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The map label for this gene is mleN [H]

Identifier: 188587269

GI number: 188587269

Start: 2878515

End: 2879939

Strand: Direct

Name: mleN [H]

Synonym: Nther_2671

Alternate gene names: 188587269

Gene position: 2878515-2879939 (Clockwise)

Preceding gene: 188587246

Following gene: 188587275

Centisome position: 90.93

GC content: 39.44

Gene sequence:

>1425_bases
TTGTCTGATCAGCAAAATAATTATAATGAGCATCAGGAAACAATTAGGCCTACTACTGCTCAGGCCTTCACTCCAATGAT
AGCTTTGGTAGTGTTAATAGCTTTTAGTGCCATTGTTTTAGGAGTAGATATTCACATTCCATTGTTTCTGTCTACTGTCA
TTGCAGCTGTAGTAGCCGTTGCTTGGATGGGACATAATTGGGAAACAGTAGAAGAAGGTATACTAAAAGGTATTAACTTT
GCTTTAGGTGCGGTAATAATCTTAGCAATTGTAGGGATTTTAATTGGAAGCTGGATTGAAGGTGGAATAGTACCCACTAT
TGTTTATTACGGACTTCAGTTATTATCTCCACAAATTTTCTTAGTGGCGACCCTAGTTATAACAGCAGTAGTTTCTGTTT
TCACCGGTAGTTCCTGGTCATCAATTGCTACAATTGGACTAGCTTTAGCTGGGATTGGCCAGGGACTTGGAATGCCCCCT
GCCATGACTGTAGGTGCTATTGTTTCCGGTGCTTACTTTGGGGACAAGTTATCTCCTTTATCTGATACAACCGTTGTTGC
ATCTGGTACTGCTGGAGTTAATATTTATGACCATATTAAACACATGTTGTACGTGTCGGGTCCAGCTTTTGTAATATCAG
CAGTTATTTTTGGCATCCTAGGATTTCAGTTTGCAGGAGAAGAACTGGACTATGGTGAGATAGGAGAGCTCACGGGTACA
ATTGCCGAGCATTTCAATGTAGGTCCACTGATGTTTTTACCGATAATTGCAGTACTTATCATGATCTTGTTTAGAGTACC
ACCTATCCCTGCTTTGACTGGTGGTTGCATCCTTGGTTTCATTTGGGCTGTAGTCTTTCAAGGAGCTGGTTTGGCAGACA
TATTTGGCTCTATGAATTATGGTTATGTCATGGATAGTGGAATAGAAACCATAGATGAATTATTTACAGCCGGTGGGTTG
CAAAGCATGATGTGGACTATTTCATTGATTCTAGTGGCATTAGCCTTCGGTGGAATTGTAGAACATACCAAAATGATGGA
AGTAATGCTGGAAAAAGTTTTAGCTGTAGCGACAACTCAAAAGAAACTTGCTTTTGCAGCACATTTAACCACTCTATTTT
TAATTCTGACAGTTAACTCTCATTACCTAACTCATGTATTGACTTGTAGATCTTATAAACATGCTTTCGAAGAATCCGGC
TTACATGCTAAGAACATTTCTCGAATTTCTGAAGCATGGGGGACACTGCCTTCTTCCTTAATCCCTTGGAACCCTTGCGG
AGCTTTTGTTTATGGTACTTTAGGCGTTTATCCCTTTGCTTATCTTCCTTTTGCTTTTTTCATTTTAATTGCTATGGTGA
TATCCTTGATCTATGGATTTACAGGATTTTCTATGGAGCAGTTACATGAAAAAGAAGCATCCTAA

Upstream 100 bases:

>100_bases
AAACAATTGACTTCTGATGCATTTTCATTATTACATTTTTTTAAAATTTAATTAAAAATTGAAAACCTAACATTAATATT
ATTATTTAGGAGGGTTAATC

Downstream 100 bases:

>100_bases
AAGGTATTAGCAGTTATTTACAATTAAATTAACCCCGGCTAGGAGGAGCTAGTGAAAAGTTACGAATAGTAACTAGCTCT
AAAACCGGCCGGGGTTTTTT

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSDQQNNYNEHQETIRPTTAQAFTPMIALVVLIAFSAIVLGVDIHIPLFLSTVIAAVVAVAWMGHNWETVEEGILKGINF
ALGAVIILAIVGILIGSWIEGGIVPTIVYYGLQLLSPQIFLVATLVITAVVSVFTGSSWSSIATIGLALAGIGQGLGMPP
AMTVGAIVSGAYFGDKLSPLSDTTVVASGTAGVNIYDHIKHMLYVSGPAFVISAVIFGILGFQFAGEELDYGEIGELTGT
IAEHFNVGPLMFLPIIAVLIMILFRVPPIPALTGGCILGFIWAVVFQGAGLADIFGSMNYGYVMDSGIETIDELFTAGGL
QSMMWTISLILVALAFGGIVEHTKMMEVMLEKVLAVATTQKKLAFAAHLTTLFLILTVNSHYLTHVLTCRSYKHAFEESG
LHAKNISRISEAWGTLPSSLIPWNPCGAFVYGTLGVYPFAYLPFAFFILIAMVISLIYGFTGFSMEQLHEKEAS

Sequences:

>Translated_474_residues
MSDQQNNYNEHQETIRPTTAQAFTPMIALVVLIAFSAIVLGVDIHIPLFLSTVIAAVVAVAWMGHNWETVEEGILKGINF
ALGAVIILAIVGILIGSWIEGGIVPTIVYYGLQLLSPQIFLVATLVITAVVSVFTGSSWSSIATIGLALAGIGQGLGMPP
AMTVGAIVSGAYFGDKLSPLSDTTVVASGTAGVNIYDHIKHMLYVSGPAFVISAVIFGILGFQFAGEELDYGEIGELTGT
IAEHFNVGPLMFLPIIAVLIMILFRVPPIPALTGGCILGFIWAVVFQGAGLADIFGSMNYGYVMDSGIETIDELFTAGGL
QSMMWTISLILVALAFGGIVEHTKMMEVMLEKVLAVATTQKKLAFAAHLTTLFLILTVNSHYLTHVLTCRSYKHAFEESG
LHAKNISRISEAWGTLPSSLIPWNPCGAFVYGTLGVYPFAYLPFAFFILIAMVISLIYGFTGFSMEQLHEKEAS
>Mature_473_residues
SDQQNNYNEHQETIRPTTAQAFTPMIALVVLIAFSAIVLGVDIHIPLFLSTVIAAVVAVAWMGHNWETVEEGILKGINFA
LGAVIILAIVGILIGSWIEGGIVPTIVYYGLQLLSPQIFLVATLVITAVVSVFTGSSWSSIATIGLALAGIGQGLGMPPA
MTVGAIVSGAYFGDKLSPLSDTTVVASGTAGVNIYDHIKHMLYVSGPAFVISAVIFGILGFQFAGEELDYGEIGELTGTI
AEHFNVGPLMFLPIIAVLIMILFRVPPIPALTGGCILGFIWAVVFQGAGLADIFGSMNYGYVMDSGIETIDELFTAGGLQ
SMMWTISLILVALAFGGIVEHTKMMEVMLEKVLAVATTQKKLAFAAHLTTLFLILTVNSHYLTHVLTCRSYKHAFEESGL
HAKNISRISEAWGTLPSSLIPWNPCGAFVYGTLGVYPFAYLPFAFFILIAMVISLIYGFTGFSMEQLHEKEAS

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 50713; Mature: 50582

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQQNNYNEHQETIRPTTAQAFTPMIALVVLIAFSAIVLGVDIHIPLFLSTVIAAVVAV
CCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWMGHNWETVEEGILKGINFALGAVIILAIVGILIGSWIEGGIVPTIVYYGLQLLSPQIF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
LVATLVITAVVSVFTGSSWSSIATIGLALAGIGQGLGMPPAMTVGAIVSGAYFGDKLSPL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SDTTVVASGTAGVNIYDHIKHMLYVSGPAFVISAVIFGILGFQFAGEELDYGEIGELTGT
CCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IAEHFNVGPLMFLPIIAVLIMILFRVPPIPALTGGCILGFIWAVVFQGAGLADIFGSMNY
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
GYVMDSGIETIDELFTAGGLQSMMWTISLILVALAFGGIVEHTKMMEVMLEKVLAVATTQ
CCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLAFAAHLTTLFLILTVNSHYLTHVLTCRSYKHAFEESGLHAKNISRISEAWGTLPSSL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
IPWNPCGAFVYGTLGVYPFAYLPFAFFILIAMVISLIYGFTGFSMEQLHEKEAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SDQQNNYNEHQETIRPTTAQAFTPMIALVVLIAFSAIVLGVDIHIPLFLSTVIAAVVAV
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWMGHNWETVEEGILKGINFALGAVIILAIVGILIGSWIEGGIVPTIVYYGLQLLSPQIF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHH
LVATLVITAVVSVFTGSSWSSIATIGLALAGIGQGLGMPPAMTVGAIVSGAYFGDKLSPL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SDTTVVASGTAGVNIYDHIKHMLYVSGPAFVISAVIFGILGFQFAGEELDYGEIGELTGT
CCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IAEHFNVGPLMFLPIIAVLIMILFRVPPIPALTGGCILGFIWAVVFQGAGLADIFGSMNY
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
GYVMDSGIETIDELFTAGGLQSMMWTISLILVALAFGGIVEHTKMMEVMLEKVLAVATTQ
CCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLAFAAHLTTLFLILTVNSHYLTHVLTCRSYKHAFEESGLHAKNISRISEAWGTLPSSL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
IPWNPCGAFVYGTLGVYPFAYLPFAFFILIAMVISLIYGFTGFSMEQLHEKEAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]