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The map label for this gene is carA [H]

Identifier: 188587053

GI number: 188587053

Start: 2621164

End: 2622363

Strand: Direct

Name: carA [H]

Synonym: Nther_2445

Alternate gene names: 188587053

Gene position: 2621164-2622363 (Clockwise)

Preceding gene: 188587052

Following gene: 188587054

Centisome position: 82.8

GC content: 42.25

Gene sequence:

>1200_bases
ATGCCGGATAAACTGATACTGGAAGATGGTACTGAATTTGAAGGAACGGTTGTTGGCCACAAAGTTACTGTTTCTGGGGA
AGTAGTATTTACTACCGGAATGACAGGTTATCAAGAAGTCCTGACTGACCCATCTTACTGCAATCAGATAGTTACCTTTA
CCTATCCCTTAATTGGAAATCAGGGAGTTAACAAACAGGATTTCGAATCGAAAAGGCCGTATTTAAAGGGAATTGTCACC
AAGGAAATTTGTGAACACCCGAGTAACTGGCAGTCAGAACTTCCATTAGCAGAGTATCTCAGAAATTGTAAAGTCACAGC
AATTACCGGAGTCGACACCAGAAAAGTGACAAAACACATTAGAGAAAAGGGTACCATGAAAGCTTGGATTATCTGTGAAG
ATAATTCCCATGAATCTGTTAACAACGATTCTAGTCATTATGAAATCCAAGAAACCAACCTGGTTGAACAGGTTTCTACC
CAAGAACCTTACGTACTGCCAGGAAACAACTACCGCATAGCCCTCCTCGATCTGGGGGTCAGGCATAGTCTACTAAAAGC
TCTTAACAACCTAGGAGCAACCGTCATAGTCCTCCCCCATGACACTCCACCAGAAGACATTCTCAGTTACGCTCCCCAGG
GAATTGTACTATCCAATGGGCCAGGAGACCCCCAAGAAATAATGGAACTGACAGATACCATCAACGAGGTCTTGAAACAC
CAAATTCCTCTCATGGGAGTCTGTCTAGGACATCAACTCCTAGGACTCACTCTTGGTTATGAGTCACTCAAAATGAAATT
TGGCCATAGAGGGATAAACCATCCCGTAAAAAACCTGGAAACAGATACGGTTAATATCACTTCTCAAAACCACGGTTATG
TCATATCAAGTGAAACAGTCCCAGCTAACACGGAAATCACTCATATCAACTTAAATGACCAGACAGTTGAAGGAATTAAA
CACAAACAATTACCTGTTTTCTCCGTCCAATTCCACCCCGAGGCGGGGTTATTAGACTTGGGAGACCAGCAAGAGAAAGC
TGAACTTTCCATAGATAAATTTATGAAACTGGTCCAAGACTATCAAAGTCGCAAATATTTAGGCCACAAGTATTTAGAAA
GTGACATCTCTAGTGCTAGCCAAATTAATCAAGGTGATGACGCTAATCACACTGACAAGGGGGAACAGTCTCATGCCTAA

Upstream 100 bases:

>100_bases
ATTTAATATCTGTGTTTATAGGAACAATAACTAAAGACTAAAAAATCAATGTGTCATTGAAGACTATCACAATTGAGGAT
TATCACAAGGGGGAATTTAC

Downstream 100 bases:

>100_bases
AAGAAATGATTTAAATAAAGTCTTAATAATCGGATCAGGCCCCATAATAATTGGCCAGGCTGTAGAGTTTGATTATGCCG
GAACCCAGGCCTGCAGTGTC

Product: carbamoyl-phosphate synthase, small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MPDKLILEDGTEFEGTVVGHKVTVSGEVVFTTGMTGYQEVLTDPSYCNQIVTFTYPLIGNQGVNKQDFESKRPYLKGIVT
KEICEHPSNWQSELPLAEYLRNCKVTAITGVDTRKVTKHIREKGTMKAWIICEDNSHESVNNDSSHYEIQETNLVEQVST
QEPYVLPGNNYRIALLDLGVRHSLLKALNNLGATVIVLPHDTPPEDILSYAPQGIVLSNGPGDPQEIMELTDTINEVLKH
QIPLMGVCLGHQLLGLTLGYESLKMKFGHRGINHPVKNLETDTVNITSQNHGYVISSETVPANTEITHINLNDQTVEGIK
HKQLPVFSVQFHPEAGLLDLGDQQEKAELSIDKFMKLVQDYQSRKYLGHKYLESDISSASQINQGDDANHTDKGEQSHA

Sequences:

>Translated_399_residues
MPDKLILEDGTEFEGTVVGHKVTVSGEVVFTTGMTGYQEVLTDPSYCNQIVTFTYPLIGNQGVNKQDFESKRPYLKGIVT
KEICEHPSNWQSELPLAEYLRNCKVTAITGVDTRKVTKHIREKGTMKAWIICEDNSHESVNNDSSHYEIQETNLVEQVST
QEPYVLPGNNYRIALLDLGVRHSLLKALNNLGATVIVLPHDTPPEDILSYAPQGIVLSNGPGDPQEIMELTDTINEVLKH
QIPLMGVCLGHQLLGLTLGYESLKMKFGHRGINHPVKNLETDTVNITSQNHGYVISSETVPANTEITHINLNDQTVEGIK
HKQLPVFSVQFHPEAGLLDLGDQQEKAELSIDKFMKLVQDYQSRKYLGHKYLESDISSASQINQGDDANHTDKGEQSHA
>Mature_398_residues
PDKLILEDGTEFEGTVVGHKVTVSGEVVFTTGMTGYQEVLTDPSYCNQIVTFTYPLIGNQGVNKQDFESKRPYLKGIVTK
EICEHPSNWQSELPLAEYLRNCKVTAITGVDTRKVTKHIREKGTMKAWIICEDNSHESVNNDSSHYEIQETNLVEQVSTQ
EPYVLPGNNYRIALLDLGVRHSLLKALNNLGATVIVLPHDTPPEDILSYAPQGIVLSNGPGDPQEIMELTDTINEVLKHQ
IPLMGVCLGHQLLGLTLGYESLKMKFGHRGINHPVKNLETDTVNITSQNHGYVISSETVPANTEITHINLNDQTVEGIKH
KQLPVFSVQFHPEAGLLDLGDQQEKAELSIDKFMKLVQDYQSRKYLGHKYLESDISSASQINQGDDANHTDKGEQSHA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=370, Percent_Identity=38.1081081081081, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI169790915, Length=370, Percent_Identity=34.8648648648649, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI21361331, Length=370, Percent_Identity=34.8648648648649, Blast_Score=208, Evalue=6e-54,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=40.053050397878, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1789760, Length=168, Percent_Identity=29.1666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=383, Percent_Identity=38.6422976501305, Blast_Score=262, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324878, Length=379, Percent_Identity=39.5778364116095, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6322331, Length=408, Percent_Identity=34.5588235294118, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI45555749, Length=383, Percent_Identity=38.6422976501305, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24642586, Length=383, Percent_Identity=38.9033942558747, Blast_Score=224, Evalue=8e-59,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 44421; Mature: 44289

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDKLILEDGTEFEGTVVGHKVTVSGEVVFTTGMTGYQEVLTDPSYCNQIVTFTYPLIGN
CCCCEEECCCCCCCCEEEEEEEEECCEEEEEECCCHHHHHHCCHHHHCCEEEEEEEECCC
QGVNKQDFESKRPYLKGIVTKEICEHPSNWQSELPLAEYLRNCKVTAITGVDTRKVTKHI
CCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEEECCCHHHHHHHH
REKGTMKAWIICEDNSHESVNNDSSHYEIQETNLVEQVSTQEPYVLPGNNYRIALLDLGV
HHCCCEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCCCCEEECCCCCEEEEEECCH
RHSLLKALNNLGATVIVLPHDTPPEDILSYAPQGIVLSNGPGDPQEIMELTDTINEVLKH
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
QIPLMGVCLGHQLLGLTLGYESLKMKFGHRGINHPVKNLETDTVNITSQNHGYVISSETV
CCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHCCCCCEEEEEECCCCEEEECCCC
PANTEITHINLNDQTVEGIKHKQLPVFSVQFHPEAGLLDLGDQQEKAELSIDKFMKLVQD
CCCCEEEEEECCCHHHHCCHHCCCCEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHH
YQSRKYLGHKYLESDISSASQINQGDDANHTDKGEQSHA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDKLILEDGTEFEGTVVGHKVTVSGEVVFTTGMTGYQEVLTDPSYCNQIVTFTYPLIGN
CCCEEECCCCCCCCEEEEEEEEECCEEEEEECCCHHHHHHCCHHHHCCEEEEEEEECCC
QGVNKQDFESKRPYLKGIVTKEICEHPSNWQSELPLAEYLRNCKVTAITGVDTRKVTKHI
CCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEEECCCHHHHHHHH
REKGTMKAWIICEDNSHESVNNDSSHYEIQETNLVEQVSTQEPYVLPGNNYRIALLDLGV
HHCCCEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCCCCEEECCCCCEEEEEECCH
RHSLLKALNNLGATVIVLPHDTPPEDILSYAPQGIVLSNGPGDPQEIMELTDTINEVLKH
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
QIPLMGVCLGHQLLGLTLGYESLKMKFGHRGINHPVKNLETDTVNITSQNHGYVISSETV
CCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHCCCCCEEEEEECCCCEEEECCCC
PANTEITHINLNDQTVEGIKHKQLPVFSVQFHPEAGLLDLGDQQEKAELSIDKFMKLVQD
CCCCEEEEEECCCHHHHCCHHCCCCEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHH
YQSRKYLGHKYLESDISSASQINQGDDANHTDKGEQSHA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]