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The map label for this gene is glpK

Identifier: 188587273

GI number: 188587273

Start: 2882264

End: 2883760

Strand: Reverse

Name: glpK

Synonym: Nther_2675

Alternate gene names: 188587273

Gene position: 2883760-2882264 (Counterclockwise)

Preceding gene: 188587274

Following gene: 188587272

Centisome position: 91.1

GC content: 41.48

Gene sequence:

>1497_bases
ATGCAAAATTATGTTATATCACTAGATCAAGGAACTACCAGTTCTAGAGCCATTCTCTACGACCAGCAGGGTAATATAGT
TAAATCGGCTCAAAAAGAAATTACCCAGCACTATCCACAATCGAGCTGGGTAGAGCATGACGCTAGCGAAATATGGGGGA
CACAAAGTGGAGTACTGAGAGAGGTCCTAGAGACAGCCGGGATAAGACCGAATCAAATTGCAGCAATTGGTATTGCCAAT
CAACGTGAAACTACAGTCATTTGGGATAAAGAATCTGGGAAACCAATTCATAATGCCATTGTTTGGCAGGATAGAAGGAC
GGCACCTATTTGTGAAGAACTTGAGAAACAGGGGTTGAAAGAATACATAAGACAGAACACTGGTTTGGTTATTGATGCTT
ATTTTTCAGCGACAAAAGTTAAGTGGCTTCTGGATAATGTTAATGGGGCGCGAGAGAAAGCCAAGAATGGGCAATTGCTG
TTTGGTACTGTGGATAGCTGGTTGATTTGGAATTTAACCCGGGGTCATGTTCATGTCACTGATTATACTAATGCTTCCAG
AACCATGATGTTTAATATTCATGAACTGGATTGGGATGACAAAATTCTTGATGTTTTAGACATACCTAGAGAAATGCTGC
CAGAAATTAAGGAATCAAGTGAAATTTATGGTTATACCGATGAACATACCCTGGGTGGTGCTCAAATTCCGATATCCGGG
ATAGCTGGTGATCAGCAATCTGCTTTATTTGGACAAGGCTGTTTTGAAAGAGGTATGATGAAAAATACTTACGGTACTGG
ATGTTTTTTGCTTATGAACACAGGCAAAGACCCTGTTATTTCGAAAAATGGACTTTTGACTACTATAGCATGGGCCATAG
ATGGTGAAATCTATTATGCTCTGGAAGGCAGTATTTTTATTGCCGGTGCGGCCGTTCAGTGGTTGAGAGACAATTTAAAA
TTGATAGATACTGCTCATGATTCGGAATACTTTGCAGGGAAAAGTGACGAGAGTAATGGTGTTTATGTGGTACCGGCTTT
TCAGGGTCTTGGTGCTCCTTACTGGGATATGTATGCTAGAGGCGCTATTATGGGATTAACTCGCAAAGTTGGTAAAGCCG
AAATTGTTCGGGCTACCCTGGAATCTCTAGCATATCAAACCCGGGATGTGGTAGATGCTATGAAAAAAGATTCCCAACTC
CAATTACAGACTTTACGGGTTGATGGAGGAGCATGCCAAAATAACCTTTTGATGCAATTTCAATCCGATATAATTGGGAC
TACTGTGGAACGCCCTTCAGATATAGAAACCACTGCTCGTGGTGCTGCTTTTTTGGCAGGTTTAGCTGTAGGTTTCTGGG
ATAAGTTTGATCTCCAAAAAGTTCAGCAAATAGATAACAAATTTACACCTGAAATGGACTCCCAAGAAAGAGAAAATAGG
TATGCGGGCTGGCAAAAAGCTGTTGAGAGGACCATGGGCTGGGCTCGTGATAGTTAA

Upstream 100 bases:

>100_bases
AAGAGAAAAATTGTGTGCATAACAATGGGAGAGGGGGATGTCAGTTAATTAATAAATAATAGTCGAAACATTAATTATTT
ATATTTGGGGGAAGGTATTT

Downstream 100 bases:

