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The map label for this gene is aprX [H]

Identifier: 188586243

GI number: 188586243

Start: 1706686

End: 1707984

Strand: Direct

Name: aprX [H]

Synonym: Nther_1626

Alternate gene names: 188586243

Gene position: 1706686-1707984 (Clockwise)

Preceding gene: 188586233

Following gene: 188586244

Centisome position: 53.91

GC content: 37.03

Gene sequence:

>1299_bases
ATGATAAGAGATAGTGCTTGGATACCAAATTACAATTATAAATTATGCCCCAAGTTAAAAAATGAGCTATACGAGAATTA
TAAGCCATCTAAGATGGTGCCTACTGTTTTTTACAATTCATATAAGCAGATAAAGAAAAAAATTCGTAAGTTTCCTGTGA
TCGTACAAGTTAAATTTACTAAGGATAATCTGACAATTATGGACCAAGTAGCAAGTCTGGCTCAATGTCGTATTAAGAAG
AAATATTATACTATTTTCTCTTTTTCTACCGACCTCACTGAAGTGCAATTAAAGAAATTGCTTGCAGATGACAAAGTTAT
TAAAGTTTGGTACGACGGCGAAGTCACTGGATTACTCGAAAAAGCTTCTAAAACAGTAAATGCCAATAAACTGTGGGATT
TAGAATTGACTGGAGAGAAAGTTACAATTGCTGTCTTGGATACTGGTGTTTATGAACATCCTTGTTTTGAAAATAGAATC
AAAAAGTTTCGGGATTTCGTAAATAATGGCAAGAAAACTTACGATGACAACGGGCACGGAACTCATGTAGCAGGTTGCAT
TGCTAGCAAACAAAATGAAGATTTTGGAGGGGTAGCCCCAAAGGCTGAAATTGTAGGAGTAAAAGTTTTAAACAAACTTG
GGTCTGGCAGACTTTCAAATGTTTTAGATGGAATCCAATGGTGTATTGAAAACAAAGAAAATCTAAACTTACAAATTATG
AATTTATCCCTTGGTTTTCCTACTCAAGAGTCTTACCGTGACGATCCTCTTTGCCAAATGGTTGAAAAGGCTTGGGAAGC
AGGTATTGTTGTCTGCGCTGCTGCCGGCAACAGCGGACCAGATAACGAAACTATAAATTCGCCAGGTATTCATCCTCAGA
TTATTACTGTTGGTGCTTCAAATGATAGGAATGCTGATTATCCCGAAGATAATATAATAGCTGATTTTTCAAGTCGTGGA
CCCACTAGAGATGGTCACAAAAAACCCGATGTAGTCGCCCCTGGAGTTGACATAGTTTCATTAAGATCTCCAAGTTCATT
CCTAGATAAAGTTGAACCAGGTTCTAGAATAGGTGAAGATCGTTTCATCTTCTCTGGAACTTCTATGGCTACTCCTATTT
GTGTGGGAGCTATAGCACAACTACTTCATTATAATAAAGACCTTGAGCCAGATGAGATAAAGTCATTGTTGATGGGTAAT
GCCAGGGAAATTCCCGGTGTGAATAGAGAAAGTCAAGGTACAGGTATCATAGATTCCAAAGAAATTCAAAGCAATATCAG
GGAATTTTCTGATAGTTGA

Upstream 100 bases:

>100_bases
AAAATTTATAAAATCTCATTTAATCTCGAAAAGTTAACAAATTTTTTCCCCTCTACATAAAGTGAATTATTACAATTTAT
ATGTATGGAGGGGGAACTAA

Downstream 100 bases:

>100_bases
GATAACATTAGTTTCCTTCATCAGAAAAAGTTCACTATTCAAAAGTGAATAGCATAGTTTGTAATAAAAATCAGGAGGTG
ATTTTTTGAGTAGACACTGG

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIRDSAWIPNYNYKLCPKLKNELYENYKPSKMVPTVFYNSYKQIKKKIRKFPVIVQVKFTKDNLTIMDQVASLAQCRIKK
KYYTIFSFSTDLTEVQLKKLLADDKVIKVWYDGEVTGLLEKASKTVNANKLWDLELTGEKVTIAVLDTGVYEHPCFENRI
KKFRDFVNNGKKTYDDNGHGTHVAGCIASKQNEDFGGVAPKAEIVGVKVLNKLGSGRLSNVLDGIQWCIENKENLNLQIM
NLSLGFPTQESYRDDPLCQMVEKAWEAGIVVCAAAGNSGPDNETINSPGIHPQIITVGASNDRNADYPEDNIIADFSSRG
PTRDGHKKPDVVAPGVDIVSLRSPSSFLDKVEPGSRIGEDRFIFSGTSMATPICVGAIAQLLHYNKDLEPDEIKSLLMGN
AREIPGVNRESQGTGIIDSKEIQSNIREFSDS

Sequences:

>Translated_432_residues
MIRDSAWIPNYNYKLCPKLKNELYENYKPSKMVPTVFYNSYKQIKKKIRKFPVIVQVKFTKDNLTIMDQVASLAQCRIKK
KYYTIFSFSTDLTEVQLKKLLADDKVIKVWYDGEVTGLLEKASKTVNANKLWDLELTGEKVTIAVLDTGVYEHPCFENRI
KKFRDFVNNGKKTYDDNGHGTHVAGCIASKQNEDFGGVAPKAEIVGVKVLNKLGSGRLSNVLDGIQWCIENKENLNLQIM
NLSLGFPTQESYRDDPLCQMVEKAWEAGIVVCAAAGNSGPDNETINSPGIHPQIITVGASNDRNADYPEDNIIADFSSRG
PTRDGHKKPDVVAPGVDIVSLRSPSSFLDKVEPGSRIGEDRFIFSGTSMATPICVGAIAQLLHYNKDLEPDEIKSLLMGN
AREIPGVNRESQGTGIIDSKEIQSNIREFSDS
>Mature_432_residues
MIRDSAWIPNYNYKLCPKLKNELYENYKPSKMVPTVFYNSYKQIKKKIRKFPVIVQVKFTKDNLTIMDQVASLAQCRIKK
KYYTIFSFSTDLTEVQLKKLLADDKVIKVWYDGEVTGLLEKASKTVNANKLWDLELTGEKVTIAVLDTGVYEHPCFENRI
KKFRDFVNNGKKTYDDNGHGTHVAGCIASKQNEDFGGVAPKAEIVGVKVLNKLGSGRLSNVLDGIQWCIENKENLNLQIM
NLSLGFPTQESYRDDPLCQMVEKAWEAGIVVCAAAGNSGPDNETINSPGIHPQIITVGASNDRNADYPEDNIIADFSSRG
PTRDGHKKPDVVAPGVDIVSLRSPSSFLDKVEPGSRIGEDRFIFSGTSMATPICVGAIAQLLHYNKDLEPDEIKSLLMGN
AREIPGVNRESQGTGIIDSKEIQSNIREFSDS

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=313, Percent_Identity=34.8242811501597, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI186972143, Length=336, Percent_Identity=27.6785714285714, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17533391, Length=260, Percent_Identity=28.0769230769231, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319893, Length=271, Percent_Identity=38.0073800738007, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320775, Length=268, Percent_Identity=35.8208955223881, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324576, Length=265, Percent_Identity=34.7169811320755, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550681, Length=300, Percent_Identity=33.6666666666667, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653270, Length=278, Percent_Identity=27.3381294964029, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137360, Length=278, Percent_Identity=27.3381294964029, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 48094; Mature: 48094

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRDSAWIPNYNYKLCPKLKNELYENYKPSKMVPTVFYNSYKQIKKKIRKFPVIVQVKFT
CCCCCCCCCCCCCEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEE
KDNLTIMDQVASLAQCRIKKKYYTIFSFSTDLTEVQLKKLLADDKVIKVWYDGEVTGLLE
CCCCHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCHHHHHH
KASKTVNANKLWDLELTGEKVTIAVLDTGVYEHPCFENRIKKFRDFVNNGKKTYDDNGHG
HHHHCCCCCEEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCC
THVAGCIASKQNEDFGGVAPKAEIVGVKVLNKLGSGRLSNVLDGIQWCIENKENLNLQIM
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEE
NLSLGFPTQESYRDDPLCQMVEKAWEAGIVVCAAAGNSGPDNETINSPGIHPQIITVGAS
EEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECC
NDRNADYPEDNIIADFSSRGPTRDGHKKPDVVAPGVDIVSLRSPSSFLDKVEPGSRIGED
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCEECCCCCEEEECCCHHHHHHCCCCCCCCCC
RFIFSGTSMATPICVGAIAQLLHYNKDLEPDEIKSLLMGNAREIPGVNRESQGTGIIDSK
CEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
EIQSNIREFSDS
HHHHHHHHHCCC
>Mature Secondary Structure
MIRDSAWIPNYNYKLCPKLKNELYENYKPSKMVPTVFYNSYKQIKKKIRKFPVIVQVKFT
CCCCCCCCCCCCCEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEE
KDNLTIMDQVASLAQCRIKKKYYTIFSFSTDLTEVQLKKLLADDKVIKVWYDGEVTGLLE
CCCCHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCHHHHHH
KASKTVNANKLWDLELTGEKVTIAVLDTGVYEHPCFENRIKKFRDFVNNGKKTYDDNGHG
HHHHCCCCCEEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCC
THVAGCIASKQNEDFGGVAPKAEIVGVKVLNKLGSGRLSNVLDGIQWCIENKENLNLQIM
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEE
NLSLGFPTQESYRDDPLCQMVEKAWEAGIVVCAAAGNSGPDNETINSPGIHPQIITVGAS
EEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECC
NDRNADYPEDNIIADFSSRGPTRDGHKKPDVVAPGVDIVSLRSPSSFLDKVEPGSRIGED
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCEECCCCCEEEECCCHHHHHHCCCCCCCCCC
RFIFSGTSMATPICVGAIAQLLHYNKDLEPDEIKSLLMGNAREIPGVNRESQGTGIIDSK
CEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
EIQSNIREFSDS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]