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The map label for this gene is truB [H]

Identifier: 188586073

GI number: 188586073

Start: 1514664

End: 1515563

Strand: Direct

Name: truB [H]

Synonym: Nther_1447

Alternate gene names: 188586073

Gene position: 1514664-1515563 (Clockwise)

Preceding gene: 188586072

Following gene: 188586074

Centisome position: 47.85

GC content: 35.11

Gene sequence:

>900_bases
TTGCTAGGATTCCTTACTGTTTGTAAAACCCCGGGCTATACTTCTCATGATGTAGTTCAATATTTAAGGAAAAATTATCA
AGTAAAAAAAGTAGGGCATTTGGGTACTTTAGATCCAGGAGCTGTAGGTGTGCTACCTCTTGCCTTGGGGAAAGCTACGC
GCTTTTCTCAATATATATTATCCCAGGACAAGAAGTATAGATTTGAGTTAACTTTAGGTGAAGCTACTGATACTTTGGAC
GCCCAAGGTAAAACAATTGAAAAAGATGAAGTCCAGAATACTCATATTGAAAATTTAAAAAACAAGTATCAAGAGCTTAT
CGGAAATATAAATCAGGTTCCACCAATGTATTCTGCAATTAAACAAAAAGGCAAAAAACTTTATGAACTAGCTCGAGAAG
GGAAAGAGGTTGAACGAACCCCTAGACAAGTCACGATTCACTCTTTAAACTTAGTCGATATTTATGAACACCAGGGAAAA
CATAGATTTCTTTTTGATGTTCATTGTTCAAAGGGAACTTACATTAGAACTTTAGCAGAAGACTTGGCTAAGCTGTCAGG
AACCGTGGGGTATATGTCTTTTTTAATCAGAACAGCTACAGGCATATTTAATTTAGATGATGCCATATTTCCTCAAGAGC
TCCCTGAACAAAAAACTGATCTGGGCATTCATTTATTTCCTGTGGATTATCCCTTGTCAGATATCCCCTCTCTGGTTTTA
AAAGAATCTCAGCAATTGAAACGGTTTAAAAATGGAAACTTTGTTAATACTGCAGTTATAGCTGAATCAGGAGAACTTTA
TAGGATATATGAAGAAGATAGCTCAGGTGATAATTTATTTTTAGGATTAGGTAAAATCACGTCCAGTAATACTTTAAAAC
CTGAAAAAATTATAGCTTAA

Upstream 100 bases:

>100_bases
AGAGATGGATCCAAATAAATCAGTAGAATTAGTAATTTCTAAGCTAAAAACCGAGCTAGAGCTTTGAAAATAAAAAAAAG
TGTTTAAGGGGAGATTACTT

Downstream 100 bases:

>100_bases
TAGGTGATAATTGTGAAATTAATAAGCGACTTTAACTTAACTAAAGATAATAAAACTAAGAAATTATGGTTAAGCCTGGG
TAATTTTGATGGAGTGCATA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLGFLTVCKTPGYTSHDVVQYLRKNYQVKKVGHLGTLDPGAVGVLPLALGKATRFSQYILSQDKKYRFELTLGEATDTLD
AQGKTIEKDEVQNTHIENLKNKYQELIGNINQVPPMYSAIKQKGKKLYELAREGKEVERTPRQVTIHSLNLVDIYEHQGK
HRFLFDVHCSKGTYIRTLAEDLAKLSGTVGYMSFLIRTATGIFNLDDAIFPQELPEQKTDLGIHLFPVDYPLSDIPSLVL
KESQQLKRFKNGNFVNTAVIAESGELYRIYEEDSSGDNLFLGLGKITSSNTLKPEKIIA

Sequences:

