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The map label for this gene is rihA [H]

Identifier: 188586916

GI number: 188586916

Start: 2451567

End: 2452559

Strand: Reverse

Name: rihA [H]

Synonym: Nther_2307

Alternate gene names: 188586916

Gene position: 2452559-2451567 (Counterclockwise)

Preceding gene: 188586917

Following gene: 188586915

Centisome position: 77.48

GC content: 41.99

Gene sequence:

>993_bases
ATGAAAGCAAAAAACTATATTTCGGGAAACAGAAAACCTGTAATTTTAGACACTGATCCAGGTATGGACGATGCCATTGC
TCTGTTTTTTCTCATGGGAAGACAGGATATTTTAGAACCTGTTGCCATAACAACGGTTTTTGGTAATGTATCAATTGACA
AAACCACTCACAATGCTCAAAAGCTACTGAAGTTTTTAGATGCGGAAGAAGTTCCTGTTTATAGGGGGGCTAGTTCCCCT
TTAATCAGGGATAGAGTTTCAGGTGAAATGATCCATGGTGAGTCGGGACTGGGGGAAGTGGATTTTCCGGAACCGGAAAA
AAGTAGCGATGCAACCAGTACTCCCGCGGCTGTAGCCATGGCAAAAGAAATTATGGCTCGACCCGGTGAGATAACGGTTA
TCACTGTAGGACCTATGACTAATTTAGCTCTGGCCCTTAAGCTATACCCTGAAATAAAAGAACAGATAGCAGAAGTAATA
GTTATGGGAGGAGCCATGGGGCAGGGAAATCGGACAGCTTCAGCCGAATATAATGTTTTTGCTGACCCCCATAGTGCACA
AATTGTGTTTGAAAGCGGATTATCAGTTGCCATGATAGGTTTAGATGTGACAGAACAGAGTAAACCAGGTCAGAAAGAGC
GCCAGTTACTACAAAAGTTGGATCATCCTGTTGCTACGAAAACTTTAGAATTTATGGACTTTTTCAAAAGACAGATGAAA
ACAGATTACGATATGGCTTTTCATGATCCTTGTGCCATCGCTTATGCCATTAATCCTGATATCTTTACGACTCGGTCCAT
GGACGTCAGAATAGAACTTACGGGAACATATACCCTAGGAAGAACTGTTTGTTCCCGGGGTCAAGAGGAAGTTAAAGGAG
TCATGGGAGATCCCGATTCCAGAAATGTTAAGGTCGGATTAGGTGTTGACCGGGAAAAGTTTTTTGAAGAGTTGTTTACA
TCTTTCCGAAAACTGGCCGATACAATGAATTAA

Upstream 100 bases:

>100_bases
CCACACATAGCAAAGTCATATAGTGAGTTCATATAGAAAAATGCTAAATCTACATTGCCAGCAGAGCAGAACAGAACAGA
AATGGAGGTAATGAAAGAAA

Downstream 100 bases:

>100_bases
TTACATGGATTAACTACATGTTACTAGAATTTTGTGGTTATACTAGAGGATAAGACATACTTTATCAGATATAAGATATA
GGAGTGTACTTATGATAGAT

Product: Inosine/uridine-preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKAKNYISGNRKPVILDTDPGMDDAIALFFLMGRQDILEPVAITTVFGNVSIDKTTHNAQKLLKFLDAEEVPVYRGASSP
LIRDRVSGEMIHGESGLGEVDFPEPEKSSDATSTPAAVAMAKEIMARPGEITVITVGPMTNLALALKLYPEIKEQIAEVI
VMGGAMGQGNRTASAEYNVFADPHSAQIVFESGLSVAMIGLDVTEQSKPGQKERQLLQKLDHPVATKTLEFMDFFKRQMK
TDYDMAFHDPCAIAYAINPDIFTTRSMDVRIELTGTYTLGRTVCSRGQEEVKGVMGDPDSRNVKVGLGVDREKFFEELFT
SFRKLADTMN

Sequences:

>Translated_330_residues
MKAKNYISGNRKPVILDTDPGMDDAIALFFLMGRQDILEPVAITTVFGNVSIDKTTHNAQKLLKFLDAEEVPVYRGASSP
LIRDRVSGEMIHGESGLGEVDFPEPEKSSDATSTPAAVAMAKEIMARPGEITVITVGPMTNLALALKLYPEIKEQIAEVI
VMGGAMGQGNRTASAEYNVFADPHSAQIVFESGLSVAMIGLDVTEQSKPGQKERQLLQKLDHPVATKTLEFMDFFKRQMK
TDYDMAFHDPCAIAYAINPDIFTTRSMDVRIELTGTYTLGRTVCSRGQEEVKGVMGDPDSRNVKVGLGVDREKFFEELFT
SFRKLADTMN
>Mature_330_residues
MKAKNYISGNRKPVILDTDPGMDDAIALFFLMGRQDILEPVAITTVFGNVSIDKTTHNAQKLLKFLDAEEVPVYRGASSP
LIRDRVSGEMIHGESGLGEVDFPEPEKSSDATSTPAAVAMAKEIMARPGEITVITVGPMTNLALALKLYPEIKEQIAEVI
VMGGAMGQGNRTASAEYNVFADPHSAQIVFESGLSVAMIGLDVTEQSKPGQKERQLLQKLDHPVATKTLEFMDFFKRQMK
TDYDMAFHDPCAIAYAINPDIFTTRSMDVRIELTGTYTLGRTVCSRGQEEVKGVMGDPDSRNVKVGLGVDREKFFEELFT
SFRKLADTMN

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=312, Percent_Identity=38.4615384615385, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1788486, Length=320, Percent_Identity=36.5625, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1786213, Length=304, Percent_Identity=38.4868421052632, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32565474, Length=293, Percent_Identity=30.7167235494881, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17565698, Length=293, Percent_Identity=27.6450511945392, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=346, Percent_Identity=28.9017341040462, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24641839, Length=295, Percent_Identity=30.5084745762712, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583915, Length=329, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641837, Length=336, Percent_Identity=27.9761904761905, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 36283; Mature: 36283

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKNYISGNRKPVILDTDPGMDDAIALFFLMGRQDILEPVAITTVFGNVSIDKTTHNAQ
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHCCEEEEEEECCEEECCCHHHHH
KLLKFLDAEEVPVYRGASSPLIRDRVSGEMIHGESGLGEVDFPEPEKSSDATSTPAAVAM
HHHHHHCCCCCCEECCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
AKEIMARPGEITVITVGPMTNLALALKLYPEIKEQIAEVIVMGGAMGQGNRTASAEYNVF
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
ADPHSAQIVFESGLSVAMIGLDVTEQSKPGQKERQLLQKLDHPVATKTLEFMDFFKRQMK
ECCCCCEEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
TDYDMAFHDPCAIAYAINPDIFTTRSMDVRIELTGTYTLGRTVCSRGQEEVKGVMGDPDS
CCCCEEECCCEEEEEEECCCEEEECCEEEEEEEEECHHHHHHHHHCCHHHHHCCCCCCCC
RNVKVGLGVDREKFFEELFTSFRKLADTMN
CCEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAKNYISGNRKPVILDTDPGMDDAIALFFLMGRQDILEPVAITTVFGNVSIDKTTHNAQ
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHCCEEEEEEECCEEECCCHHHHH
KLLKFLDAEEVPVYRGASSPLIRDRVSGEMIHGESGLGEVDFPEPEKSSDATSTPAAVAM
HHHHHHCCCCCCEECCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
AKEIMARPGEITVITVGPMTNLALALKLYPEIKEQIAEVIVMGGAMGQGNRTASAEYNVF
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
ADPHSAQIVFESGLSVAMIGLDVTEQSKPGQKERQLLQKLDHPVATKTLEFMDFFKRQMK
ECCCCCEEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
TDYDMAFHDPCAIAYAINPDIFTTRSMDVRIELTGTYTLGRTVCSRGQEEVKGVMGDPDS
CCCCEEECCCEEEEEEECCCEEEECCEEEEEEEEECHHHHHHHHHCCHHHHHCCCCCCCC
RNVKVGLGVDREKFFEELFTSFRKLADTMN
CCEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA