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The map label for this gene is opuAB [H]

Identifier: 188586657

GI number: 188586657

Start: 2162718

End: 2163566

Strand: Reverse

Name: opuAB [H]

Synonym: Nther_2047

Alternate gene names: 188586657

Gene position: 2163566-2162718 (Counterclockwise)

Preceding gene: 188586658

Following gene: 188586656

Centisome position: 68.35

GC content: 35.57

Gene sequence:

>849_bases
ATGAGAGAATTTCCTGATATCACCATAGAATTTGGGCAATATGTAGAGAGTTTTGTCAACTGGCTAGATACAAATTTAGA
GTTTTTATTTGATTTTATCTTTTATGTGATTCTCAATTTGATTTCTAATATTGAAGGTTTTTTGATTTGGCTACCTTGGT
GGTTATTTGTATTATTTATATTTTTACTTGGTTGGAAATTAAGAACAATTTCTTCAGGAGTATTATTTTCCGTGTTTTTA
TTTGTGATCGGGACTTTTGGTCTTTGGGTACCTATGATGGAAACACTGGCTATTATAGTAACATCAGTTGTTATATCCAT
AGTTTTAGGTATACCCATTGGGATTTTAATGGCTTATAACCGTATTATGTCAGTTGTTATCACACCGATTTTAGATGGTA
TGCAGACTATGCCTAGCTTTGTTTACTTAATACCGGCTATAATTCTGTTTCATATGGGGACAGTTCCAGCAGTATTTGCC
ACTATAATATATGCTATTCCACCAGTAATCAGATTGACTGATTTAGGTATAAGGAAAGTATCAAAGGAAATGGTAGAAGC
GGGTAAATCCTTTGGAGCTTCTCCGGTACAATTATTGGCCAAGGTACAAATACCCCAGGCCCTACCAACCATTTTAGCAG
GTACTAATCAAACTACTATGATGGCACTAGCTATGGTAGTAATTGCAGCTATGATAGGGGCTGATGGACTAGGAATGGAA
GTTTATATAGCAATCAACAGAATAGATGTAGCTCGGGGAGTAGAGGCTGGCCTTAGTATAGTATTTTTGGCCATTATAAT
AGACAGGCTCACCCAATCAATTGGGGATAAATTCAAATACACAGAGTAA

Upstream 100 bases:

>100_bases
ATATTTACAGCAATAATTGTATTTTAATAATAAGCACATAGTGAGGTGAGATAAACACTGCTCCCGTTGTAATCACTTCT
ACGGGAGCAGTCACAATCTT

Downstream 100 bases:

>100_bases
AGTTGACTGTGTAATACGAATCAAAATAAAAATAACTGGGAGTTTTGATAATGACCACTAATATGAGTGAAAAAATTAAA
GTTAAAAATTTGTCAAAGAT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MREFPDITIEFGQYVESFVNWLDTNLEFLFDFIFYVILNLISNIEGFLIWLPWWLFVLFIFLLGWKLRTISSGVLFSVFL
FVIGTFGLWVPMMETLAIIVTSVVISIVLGIPIGILMAYNRIMSVVITPILDGMQTMPSFVYLIPAIILFHMGTVPAVFA
TIIYAIPPVIRLTDLGIRKVSKEMVEAGKSFGASPVQLLAKVQIPQALPTILAGTNQTTMMALAMVVIAAMIGADGLGME
VYIAINRIDVARGVEAGLSIVFLAIIIDRLTQSIGDKFKYTE

Sequences:

>Translated_282_residues
MREFPDITIEFGQYVESFVNWLDTNLEFLFDFIFYVILNLISNIEGFLIWLPWWLFVLFIFLLGWKLRTISSGVLFSVFL
FVIGTFGLWVPMMETLAIIVTSVVISIVLGIPIGILMAYNRIMSVVITPILDGMQTMPSFVYLIPAIILFHMGTVPAVFA
TIIYAIPPVIRLTDLGIRKVSKEMVEAGKSFGASPVQLLAKVQIPQALPTILAGTNQTTMMALAMVVIAAMIGADGLGME
VYIAINRIDVARGVEAGLSIVFLAIIIDRLTQSIGDKFKYTE
>Mature_282_residues
MREFPDITIEFGQYVESFVNWLDTNLEFLFDFIFYVILNLISNIEGFLIWLPWWLFVLFIFLLGWKLRTISSGVLFSVFL
FVIGTFGLWVPMMETLAIIVTSVVISIVLGIPIGILMAYNRIMSVVITPILDGMQTMPSFVYLIPAIILFHMGTVPAVFA
TIIYAIPPVIRLTDLGIRKVSKEMVEAGKSFGASPVQLLAKVQIPQALPTILAGTNQTTMMALAMVVIAAMIGADGLGME
VYIAINRIDVARGVEAGLSIVFLAIIIDRLTQSIGDKFKYTE

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=279, Percent_Identity=41.5770609318996, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1788451, Length=167, Percent_Identity=28.1437125748503, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788449, Length=232, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31327; Mature: 31327

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]