>Translated_299_residues
MLGFLTVCKTPGYTSHDVVQYLRKNYQVKKVGHLGTLDPGAVGVLPLALGKATRFSQYILSQDKKYRFELTLGEATDTLD
AQGKTIEKDEVQNTHIENLKNKYQELIGNINQVPPMYSAIKQKGKKLYELAREGKEVERTPRQVTIHSLNLVDIYEHQGK
HRFLFDVHCSKGTYIRTLAEDLAKLSGTVGYMSFLIRTATGIFNLDDAIFPQELPEQKTDLGIHLFPVDYPLSDIPSLVL
KESQQLKRFKNGNFVNTAVIAESGELYRIYEEDSSGDNLFLGLGKITSSNTLKPEKIIA
>Mature_299_residues
MLGFLTVCKTPGYTSHDVVQYLRKNYQVKKVGHLGTLDPGAVGVLPLALGKATRFSQYILSQDKKYRFELTLGEATDTLD
AQGKTIEKDEVQNTHIENLKNKYQELIGNINQVPPMYSAIKQKGKKLYELAREGKEVERTPRQVTIHSLNLVDIYEHQGK
HRFLFDVHCSKGTYIRTLAEDLAKLSGTVGYMSFLIRTATGIFNLDDAIFPQELPEQKTDLGIHLFPVDYPLSDIPSLVL
KESQQLKRFKNGNFVNTAVIAESGELYRIYEEDSSGDNLFLGLGKITSSNTLKPEKIIA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=195, Percent_Identity=36.4102564102564, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI215599015, Length=252, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4503337, Length=252, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI2367200, Length=308, Percent_Identity=37.6623376623377, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17553978, Length=202, Percent_Identity=26.7326732673267, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324037, Length=133, Percent_Identity=39.8496240601504, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=205, Percent_Identity=28.2926829268293, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281364189, Length=208, Percent_Identity=27.8846153846154, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364187, Length=208, Percent_Identity=27.8846153846154, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364185, Length=208, Percent_Identity=27.8846153846154, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364183, Length=208, Percent_Identity=27.8846153846154, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI62471759, Length=208, Percent_Identity=27.8846153846154, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17975520, Length=208, Percent_Identity=27.8846153846154, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33643; Mature: 33643

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGFLTVCKTPGYTSHDVVQYLRKNYQVKKVGHLGTLDPGAVGVLPLALGKATRFSQYIL
CCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
SQDKKYRFELTLGEATDTLDAQGKTIEKDEVQNTHIENLKNKYQELIGNINQVPPMYSAI
CCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
KQKGKKLYELAREGKEVERTPRQVTIHSLNLVDIYEHQGKHRFLFDVHCSKGTYIRTLAE
HHHHHHHHHHHHCCCHHHCCCCEEEEEEEEEEEHHHCCCCEEEEEEEECCCCCHHHHHHH
DLAKLSGTVGYMSFLIRTATGIFNLDDAIFPQELPEQKTDLGIHLFPVDYPLSDIPSLVL
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHCCCEEEEECCCCHHHHHHHHH
KESQQLKRFKNGNFVNTAVIAESGELYRIYEEDSSGDNLFLGLGKITSSNTLKPEKIIA
HHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHCC
>Mature Secondary Structure
MLGFLTVCKTPGYTSHDVVQYLRKNYQVKKVGHLGTLDPGAVGVLPLALGKATRFSQYIL
CCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
SQDKKYRFELTLGEATDTLDAQGKTIEKDEVQNTHIENLKNKYQELIGNINQVPPMYSAI
CCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
KQKGKKLYELAREGKEVERTPRQVTIHSLNLVDIYEHQGKHRFLFDVHCSKGTYIRTLAE
HHHHHHHHHHHHCCCHHHCCCCEEEEEEEEEEEHHHCCCCEEEEEEEECCCCCHHHHHHH
DLAKLSGTVGYMSFLIRTATGIFNLDDAIFPQELPEQKTDLGIHLFPVDYPLSDIPSLVL
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHCCCEEEEECCCCHHHHHHHHH
KESQQLKRFKNGNFVNTAVIAESGELYRIYEEDSSGDNLFLGLGKITSSNTLKPEKIIA
HHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA