Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 188586825

GI number: 188586825

Start: 2352427

End: 2353461

Strand: Reverse

Name: prfB [H]

Synonym: Nther_2216

Alternate gene names: 188586825

Gene position: 2353461-2352427 (Counterclockwise)

Preceding gene: 188586826

Following gene: 188586824

Centisome position: 74.35

GC content: 43.96

Gene sequence:

>1035_bases
ATGGAGAAGCGCTTAAAAGAACTTGAAAAGCAAATGACTGAACCCGGCTTTTGGGATGATCAAGACCGGGCCAAAAAGGT
GACCCAGGAACGACAGGAACTTAAAGAACATTTAGATGAGATAAAGGAACTGGATGAACTCAAGGATGAAGTTTCTGTCA
TGATTGAGTTGTCTGAAGAAGACGAAACAGATGGAACTAATATGAAAGAAATAAAACAGGCCTTGTTTCAATTTGAAGAA
CAGTTATCAGAGATCAGGCTAAAAAATATGCTGAAAGACCGCTATGATGAAAATAATGCTATCATTAGCTTACATGCCGG
TGCCGGTGGGACTGAAAGCCAGGATTGGGTGGAAATGTTGCACAGAATGTACATCCGCTGGATGGAGCACCGGGGCTTTA
ATTACGAGACCCTGGACTTTTTACCGGGAGATGAAGCCGGTGTAAAATCAGTAACCATGATGGTGAAAGGAAAGTATGCC
TTTGGTTACTTAAAATCAGAAGCCGGAGTACACAGATTGGTTAGGGTGTCACCCTTTGATTCTTCCGGTAGAAGACATAC
TTCTTTCGCATCGATGGATGTGGTACCGGAAATTGATGATGATATAGAAATTGACATCAAGGATGATGACCTAAAGATTG
ACACATACCGGGCCAGCGGAGCCGGAGGACAGCATGTTAATACCACGGATTCCGCCGTTCGGATCACCCACCATCCTACA
GGAATTGTGGTTCAATGTCAGAATGAAAGGTCTCAGCATAAAAACCGGGAAGTTGCCATGAAGATTTTGAAGGCAAAGCT
GGCAGCCATTGAAGAGGAAAAACGCCGACAGGAACAAGAAGCCATGCGGGGGGATAAAAAAGAGATCGCCTGGGGTAGCC
AGATTAGATCTTATGTTTTTCACCCCTATACCATGGTAAAAGATCATAGAACCAACCTGGAAGAAGGCAATGCCGATGCC
GTCCTGGATGGAAAGTTGGATCATTTTATCCAGGAATATCTGAAGCAAAGAATGAATGACAGGATGTCCCAGTAA

Upstream 100 bases:

>100_bases
AGTGACAAAAGAATTCAAAACATGGGTGAATTAAAGACCAGATTTCAAGAGCTAACTGAACAATTAAACGAAATGAGGGC
TTCACTTTGACCCGGATGGG

Downstream 100 bases:

>100_bases
CCGATAACGAGCTCGACACAAAACAAGCCTTCACATCAGGAAAGGAGGAGTAGCTATGAAAAAGATAGTTTTTAAAGAAA
GTCACAAAAAGAAGTTAAAG

Product: peptide chain release factor 2 (bRF-2)

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MEKRLKELEKQMTEPGFWDDQDRAKKVTQERQELKEHLDEIKELDELKDEVSVMIELSEEDETDGTNMKEIKQALFQFEE
QLSEIRLKNMLKDRYDENNAIISLHAGAGGTESQDWVEMLHRMYIRWMEHRGFNYETLDFLPGDEAGVKSVTMMVKGKYA
FGYLKSEAGVHRLVRVSPFDSSGRRHTSFASMDVVPEIDDDIEIDIKDDDLKIDTYRASGAGGQHVNTTDSAVRITHHPT
GIVVQCQNERSQHKNREVAMKILKAKLAAIEEEKRRQEQEAMRGDKKEIAWGSQIRSYVFHPYTMVKDHRTNLEEGNADA
VLDGKLDHFIQEYLKQRMNDRMSQ

Sequences:

>Translated_344_residues
MEKRLKELEKQMTEPGFWDDQDRAKKVTQERQELKEHLDEIKELDELKDEVSVMIELSEEDETDGTNMKEIKQALFQFEE
QLSEIRLKNMLKDRYDENNAIISLHAGAGGTESQDWVEMLHRMYIRWMEHRGFNYETLDFLPGDEAGVKSVTMMVKGKYA
FGYLKSEAGVHRLVRVSPFDSSGRRHTSFASMDVVPEIDDDIEIDIKDDDLKIDTYRASGAGGQHVNTTDSAVRITHHPT
GIVVQCQNERSQHKNREVAMKILKAKLAAIEEEKRRQEQEAMRGDKKEIAWGSQIRSYVFHPYTMVKDHRTNLEEGNADA
VLDGKLDHFIQEYLKQRMNDRMSQ
>Mature_344_residues
MEKRLKELEKQMTEPGFWDDQDRAKKVTQERQELKEHLDEIKELDELKDEVSVMIELSEEDETDGTNMKEIKQALFQFEE
QLSEIRLKNMLKDRYDENNAIISLHAGAGGTESQDWVEMLHRMYIRWMEHRGFNYETLDFLPGDEAGVKSVTMMVKGKYA
FGYLKSEAGVHRLVRVSPFDSSGRRHTSFASMDVVPEIDDDIEIDIKDDDLKIDTYRASGAGGQHVNTTDSAVRITHHPT
GIVVQCQNERSQHKNREVAMKILKAKLAAIEEEKRRQEQEAMRGDKKEIAWGSQIRSYVFHPYTMVKDHRTNLEEGNADA
VLDGKLDHFIQEYLKQRMNDRMSQ

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=305, Percent_Identity=37.7049180327869, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI34577120, Length=254, Percent_Identity=39.3700787401575, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=37.6811594202899, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI2367172, Length=333, Percent_Identity=49.8498498498498, Blast_Score=341, Evalue=3e-95,
Organism=Escherichia coli, GI1787462, Length=258, Percent_Identity=43.4108527131783, Blast_Score=204, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=339, Percent_Identity=31.2684365781711, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6321295, Length=364, Percent_Identity=31.3186813186813, Blast_Score=149, Evalue=6e-37,
Organism=Drosophila melanogaster, GI19921226, Length=308, Percent_Identity=39.9350649350649, Blast_Score=181, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39997; Mature: 39997

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRLKELEKQMTEPGFWDDQDRAKKVTQERQELKEHLDEIKELDELKDEVSVMIELSEE
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
DETDGTNMKEIKQALFQFEEQLSEIRLKNMLKDRYDENNAIISLHAGAGGTESQDWVEML
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHH
HRMYIRWMEHRGFNYETLDFLPGDEAGVKSVTMMVKGKYAFGYLKSEAGVHRLVRVSPFD
HHHHHHHHHHCCCCCCEECCCCCCCCCHHHEEHEEECCHHHHHHHHHHCHHHEEEECCCC
SSGRRHTSFASMDVVPEIDDDIEIDIKDDDLKIDTYRASGAGGQHVNTTDSAVRITHHPT
CCCCCCCCHHCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCC
GIVVQCQNERSQHKNREVAMKILKAKLAAIEEEKRRQEQEAMRGDKKEIAWGSQIRSYVF
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
HPYTMVKDHRTNLEEGNADAVLDGKLDHFIQEYLKQRMNDRMSQ
CCHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKRLKELEKQMTEPGFWDDQDRAKKVTQERQELKEHLDEIKELDELKDEVSVMIELSEE
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
DETDGTNMKEIKQALFQFEEQLSEIRLKNMLKDRYDENNAIISLHAGAGGTESQDWVEML
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHH
HRMYIRWMEHRGFNYETLDFLPGDEAGVKSVTMMVKGKYAFGYLKSEAGVHRLVRVSPFD
HHHHHHHHHHCCCCCCEECCCCCCCCCHHHEEHEEECCHHHHHHHHHHCHHHEEEECCCC
SSGRRHTSFASMDVVPEIDDDIEIDIKDDDLKIDTYRASGAGGQHVNTTDSAVRITHHPT
CCCCCCCCHHCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCC
GIVVQCQNERSQHKNREVAMKILKAKLAAIEEEKRRQEQEAMRGDKKEIAWGSQIRSYVF
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
HPYTMVKDHRTNLEEGNADAVLDGKLDHFIQEYLKQRMNDRMSQ
CCHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]