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The map label for this gene is gbsB [H]

Identifier: 188586664

GI number: 188586664

Start: 2171174

End: 2172349

Strand: Reverse

Name: gbsB [H]

Synonym: Nther_2054

Alternate gene names: 188586664

Gene position: 2172349-2171174 (Counterclockwise)

Preceding gene: 188586665

Following gene: 188586663

Centisome position: 68.62

GC content: 41.24

Gene sequence:

>1176_bases
ATGTTGAGATTTGAACAGATGCAGGAAAATGAGTTTGTATTGCCTACTAAAACTAAGCACGGATTAGGAATTATCAAGGA
AATTGCCAATGAGTTAAAGGAACTTAACGTAAGTAAGCCAATTATTGTAGCTGATAAGGGAGTTATTGATGCAGGACTTA
TCAAACCTATTGAAGAGTCATTAAATGAAGCAGGTATCCCCTATGCAGTTTATGATGGGGTAGAAGCAGATCCAGATTTG
GAAATAGTAGCCAATGGTACTAAAGCATATAAAAAAGAGAATTGCGATGGTATGATTGCTGTTGGTGGGGGAAGCTCCAT
GGATACTGCAAAGGCTATGGGACTAGAAATTTCTCATGATGGCCCTGTTGTAGAATATGAAGCCGCTGAGGGTAAGAAAC
CAATGACTAAAAGAATACCACCATTGGTGACTGTTCCTACGACTGCAGGAACCGGAAGTGAAGTTACCCTATGGGCTGTA
ATAAAAGACCCTGAGAGAGAATTTAAATTTAATACTGGTGGACCATTGATTACGGCTCATTTAGCATTGATTGATCCTGA
ATTACATGTTACAATGCCCCCATCTATTACAGCAGGAACAGGAATGGATGCACTTTGTCATGCTATAGAATGCTATACTT
GCCACTATTCTCAACCAACTACTGATGCAGCAGCACTTTTAGCCATTGAGTACGCAGGTAAATATCTCCGACGAGCAGTA
GGTAATGGTCAAGATATAGAAGCTCGTTATGGTATGGCTATGTCAGCTATGTTAGCAGGTATATCATACGGAGGCGATAG
TGCTGGAGCAGTTCATGCCATGACTCAAACACTAGGAGGAATTATCTCTGTACCTCATGGTCAGGCAGTAGCAGCTACCT
TAGCTCCTGCAATGGAGTATAACTGGATTGGAGCACCTCATAAGTTTGCGCGCATTGCCAATGCTCTAGGTGTAGATACT
CACGGTATGGACTTACATGAAGCTGCTAGGGCTTCTGTTGAGGCAGTGTATCAGCTATCTGAAGACATTGATGTGCCGAC
TTTAGGCGATTTAGGTGTATCAGAAGACATGATTCCTAGGCTTGCTAAAGAAGCTTATTATGATCCACAAACAGTTGGAA
ATCCAAGAGATATCGATGTAAAAGGATATGAAGAAATTTATCGTTCATGCTTCTAA

Upstream 100 bases:

>100_bases
AGAAGTTAGCCCAACTGGGCTTAAAAGATAAACTTTAAAATTAAACTTCAAAAACTAATTTGAAGTTTCTAAATTAAATT
TAATCAGGAGGGATTTTATT

Downstream 100 bases:

>100_bases
ATAAGGGATGATCAGTGATGAGTGACTTTAAAAATGGAAGCGCATTAAACCTAATTAAATAGTAATAACGTCAAATATGG
AGAAATTACCCCTGATTTAA

Product: iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MLRFEQMQENEFVLPTKTKHGLGIIKEIANELKELNVSKPIIVADKGVIDAGLIKPIEESLNEAGIPYAVYDGVEADPDL
EIVANGTKAYKKENCDGMIAVGGGSSMDTAKAMGLEISHDGPVVEYEAAEGKKPMTKRIPPLVTVPTTAGTGSEVTLWAV
IKDPEREFKFNTGGPLITAHLALIDPELHVTMPPSITAGTGMDALCHAIECYTCHYSQPTTDAAALLAIEYAGKYLRRAV
GNGQDIEARYGMAMSAMLAGISYGGDSAGAVHAMTQTLGGIISVPHGQAVAATLAPAMEYNWIGAPHKFARIANALGVDT
HGMDLHEAARASVEAVYQLSEDIDVPTLGDLGVSEDMIPRLAKEAYYDPQTVGNPRDIDVKGYEEIYRSCF

Sequences:

>Translated_391_residues
MLRFEQMQENEFVLPTKTKHGLGIIKEIANELKELNVSKPIIVADKGVIDAGLIKPIEESLNEAGIPYAVYDGVEADPDL
EIVANGTKAYKKENCDGMIAVGGGSSMDTAKAMGLEISHDGPVVEYEAAEGKKPMTKRIPPLVTVPTTAGTGSEVTLWAV
IKDPEREFKFNTGGPLITAHLALIDPELHVTMPPSITAGTGMDALCHAIECYTCHYSQPTTDAAALLAIEYAGKYLRRAV
GNGQDIEARYGMAMSAMLAGISYGGDSAGAVHAMTQTLGGIISVPHGQAVAATLAPAMEYNWIGAPHKFARIANALGVDT
HGMDLHEAARASVEAVYQLSEDIDVPTLGDLGVSEDMIPRLAKEAYYDPQTVGNPRDIDVKGYEEIYRSCF
>Mature_391_residues
MLRFEQMQENEFVLPTKTKHGLGIIKEIANELKELNVSKPIIVADKGVIDAGLIKPIEESLNEAGIPYAVYDGVEADPDL
EIVANGTKAYKKENCDGMIAVGGGSSMDTAKAMGLEISHDGPVVEYEAAEGKKPMTKRIPPLVTVPTTAGTGSEVTLWAV
IKDPEREFKFNTGGPLITAHLALIDPELHVTMPPSITAGTGMDALCHAIECYTCHYSQPTTDAAALLAIEYAGKYLRRAV
GNGQDIEARYGMAMSAMLAGISYGGDSAGAVHAMTQTLGGIISVPHGQAVAATLAPAMEYNWIGAPHKFARIANALGVDT
HGMDLHEAARASVEAVYQLSEDIDVPTLGDLGVSEDMIPRLAKEAYYDPQTVGNPRDIDVKGYEEIYRSCF

Specific function: Essential for the utilization of choline as a precursor [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=384, Percent_Identity=30.46875, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1789163, Length=386, Percent_Identity=36.2694300518135, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI48994951, Length=385, Percent_Identity=34.2857142857143, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87082107, Length=378, Percent_Identity=31.4814814814815, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1787493, Length=368, Percent_Identity=30.4347826086957, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789386, Length=391, Percent_Identity=24.2966751918159, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17537053, Length=407, Percent_Identity=27.5184275184275, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321181, Length=367, Percent_Identity=32.6975476839237, Blast_Score=189, Evalue=6e-49,
Organism=Drosophila melanogaster, GI24657991, Length=382, Percent_Identity=26.1780104712042, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.1 [H]

Molecular weight: Translated: 41749; Mature: 41749

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFEQMQENEFVLPTKTKHGLGIIKEIANELKELNVSKPIIVADKGVIDAGLIKPIEES
CCCCHHCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHH
LNEAGIPYAVYDGVEADPDLEIVANGTKAYKKENCDGMIAVGGGSSMDTAKAMGLEISHD
HHHCCCCEEEECCCCCCCCCEEEECCHHHHHHCCCCEEEEECCCCCCCHHHHHCEEECCC
GPVVEYEAAEGKKPMTKRIPPLVTVPTTAGTGSEVTLWAVIKDPEREFKFNTGGPLITAH
CCEEEEECCCCCCCHHHCCCCEEEECCCCCCCCCEEEEEEEECCCHHEEECCCCCEEEEE
LALIDPELHVTMPPSITAGTGMDALCHAIECYTCHYSQPTTDAAALLAIEYAGKYLRRAV
EEEECCCEEEECCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHH
GNGQDIEARYGMAMSAMLAGISYGGDSAGAVHAMTQTLGGIISVPHGQAVAATLAPAMEY
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEECCCCCHHHHHHHHHHCC
NWIGAPHKFARIANALGVDTHGMDLHEAARASVEAVYQLSEDIDVPTLGDLGVSEDMIPR
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LAKEAYYDPQTVGNPRDIDVKGYEEIYRSCF
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MLRFEQMQENEFVLPTKTKHGLGIIKEIANELKELNVSKPIIVADKGVIDAGLIKPIEES
CCCCHHCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHH
LNEAGIPYAVYDGVEADPDLEIVANGTKAYKKENCDGMIAVGGGSSMDTAKAMGLEISHD
HHHCCCCEEEECCCCCCCCCEEEECCHHHHHHCCCCEEEEECCCCCCCHHHHHCEEECCC
GPVVEYEAAEGKKPMTKRIPPLVTVPTTAGTGSEVTLWAVIKDPEREFKFNTGGPLITAH
CCEEEEECCCCCCCHHHCCCCEEEECCCCCCCCCEEEEEEEECCCHHEEECCCCCEEEEE
LALIDPELHVTMPPSITAGTGMDALCHAIECYTCHYSQPTTDAAALLAIEYAGKYLRRAV
EEEECCCEEEECCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHH
GNGQDIEARYGMAMSAMLAGISYGGDSAGAVHAMTQTLGGIISVPHGQAVAATLAPAMEY
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEECCCCCHHHHHHHHHHCC
NWIGAPHKFARIANALGVDTHGMDLHEAARASVEAVYQLSEDIDVPTLGDLGVSEDMIPR
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LAKEAYYDPQTVGNPRDIDVKGYEEIYRSCF
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8752328; 9384377 [H]