>100_bases
TTTAATGCCTTAATGTATGGATCAACGATCATGTACTGTCAAGCTCCGTATTATTTATGCCTAAAACTACCATTATTTAT
GTTTGAGATTACATAAAAAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MQNYVISLDQGTTSSRAILYDQQGNIVKSAQKEITQHYPQSSWVEHDASEIWGTQSGVLREVLETAGIRPNQIAAIGIAN
QRETTVIWDKESGKPIHNAIVWQDRRTAPICEELEKQGLKEYIRQNTGLVIDAYFSATKVKWLLDNVNGAREKAKNGQLL
FGTVDSWLIWNLTRGHVHVTDYTNASRTMMFNIHELDWDDKILDVLDIPREMLPEIKESSEIYGYTDEHTLGGAQIPISG
IAGDQQSALFGQGCFERGMMKNTYGTGCFLLMNTGKDPVISKNGLLTTIAWAIDGEIYYALEGSIFIAGAAVQWLRDNLK
LIDTAHDSEYFAGKSDESNGVYVVPAFQGLGAPYWDMYARGAIMGLTRKVGKAEIVRATLESLAYQTRDVVDAMKKDSQL
QLQTLRVDGGACQNNLLMQFQSDIIGTTVERPSDIETTARGAAFLAGLAVGFWDKFDLQKVQQIDNKFTPEMDSQERENR
YAGWQKAVERTMGWARDS

Sequences:

>Translated_498_residues
MQNYVISLDQGTTSSRAILYDQQGNIVKSAQKEITQHYPQSSWVEHDASEIWGTQSGVLREVLETAGIRPNQIAAIGIAN
QRETTVIWDKESGKPIHNAIVWQDRRTAPICEELEKQGLKEYIRQNTGLVIDAYFSATKVKWLLDNVNGAREKAKNGQLL
FGTVDSWLIWNLTRGHVHVTDYTNASRTMMFNIHELDWDDKILDVLDIPREMLPEIKESSEIYGYTDEHTLGGAQIPISG
IAGDQQSALFGQGCFERGMMKNTYGTGCFLLMNTGKDPVISKNGLLTTIAWAIDGEIYYALEGSIFIAGAAVQWLRDNLK
LIDTAHDSEYFAGKSDESNGVYVVPAFQGLGAPYWDMYARGAIMGLTRKVGKAEIVRATLESLAYQTRDVVDAMKKDSQL
QLQTLRVDGGACQNNLLMQFQSDIIGTTVERPSDIETTARGAAFLAGLAVGFWDKFDLQKVQQIDNKFTPEMDSQERENR
YAGWQKAVERTMGWARDS
>Mature_498_residues
MQNYVISLDQGTTSSRAILYDQQGNIVKSAQKEITQHYPQSSWVEHDASEIWGTQSGVLREVLETAGIRPNQIAAIGIAN
QRETTVIWDKESGKPIHNAIVWQDRRTAPICEELEKQGLKEYIRQNTGLVIDAYFSATKVKWLLDNVNGAREKAKNGQLL
FGTVDSWLIWNLTRGHVHVTDYTNASRTMMFNIHELDWDDKILDVLDIPREMLPEIKESSEIYGYTDEHTLGGAQIPISG
IAGDQQSALFGQGCFERGMMKNTYGTGCFLLMNTGKDPVISKNGLLTTIAWAIDGEIYYALEGSIFIAGAAVQWLRDNLK
LIDTAHDSEYFAGKSDESNGVYVVPAFQGLGAPYWDMYARGAIMGLTRKVGKAEIVRATLESLAYQTRDVVDAMKKDSQL
QLQTLRVDGGACQNNLLMQFQSDIIGTTVERPSDIETTARGAAFLAGLAVGFWDKFDLQKVQQIDNKFTPEMDSQERENR
YAGWQKAVERTMGWARDS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=46.4285714285714, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=46.4285714285714, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI42794763, Length=508, Percent_Identity=45.8661417322835, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI41393575, Length=500, Percent_Identity=46.2, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=35.6299212598425, Blast_Score=351, Evalue=7e-97,
Organism=Escherichia coli, GI1790361, Length=494, Percent_Identity=66.3967611336032, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1789987, Length=497, Percent_Identity=24.5472837022133, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790005, Length=510, Percent_Identity=23.3333333333333, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787789, Length=517, Percent_Identity=24.5647969052224, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789168, Length=474, Percent_Identity=23.2067510548523, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=503, Percent_Identity=41.948310139165, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=27.2552783109405, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=31.3333333333333, Blast_Score=158, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321755, Length=560, Percent_Identity=35.1785714285714, Blast_Score=307, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17864214, Length=509, Percent_Identity=42.6326129666012, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655555, Length=480, Percent_Identity=41.4583333333333, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24655560, Length=480, Percent_Identity=41.4583333333333, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24655570, Length=480, Percent_Identity=41.4583333333333, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24655574, Length=480, Percent_Identity=41.4583333333333, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24655565, Length=480, Percent_Identity=41.4583333333333, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24654573, Length=452, Percent_Identity=44.2477876106195, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI19922058, Length=530, Percent_Identity=33.7735849056604, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24652871, Length=497, Percent_Identity=34.8088531187123, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24656367, Length=515, Percent_Identity=28.7378640776699, Blast_Score=231, Evalue=9e-61,
Organism=Drosophila melanogaster, GI21357867, Length=532, Percent_Identity=28.1954887218045, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=29.512893982808, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI45552909, Length=286, Percent_Identity=27.2727272727273, Blast_Score=125, Evalue=9e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_NATTJ (B2A2C4)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918818.1
- ProteinModelPortal:   B2A2C4
- SMR:   B2A2C4
- GeneID:   6314502
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2675
- HOGENOM:   HBG511469
- OMA:   IQRSADV
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55707; Mature: 55707

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNYVISLDQGTTSSRAILYDQQGNIVKSAQKEITQHYPQSSWVEHDASEIWGTQSGVLR
CCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHCCCHHHHCCCHHHHHH
EVLETAGIRPNQIAAIGIANQRETTVIWDKESGKPIHNAIVWQDRRTAPICEELEKQGLK
HHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHEEEECCCCCCHHHHHHHHHHHH
EYIRQNTGLVIDAYFSATKVKWLLDNVNGAREKAKNGQLLFGTVDSWLIWNLTRGHVHVT
HHHHCCCCEEEEEECCHHHHHHHHHCCCHHHHHCCCCCEEEEECCCEEEEEEECCEEEEE
DYTNASRTMMFNIHELDWDDKILDVLDIPREMLPEIKESSEIYGYTDEHTLGGAQIPISG
ECCCCCEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCEECCCCCCCCCCCCCEECC
IAGDQQSALFGQGCFERGMMKNTYGTGCFLLMNTGKDPVISKNGLLTTIAWAIDGEIYYA
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEECCCCEEEEEEEECCEEEEE
LEGSIFIAGAAVQWLRDNLKLIDTAHDSEYFAGKSDESNGVYVVPAFQGLGAPYWDMYAR
EECCEEEEHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHH
GAIMGLTRKVGKAEIVRATLESLAYQTRDVVDAMKKDSQLQLQTLRVDGGACQNNLLMQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHH
QSDIIGTTVERPSDIETTARGAAFLAGLAVGFWDKFDLQKVQQIDNKFTPEMDSQERENR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
YAGWQKAVERTMGWARDS
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQNYVISLDQGTTSSRAILYDQQGNIVKSAQKEITQHYPQSSWVEHDASEIWGTQSGVLR
CCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHCCCHHHHCCCHHHHHH
EVLETAGIRPNQIAAIGIANQRETTVIWDKESGKPIHNAIVWQDRRTAPICEELEKQGLK
HHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHEEEECCCCCCHHHHHHHHHHHH
EYIRQNTGLVIDAYFSATKVKWLLDNVNGAREKAKNGQLLFGTVDSWLIWNLTRGHVHVT
HHHHCCCCEEEEEECCHHHHHHHHHCCCHHHHHCCCCCEEEEECCCEEEEEEECCEEEEE
DYTNASRTMMFNIHELDWDDKILDVLDIPREMLPEIKESSEIYGYTDEHTLGGAQIPISG
ECCCCCEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCEECCCCCCCCCCCCCEECC
IAGDQQSALFGQGCFERGMMKNTYGTGCFLLMNTGKDPVISKNGLLTTIAWAIDGEIYYA
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEECCCCEEEEEEEECCEEEEE
LEGSIFIAGAAVQWLRDNLKLIDTAHDSEYFAGKSDESNGVYVVPAFQGLGAPYWDMYAR
EECCEEEEHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHH
GAIMGLTRKVGKAEIVRATLESLAYQTRDVVDAMKKDSQLQLQTLRVDGGACQNNLLMQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHH
QSDIIGTTVERPSDIETTARGAAFLAGLAVGFWDKFDLQKVQQIDNKFTPEMDSQERENR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
YAGWQKAVERTMGWARDS
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA