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The map label for this gene is nfdA [H]

Identifier: 188586548

GI number: 188586548

Start: 2035139

End: 2036812

Strand: Direct

Name: nfdA [H]

Synonym: Nther_1934

Alternate gene names: 188586548

Gene position: 2035139-2036812 (Clockwise)

Preceding gene: 188586544

Following gene: 188586558

Centisome position: 64.29

GC content: 38.77

Gene sequence:

>1674_bases
TTGCACAATCATGATTTTACGGGTACATCCCCACATCTAATTCTGATTAATGGCAAGATAGCTACTGTTGACTCAAACAA
CAGTATTGCTGAAGCTATCGGTATTTTTGGTAATTTAATTTCTCATGTGGGAACAACACAAGAATTATTGAAAACTACGG
GACCTAACACTAAAGTGATCGATTTACAAGGTAAAACTGTAATTCCTGGTATTGTAGATTCTCATAATCATGTATATCAA
GCAGGGATTTTAATGGAAGGAGTAATGGCTTTCGGACTGGAAAGCATAAAAGAGCTACAAGAAGCTGTCAAAGCAAAGGT
TTCAGAAACCCCGAAAGGAAAGTGGATTCGGGGTGGTGGCTGGATTGAAAGTCAGTTCGCGGAAAACCGCATGCCAAACC
GCTGGGATTTAGATGAAGTGGCTCCCGATCATCCCGTGGTTCTATCAAGATTATTTGCCATGGATCTTTGTAACAGTAAA
GCTCTCGAATTAGCTGGAATAAATAAAAACACTCCTCAACCCCAGCGCGGAACAATTGATAAGGACCCGAAAACCGGGGA
ACCAACTGGTATTTTGCGAAATGGCGCTCAAGCTTTAGTAAGCCGACTTATTAAAGACGATTATAATTCCAATTATAATT
TTCAAGAATTAGTTGAAAGACGTGTTAAAACAGCCATGAATGAGTATCTACAGTATGGCATAACTACAGTTCTAGACCCT
GGTGTCAGCGTTCCAGTCATGAGAGGATACCAAAATCTATACAAAAAAGGTGAACTTCCAATCAGATTGCAAATGATGCC
GGAAGCTTACGGGCTAGCGGCTATTTCATCTAATAAAAGCGATCCCAATGAAACAGAGAAATTTATGGACTATTTGGGTA
TAAATGATCCTTTCGGCAATGAATGGTTCAGTATCGGGCCGTTAAAATTCGCTGTAGATGGTGGTGTAGGGTCAAAGACA
GCTTTGATGTACGAACCTTGGATAGATGGAACCAAGAGCAATATTCCCTTAAGACTTGATTTTGAAGAGATGGAAAAGTT
ATTTCATAAAGCTCAAGAATTAGGATGGTCCATTGGCATACACACTTGTGGAGATAAAGCTCAGGATATCGTGTTAGAAG
CTTTTAAAAAAGTCATCGACAGATACCCTAGAAATGATGTCCGACATAATATTATCCATGGATATTTTCCAACTGACCGT
GCTCTAGAAATAATGAAAGAATATAATATAGCCGGTTCCCTTCAACCCGGTTTCATGTACGTCGAAGGAGATCTGTACTG
GGATGTGTTAAACCAAGAGCAAATAGAGTATTTTACACCTATCAAAACTTACTTAAACAATAAAATTACTGTGGCATGTA
ATTCTGACATGATTTCAGCACATTACAACCCTTTTTATGGCATGTATTCCGCTGTAGCTCGCAAGACATCTCAAGGAAAA
AGCTTGGGTGCTCGAGAGAAAATTTCACGAGAAGAAATGCTTCGTCTATTTACAATTAACAGTGCCTATTTAACCTTTGA
AGAAAATACAAAAGGGTCTATAGAGGCCGGCAAACTAGCAGATATTGCGGTACTCTCCCATGATCTTCTAACATGTCCTG
AATCAGATATATTGAAGACCGATGTAAATATGACGATCATTGACGGAAAAATAGTTCATTCAAATATCAATTAA

Upstream 100 bases:

>100_bases
ATACAGGGTTTTTCTAAGTACTAAAGGATTTTAAAATTAATTACAGAATTTTTAATATAATATAAAAATGTGTTTATTTC
TCAATTTGGAGGTGCTTTTT

Downstream 100 bases:

>100_bases
TGAACCGACCGACTTTCACTTCGTATAATTTTATTTATTATTGGGCTAATATTTATATCGAAGGTTCCTTTACTAACCCA
TTTAACCGTAAAATACGGAT

Product: Amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MHNHDFTGTSPHLILINGKIATVDSNNSIAEAIGIFGNLISHVGTTQELLKTTGPNTKVIDLQGKTVIPGIVDSHNHVYQ
AGILMEGVMAFGLESIKELQEAVKAKVSETPKGKWIRGGGWIESQFAENRMPNRWDLDEVAPDHPVVLSRLFAMDLCNSK
ALELAGINKNTPQPQRGTIDKDPKTGEPTGILRNGAQALVSRLIKDDYNSNYNFQELVERRVKTAMNEYLQYGITTVLDP
GVSVPVMRGYQNLYKKGELPIRLQMMPEAYGLAAISSNKSDPNETEKFMDYLGINDPFGNEWFSIGPLKFAVDGGVGSKT
ALMYEPWIDGTKSNIPLRLDFEEMEKLFHKAQELGWSIGIHTCGDKAQDIVLEAFKKVIDRYPRNDVRHNIIHGYFPTDR
ALEIMKEYNIAGSLQPGFMYVEGDLYWDVLNQEQIEYFTPIKTYLNNKITVACNSDMISAHYNPFYGMYSAVARKTSQGK
SLGAREKISREEMLRLFTINSAYLTFEENTKGSIEAGKLADIAVLSHDLLTCPESDILKTDVNMTIIDGKIVHSNIN

Sequences:

>Translated_557_residues
MHNHDFTGTSPHLILINGKIATVDSNNSIAEAIGIFGNLISHVGTTQELLKTTGPNTKVIDLQGKTVIPGIVDSHNHVYQ
AGILMEGVMAFGLESIKELQEAVKAKVSETPKGKWIRGGGWIESQFAENRMPNRWDLDEVAPDHPVVLSRLFAMDLCNSK
ALELAGINKNTPQPQRGTIDKDPKTGEPTGILRNGAQALVSRLIKDDYNSNYNFQELVERRVKTAMNEYLQYGITTVLDP
GVSVPVMRGYQNLYKKGELPIRLQMMPEAYGLAAISSNKSDPNETEKFMDYLGINDPFGNEWFSIGPLKFAVDGGVGSKT
ALMYEPWIDGTKSNIPLRLDFEEMEKLFHKAQELGWSIGIHTCGDKAQDIVLEAFKKVIDRYPRNDVRHNIIHGYFPTDR
ALEIMKEYNIAGSLQPGFMYVEGDLYWDVLNQEQIEYFTPIKTYLNNKITVACNSDMISAHYNPFYGMYSAVARKTSQGK
SLGAREKISREEMLRLFTINSAYLTFEENTKGSIEAGKLADIAVLSHDLLTCPESDILKTDVNMTIIDGKIVHSNIN
>Mature_557_residues
MHNHDFTGTSPHLILINGKIATVDSNNSIAEAIGIFGNLISHVGTTQELLKTTGPNTKVIDLQGKTVIPGIVDSHNHVYQ
AGILMEGVMAFGLESIKELQEAVKAKVSETPKGKWIRGGGWIESQFAENRMPNRWDLDEVAPDHPVVLSRLFAMDLCNSK
ALELAGINKNTPQPQRGTIDKDPKTGEPTGILRNGAQALVSRLIKDDYNSNYNFQELVERRVKTAMNEYLQYGITTVLDP
GVSVPVMRGYQNLYKKGELPIRLQMMPEAYGLAAISSNKSDPNETEKFMDYLGINDPFGNEWFSIGPLKFAVDGGVGSKT
ALMYEPWIDGTKSNIPLRLDFEEMEKLFHKAQELGWSIGIHTCGDKAQDIVLEAFKKVIDRYPRNDVRHNIIHGYFPTDR
ALEIMKEYNIAGSLQPGFMYVEGDLYWDVLNQEQIEYFTPIKTYLNNKITVACNSDMISAHYNPFYGMYSAVARKTSQGK
SLGAREKISREEMLRLFTINSAYLTFEENTKGSIEAGKLADIAVLSHDLLTCPESDILKTDVNMTIIDGKIVHSNIN

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 62237; Mature: 62237

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNHDFTGTSPHLILINGKIATVDSNNSIAEAIGIFGNLISHVGTTQELLKTTGPNTKVI
CCCCCCCCCCCEEEEEECEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEE
DLQGKTVIPGIVDSHNHVYQAGILMEGVMAFGLESIKELQEAVKAKVSETPKGKWIRGGG
EECCCEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
WIESQFAENRMPNRWDLDEVAPDHPVVLSRLFAMDLCNSKALELAGINKNTPQPQRGTID
CCHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCC
KDPKTGEPTGILRNGAQALVSRLIKDDYNSNYNFQELVERRVKTAMNEYLQYGITTVLDP
CCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
GVSVPVMRGYQNLYKKGELPIRLQMMPEAYGLAAISSNKSDPNETEKFMDYLGINDPFGN
CCCCHHHHHHHHHHHCCCCCEEEEECCCHHCEEEECCCCCCCHHHHHHHHHHCCCCCCCC
EWFSIGPLKFAVDGGVGSKTALMYEPWIDGTKSNIPLRLDFEEMEKLFHKAQELGWSIGI
CCEEECCEEEEEECCCCCCEEEEEECCCCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEE
HTCGDKAQDIVLEAFKKVIDRYPRNDVRHNIIHGYFPTDRALEIMKEYNIAGSLQPGFMY
ECCCCHHHHHHHHHHHHHHHHCCCCCHHHCCEECCCCCHHHHHHHHHCCCCCCCCCCEEE
VEGDLYWDVLNQEQIEYFTPIKTYLNNKITVACNSDMISAHYNPFYGMYSAVARKTSQGK
EECCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHCCC
SLGAREKISREEMLRLFTINSAYLTFEENTKGSIEAGKLADIAVLSHDLLTCPESDILKT
CCCHHHHHCHHHHHEEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
DVNMTIIDGKIVHSNIN
CCCEEEEECEEEECCCC
>Mature Secondary Structure
MHNHDFTGTSPHLILINGKIATVDSNNSIAEAIGIFGNLISHVGTTQELLKTTGPNTKVI
CCCCCCCCCCCEEEEEECEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEE
DLQGKTVIPGIVDSHNHVYQAGILMEGVMAFGLESIKELQEAVKAKVSETPKGKWIRGGG
EECCCEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
WIESQFAENRMPNRWDLDEVAPDHPVVLSRLFAMDLCNSKALELAGINKNTPQPQRGTID
CCHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCC
KDPKTGEPTGILRNGAQALVSRLIKDDYNSNYNFQELVERRVKTAMNEYLQYGITTVLDP
CCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
GVSVPVMRGYQNLYKKGELPIRLQMMPEAYGLAAISSNKSDPNETEKFMDYLGINDPFGN
CCCCHHHHHHHHHHHCCCCCEEEEECCCHHCEEEECCCCCCCHHHHHHHHHHCCCCCCCC
EWFSIGPLKFAVDGGVGSKTALMYEPWIDGTKSNIPLRLDFEEMEKLFHKAQELGWSIGI
CCEEECCEEEEEECCCCCCEEEEEECCCCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEE
HTCGDKAQDIVLEAFKKVIDRYPRNDVRHNIIHGYFPTDRALEIMKEYNIAGSLQPGFMY
ECCCCHHHHHHHHHHHHHHHHCCCCCHHHCCEECCCCCHHHHHHHHHCCCCCCCCCCEEE
VEGDLYWDVLNQEQIEYFTPIKTYLNNKITVACNSDMISAHYNPFYGMYSAVARKTSQGK
EECCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHCCC
SLGAREKISREEMLRLFTINSAYLTFEENTKGSIEAGKLADIAVLSHDLLTCPESDILKT
CCCHHHHHCHHHHHEEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
DVNMTIIDGKIVHSNIN
CCCEEEEECEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA