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The map label for this gene is gshAB [H]

Identifier: 188586588

GI number: 188586588

Start: 2081867

End: 2083255

Strand: Direct

Name: gshAB [H]

Synonym: Nther_1975

Alternate gene names: 188586588

Gene position: 2081867-2083255 (Clockwise)

Preceding gene: 188586585

Following gene: 188586589

Centisome position: 65.77

GC content: 33.62

Gene sequence:

>1389_bases
ATGCTAAGAATAGGGAATAACCAAACTTCAAAAATTAAAAAACAAGAAAACAAATATACTAAAGCTAGGATGTTTTATAT
GAACATAAAAAACAATGAAGTATTAACATTGATCCGGAAGTCAGATATAAACCCTCTGAGTTTTGAAGGAAATTTCGGAT
TAGAAAAGGAGAATTTAAGAGTTGATGAACAAGGCAACCTGGCATTATCTCCTCATCCCAAGGAATTTGGTGATAAATTA
CGCAATCCTTATATAAAAACCGATTTTTCAGAAAGCCAGATAGAGCTTATCACTCCAGTTTGTAAAAGTATAGAAGAAGC
ATACGAGTTTCTAGGTGAACTTCAGGACTTTGTTTCGGTGAGATTAACCGGAGAATATTTATGGCCTCAAAGCAATCTGC
CTAAATTGCCAGAAGAAGAACAAATACCTATCGCAAGATATGGCAAGGAAGGTTATAAAGAAAGAAAATATAGAGAAAGA
TTAGCCAAAAAATACGGTAAAAAACTGCAATTACTATCCGGAGTTCACTTTAATTTCTCCCTTAAAGAAGAATTTTTAAA
TAAGTTGTATGCTAGGTTAGAACACAATAAATCTTATCAGGAATTCAAAAATGATATTTATCTAAAAATCACAAAGTATT
TATTGAAATACAGATGGCTGTTGATCTATCTAACAGGAGCCAGTCCAACTCTTAAAACTGCTGATAATTTAGGTTGCTAT
TGCAAAGATTTTCGTTCTCTGAGAAATAGTAGCTGCGGTTATAGTAATAATGAGGAACTTTATATCAGTTATGAAACGAT
CGAAGATTACGTTGCTAGCCTGCAGGCTTCCATTGATCAAGGTAAAATTCAAAACATTAATGAATTCTATTCACCAGTCA
GGATTAAAGGAAGAGGGAAAGGAACATTAGAAGAGCTGTTGAAGCAAGGAATTCAATATCTGGAATTGAGGCTCTTTGAT
TTGAATCCTTTCGACAAAAACGGAATTTCAAAAGAAGACCTTTATTTTACAAGACTATTGATTACCTATCTGCTTGTAAA
AGCAGATTTCACCTTTGGAACCATGGAACAGAAAATGGCTAATACAAATCATGAACAAGCAACTTTAGCTGAAAGACCTG
AAAATATTGGCGTTTTTGATGAGTTAGGTCAGTGGGAAAATTTGCAGGAAGAAGCCACAAAAATTATAAATGAACTTGAA
GAATTAATTGAAGTGCTTGGGGGTGAAACTGAGTATTTTTCTTATGTAATCAATACGGCTAGAGAAAAAGTGCTAAAACC
AGAAAAAACCTATGCCCTTAGGATTATTGAAGGAGTTAAACAAAGCTCCTATGTGCAGTTTCATATTGACCTGGCAAAAA
AGCATCTTGAAGAGAGCAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
TTACATTTCTATTTTGGGAATTAAAATCCTTTATTAATTATAAATATCATTCTAAAAAATGGAGGTTAGCATTTAATAGA
ATTTTGATTTAATATCGTAA

Downstream 100 bases:

>100_bases
ATAAGATGGGATAATCTCGTACTAATAAAAAAGTTTGCCGTTGTAGCTGTGACTTGAACCGTATCTTTGCCATTTTTTGA
ATCATAAGTTTGCCAAAAAA

Product: Glutathione synthase

Products: NA

Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MLRIGNNQTSKIKKQENKYTKARMFYMNIKNNEVLTLIRKSDINPLSFEGNFGLEKENLRVDEQGNLALSPHPKEFGDKL
RNPYIKTDFSESQIELITPVCKSIEEAYEFLGELQDFVSVRLTGEYLWPQSNLPKLPEEEQIPIARYGKEGYKERKYRER
LAKKYGKKLQLLSGVHFNFSLKEEFLNKLYARLEHNKSYQEFKNDIYLKITKYLLKYRWLLIYLTGASPTLKTADNLGCY
CKDFRSLRNSSCGYSNNEELYISYETIEDYVASLQASIDQGKIQNINEFYSPVRIKGRGKGTLEELLKQGIQYLELRLFD
LNPFDKNGISKEDLYFTRLLITYLLVKADFTFGTMEQKMANTNHEQATLAERPENIGVFDELGQWENLQEEATKIINELE
ELIEVLGGETEYFSYVINTAREKVLKPEKTYALRIIEGVKQSSYVQFHIDLAKKHLEESKKK

