGAACCAGACGGACTATTTAATTATTGTGAATATGAAAGTACTTATGATCTTGATGCTTTTAATCAA
GTATTTGATGGCATGATGGAAGCAGATCCCCATGCAGATTTGGAACTAAATCCAAATTTAGCAGAAGAACATGAAATAAG
TGATGATGGAAAAACTTTTTATTACAAACTAAGAGATGATATTGAATTCCATGATGGTGAGCCACTAACTGCAGAAGATG
TTAAATTCACTTTCGAATGGATGTGTCATGAAGACTATATAGGTCCCAGAGCTAGTTACTGGCAGCACCTGGAAGGGTTT
GACGAATATCGAGCTGGAGAAGCTGACGAAGTAGAGGGAATTGAAATTATTAGCGACCATGAAATAGAATTCCACTTTGA
ACAAGTTGATGCTTCTGCCGTATATTATGTTAGTACCTGGGGCATTTCACCTAAACATGTATGGGAAGATATCCCTGTAG
GTGAAAGAAGAGAAGCCCCTGAAATGACAGAACCAATTGGAACAGGACCTTTTGAATTTGAGGAATACGTTGAAGGTCAA
TACGTAGAATTAGTTGCTAATGAAGATTATCACAGGGGAGAACCAGAATTAGAGAGAATTACTGTAGAAGTAAAAAGTCC
CGATGTAGTCAGGGCAGACTTGGAGACTGGTGAAGTTGATATCGCTGAAGTTCCACCTGATGAGAGCGAGTGGGATGATT
ATGAAGCTCACGATGAGTTAGCCCTTGAATATTATCCCACTAATGGTTATCAATACATGGGTATGAACTTAAGGGATGAA
AGTATCTTTAGTGATCATGCTGTAAGAGAGGCAGCTACTTACGCTATCAATAGGGAAGGTATGGTTGATGGTATCCTAGA
TGGTTTAGGTAAAGTCCAAAATTCCCACTTTTCACCTAACCAATGGGCTTATGACGAAGATTTAGATACTTATCCCCATG
ATCCTGATAAAGCCGAAGAAATATTAGAAGATGCTGGCTATACTAAAAACGATGATGGAATCTGGGAACAAAATGGTGAG
CCCTTAGAATTCACTCTATTATATCCTACCGGCGACGAACCCAGAGAACAAGCAGCTTTAATCATTGAACAGGACTTACA
GGGAATTGGTATTGACATTACTTTAGAATCTTTAGAATTTGCCACTTTGAGTGACAGGGTTTTTGACGAAATTGATTTCG
ATGCTTATCTTATGGGTTGGTCTTTAGGTGCAGACCCAGATCCAACGGGTATCTGGGGACCTGATGAAAGATTCAATGCT
GTTGGATTTGTACATCCGGAAAGCGACGAACTAATGGAGCAGGGTCTTCGTACTACAGATAGGGATGAACGAAGAGAACA
TTATGTAGAATGGCAGCGCTTACTTCAAGAAGAGATGCCTTATGTATTCTTGTACGCTGACTATGAAGGTTATGCCTACA
ACAATGATATCCAGGTATTTGAACCAAATCCTTGGAATATTTGGTATGACGTACATGAATGGTACCTTGAATAA

Upstream 100 bases:

>100_bases
ATATAAACTAGAGGGGGTGGTCAGACAAAAAGGCTGTTAGCTTGCTATCAGTTTAGACTAAATTGTTCGGAATGAAATTT
CAAATTTGGGGGGAAATTTA

Downstream 100 bases:

>100_bases
GTCATAAAGGTGTGATGAGGGGCCCCACCCTTTTTTGGGGTGGGGATCCTTATTTACGAAAGGAGGCGTGGAATGTATCA
ATATATAGTTAGAAGAATTC

Product: extracellular solute-binding protein family 5

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MRKLLILLLVVFVASFGFMGCDPADPEEIAEEEEKEPEEDVDEEEEELEQAITHAIWSEPDGLFNYCEYESTYDLDAFNQ
VFDGMMEADPHADLELNPNLAEEHEISDDGKTFYYKLRDDIEFHDGEPLTAEDVKFTFEWMCHEDYIGPRASYWQHLEGF
DEYRAGEADEVEGIEIISDHEIEFHFEQVDASAVYYVSTWGISPKHVWEDIPVGERREAPEMTEPIGTGPFEFEEYVEGQ
YVELVANEDYHRGEPELERITVEVKSPDVVRADLETGEVDIAEVPPDESEWDDYEAHDELALEYYPTNGYQYMGMNLRDE
SIFSDHAVREAATYAINREGMVDGILDGLGKVQNSHFSPNQWAYDEDLDTYPHDPDKAEEILEDAGYTKNDDGIWEQNGE
PLEFTLLYPTGDEPREQAALIIEQDLQGIGIDITLESLEFATLSDRVFDEIDFDAYLMGWSLGADPDPTGIWGPDERFNA
VGFVHPESDELMEQGLRTTDRDERREHYVEWQRLLQEEMPYVFLYADYEGYAYNNDIQVFEPNPWNIWYDVHEWYLE

Sequences:

>Translated_557_residues
MRKLLILLLVVFVASFGFMGCDPADPEEIAEEEEKEPEEDVDEEEEELEQAITHAIWSEPDGLFNYCEYESTYDLDAFNQ
VFDGMMEADPHADLELNPNLAEEHEISDDGKTFYYKLRDDIEFHDGEPLTAEDVKFTFEWMCHEDYIGPRASYWQHLEGF
DEYRAGEADEVEGIEIISDHEIEFHFEQVDASAVYYVSTWGISPKHVWEDIPVGERREAPEMTEPIGTGPFEFEEYVEGQ
YVELVANEDYHRGEPELERITVEVKSPDVVRADLETGEVDIAEVPPDESEWDDYEAHDELALEYYPTNGYQYMGMNLRDE
SIFSDHAVREAATYAINREGMVDGILDGLGKVQNSHFSPNQWAYDEDLDTYPHDPDKAEEILEDAGYTKNDDGIWEQNGE
PLEFTLLYPTGDEPREQAALIIEQDLQGIGIDITLESLEFATLSDRVFDEIDFDAYLMGWSLGADPDPTGIWGPDERFNA
VGFVHPESDELMEQGLRTTDRDERREHYVEWQRLLQEEMPYVFLYADYEGYAYNNDIQVFEPNPWNIWYDVHEWYLE
>Mature_557_residues
MRKLLILLLVVFVASFGFMGCDPADPEEIAEEEEKEPEEDVDEEEEELEQAITHAIWSEPDGLFNYCEYESTYDLDAFNQ
VFDGMMEADPHADLELNPNLAEEHEISDDGKTFYYKLRDDIEFHDGEPLTAEDVKFTFEWMCHEDYIGPRASYWQHLEGF
DEYRAGEADEVEGIEIISDHEIEFHFEQVDASAVYYVSTWGISPKHVWEDIPVGERREAPEMTEPIGTGPFEFEEYVEGQ
YVELVANEDYHRGEPELERITVEVKSPDVVRADLETGEVDIAEVPPDESEWDDYEAHDELALEYYPTNGYQYMGMNLRDE
SIFSDHAVREAATYAINREGMVDGILDGLGKVQNSHFSPNQWAYDEDLDTYPHDPDKAEEILEDAGYTKNDDGIWEQNGE
PLEFTLLYPTGDEPREQAALIIEQDLQGIGIDITLESLEFATLSDRVFDEIDFDAYLMGWSLGADPDPTGIWGPDERFNA
VGFVHPESDELMEQGLRTTDRDERREHYVEWQRLLQEEMPYVFLYADYEGYAYNNDIQVFEPNPWNIWYDVHEWYLE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=461, Percent_Identity=27.3318872017354, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1787551, Length=508, Percent_Identity=25.7874015748031, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789966, Length=454, Percent_Identity=22.9074889867841, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1789887, Length=375, Percent_Identity=28, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787052, Length=467, Percent_Identity=25.4817987152034, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64699; Mature: 64699

Theoretical pI: Translated: 3.70; Mature: 3.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLILLLVVFVASFGFMGCDPADPEEIAEEEEKEPEEDVDEEEEELEQAITHAIWSEP
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHCCC
DGLFNYCEYESTYDLDAFNQVFDGMMEADPHADLELNPNLAEEHEISDDGKTFYYKLRDD
CCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECC
IEFHDGEPLTAEDVKFTFEWMCHEDYIGPRASYWQHLEGFDEYRAGEADEVEGIEIISDH
CEECCCCCCCHHCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCCCCCCEEEEECC
EIEFHFEQVDASAVYYVSTWGISPKHVWEDIPVGERREAPEMTEPIGTGPFEFEEYVEGQ
CEEEEEEEECCCEEEEEEECCCCHHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCC
YVELVANEDYHRGEPELERITVEVKSPDVVRADLETGEVDIAEVPPDESEWDDYEAHDEL
EEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCE
ALEYYPTNGYQYMGMNLRDESIFSDHAVREAATYAINREGMVDGILDGLGKVQNSHFSPN
EEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
QWAYDEDLDTYPHDPDKAEEILEDAGYTKNDDGIWEQNGEPLEFTLLYPTGDEPREQAAL
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHH
IIEQDLQGIGIDITLESLEFATLSDRVFDEIDFDAYLMGWSLGADPDPTGIWGPDERFNA
HEECCCCCCCEEEEECCCEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCE
VGFVHPESDELMEQGLRTTDRDERREHYVEWQRLLQEEMPYVFLYADYEGYAYNNDIQVF
EEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCEEEE
EPNPWNIWYDVHEWYLE
CCCCCEEEEEHHHHHCC
>Mature Secondary Structure
MRKLLILLLVVFVASFGFMGCDPADPEEIAEEEEKEPEEDVDEEEEELEQAITHAIWSEP
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHCCC
DGLFNYCEYESTYDLDAFNQVFDGMMEADPHADLELNPNLAEEHEISDDGKTFYYKLRDD
CCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECC
IEFHDGEPLTAEDVKFTFEWMCHEDYIGPRASYWQHLEGFDEYRAGEADEVEGIEIISDH
CEECCCCCCCHHCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCCCCCCEEEEECC
EIEFHFEQVDASAVYYVSTWGISPKHVWEDIPVGERREAPEMTEPIGTGPFEFEEYVEGQ
CEEEEEEEECCCEEEEEEECCCCHHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCC
YVELVANEDYHRGEPELERITVEVKSPDVVRADLETGEVDIAEVPPDESEWDDYEAHDEL
EEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCE
ALEYYPTNGYQYMGMNLRDESIFSDHAVREAATYAINREGMVDGILDGLGKVQNSHFSPN
EEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
QWAYDEDLDTYPHDPDKAEEILEDAGYTKNDDGIWEQNGEPLEFTLLYPTGDEPREQAAL
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHH
IIEQDLQGIGIDITLESLEFATLSDRVFDEIDFDAYLMGWSLGADPDPTGIWGPDERFNA
HEECCCCCCCEEEEECCCEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCE
VGFVHPESDELMEQGLRTTDRDERREHYVEWQRLLQEEMPYVFLYADYEGYAYNNDIQVF
EEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCEEEE
EPNPWNIWYDVHEWYLE
CCCCCEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]