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The map label for this gene is fdnH [H]

Identifier: 188587191

GI number: 188587191

Start: 2782812

End: 2783639

Strand: Direct

Name: fdnH [H]

Synonym: Nther_2592

Alternate gene names: 188587191

Gene position: 2782812-2783639 (Clockwise)

Preceding gene: 188587190

Following gene: 188587192

Centisome position: 87.91

GC content: 39.73

Gene sequence:

>828_bases
ATGACTAAAAGCATGTTGATAGATACATCTAAATGTACTGCTTGCAGAGCCTGTCAAATTGCTTGTAAACAATGGAATCA
ACTTCCTGCAGAAAAGACCAGTTTCACCGGTAGTTATGAAAACCCTACCAGTATCTCACCTGTAACTTGGACCAGAATTG
TCTTCCAGGAGCAGGAAAACCCCAATAATTCAGCTGGGATTAGCTGGTACTTCTCAAAACAAGGGTGCATGCACTGTACA
GATGCCGGTTGTTTGACAGTATGTCCAACTGGTGCAATTTATCGAACTGAATCGGGTACTGTTAATGTTGATTTTGACAG
ATGTATAGGTTGTAATTACTGTGCTGCCAATTGCCCCTTTAATGTTATAAGTTTCGACCGCAGGACTAATGTTCCTCCTA
AATGTACTTTCTGCCACGACAGAATGGTCAATGGATATCAACCTGCATGTGCCAGCGTCTGTCCATCCAATGCAATTATT
TACGGTGAACGACGTGATGTTATCAATACCGCCTTTAATAGAGTTAATCATTTAATAGAAAATGGCAATCCTAAAGCCAG
GGTTTATGGAGTAGATGAACTAGATGGCATGGGCATGATCTATGTATTAGAAAATGAGCCCGAATACTACGGTTTCCCGA
AAGAACCGGCTGTATCTACTTCGGCTAAAATTTGGGGCGCTATTTTCAGTCCTTTTAGATTATTAATTGCAATACTAGTT
AGTTTAGGGGTTCTAGCAAATAAATCAGAATATAACAAAGTAAATAGAGCTAAACAGCAAAAAGAAAAGCGCAATTCTGA
CAAAGAAGATCAGGGGGTGACAAAATGA

Upstream 100 bases:

>100_bases
GGAGCTGTGGCCAACGATGTTTCACCTTCAATCGGAGATGCAAACACCACCATCCCTGAATACAAAGCTTTTCTGTGTAA
TATAAGGAAGGTGAATAACT

Downstream 100 bases:

>100_bases
ATGTAAAATCCATAATTGGCGGAGCCTACCTTCCCCATTGTAAAGAATACACTGAAGAGCACAACATAAAAGACATCTCA
CTTCCAAAAGAAGTAGTAGT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: CO2; NADH2 [C]

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTKSMLIDTSKCTACRACQIACKQWNQLPAEKTSFTGSYENPTSISPVTWTRIVFQEQENPNNSAGISWYFSKQGCMHCT
DAGCLTVCPTGAIYRTESGTVNVDFDRCIGCNYCAANCPFNVISFDRRTNVPPKCTFCHDRMVNGYQPACASVCPSNAII
YGERRDVINTAFNRVNHLIENGNPKARVYGVDELDGMGMIYVLENEPEYYGFPKEPAVSTSAKIWGAIFSPFRLLIAILV
SLGVLANKSEYNKVNRAKQQKEKRNSDKEDQGVTK

Sequences:

>Translated_275_residues
MTKSMLIDTSKCTACRACQIACKQWNQLPAEKTSFTGSYENPTSISPVTWTRIVFQEQENPNNSAGISWYFSKQGCMHCT
DAGCLTVCPTGAIYRTESGTVNVDFDRCIGCNYCAANCPFNVISFDRRTNVPPKCTFCHDRMVNGYQPACASVCPSNAII
YGERRDVINTAFNRVNHLIENGNPKARVYGVDELDGMGMIYVLENEPEYYGFPKEPAVSTSAKIWGAIFSPFRLLIAILV
SLGVLANKSEYNKVNRAKQQKEKRNSDKEDQGVTK
>Mature_274_residues
TKSMLIDTSKCTACRACQIACKQWNQLPAEKTSFTGSYENPTSISPVTWTRIVFQEQENPNNSAGISWYFSKQGCMHCTD
AGCLTVCPTGAIYRTESGTVNVDFDRCIGCNYCAANCPFNVISFDRRTNVPPKCTFCHDRMVNGYQPACASVCPSNAIIY
GERRDVINTAFNRVNHLIENGNPKARVYGVDELDGMGMIYVLENEPEYYGFPKEPAVSTSAKIWGAIFSPFRLLIAILVS
LGVLANKSEYNKVNRAKQQKEKRNSDKEDQGVTK

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=246, Percent_Identity=38.2113821138211, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1790326, Length=272, Percent_Identity=34.5588235294118, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI226510944, Length=160, Percent_Identity=36.875, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1789370, Length=294, Percent_Identity=27.891156462585, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787122, Length=159, Percent_Identity=34.5911949685535, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787872, Length=159, Percent_Identity=34.5911949685535, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI2367345, Length=164, Percent_Identity=34.1463414634146, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787740, Length=80, Percent_Identity=38.75, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787478, Length=80, Percent_Identity=38.75, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082180, Length=160, Percent_Identity=31.875, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1789067, Length=180, Percent_Identity=30, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI2367245, Length=166, Percent_Identity=28.9156626506024, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787963, Length=164, Percent_Identity=24.390243902439, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: NA

Molecular weight: Translated: 30632; Mature: 30501

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSMLIDTSKCTACRACQIACKQWNQLPAEKTSFTGSYENPTSISPVTWTRIVFQEQEN
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCEEEEEEEECCCC
PNNSAGISWYFSKQGCMHCTDAGCLTVCPTGAIYRTESGTVNVDFDRCIGCNYCAANCPF
CCCCCCEEEEECCCCCCEECCCCEEEECCCCCEEECCCCEEEECHHHHCCCCCCCCCCCE
NVISFDRRTNVPPKCTFCHDRMVNGYQPACASVCPSNAIIYGERRDVINTAFNRVNHLIE
EEEEECCCCCCCCCEEEHHHHHCCCCCCHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHH
NGNPKARVYGVDELDGMGMIYVLENEPEYYGFPKEPAVSTSAKIWGAIFSPFRLLIAILV
CCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SLGVLANKSEYNKVNRAKQQKEKRNSDKEDQGVTK
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TKSMLIDTSKCTACRACQIACKQWNQLPAEKTSFTGSYENPTSISPVTWTRIVFQEQEN
CCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCEEEEEEEECCCC
PNNSAGISWYFSKQGCMHCTDAGCLTVCPTGAIYRTESGTVNVDFDRCIGCNYCAANCPF
CCCCCCEEEEECCCCCCEECCCCEEEECCCCCEEECCCCEEEECHHHHCCCCCCCCCCCE
NVISFDRRTNVPPKCTFCHDRMVNGYQPACASVCPSNAIIYGERRDVINTAFNRVNHLIE
EEEEECCCCCCCCCEEEHHHHHCCCCCCHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHH
NGNPKARVYGVDELDGMGMIYVLENEPEYYGFPKEPAVSTSAKIWGAIFSPFRLLIAILV
CCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SLGVLANKSEYNKVNRAKQQKEKRNSDKEDQGVTK
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD [C]

Specific reaction: formate + NAD = CO2 + NADH2 [C]

General reaction: Oxidoreductases; Acting on the aldehyde or oxo group of donors [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]