Sequences:

>Translated_462_residues
MLRIGNNQTSKIKKQENKYTKARMFYMNIKNNEVLTLIRKSDINPLSFEGNFGLEKENLRVDEQGNLALSPHPKEFGDKL
RNPYIKTDFSESQIELITPVCKSIEEAYEFLGELQDFVSVRLTGEYLWPQSNLPKLPEEEQIPIARYGKEGYKERKYRER
LAKKYGKKLQLLSGVHFNFSLKEEFLNKLYARLEHNKSYQEFKNDIYLKITKYLLKYRWLLIYLTGASPTLKTADNLGCY
CKDFRSLRNSSCGYSNNEELYISYETIEDYVASLQASIDQGKIQNINEFYSPVRIKGRGKGTLEELLKQGIQYLELRLFD
LNPFDKNGISKEDLYFTRLLITYLLVKADFTFGTMEQKMANTNHEQATLAERPENIGVFDELGQWENLQEEATKIINELE
ELIEVLGGETEYFSYVINTAREKVLKPEKTYALRIIEGVKQSSYVQFHIDLAKKHLEESKKK
>Mature_462_residues
MLRIGNNQTSKIKKQENKYTKARMFYMNIKNNEVLTLIRKSDINPLSFEGNFGLEKENLRVDEQGNLALSPHPKEFGDKL
RNPYIKTDFSESQIELITPVCKSIEEAYEFLGELQDFVSVRLTGEYLWPQSNLPKLPEEEQIPIARYGKEGYKERKYRER
LAKKYGKKLQLLSGVHFNFSLKEEFLNKLYARLEHNKSYQEFKNDIYLKITKYLLKYRWLLIYLTGASPTLKTADNLGCY
CKDFRSLRNSSCGYSNNEELYISYETIEDYVASLQASIDQGKIQNINEFYSPVRIKGRGKGTLEELLKQGIQYLELRLFD
LNPFDKNGISKEDLYFTRLLITYLLVKADFTFGTMEQKMANTNHEQATLAERPENIGVFDELGQWENLQEEATKIINELE
ELIEVLGGETEYFSYVINTAREKVLKPEKTYALRIIEGVKQSSYVQFHIDLAKKHLEESKKK

Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine [H]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=435, Percent_Identity=27.5862068965517, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR007370
- InterPro:   IPR006335
- InterPro:   IPR020561 [H]

Pfam domain/function: PF01071 GARS_A; PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2; =6.3.2.3 [H]

Molecular weight: Translated: 54029; Mature: 54029

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIGNNQTSKIKKQENKYTKARMFYMNIKNNEVLTLIRKSDINPLSFEGNFGLEKENLR
CEECCCCCHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCCEEECCCCCCCCCCCE
VDEQGNLALSPHPKEFGDKLRNPYIKTDFSESQIELITPVCKSIEEAYEFLGELQDFVSV
ECCCCCEEECCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEE
RLTGEYLWPQSNLPKLPEEEQIPIARYGKEGYKERKYRERLAKKYGKKLQLLSGVHFNFS
EEECEEECCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEE
LKEEFLNKLYARLEHNKSYQEFKNDIYLKITKYLLKYRWLLIYLTGASPTLKTADNLGCY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHH
CKDFRSLRNSSCGYSNNEELYISYETIEDYVASLQASIDQGKIQNINEFYSPVRIKGRGK
HHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEECCCC
GTLEELLKQGIQYLELRLFDLNPFDKNGISKEDLYFTRLLITYLLVKADFTFGTMEQKMA
CHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NTNHEQATLAERPENIGVFDELGQWENLQEEATKIINELEELIEVLGGETEYFSYVINTA
CCCCHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
REKVLKPEKTYALRIIEGVKQSSYVQFHIDLAKKHLEESKKK
HHHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRIGNNQTSKIKKQENKYTKARMFYMNIKNNEVLTLIRKSDINPLSFEGNFGLEKENLR
CEECCCCCHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCCEEECCCCCCCCCCCE
VDEQGNLALSPHPKEFGDKLRNPYIKTDFSESQIELITPVCKSIEEAYEFLGELQDFVSV
ECCCCCEEECCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEE
RLTGEYLWPQSNLPKLPEEEQIPIARYGKEGYKERKYRERLAKKYGKKLQLLSGVHFNFS
EEECEEECCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEE
LKEEFLNKLYARLEHNKSYQEFKNDIYLKITKYLLKYRWLLIYLTGASPTLKTADNLGCY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHH
CKDFRSLRNSSCGYSNNEELYISYETIEDYVASLQASIDQGKIQNINEFYSPVRIKGRGK
HHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEECCCC
GTLEELLKQGIQYLELRLFDLNPFDKNGISKEDLYFTRLLITYLLVKADFTFGTMEQKMA
CHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NTNHEQATLAERPENIGVFDELGQWENLQEEATKIINELEELIEVLGGETEYFSYVINTA
CCCCHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
REKVLKPEKTYALRIIEGVKQSSYVQFHIDLAKKHLEESKKK
HHHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]