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The map label for this gene is tpl [H]

Identifier: 188586417

GI number: 188586417

Start: 1865992

End: 1867368

Strand: Reverse

Name: tpl [H]

Synonym: Nther_1802

Alternate gene names: 188586417

Gene position: 1867368-1865992 (Counterclockwise)

Preceding gene: 188586418

Following gene: 188586416

Centisome position: 58.99

GC content: 37.4

Gene sequence:

>1377_bases
ATGTCAAGAGAGTTTCAAGCAGAACCTTTCAGAATTAAAACAGTAGAGCCAATCAAAATGACTACAAAAGAAGAGCGAGA
AGAGGCTATACAAAAAGCAGGGTTCAACACTTTCCTATTAGATTCTAAAGATGTTTACATCGATTTATTAACTGACAGCG
GAACTAATGCCATGAGTGATTATCAATGGGCAGGTCTTATGATGGGTGATGAGGCTTATGCCGGAAGTAAAAACTGGTAT
AACTTAGAAAAAGCAGTACAAGAATGTTTTGGTTTCGAATATGTAGTTCCAACTCATCAAGGTAGGGGCGCAGAAAACAT
TTTATCTCAGATCATGATTAAAGAGGGAGATTACATCCCAGGAAATATGTATTTTACAACTACTAAGGCCCATCAAGAAT
TTGCCGGAGGTACTTTTAGAGATGTGATAATTGACAAGGCACATGATTCACAAGCTGAGCATCCTTTTAAGGGAAATGTA
GACCTAGAAAAATATCAAGCTTTGATTGATGAAGTAGGTCCGGAACGAATTCCATATATCTGTGTTGCAGTTACTGTAAA
TATGGCGGGTGGACAGCCTGTTAGCATGGAAAATTTACGCAAGGTTAAAGAAATATCTGATAAATATGGTATAAAAGTAA
TGTTTGATGCTACTCGTTGTGTGGAAAATGCTTACTTTATCAAAGAAAGAGAAGAAGGGTATCAGGATAAATCCATTGCT
GAAATTTTAAAAGAAATGATGAGTTATGGTGACGGAGCTACTATGAGTGGTAAAAAAGATCCCTTAGTAAATATTGGTGG
TTTCTTGGCTATGAATGACGAAGAGCTATATCAAAGAGCTACTCAACTAGTTGTTGTGTACGAGGGAATGCCCACTTATG
GTGGAATGGCAGGAAGAGATATGGAAGCCATGGCGAGAGGAATATATGAATCACTAGATTATCACTACATTAGTCACAGA
GTCAATCAGGTAAGGTATTTAGGAGAGAAGCTAGAGGCTGGCGGAGTTCCAATTGTCAAACCTATCGGTGGTCATGCAAT
ATTTGTAGATGCCGAGAGATTCTTGTCCCATTTACCTAGAGAAGAATTTCCAGCCCAGGCCTTGGCTGCTAATATTTATA
GAGATTCTGGAGTAAGGACTATGGAAAGAGGTACTGTTTCTGCTGGAAGAGATAAAGATGGCAACAATATTTTCCCCAAA
TTAGAAACAGTTAGATTAACTATTCCTAGAAGAGTTTACACTTATGCGCATATGGACGTTGTTGCGGACTCGATTATCAA
TCTTTATGAAAATAGAGAAAAAATCAAAGGTTTACGATTTGCTTATGAACCACCAGTATTAAGATTCTTTACCGCAAAAT
TTGAAGAAATAAAATAA

Upstream 100 bases:

>100_bases
AGATGAAGTGGCTGCCGAAAAAGAATTATGATAGTTGTTTGCTTAGCTAAGCCCTTGATAATAGGGTTTATATAATTAAT
ACACTAAGGAGGTTATTTAC

Downstream 100 bases:

>100_bases
CGTTTGAACAAGAACTAAGAGCCCTCCAATGAGGGCTCTTTTGTTTTCGTTGCATACATGTAGAATATGATGTAAAATGG
AACATGACTTACTGGAAAAA

Product: tyrosine phenol-lyase

Products: NA

Alternate protein names: Beta-tyrosinase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSREFQAEPFRIKTVEPIKMTTKEEREEAIQKAGFNTFLLDSKDVYIDLLTDSGTNAMSDYQWAGLMMGDEAYAGSKNWY
NLEKAVQECFGFEYVVPTHQGRGAENILSQIMIKEGDYIPGNMYFTTTKAHQEFAGGTFRDVIIDKAHDSQAEHPFKGNV
DLEKYQALIDEVGPERIPYICVAVTVNMAGGQPVSMENLRKVKEISDKYGIKVMFDATRCVENAYFIKEREEGYQDKSIA
EILKEMMSYGDGATMSGKKDPLVNIGGFLAMNDEELYQRATQLVVVYEGMPTYGGMAGRDMEAMARGIYESLDYHYISHR
VNQVRYLGEKLEAGGVPIVKPIGGHAIFVDAERFLSHLPREEFPAQALAANIYRDSGVRTMERGTVSAGRDKDGNNIFPK
LETVRLTIPRRVYTYAHMDVVADSIINLYENREKIKGLRFAYEPPVLRFFTAKFEEIK

Sequences:

