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The map label for this gene is ackA

Identifier: 188586912

GI number: 188586912

Start: 2446565

End: 2447761

Strand: Direct

Name: ackA

Synonym: Nther_2303

Alternate gene names: 188586912

Gene position: 2446565-2447761 (Clockwise)

Preceding gene: 188586911

Following gene: 188586919

Centisome position: 77.29

GC content: 40.94

Gene sequence:

>1197_bases
ATGAAAATTTTGGTGATCAACTGCGGCAGTTCGTCGCTCAAATATCAAGTTTTCAACATGGAAAATGAGGACGTACTAGC
CAAAGGCGCTATTGAAAGAATTGGTATTGAAGGGTCCTTTTTGAAGCACGAACCCGCAGGTGGTGAAGAAGTTAAAATTG
AGAAAGATGTCACAGATCACAAGGCTGCTTTCAAAATGGTAGTTGACGCAATTACTGATGAGGGACACGGTGTATTAAAA
GATCTAAAAGAAATAGAAGCTGTAGGTCATCGCGTTGTTCACGGTGGAGAAGAATTCTCCGGTTCTGTAATTATCGACGA
TGATGTTATGAATGCCCTGGAAAGCTGCATTAACTTGGCTCCACTTCACAATCCTCCCAACATATATGGTATCGAAGCTT
CTAAAGCAAATATGCCAGGTGTTCCTCAAGTTGGAGTATTTGACACAGCATTTCACCAGACTATGCCTAAAAAGGCATAT
TTATACGGAATCCCATACGAATACTATGAAAAACACAGTGTACGCAGATACGGTTTCCATGGAACTTCCCATAAGTATGT
AGCCAGCAGAGCTGCAGACATGTTAGGGAAACCTTTGGAAGATTTAAAATTAATCACTTGTCATTTGGGTAACGGAGCGA
GTGTTGCTGCAATTAATAAGGGAGAATCTGTAGACACCAGTATGGGGCTAACTCCCCTTGAAGGACTTGTGATGGGAACT
CGATCCGGAAGCATGGATCCATCCATTATTAAATTCTTAATGGACAAAGAAGGTCTTTCCATTGACGAAGTCGATAACAT
CTTGAATAAAAAATCAGGTGTTCTGGGTCTATCTGGTTTAAGCAATGACTTCAGAGACTTGGAAGAAGCTGCTGCAGAAG
GTAATGAAAGAGCTGAAAACACTTTAGAGGTGTTCTATTATACTGTATCAAAATATATCGGTTCTTATCTAGCTGCTTTA
AATGGAGCAGATGCTATTATCTTTACTGCTGGTCTAGGTGAAAACTCACCTTCCGTAAGAGAAGCTGTGACAGAAAACCT
TTCATATGCTGGCATTAAAGTAGACAAGGACAAAAACCAGGTACGCGGTAAAGAAACTGATATCAGTACTGACGACGCAT
CCACTAGAGTCCTTGTTGTACCAACTAACGAAGAGCTTATGATCGCAAGGGAAGCTAAGGGACTACTAGGAAAGTAA

Upstream 100 bases:

>100_bases
AAAAAAATACCTGTAGTAATTCTTGTGTTTTATAGAAAAAATTAATATAATAAAGTGTGGTAGATTTAGTTATACAAATA
ACATATAAGGGGGATTATTA

Downstream 100 bases:

>100_bases
GAGACTACTTAACTTTGATTTATAACGATTAAAACAACTAACCCCTAGACCTGCTTAAAAGGAAGGCACGGTCTAGGGGT
ATTTTTATAGACAAATTAAT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKILVINCGSSSLKYQVFNMENEDVLAKGAIERIGIEGSFLKHEPAGGEEVKIEKDVTDHKAAFKMVVDAITDEGHGVLK
DLKEIEAVGHRVVHGGEEFSGSVIIDDDVMNALESCINLAPLHNPPNIYGIEASKANMPGVPQVGVFDTAFHQTMPKKAY
LYGIPYEYYEKHSVRRYGFHGTSHKYVASRAADMLGKPLEDLKLITCHLGNGASVAAINKGESVDTSMGLTPLEGLVMGT
RSGSMDPSIIKFLMDKEGLSIDEVDNILNKKSGVLGLSGLSNDFRDLEEAAAEGNERAENTLEVFYYTVSKYIGSYLAAL
NGADAIIFTAGLGENSPSVREAVTENLSYAGIKVDKDKNQVRGKETDISTDDASTRVLVVPTNEELMIAREAKGLLGK

Sequences:

>Translated_398_residues
MKILVINCGSSSLKYQVFNMENEDVLAKGAIERIGIEGSFLKHEPAGGEEVKIEKDVTDHKAAFKMVVDAITDEGHGVLK
DLKEIEAVGHRVVHGGEEFSGSVIIDDDVMNALESCINLAPLHNPPNIYGIEASKANMPGVPQVGVFDTAFHQTMPKKAY
LYGIPYEYYEKHSVRRYGFHGTSHKYVASRAADMLGKPLEDLKLITCHLGNGASVAAINKGESVDTSMGLTPLEGLVMGT
RSGSMDPSIIKFLMDKEGLSIDEVDNILNKKSGVLGLSGLSNDFRDLEEAAAEGNERAENTLEVFYYTVSKYIGSYLAAL
NGADAIIFTAGLGENSPSVREAVTENLSYAGIKVDKDKNQVRGKETDISTDDASTRVLVVPTNEELMIAREAKGLLGK
>Mature_398_residues
MKILVINCGSSSLKYQVFNMENEDVLAKGAIERIGIEGSFLKHEPAGGEEVKIEKDVTDHKAAFKMVVDAITDEGHGVLK
DLKEIEAVGHRVVHGGEEFSGSVIIDDDVMNALESCINLAPLHNPPNIYGIEASKANMPGVPQVGVFDTAFHQTMPKKAY
LYGIPYEYYEKHSVRRYGFHGTSHKYVASRAADMLGKPLEDLKLITCHLGNGASVAAINKGESVDTSMGLTPLEGLVMGT
RSGSMDPSIIKFLMDKEGLSIDEVDNILNKKSGVLGLSGLSNDFRDLEEAAAEGNERAENTLEVFYYTVSKYIGSYLAAL
NGADAIIFTAGLGENSPSVREAVTENLSYAGIKVDKDKNQVRGKETDISTDDASTRVLVVPTNEELMIAREAKGLLGK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=43.5768261964735, Blast_Score=334, Evalue=6e-93,
Organism=Escherichia coli, GI145693184, Length=392, Percent_Identity=43.8775510204082, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_NATTJ (B2A8I5)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918457.1
- ProteinModelPortal:   B2A8I5
- SMR:   B2A8I5
- GeneID:   6314642
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2303
- HOGENOM:   HBG285863
- OMA:   ENSVEVR
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43048; Mature: 43048

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVINCGSSSLKYQVFNMENEDVLAKGAIERIGIEGSFLKHEPAGGEEVKIEKDVTDH
CEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEECCCCCCCCEEEEECCCCHH
KAAFKMVVDAITDEGHGVLKDLKEIEAVGHRVVHGGEEFSGSVIIDDDVMNALESCINLA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCEEECHHHHHHHHHHHHHC
PLHNPPNIYGIEASKANMPGVPQVGVFDTAFHQTMPKKAYLYGIPYEYYEKHSVRRYGFH
CCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCHHHHHCCCCCEECCC
GTSHKYVASRAADMLGKPLEDLKLITCHLGNGASVAAINKGESVDTSMGLTPLEGLVMGT
CCCCHHHHHHHHHHHCCCHHHCEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RSGSMDPSIIKFLMDKEGLSIDEVDNILNKKSGVLGLSGLSNDFRDLEEAAAEGNERAEN
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHH
TLEVFYYTVSKYIGSYLAALNGADAIIFTAGLGENSPSVREAVTENLSYAGIKVDKDKNQ
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCHHH
VRGKETDISTDDASTRVLVVPTNEELMIAREAKGLLGK
CCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCC
>Mature Secondary Structure
MKILVINCGSSSLKYQVFNMENEDVLAKGAIERIGIEGSFLKHEPAGGEEVKIEKDVTDH
CEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEECCCCCCCCEEEEECCCCHH
KAAFKMVVDAITDEGHGVLKDLKEIEAVGHRVVHGGEEFSGSVIIDDDVMNALESCINLA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCEEECHHHHHHHHHHHHHC
PLHNPPNIYGIEASKANMPGVPQVGVFDTAFHQTMPKKAYLYGIPYEYYEKHSVRRYGFH
CCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCHHHHHCCCCCEECCC
GTSHKYVASRAADMLGKPLEDLKLITCHLGNGASVAAINKGESVDTSMGLTPLEGLVMGT
CCCCHHHHHHHHHHHCCCHHHCEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RSGSMDPSIIKFLMDKEGLSIDEVDNILNKKSGVLGLSGLSNDFRDLEEAAAEGNERAEN
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHH
TLEVFYYTVSKYIGSYLAALNGADAIIFTAGLGENSPSVREAVTENLSYAGIKVDKDKNQ
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCHHH
VRGKETDISTDDASTRVLVVPTNEELMIAREAKGLLGK
CCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA