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The map label for this gene is ydeM [C]

Identifier: 188586401

GI number: 188586401

Start: 1852974

End: 1854443

Strand: Reverse

Name: ydeM [C]

Synonym: Nther_1786

Alternate gene names: 188586401

Gene position: 1854443-1852974 (Counterclockwise)

Preceding gene: 188586402

Following gene: 188586400

Centisome position: 58.58

GC content: 32.52

Gene sequence:

>1470_bases
ATGAGAAATTTTAAACCCTTAATTCACATATTCAATGTAGCTGATAGATACTTTTTATTTGACACAAATGCAGAGACTTT
TTTAGAGATCAGTAAAACTTGTTATCAGATAATTGAAGAAGCCCGACAAAATATAGGTGATAGGGGATTACTCACAAAAG
AAAGCTTTTCAGAGTTGATCAACTCTGAAATAGAGGACATAATTAGGCTTAGTAATCTTAATTATCAAGATGCTAATCAA
ATAGAAGAAACTATCACGGAATTGAAGATGCTTTATGAACAAGGTTTAATCTTTTCTGAACTACAATATCCTGTGGATGA
AAAAAATATAGGAAAGTCTGGTATCAAAGCTCTATGCGTACACCCTGCTCATGATTGTAATATGCGTTGTAGTTACTGTT
TTGCTGAAGGTGGATCTTATTCCCATGATATAAGCTATATGGATATCGATACTGCAAAACAAGCTGTTGAATTTTTAGTG
AATAATTCTGGAGACAGAAAAAACTTAGAGATAGATTTCTTTGGTGGAGAACCTTTATTAAATTTTCCGGTAATTGAAAA
ATGCGTTGCTCATGCAAAGGAGTTAGCTAACAAGCATGGGAAGCATTTTAAATTTACTTTAACTACCAATGGACTTGCAT
TAACTGATTCAATTATTGATTTTTTAGTTTCAAATCGTTTTGCCGTAGTTATGAGTTTAGATGGAAGAAAAGAAATCCAT
GATAATTATAGAACTGATCTGGCAGGTAATAGTACCTACGAAAAGATTTTACCTGCTTTTAAAAAATTATTAAATAAAAT
GCAGGCTCACGATTACTGTGAGTATTATATCAGGGGAACTTACACTCGCAAAAACTTAGATTTTTCCGAGGATGTGTTTC
ATATTGCTGATTTAGGTTTTAGCAGGATATCTTTAGAACCTGTTACTGGTGAAGAGAATTCAAGTTTTAGTTTAAATGAA
CATTATTTAAGTAAGTTGAAAAGTGAATACTTAAAGATAGTTGAAAACAGTTTAACAAGAAATAACTCTTCAGAGATTAA
CTTTTTTCACTTTAACCTAGATCTTAATAATGGACCATGTACTACCAAAAGAATTACAGGGTGTGGAGCAGGTTTTGATT
ATTTGGCTGTTGACCCTGGAGGAAATTTATATCCATGTCATCAATTTGTTGATATGGAAGAATATTCCATGGGTCATGTA
GCTGAGAATAGCTTAAACTCTGATATTTCTAAGAAATTCAAAACTAACAATTTGATAAATAAAAACGATTGCTATGATTG
CTGGGCTAAATTTTTATGTGGTGGAGGTTGTCATGCTAGTGCCCTAAAAGCTAATAACTCAATCGAAATGCCAAACCAGC
TCGATTGTCAGTTAAAGAAAATTCAGTTTGAATATGCTCTGTACAAAAAAGCGCGTGAACAGGAAGTAGATAAAGAAGGG
GTAGTTCATCATGGATATGGATCTGTTTAA

Upstream 100 bases:

>100_bases
CAAGACTTCATGTACTGTAGGAAATCAGGCATGCGAGCAAAAATAAATGATATAAGGTGATAATGAAAACGAATTGAAAA
TTGAAAGTGAGTTGAACAGC

Downstream 100 bases:

>100_bases
TTATAATTTAGAAGAAAAAAAGTTTAATGACACTCCGCTGGCGGACAGGATGCGGCCTCGTAGCCTTGACGACTTCGTGG
GACAGGAACAAATCTTAGGA

Product: Radical SAM domain protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MRNFKPLIHIFNVADRYFLFDTNAETFLEISKTCYQIIEEARQNIGDRGLLTKESFSELINSEIEDIIRLSNLNYQDANQ
IEETITELKMLYEQGLIFSELQYPVDEKNIGKSGIKALCVHPAHDCNMRCSYCFAEGGSYSHDISYMDIDTAKQAVEFLV
NNSGDRKNLEIDFFGGEPLLNFPVIEKCVAHAKELANKHGKHFKFTLTTNGLALTDSIIDFLVSNRFAVVMSLDGRKEIH
DNYRTDLAGNSTYEKILPAFKKLLNKMQAHDYCEYYIRGTYTRKNLDFSEDVFHIADLGFSRISLEPVTGEENSSFSLNE
HYLSKLKSEYLKIVENSLTRNNSSEINFFHFNLDLNNGPCTTKRITGCGAGFDYLAVDPGGNLYPCHQFVDMEEYSMGHV
AENSLNSDISKKFKTNNLINKNDCYDCWAKFLCGGGCHASALKANNSIEMPNQLDCQLKKIQFEYALYKKAREQEVDKEG
VVHHGYGSV

Sequences:

>Translated_489_residues
MRNFKPLIHIFNVADRYFLFDTNAETFLEISKTCYQIIEEARQNIGDRGLLTKESFSELINSEIEDIIRLSNLNYQDANQ
IEETITELKMLYEQGLIFSELQYPVDEKNIGKSGIKALCVHPAHDCNMRCSYCFAEGGSYSHDISYMDIDTAKQAVEFLV
NNSGDRKNLEIDFFGGEPLLNFPVIEKCVAHAKELANKHGKHFKFTLTTNGLALTDSIIDFLVSNRFAVVMSLDGRKEIH
DNYRTDLAGNSTYEKILPAFKKLLNKMQAHDYCEYYIRGTYTRKNLDFSEDVFHIADLGFSRISLEPVTGEENSSFSLNE
HYLSKLKSEYLKIVENSLTRNNSSEINFFHFNLDLNNGPCTTKRITGCGAGFDYLAVDPGGNLYPCHQFVDMEEYSMGHV
AENSLNSDISKKFKTNNLINKNDCYDCWAKFLCGGGCHASALKANNSIEMPNQLDCQLKKIQFEYALYKKAREQEVDKEG
VVHHGYGSV
>Mature_489_residues
MRNFKPLIHIFNVADRYFLFDTNAETFLEISKTCYQIIEEARQNIGDRGLLTKESFSELINSEIEDIIRLSNLNYQDANQ
IEETITELKMLYEQGLIFSELQYPVDEKNIGKSGIKALCVHPAHDCNMRCSYCFAEGGSYSHDISYMDIDTAKQAVEFLV
NNSGDRKNLEIDFFGGEPLLNFPVIEKCVAHAKELANKHGKHFKFTLTTNGLALTDSIIDFLVSNRFAVVMSLDGRKEIH
DNYRTDLAGNSTYEKILPAFKKLLNKMQAHDYCEYYIRGTYTRKNLDFSEDVFHIADLGFSRISLEPVTGEENSSFSLNE
HYLSKLKSEYLKIVENSLTRNNSSEINFFHFNLDLNNGPCTTKRITGCGAGFDYLAVDPGGNLYPCHQFVDMEEYSMGHV
AENSLNSDISKKFKTNNLINKNDCYDCWAKFLCGGGCHASALKANNSIEMPNQLDCQLKKIQFEYALYKKAREQEVDKEG
VVHHGYGSV

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemical mechanism initiated via the reduc

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=343, Percent_Identity=24.7813411078717, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994973, Length=347, Percent_Identity=23.6311239193084, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 55693; Mature: 55693

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNFKPLIHIFNVADRYFLFDTNAETFLEISKTCYQIIEEARQNIGDRGLLTKESFSELI
CCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
NSEIEDIIRLSNLNYQDANQIEETITELKMLYEQGLIFSELQYPVDEKNIGKSGIKALCV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCEEEEE
HPAHDCNMRCSYCFAEGGSYSHDISYMDIDTAKQAVEFLVNNSGDRKNLEIDFFGGEPLL
CCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
NFPVIEKCVAHAKELANKHGKHFKFTLTTNGLALTDSIIDFLVSNRFAVVMSLDGRKEIH
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEHHHHHHHHHHCCCEEEEEECCCCHHHH
DNYRTDLAGNSTYEKILPAFKKLLNKMQAHDYCEYYIRGTYTRKNLDFSEDVFHIADLGF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCC
SRISLEPVTGEENSSFSLNEHYLSKLKSEYLKIVENSLTRNNSSEINFFHFNLDLNNGPC
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCC
TTKRITGCGAGFDYLAVDPGGNLYPCHQFVDMEEYSMGHVAENSLNSDISKKFKTNNLIN
CCCEECCCCCCCCEEEECCCCCEECHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCC
KNDCYDCWAKFLCGGGCHASALKANNSIEMPNQLDCQLKKIQFEYALYKKAREQEVDKEG
CCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VVHHGYGSV
CEECCCCCC
>Mature Secondary Structure
MRNFKPLIHIFNVADRYFLFDTNAETFLEISKTCYQIIEEARQNIGDRGLLTKESFSELI
CCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
NSEIEDIIRLSNLNYQDANQIEETITELKMLYEQGLIFSELQYPVDEKNIGKSGIKALCV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCEEEEE
HPAHDCNMRCSYCFAEGGSYSHDISYMDIDTAKQAVEFLVNNSGDRKNLEIDFFGGEPLL
CCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
NFPVIEKCVAHAKELANKHGKHFKFTLTTNGLALTDSIIDFLVSNRFAVVMSLDGRKEIH
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEHHHHHHHHHHCCCEEEEEECCCCHHHH
DNYRTDLAGNSTYEKILPAFKKLLNKMQAHDYCEYYIRGTYTRKNLDFSEDVFHIADLGF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCC
SRISLEPVTGEENSSFSLNEHYLSKLKSEYLKIVENSLTRNNSSEINFFHFNLDLNNGPC
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCC
TTKRITGCGAGFDYLAVDPGGNLYPCHQFVDMEEYSMGHVAENSLNSDISKKFKTNNLIN
CCCEECCCCCCCCEEEECCCCCEECHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCC
KNDCYDCWAKFLCGGGCHASALKANNSIEMPNQLDCQLKKIQFEYALYKKAREQEVDKEG
CCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VVHHGYGSV
CEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA