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The map label for this gene is dcuB [H]

Identifier: 188587255

GI number: 188587255

Start: 2861579

End: 2862880

Strand: Reverse

Name: dcuB [H]

Synonym: Nther_2657

Alternate gene names: 188587255

Gene position: 2862880-2861579 (Counterclockwise)

Preceding gene: 188587256

Following gene: 188587254

Centisome position: 90.44

GC content: 40.02

Gene sequence:

>1302_bases
GTGTTATTCGTACAATTTGCTATCGTACTGTTGGCGATTTTGGTTGGTGCACGAATTGGTGGAATAGGTCTAGGTGCTAT
GGGTGGCGTAGGTCTAGCCATTATGACTTTTATATTCAATTTAGAACCTACATCACCACCGATTAATGTAATGTTGATGA
TAGTAGCTGTTGTAACTGCTGCTTCAACTCTTCAAGCAGCTGGAGGTATGGACTATTTAGTAAAATTAGCAGAACGCGCA
TTACGCAAAAAACCTGATTTAATTACTTTTATGGCTCCAGTAATCACGTATACATTCACATTCTTCGCAGGAACAGGACA
CGTTGCTTATTCCTTGCTACCTGTTATTTCCGAGGTCTCTCATGAGCAGAAGGTACGGCCTGAAAGGCCTTTATCAATAG
CAGTTATTGCTTCACAACAAGCTATTGTAGCCAGTCCTATCTCAGCGGCAACAGTTGCTCTGGTAGGGATACTTTCACCT
CATGGATTTACTTTGCCTAATATTTTAAGTGTTACAATTCCTGCAACTTTAATAGCTATTTTATTCACGGCCTTTATGAT
CCGAAAGAAAGGTCCTGAATTAGAAGATGATCCAGAATATCAGAAACGATTACAGGAAGGTATCAAACCATTAAGCAAAG
ATGACACCGAAACAGAAATTACCAGTTCAGCTAAATTATCAGTAGGACTTTTCCTATTGGCAGCTGTAGCAGTAGTATTT
TTCGGAACATTTGAGGGACTAAGACCAGCTTTTGATGGTGAACCAATGTCAATGCCACATACCATTCAAATTATCATGTT
GACAGTTGCCGCTTTACACTTGCTTGTCTGTAAAGCTGAAGTTGATGTTGCTGCAAATGGCAGTGTGTTTAGGGCAGGTT
TCGTTGCCGTGGTTGCTATCTTTGGAATCGCCTGGTTAGGCGATACATTTTTTGAAGCTCACATGGATGTAATTGAACCC
GCTGTGGAAGGAATGGTAACTACAGCACCATGGGTCTTTGCCTTTGCACTATTTATTATGTCAATTTTACTGAACAGTCA
GGCAGCTACTACAACTACTTTAATGCCCCTTGGACTAGCCTTGGGTATTAATCCAGCATTCTTAATTGCTATGTTCCCAG
CAGTCAATGGATATTTCTTCCTACCAAATTACGGCCCAATTATTGCAGCTATTGGTTTTGACCGCACTGGTACTACTAAA
ATTGGCAAATTTGTCTTTAACCACAGCTTTATGATTCCAGGATTGTTAACTATTGTGGGATCATTAGTTCTAGGTTTCCT
ATTATCATCCATTATCTTGTAA

Upstream 100 bases:

>100_bases
TAAGTTAAGCGTATTTACAAGTTTTTCTAGGTAATTTTTTTCATCAAGATGTGAAAGGGATAACAAAAACGATTATGAAT
AATAAAAGGAAGGTGTTACA

Downstream 100 bases:

>100_bases
GGATTTAAAACTTAAAGAATATAAATAGAGAGGGACCCTGATGATGGGGTCCTCTCTCTATATATAACCATTTATCTAAG
TATAAAAGTTTATATTATAA

Product: anaerobic c4-dicarboxylate antiporter, Dcu family

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLFVQFAIVLLAILVGARIGGIGLGAMGGVGLAIMTFIFNLEPTSPPINVMLMIVAVVTAASTLQAAGGMDYLVKLAERA
LRKKPDLITFMAPVITYTFTFFAGTGHVAYSLLPVISEVSHEQKVRPERPLSIAVIASQQAIVASPISAATVALVGILSP
HGFTLPNILSVTIPATLIAILFTAFMIRKKGPELEDDPEYQKRLQEGIKPLSKDDTETEITSSAKLSVGLFLLAAVAVVF
FGTFEGLRPAFDGEPMSMPHTIQIIMLTVAALHLLVCKAEVDVAANGSVFRAGFVAVVAIFGIAWLGDTFFEAHMDVIEP
AVEGMVTTAPWVFAFALFIMSILLNSQAATTTTLMPLGLALGINPAFLIAMFPAVNGYFFLPNYGPIIAAIGFDRTGTTK
IGKFVFNHSFMIPGLLTIVGSLVLGFLLSSIIL

Sequences:

>Translated_433_residues
MLFVQFAIVLLAILVGARIGGIGLGAMGGVGLAIMTFIFNLEPTSPPINVMLMIVAVVTAASTLQAAGGMDYLVKLAERA
LRKKPDLITFMAPVITYTFTFFAGTGHVAYSLLPVISEVSHEQKVRPERPLSIAVIASQQAIVASPISAATVALVGILSP
HGFTLPNILSVTIPATLIAILFTAFMIRKKGPELEDDPEYQKRLQEGIKPLSKDDTETEITSSAKLSVGLFLLAAVAVVF
FGTFEGLRPAFDGEPMSMPHTIQIIMLTVAALHLLVCKAEVDVAANGSVFRAGFVAVVAIFGIAWLGDTFFEAHMDVIEP
AVEGMVTTAPWVFAFALFIMSILLNSQAATTTTLMPLGLALGINPAFLIAMFPAVNGYFFLPNYGPIIAAIGFDRTGTTK
IGKFVFNHSFMIPGLLTIVGSLVLGFLLSSIIL
>Mature_433_residues
MLFVQFAIVLLAILVGARIGGIGLGAMGGVGLAIMTFIFNLEPTSPPINVMLMIVAVVTAASTLQAAGGMDYLVKLAERA
LRKKPDLITFMAPVITYTFTFFAGTGHVAYSLLPVISEVSHEQKVRPERPLSIAVIASQQAIVASPISAATVALVGILSP
HGFTLPNILSVTIPATLIAILFTAFMIRKKGPELEDDPEYQKRLQEGIKPLSKDDTETEITSSAKLSVGLFLLAAVAVVF
FGTFEGLRPAFDGEPMSMPHTIQIIMLTVAALHLLVCKAEVDVAANGSVFRAGFVAVVAIFGIAWLGDTFFEAHMDVIEP
AVEGMVTTAPWVFAFALFIMSILLNSQAATTTTLMPLGLALGINPAFLIAMFPAVNGYFFLPNYGPIIAAIGFDRTGTTK
IGKFVFNHSFMIPGLLTIVGSLVLGFLLSSIIL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=435, Percent_Identity=45.9770114942529, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1790565, Length=405, Percent_Identity=40.2469135802469, Blast_Score=308, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 46021; Mature: 46021

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFVQFAIVLLAILVGARIGGIGLGAMGGVGLAIMTFIFNLEPTSPPINVMLMIVAVVTA
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ASTLQAAGGMDYLVKLAERALRKKPDLITFMAPVITYTFTFFAGTGHVAYSLLPVISEVS
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
HEQKVRPERPLSIAVIASQQAIVASPISAATVALVGILSPHGFTLPNILSVTIPATLIAI
HHHCCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LFTAFMIRKKGPELEDDPEYQKRLQEGIKPLSKDDTETEITSSAKLSVGLFLLAAVAVVF
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
FGTFEGLRPAFDGEPMSMPHTIQIIMLTVAALHLLVCKAEVDVAANGSVFRAGFVAVVAI
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FGIAWLGDTFFEAHMDVIEPAVEGMVTTAPWVFAFALFIMSILLNSQAATTTTLMPLGLA
HHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LGINPAFLIAMFPAVNGYFFLPNYGPIIAAIGFDRTGTTKIGKFVFNHSFMIPGLLTIVG
HCCCHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHH
SLVLGFLLSSIIL
HHHHHHHHHHHCC
>Mature Secondary Structure
MLFVQFAIVLLAILVGARIGGIGLGAMGGVGLAIMTFIFNLEPTSPPINVMLMIVAVVTA
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ASTLQAAGGMDYLVKLAERALRKKPDLITFMAPVITYTFTFFAGTGHVAYSLLPVISEVS
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
HEQKVRPERPLSIAVIASQQAIVASPISAATVALVGILSPHGFTLPNILSVTIPATLIAI
HHHCCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LFTAFMIRKKGPELEDDPEYQKRLQEGIKPLSKDDTETEITSSAKLSVGLFLLAAVAVVF
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
FGTFEGLRPAFDGEPMSMPHTIQIIMLTVAALHLLVCKAEVDVAANGSVFRAGFVAVVAI
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FGIAWLGDTFFEAHMDVIEPAVEGMVTTAPWVFAFALFIMSILLNSQAATTTTLMPLGLA
HHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LGINPAFLIAMFPAVNGYFFLPNYGPIIAAIGFDRTGTTKIGKFVFNHSFMIPGLLTIVG
HCCCHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHH
SLVLGFLLSSIIL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]