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The map label for this gene is dppA [H]

Identifier: 188587243

GI number: 188587243

Start: 2842824

End: 2844422

Strand: Direct

Name: dppA [H]

Synonym: Nther_2645

Alternate gene names: 188587243

Gene position: 2842824-2844422 (Clockwise)

Preceding gene: 188587242

Following gene: 188587244

Centisome position: 89.8

GC content: 38.9

Gene sequence:

>1599_bases
ATGAAAAATTTAATTAGAGTATCATTGATTGGAATATTGGTTCTAACAGTTGGTTTAATGTTTACCGCCTGTGGAGGAGA
TGACAATGGTGATCCAGATAAAGACTCTAATGGAGAGAAAGCGTCAGATCAAGATCCTTTTATCATTGCAAGGGCTGGTG
ATTCTGACATCTTAGACCCTAATTATGCTACTTCTGAAGAGGATATGGATGTAGTTTTCCAGATTTTTGAAGGTTTATTA
AGGTTTGAAGATGACGATTTAAATGTTGGCCCAGGACTTGCTAATGAATGGGAAAGTTCCGAAGATGGTAAAACATGGAC
TTTTTACCTTCGCGATGATGTCACTTTTCACGACGGAAGTGAGTTTAATGCTGAAGCTGTAGAATTCAGTTTTATGAGAG
TTCTTGATGAAGATCACGAGTATCATGGTGTAATTGATGCTGGTTGGTCGTATTTAGACTATCTAATGGGAGACGTAATC
AATGACGTAAGAGCCGTTGATGATTATGTTGTTGAGTTTGAACTAGAGCAAAAGTTTGCACCTTTCGAAACATATATGGC
TTATTACTCTCAATTCATTGTCAGCCCTGAAGCTGTCAAAGAACATGGAGAAAACTTTGTAAATCATCCTACAGGTACTG
GTCCTTTCAAAATGGTCGAGTGGGATCGTGGCGATTATATTAGATTAGAAACTTACGATGATTACTGGGGTGAACTTCCC
GAAATTGACACCTTAATTTTTAAAGATATCCCAGAAGCTTCTACCAGGTTAATGGAACTGGAAACCGGATCTGTAGACGT
GGCTAAAAATGTTCCACCAGAACAAAGGGAAACCCTTGAAATGCTAGAAGAAGCTGAACTTGTGGAAATTCCCGGAGCTA
ACTTGTTTTACATGGCATTAAATCATGATGTAGAACCACTAAATGATGTTAAGGTAAGAAGAGCTATCAACCATGCCATT
GACGTAGATCAAATTATTGATGAAATTTATCAAGGTATGGCAACTAGAGCCATCAATGCATTACCTCCGACTGTATTCTC
CTTTGATGATACAGCTGGTCCTTATGAATACGATCCAGACAAAGCGGAAGAACTACTGGCAGAAGCTGGCTATGAAGATG
GCTTTGAAATCGAATTAAATACTTTCGTCTACTCAAGGACTTATGTAGACAACCCTGTACAAGTTGCTGAAGTAATAGCG
TCTGATTTGAGAGCTGTCGGAATAGATGCGCAAATAATCACTAATGAATGGGCACAACACTCAGACCTAACAGTTAATAT
GGAACACGACCTTGCATTAACAGGATGGTTTGACATCCCTTATCCCAGTAACTTTTTACGCACCTTGGTTCTAGAAGCCG
GATATAATGCTTATCAACCGGAAGAGCTAATGGATATGTCAAATGAAGCTTGGGCCACTTACGATGAAGAAGAACAAGAA
GAAATCTACCGAGAAATTCAGCAACGATTCCATGAAGATGTACCTTTAATTCCTATTGCTCACAGTAATTATACTGCAGC
AGTTAGAAACGATGTTGAAGGATTCGTACTTGATACAATCGGTATTATCAGTGCCCACGAAATTTCCAGAACTGAATAA

Upstream 100 bases:

