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The map label for this gene is buk1 [H]

Identifier: 188587297

GI number: 188587297

Start: 2913330

End: 2914388

Strand: Reverse

Name: buk1 [H]

Synonym: Nther_2700

Alternate gene names: 188587297

Gene position: 2914388-2913330 (Counterclockwise)

Preceding gene: 188587298

Following gene: 188587296

Centisome position: 92.07

GC content: 39.47

Gene sequence:

>1059_bases
TTGAAGATACTTGTTATAAATCCAGGTTCCACATCCACAAAAATTGCTTTATATGAAAATGAGACGGCAACAACTGAAAA
AACAGTTCGACATAGTGCCGATGAATTGGCTGAGTATCAAGATGTGGCAGATCAAGATGAATATCGGCTTAAGCATATAA
AGGAGACCCTGGAAGAAATTAATGTGGAATTAGAAGAACTTGATGCAGTCGTAGCTCGAGGAGGATTGTTAAGACCAATT
CCAGGTGGAACTTACAAAGTAACAGAAACCATGATAAATGATTTGAAACAAGCTAAATATGGAGAACACGCCTCAAATCT
AGGGGCTATTATGGCTGATATCCTATCAAAAGAACTGGATATTCCAGCTTATATTGTGGACCCTGTAGCTGTTGACGAGT
TTGAAGATATCGCCAGATATTCAGGTTTACCAGAATTAGAAAGATTAAGCCAATCCCATGCTTTAAATATGAAAGCCGTG
GCCCGTAAAGTAGCAAGGGAAATGGATCGCTCCTATGAAGATTTAAAACTAATTGTGGCTCATTTAGGAAGCGGTATTTC
GGTAGCTCCTCATTATAAAGGCCAGATGATTGATGTAAACAATGCCAACAACGAAGGCCCTTTTGCTACAGAAAGAACAG
GGACTCTCCCCGTATACCAGTTAATTCAATTGTGCTATTCAGGTAAATACACAGAAAAAGAAATGGTTGAAAGAGTTCTC
AAAAAAGGTGGAGTCTATGCCTATATGGGAACAAAAGATATCCAAGAAGTAGAAGAAAAGGCTAAATCAGGTGACGAACA
GGCAGATATGGTATTAAAAGCCATGACCTATCAAATAGCCAAGGAAATTGGTGCTATGGCTACTGTCCTAAATGGTGAAC
TGGATGCCATTGTCATAACTGGTGGTATGGCAAACTCTCAATATATAGTTGACCAAATAAAAGAAAGGGTTTCCTTTTTA
GGACCTGTAAAAGTTGAAGCTGGGGAAGAAGAGTTGGAGGCACTAGCCCTGGGGGCTTTAAGGGTACTCAACGGAGAAGA
AACTGCCAAGATTTATTAA

Upstream 100 bases:

>100_bases
TATAAAATGTTCGAACTTTAAAAATCTTATTATAGGTTCCTGGATCAGTCCAGGTCCAGGAACCTATAGGCATGTTAAAA
CCAAGGAAGGAGGATTATAA

Downstream 100 bases:

>100_bases
AATAAACAGTATTTAAGTAAAGTTAAAAGTCTGTATTTAGGAGGGGGAACTTAAAAATGAGAATTCAAAGTTTTTCGGAC
TTAACTGAACAGGTAAGAAA

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1 [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKILVINPGSTSTKIALYENETATTEKTVRHSADELAEYQDVADQDEYRLKHIKETLEEINVELEELDAVVARGGLLRPI
PGGTYKVTETMINDLKQAKYGEHASNLGAIMADILSKELDIPAYIVDPVAVDEFEDIARYSGLPELERLSQSHALNMKAV
ARKVAREMDRSYEDLKLIVAHLGSGISVAPHYKGQMIDVNNANNEGPFATERTGTLPVYQLIQLCYSGKYTEKEMVERVL
KKGGVYAYMGTKDIQEVEEKAKSGDEQADMVLKAMTYQIAKEIGAMATVLNGELDAIVITGGMANSQYIVDQIKERVSFL
GPVKVEAGEEELEALALGALRVLNGEETAKIY

Sequences:

>Translated_352_residues
MKILVINPGSTSTKIALYENETATTEKTVRHSADELAEYQDVADQDEYRLKHIKETLEEINVELEELDAVVARGGLLRPI
PGGTYKVTETMINDLKQAKYGEHASNLGAIMADILSKELDIPAYIVDPVAVDEFEDIARYSGLPELERLSQSHALNMKAV
ARKVAREMDRSYEDLKLIVAHLGSGISVAPHYKGQMIDVNNANNEGPFATERTGTLPVYQLIQLCYSGKYTEKEMVERVL
KKGGVYAYMGTKDIQEVEEKAKSGDEQADMVLKAMTYQIAKEIGAMATVLNGELDAIVITGGMANSQYIVDQIKERVSFL
GPVKVEAGEEELEALALGALRVLNGEETAKIY
>Mature_352_residues
MKILVINPGSTSTKIALYENETATTEKTVRHSADELAEYQDVADQDEYRLKHIKETLEEINVELEELDAVVARGGLLRPI
PGGTYKVTETMINDLKQAKYGEHASNLGAIMADILSKELDIPAYIVDPVAVDEFEDIARYSGLPELERLSQSHALNMKAV
ARKVAREMDRSYEDLKLIVAHLGSGISVAPHYKGQMIDVNNANNEGPFATERTGTLPVYQLIQLCYSGKYTEKEMVERVL
KKGGVYAYMGTKDIQEVEEKAKSGDEQADMVLKAMTYQIAKEIGAMATVLNGELDAIVITGGMANSQYIVDQIKERVSFL
GPVKVEAGEEELEALALGALRVLNGEETAKIY

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=176, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 38808; Mature: 38808

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS01075 ACETATE_KINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVINPGSTSTKIALYENETATTEKTVRHSADELAEYQDVADQDEYRLKHIKETLEEI
CEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NVELEELDAVVARGGLLRPIPGGTYKVTETMINDLKQAKYGEHASNLGAIMADILSKELD
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
IPAYIVDPVAVDEFEDIARYSGLPELERLSQSHALNMKAVARKVAREMDRSYEDLKLIVA
CCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHH
HLGSGISVAPHYKGQMIDVNNANNEGPFATERTGTLPVYQLIQLCYSGKYTEKEMVERVL
HHCCCCEECCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
KKGGVYAYMGTKDIQEVEEKAKSGDEQADMVLKAMTYQIAKEIGAMATVLNGELDAIVIT
HHCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GGMANSQYIVDQIKERVSFLGPVKVEAGEEELEALALGALRVLNGEETAKIY
CCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKILVINPGSTSTKIALYENETATTEKTVRHSADELAEYQDVADQDEYRLKHIKETLEEI
CEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NVELEELDAVVARGGLLRPIPGGTYKVTETMINDLKQAKYGEHASNLGAIMADILSKELD
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
IPAYIVDPVAVDEFEDIARYSGLPELERLSQSHALNMKAVARKVAREMDRSYEDLKLIVA
CCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHH
HLGSGISVAPHYKGQMIDVNNANNEGPFATERTGTLPVYQLIQLCYSGKYTEKEMVERVL
HHCCCCEECCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
KKGGVYAYMGTKDIQEVEEKAKSGDEQADMVLKAMTYQIAKEIGAMATVLNGELDAIVIT
HHCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GGMANSQYIVDQIKERVSFLGPVKVEAGEEELEALALGALRVLNGEETAKIY
CCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]