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The map label for this gene is murE [H]

Identifier: 188586220

GI number: 188586220

Start: 1679118

End: 1680740

Strand: Direct

Name: murE [H]

Synonym: Nther_1603

Alternate gene names: 188586220

Gene position: 1679118-1680740 (Clockwise)

Preceding gene: 188586219

Following gene: 188586225

Centisome position: 53.04

GC content: 34.75

Gene sequence:

>1623_bases
ATGCCTGATAATGTTAGTTTAAGGGCTTTAATAGAAAATTATCCTATAAAAAGCATAGTCATGCCTGGTAATTCTGGAAA
TATTAAGCAAGCCCTAGACGATATCAAAATATCCGGTTTAAATTATCATTCCCAAAATGTTTCCCAAAATGAGTTATTTT
TTTGTTTAACGGGCCATCAATACGATGGCCATGCTTTTGCAGAAAAAGCCTACAACAAAGGGGCAGTTGCATTAATAGTT
GAGCGAGAAATCCCCCATATTCCTCTCTTGCAAGTAGTAGTTGAAAATTCAAGACGAGCCCTTTCTCATCTTAGTGCTAA
GTTTTATCAACATCCTTCCAAGTCTATGAATATGATAGGAATTACTTCTACTAATGGTAAAACAACTACCACTTACATGA
TTGATCAAATTCTTAGCAGCAACAAACCCACGGGCTTATTGGGAACTGTTATAGTAAAATTAGGAGAAGAGACAAAGGAA
TCGGATTTAACCACCCCTGAATCTTTAGATCTCCAAAGATACTTAGCTTCTATGAAAAGAAAAAATATCAATTATTGTAC
TATGGAAGTATCTTCTTCAGGATTAGAACTGGAACGGGTTTCTGCCGTGGACTATGATATCGCTATAATTAATAACATCT
CGAGGGACCATATTGACCAACACGGCTCTTTCGAAAACTATTTTGCAGCTAAACAGCGTTTAGTAGAAGAAATTCAGGAA
GATAAAAGGGCTGTTATTAATGTTGATTGTGAAAATGCCTGTAAAATGATTAATTACACTAAAGCGAAAGTTATAACTTA
TAGTGTAAAAGGCATAGATGCTACAATTGGAATTGACAATCTGGATTTATCATCAGGAAGTGCCAGTTTTGATCTAGTTA
TCAATAGAGGTTTCTCTACTCTATCGGGCAAATGGATAAAACCAGGTCGATTTAAAATCAAATTAAAAGTATTGGGCCTT
CATAATGTCTATAATGCTACTTCTGCTGCTATATCGACATTATTATTGGATGAACCTATAACTCGGATACAGTCAGAGCT
GGCTAAATTTTCAGGTGTAGAACGCAGATTCCAACTGATATTTGACAAAAAATTCAAAATTATAGATGATCACTTTGCCA
ATCCCGGAAATATTGAAGTCACCATGGAAACCCTAGGAAAGATGGATTTTAATAACCTAAAAATTTTATATGCCATTAGA
GGTGGTAGAGGTAAGACTGTTAATGAGGAAAATACTAAGGCTTTAATTAAATGGCTAAAAAAATTAAACCTTCAAGAAGT
TTATGTAACATCAAGTGATGAAATAGTGGGAATTGAGAATAAAGTTACGATGACAGAAAGAGATACCACCCTTTCTCTTT
TGAAATCAGCTGGAATAAGGGTTAGATATTTCTCTCAACTAAGATCAGCTATTAAGTCTGTTATTATTGAAAGACATTTA
CAAACTGGCGATGTTCTCTTACTAGCTGGATGTCAAGGTATGGATCCGGGTGCTAGACTAGTTTTAAATTTAATATACAA
TAGACTAACCAAAAATCAGCAAAAATACTGGGGTGACGAGATCTTAGCGCCTTTAAAGCATAGGGTCGCAGGTATGAATT
GTAATATTTCTAAGCCGATATAA

Upstream 100 bases:

>100_bases
TCCAGCAGGGCTTTTTGACCCTGAAACCTGGAGAACTTTTATTCATAGGTTGAGATCTTTTTAAACTCAACGAAGATTAT
TATACTCTGAGGTGAATAAT

Downstream 100 bases:

>100_bases
TTTTTTTAAAATTTTTGATTCAATAAATCCCTTGACTATATTTATTGCATGAGTTATACTAATAAATGTCGCGGCGGGAT
AAAATAATTCTGCGGCAAGA

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MPDNVSLRALIENYPIKSIVMPGNSGNIKQALDDIKISGLNYHSQNVSQNELFFCLTGHQYDGHAFAEKAYNKGAVALIV
EREIPHIPLLQVVVENSRRALSHLSAKFYQHPSKSMNMIGITSTNGKTTTTYMIDQILSSNKPTGLLGTVIVKLGEETKE
SDLTTPESLDLQRYLASMKRKNINYCTMEVSSSGLELERVSAVDYDIAIINNISRDHIDQHGSFENYFAAKQRLVEEIQE
DKRAVINVDCENACKMINYTKAKVITYSVKGIDATIGIDNLDLSSGSASFDLVINRGFSTLSGKWIKPGRFKIKLKVLGL
HNVYNATSAAISTLLLDEPITRIQSELAKFSGVERRFQLIFDKKFKIIDDHFANPGNIEVTMETLGKMDFNNLKILYAIR
GGRGKTVNEENTKALIKWLKKLNLQEVYVTSSDEIVGIENKVTMTERDTTLSLLKSAGIRVRYFSQLRSAIKSVIIERHL
QTGDVLLLAGCQGMDPGARLVLNLIYNRLTKNQQKYWGDEILAPLKHRVAGMNCNISKPI

Sequences:

>Translated_540_residues
MPDNVSLRALIENYPIKSIVMPGNSGNIKQALDDIKISGLNYHSQNVSQNELFFCLTGHQYDGHAFAEKAYNKGAVALIV
EREIPHIPLLQVVVENSRRALSHLSAKFYQHPSKSMNMIGITSTNGKTTTTYMIDQILSSNKPTGLLGTVIVKLGEETKE
SDLTTPESLDLQRYLASMKRKNINYCTMEVSSSGLELERVSAVDYDIAIINNISRDHIDQHGSFENYFAAKQRLVEEIQE
DKRAVINVDCENACKMINYTKAKVITYSVKGIDATIGIDNLDLSSGSASFDLVINRGFSTLSGKWIKPGRFKIKLKVLGL
HNVYNATSAAISTLLLDEPITRIQSELAKFSGVERRFQLIFDKKFKIIDDHFANPGNIEVTMETLGKMDFNNLKILYAIR
GGRGKTVNEENTKALIKWLKKLNLQEVYVTSSDEIVGIENKVTMTERDTTLSLLKSAGIRVRYFSQLRSAIKSVIIERHL
QTGDVLLLAGCQGMDPGARLVLNLIYNRLTKNQQKYWGDEILAPLKHRVAGMNCNISKPI
>Mature_539_residues
PDNVSLRALIENYPIKSIVMPGNSGNIKQALDDIKISGLNYHSQNVSQNELFFCLTGHQYDGHAFAEKAYNKGAVALIVE
REIPHIPLLQVVVENSRRALSHLSAKFYQHPSKSMNMIGITSTNGKTTTTYMIDQILSSNKPTGLLGTVIVKLGEETKES
DLTTPESLDLQRYLASMKRKNINYCTMEVSSSGLELERVSAVDYDIAIINNISRDHIDQHGSFENYFAAKQRLVEEIQED
KRAVINVDCENACKMINYTKAKVITYSVKGIDATIGIDNLDLSSGSASFDLVINRGFSTLSGKWIKPGRFKIKLKVLGLH
NVYNATSAAISTLLLDEPITRIQSELAKFSGVERRFQLIFDKKFKIIDDHFANPGNIEVTMETLGKMDFNNLKILYAIRG
GRGKTVNEENTKALIKWLKKLNLQEVYVTSSDEIVGIENKVTMTERDTTLSLLKSAGIRVRYFSQLRSAIKSVIIERHLQ
TGDVLLLAGCQGMDPGARLVLNLIYNRLTKNQQKYWGDEILAPLKHRVAGMNCNISKPI

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=219, Percent_Identity=35.1598173515982, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786274, Length=364, Percent_Identity=24.1758241758242, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 60412; Mature: 60281

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDNVSLRALIENYPIKSIVMPGNSGNIKQALDDIKISGLNYHSQNVSQNELFFCLTGHQ
CCCCCEEHHHHHCCCCCEEEECCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEEEEECCC
YDGHAFAEKAYNKGAVALIVEREIPHIPLLQVVVENSRRALSHLSAKFYQHPSKSMNMIG
CCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ITSTNGKTTTTYMIDQILSSNKPTGLLGTVIVKLGEETKESDLTTPESLDLQRYLASMKR
EECCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KNINYCTMEVSSSGLELERVSAVDYDIAIINNISRDHIDQHGSFENYFAAKQRLVEEIQE
CCCCEEEEEECCCCCEEEEEECCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
DKRAVINVDCENACKMINYTKAKVITYSVKGIDATIGIDNLDLSSGSASFDLVINRGFST
CCCEEEEECHHHHHHHHCCEEEEEEEEEECCCEEEECCCCCCCCCCCCEEEEEEECCCCC
LSGKWIKPGRFKIKLKVLGLHNVYNATSAAISTLLLDEPITRIQSELAKFSGVERRFQLI
CCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FDKKFKIIDDHFANPGNIEVTMETLGKMDFNNLKILYAIRGGRGKTVNEENTKALIKWLK
HCCCCEEEHHCCCCCCCEEEEHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHH
KLNLQEVYVTSSDEIVGIENKVTMTERDTTLSLLKSAGIRVRYFSQLRSAIKSVIIERHL
HCCCEEEEEECCCCEEECCCEEEEECCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHC
QTGDVLLLAGCQGMDPGARLVLNLIYNRLTKNQQKYWGDEILAPLKHRVAGMNCNISKPI
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PDNVSLRALIENYPIKSIVMPGNSGNIKQALDDIKISGLNYHSQNVSQNELFFCLTGHQ
CCCCEEHHHHHCCCCCEEEECCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEEEEECCC
YDGHAFAEKAYNKGAVALIVEREIPHIPLLQVVVENSRRALSHLSAKFYQHPSKSMNMIG
CCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ITSTNGKTTTTYMIDQILSSNKPTGLLGTVIVKLGEETKESDLTTPESLDLQRYLASMKR
EECCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KNINYCTMEVSSSGLELERVSAVDYDIAIINNISRDHIDQHGSFENYFAAKQRLVEEIQE
CCCCEEEEEECCCCCEEEEEECCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
DKRAVINVDCENACKMINYTKAKVITYSVKGIDATIGIDNLDLSSGSASFDLVINRGFST
CCCEEEEECHHHHHHHHCCEEEEEEEEEECCCEEEECCCCCCCCCCCCEEEEEEECCCCC
LSGKWIKPGRFKIKLKVLGLHNVYNATSAAISTLLLDEPITRIQSELAKFSGVERRFQLI
CCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FDKKFKIIDDHFANPGNIEVTMETLGKMDFNNLKILYAIRGGRGKTVNEENTKALIKWLK
HCCCCEEEHHCCCCCCCEEEEHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHH
KLNLQEVYVTSSDEIVGIENKVTMTERDTTLSLLKSAGIRVRYFSQLRSAIKSVIIERHL
HCCCEEEEEECCCCEEECCCEEEEECCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHC
QTGDVLLLAGCQGMDPGARLVLNLIYNRLTKNQQKYWGDEILAPLKHRVAGMNCNISKPI
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436954; 9384377; 8289242 [H]