>Translated_458_residues
MSREFQAEPFRIKTVEPIKMTTKEEREEAIQKAGFNTFLLDSKDVYIDLLTDSGTNAMSDYQWAGLMMGDEAYAGSKNWY
NLEKAVQECFGFEYVVPTHQGRGAENILSQIMIKEGDYIPGNMYFTTTKAHQEFAGGTFRDVIIDKAHDSQAEHPFKGNV
DLEKYQALIDEVGPERIPYICVAVTVNMAGGQPVSMENLRKVKEISDKYGIKVMFDATRCVENAYFIKEREEGYQDKSIA
EILKEMMSYGDGATMSGKKDPLVNIGGFLAMNDEELYQRATQLVVVYEGMPTYGGMAGRDMEAMARGIYESLDYHYISHR
VNQVRYLGEKLEAGGVPIVKPIGGHAIFVDAERFLSHLPREEFPAQALAANIYRDSGVRTMERGTVSAGRDKDGNNIFPK
LETVRLTIPRRVYTYAHMDVVADSIINLYENREKIKGLRFAYEPPVLRFFTAKFEEIK
>Mature_457_residues
SREFQAEPFRIKTVEPIKMTTKEEREEAIQKAGFNTFLLDSKDVYIDLLTDSGTNAMSDYQWAGLMMGDEAYAGSKNWYN
LEKAVQECFGFEYVVPTHQGRGAENILSQIMIKEGDYIPGNMYFTTTKAHQEFAGGTFRDVIIDKAHDSQAEHPFKGNVD
LEKYQALIDEVGPERIPYICVAVTVNMAGGQPVSMENLRKVKEISDKYGIKVMFDATRCVENAYFIKEREEGYQDKSIAE
ILKEMMSYGDGATMSGKKDPLVNIGGFLAMNDEELYQRATQLVVVYEGMPTYGGMAGRDMEAMARGIYESLDYHYISHRV
NQVRYLGEKLEAGGVPIVKPIGGHAIFVDAERFLSHLPREEFPAQALAANIYRDSGVRTMERGTVSAGRDKDGNNIFPKL
ETVRLTIPRRVYTYAHMDVVADSIINLYENREKIKGLRFAYEPPVLRFFTAKFEEIK

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family [H]

Homologues:

Organism=Escherichia coli, GI87082323, Length=464, Percent_Identity=47.198275862069, Blast_Score=434, Evalue=1e-123,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR018176
- InterPro:   IPR013441 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: =4.1.99.2 [H]

Molecular weight: Translated: 51888; Mature: 51756

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREFQAEPFRIKTVEPIKMTTKEEREEAIQKAGFNTFLLDSKDVYIDLLTDSGTNAMSD
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCC
YQWAGLMMGDEAYAGSKNWYNLEKAVQECFGFEYVVPTHQGRGAENILSQIMIKEGDYIP
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCC
GNMYFTTTKAHQEFAGGTFRDVIIDKAHDSQAEHPFKGNVDLEKYQALIDEVGPERIPYI
CCEEEEEEHHHHHHCCCCCHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
CVAVTVNMAGGQPVSMENLRKVKEISDKYGIKVMFDATRCVENAYFIKEREEGYQDKSIA
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHEEHHHHCCCCCHHHH
EILKEMMSYGDGATMSGKKDPLVNIGGFLAMNDEELYQRATQLVVVYEGMPTYGGMAGRD
HHHHHHHHCCCCCCCCCCCCCCEECCCEEEECHHHHHHHHHEEEEEEECCCCCCCCCCCC
MEAMARGIYESLDYHYISHRVNQVRYLGEKLEAGGVPIVKPIGGHAIFVDAERFLSHLPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEHHHHHHHCCC
EEFPAQALAANIYRDSGVRTMERGTVSAGRDKDGNNIFPKLETVRLTIPRRVYTYAHMDV
CCCCHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHH
VADSIINLYENREKIKGLRFAYEPPVLRFFTAKFEEIK
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SREFQAEPFRIKTVEPIKMTTKEEREEAIQKAGFNTFLLDSKDVYIDLLTDSGTNAMSD
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCC
YQWAGLMMGDEAYAGSKNWYNLEKAVQECFGFEYVVPTHQGRGAENILSQIMIKEGDYIP
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCC
GNMYFTTTKAHQEFAGGTFRDVIIDKAHDSQAEHPFKGNVDLEKYQALIDEVGPERIPYI
CCEEEEEEHHHHHHCCCCCHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
CVAVTVNMAGGQPVSMENLRKVKEISDKYGIKVMFDATRCVENAYFIKEREEGYQDKSIA
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHEEHHHHCCCCCHHHH
EILKEMMSYGDGATMSGKKDPLVNIGGFLAMNDEELYQRATQLVVVYEGMPTYGGMAGRD
HHHHHHHHCCCCCCCCCCCCCCEECCCEEEECHHHHHHHHHEEEEEEECCCCCCCCCCCC
MEAMARGIYESLDYHYISHRVNQVRYLGEKLEAGGVPIVKPIGGHAIFVDAERFLSHLPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEHHHHHHHCCC
EEFPAQALAANIYRDSGVRTMERGTVSAGRDKDGNNIFPKLETVRLTIPRRVYTYAHMDV
CCCCHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHH
VADSIINLYENREKIKGLRFAYEPPVLRFFTAKFEEIK
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]