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The map label for this gene is appB [H]

Identifier: 188586806

GI number: 188586806

Start: 2333160

End: 2334137

Strand: Direct

Name: appB [H]

Synonym: Nther_2197

Alternate gene names: 188586806

Gene position: 2333160-2334137 (Clockwise)

Preceding gene: 188586799

Following gene: 188586807

Centisome position: 73.7

GC content: 42.02

Gene sequence:

>978_bases
TTGGGTACACAGCAATTTATACTCAGGCGATTAATTCAAATGATTATCAGCATTTTTGTAGTGGCAACTGTAATCTTTTT
CATGTTCAGATTGCTTCCCGGTGACCCAACTACGGCAATGATAGAACCAGGTTGGTCCCAGGAAGCAACCCAAATGTTAA
TTGAGCGCTTTGGGCTGGACAGGCCACTACACGAACAGTATTTTCATTATTTAGTAAACCTGTTTCAATTGGATTTTGGG
TATTCATTCCATTATTCCTCACCAGTAAGTTCTGTTATTGGCGCAAAAATTGGAAACACCTTGATCCTAATGCTATCGGC
TATGATTGTAGCTTATGTCATTGGAATCCTTGGTGGAGTACTCATGGCCTGGTATCGAGGGTCTAAACTAGAAACATTCG
GTATCAGTGCAGCTTTAATTTTCAGGGCCTTACCACCCTTTTTCGTTGGAATGCTTTTTATTATGTTATTTAGTTTTCAT
TTAGGATGGCTTCCCCACTCGGGAATGAGAACAGCTGGCTACGTAGCCGAAGGAGCTTTAGACAGATTTTTTAACTTGGA
CTTTTTACGTCACTTAATATTACCTATGACAGTCTCGGCTCTATATTTTCTTGCCCAACCCCTATTGATAATGAGAAATA
CCATGATGGAAGTGATGGGAGAAGATTTTGTGGAAATGGCCGACGCTAAGGGATTAAAAAAAGTTAGGATTATGTACGTC
CATGCCGCGCGAAATGCGTTACTACCTGTTGTAACTCAGGGAGCATTATTCTTGGGAATGGCCGTAGGAGGTCAGGTGTT
AATCGAATACGTATTTGGCTGGCCCGGTCTGGGAGAAGAAATTGTCCTGGCAGCTCAAAGGCACGACTATCCAGTAGCCC
AGGCATCCTTTTTAATGATGGCCAGTTTGGTTATGCTTATGAACTTCCTAGCTGATCTGTTGTACGGCTACCTAGATCCA
AGGGTTGTTTACAATTAA

Upstream 100 bases:

>100_bases
ATTAACGTTATAAAGTGTTAGTGAGTTAAACTGTTACTTGACTAAATGATTAAGGGAAGGGACTAGGCTAAATCTAAAAT
CAATAAAGGTGGTGAATTTC

Downstream 100 bases:

>100_bases
AACATCAAATAAAAACAGATATTTTTAAAAGCGAGGTGACAGACAATTGGATACGCTGTTAAAGTTTTTCAGATCAGCAT
ATGACATACTTTCTAAAGAC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MGTQQFILRRLIQMIISIFVVATVIFFMFRLLPGDPTTAMIEPGWSQEATQMLIERFGLDRPLHEQYFHYLVNLFQLDFG
YSFHYSSPVSSVIGAKIGNTLILMLSAMIVAYVIGILGGVLMAWYRGSKLETFGISAALIFRALPPFFVGMLFIMLFSFH
LGWLPHSGMRTAGYVAEGALDRFFNLDFLRHLILPMTVSALYFLAQPLLIMRNTMMEVMGEDFVEMADAKGLKKVRIMYV
HAARNALLPVVTQGALFLGMAVGGQVLIEYVFGWPGLGEEIVLAAQRHDYPVAQASFLMMASLVMLMNFLADLLYGYLDP
RVVYN

Sequences:

>Translated_325_residues
MGTQQFILRRLIQMIISIFVVATVIFFMFRLLPGDPTTAMIEPGWSQEATQMLIERFGLDRPLHEQYFHYLVNLFQLDFG
YSFHYSSPVSSVIGAKIGNTLILMLSAMIVAYVIGILGGVLMAWYRGSKLETFGISAALIFRALPPFFVGMLFIMLFSFH
LGWLPHSGMRTAGYVAEGALDRFFNLDFLRHLILPMTVSALYFLAQPLLIMRNTMMEVMGEDFVEMADAKGLKKVRIMYV
HAARNALLPVVTQGALFLGMAVGGQVLIEYVFGWPGLGEEIVLAAQRHDYPVAQASFLMMASLVMLMNFLADLLYGYLDP
RVVYN
>Mature_324_residues
GTQQFILRRLIQMIISIFVVATVIFFMFRLLPGDPTTAMIEPGWSQEATQMLIERFGLDRPLHEQYFHYLVNLFQLDFGY
SFHYSSPVSSVIGAKIGNTLILMLSAMIVAYVIGILGGVLMAWYRGSKLETFGISAALIFRALPPFFVGMLFIMLFSFHL
GWLPHSGMRTAGYVAEGALDRFFNLDFLRHLILPMTVSALYFLAQPLLIMRNTMMEVMGEDFVEMADAKGLKKVRIMYVH
AARNALLPVVTQGALFLGMAVGGQVLIEYVFGWPGLGEEIVLAAQRHDYPVAQASFLMMASLVMLMNFLADLLYGYLDPR
VVYN

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=342, Percent_Identity=38.3040935672515, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787053, Length=321, Percent_Identity=31.7757009345794, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787497, Length=322, Percent_Identity=32.6086956521739, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789888, Length=322, Percent_Identity=31.6770186335404, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1788504, Length=358, Percent_Identity=25.9776536312849, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787761, Length=331, Percent_Identity=32.3262839879154, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787550, Length=272, Percent_Identity=25.7352941176471, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36590; Mature: 36459

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
7.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GTQQFILRRLIQMIISIFVVATVIFFMFRLLPGDPTTAMIEPGWSQEATQMLIERFGLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCC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HHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]