>100_bases
GGGAGGGGGGTTAAAAAAAGACTGAGTACGTGTTATTAATTTGATTTAGTAAGATTAGTGAGAGATATAAAATTCAAATT
GTATTAGAGGAGGTTTGACA

Downstream 100 bases:

>100_bases
CTGATTTGATGGGGAGTGGCGACTCCCCATCAATTTTTAATTATTGAAGTTAATTTCTAACCTAAACAATCAAGAACTAA
TGACAACTTACTAAGGACAA

Product: 4-phytase

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKNLIRVSLIGILVLTVGLMFTACGGDDNGDPDKDSNGEKASDQDPFIIARAGDSDILDPNYATSEEDMDVVFQIFEGLL
RFEDDDLNVGPGLANEWESSEDGKTWTFYLRDDVTFHDGSEFNAEAVEFSFMRVLDEDHEYHGVIDAGWSYLDYLMGDVI
NDVRAVDDYVVEFELEQKFAPFETYMAYYSQFIVSPEAVKEHGENFVNHPTGTGPFKMVEWDRGDYIRLETYDDYWGELP
EIDTLIFKDIPEASTRLMELETGSVDVAKNVPPEQRETLEMLEEAELVEIPGANLFYMALNHDVEPLNDVKVRRAINHAI
DVDQIIDEIYQGMATRAINALPPTVFSFDDTAGPYEYDPDKAEELLAEAGYEDGFEIELNTFVYSRTYVDNPVQVAEVIA
SDLRAVGIDAQIITNEWAQHSDLTVNMEHDLALTGWFDIPYPSNFLRTLVLEAGYNAYQPEELMDMSNEAWATYDEEEQE
EIYREIQQRFHEDVPLIPIAHSNYTAAVRNDVEGFVLDTIGIISAHEISRTE

Sequences:

>Translated_532_residues
MKNLIRVSLIGILVLTVGLMFTACGGDDNGDPDKDSNGEKASDQDPFIIARAGDSDILDPNYATSEEDMDVVFQIFEGLL
RFEDDDLNVGPGLANEWESSEDGKTWTFYLRDDVTFHDGSEFNAEAVEFSFMRVLDEDHEYHGVIDAGWSYLDYLMGDVI
NDVRAVDDYVVEFELEQKFAPFETYMAYYSQFIVSPEAVKEHGENFVNHPTGTGPFKMVEWDRGDYIRLETYDDYWGELP
EIDTLIFKDIPEASTRLMELETGSVDVAKNVPPEQRETLEMLEEAELVEIPGANLFYMALNHDVEPLNDVKVRRAINHAI
DVDQIIDEIYQGMATRAINALPPTVFSFDDTAGPYEYDPDKAEELLAEAGYEDGFEIELNTFVYSRTYVDNPVQVAEVIA
SDLRAVGIDAQIITNEWAQHSDLTVNMEHDLALTGWFDIPYPSNFLRTLVLEAGYNAYQPEELMDMSNEAWATYDEEEQE
EIYREIQQRFHEDVPLIPIAHSNYTAAVRNDVEGFVLDTIGIISAHEISRTE
>Mature_532_residues
MKNLIRVSLIGILVLTVGLMFTACGGDDNGDPDKDSNGEKASDQDPFIIARAGDSDILDPNYATSEEDMDVVFQIFEGLL
RFEDDDLNVGPGLANEWESSEDGKTWTFYLRDDVTFHDGSEFNAEAVEFSFMRVLDEDHEYHGVIDAGWSYLDYLMGDVI
NDVRAVDDYVVEFELEQKFAPFETYMAYYSQFIVSPEAVKEHGENFVNHPTGTGPFKMVEWDRGDYIRLETYDDYWGELP
EIDTLIFKDIPEASTRLMELETGSVDVAKNVPPEQRETLEMLEEAELVEIPGANLFYMALNHDVEPLNDVKVRRAINHAI
DVDQIIDEIYQGMATRAINALPPTVFSFDDTAGPYEYDPDKAEELLAEAGYEDGFEIELNTFVYSRTYVDNPVQVAEVIA
SDLRAVGIDAQIITNEWAQHSDLTVNMEHDLALTGWFDIPYPSNFLRTLVLEAGYNAYQPEELMDMSNEAWATYDEEEQE
EIYREIQQRFHEDVPLIPIAHSNYTAAVRNDVEGFVLDTIGIISAHEISRTE

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=497, Percent_Identity=35.8148893360161, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1787551, Length=504, Percent_Identity=29.1666666666667, Blast_Score=208, Evalue=9e-55,
Organism=Escherichia coli, GI1787052, Length=488, Percent_Identity=27.4590163934426, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787762, Length=501, Percent_Identity=27.3453093812375, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1789887, Length=477, Percent_Identity=29.559748427673, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1787495, Length=542, Percent_Identity=23.9852398523985, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789397, Length=504, Percent_Identity=25.1984126984127, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI87081878, Length=474, Percent_Identity=22.3628691983122, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60455; Mature: 60455

Theoretical pI: Translated: 3.78; Mature: 3.78

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLIRVSLIGILVLTVGLMFTACGGDDNGDPDKDSNGEKASDQDPFIIARAGDSDILDP
CCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
NYATSEEDMDVVFQIFEGLLRFEDDDLNVGPGLANEWESSEDGKTWTFYLRDDVTFHDGS
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCC
EFNAEAVEFSFMRVLDEDHEYHGVIDAGWSYLDYLMGDVINDVRAVDDYVVEFELEQKFA
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCC
PFETYMAYYSQFIVSPEAVKEHGENFVNHPTGTGPFKMVEWDRGDYIRLETYDDYWGELP
HHHHHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCEEEEEECCCCEEEEEECHHHCCCCC
EIDTLIFKDIPEASTRLMELETGSVDVAKNVPPEQRETLEMLEEAELVEIPGANLFYMAL
CHHHHHHHCCCHHHHEEEEEECCCEEECCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEE
NHDVEPLNDVKVRRAINHAIDVDQIIDEIYQGMATRAINALPPTVFSFDDTAGPYEYDPD
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCH
KAEELLAEAGYEDGFEIELNTFVYSRTYVDNPVQVAEVIASDLRAVGIDAQIITNEWAQH
HHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHCCCC
SDLTVNMEHDLALTGWFDIPYPSNFLRTLVLEAGYNAYQPEELMDMSNEAWATYDEEEQE
CCEEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHH
EIYREIQQRFHEDVPLIPIAHSNYTAAVRNDVEGFVLDTIGIISAHEISRTE
HHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCEEEHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNLIRVSLIGILVLTVGLMFTACGGDDNGDPDKDSNGEKASDQDPFIIARAGDSDILDP
CCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
NYATSEEDMDVVFQIFEGLLRFEDDDLNVGPGLANEWESSEDGKTWTFYLRDDVTFHDGS
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCC
EFNAEAVEFSFMRVLDEDHEYHGVIDAGWSYLDYLMGDVINDVRAVDDYVVEFELEQKFA
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCC
PFETYMAYYSQFIVSPEAVKEHGENFVNHPTGTGPFKMVEWDRGDYIRLETYDDYWGELP
HHHHHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCEEEEEECCCCEEEEEECHHHCCCCC
EIDTLIFKDIPEASTRLMELETGSVDVAKNVPPEQRETLEMLEEAELVEIPGANLFYMAL
CHHHHHHHCCCHHHHEEEEEECCCEEECCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEE
NHDVEPLNDVKVRRAINHAIDVDQIIDEIYQGMATRAINALPPTVFSFDDTAGPYEYDPD
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCH
KAEELLAEAGYEDGFEIELNTFVYSRTYVDNPVQVAEVIASDLRAVGIDAQIITNEWAQH
HHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHCCCC
SDLTVNMEHDLALTGWFDIPYPSNFLRTLVLEAGYNAYQPEELMDMSNEAWATYDEEEQE
CCEEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHH
EIYREIQQRFHEDVPLIPIAHSNYTAAVRNDVEGFVLDTIGIISAHEISRTE
HHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCEEEHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]