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The map label for this gene is rlmN

Identifier: 188585964

GI number: 188585964

Start: 1411875

End: 1412930

Strand: Direct

Name: rlmN

Synonym: Nther_1338

Alternate gene names: 188585964

Gene position: 1411875-1412930 (Clockwise)

Preceding gene: 188585963

Following gene: 188585965

Centisome position: 44.6

GC content: 35.8

Gene sequence:

>1056_bases
ATGATTAATCACAAGCAATCTTTAAAAGATCTTACCTTGAATGAATTGCAGGAATATTTTAGTCGTAAAGGGTGGCAACA
ATTTCGGGCGAAACAAATCTTTGACTGGATGTATATTCAACAAGTTGACTCCATTGAAGTGATGTCTAATATCCCTAAGA
AACTGCGCCAAGAATTGATGGAAAACTGCACCATTAATGATTTAGAGTTAGATAGTAATAATATATATACATCACCAACA
GATGGAACAATTAAATTTTTAAGTGTTCTCAAAGACGGTATAGGTGTAGAAACTACTATCATGAAATATGATTATGGTAA
TACTGTCTGTATTTCTTCTCAAGCGGGATGCAATATGAATTGTGTGTTTTGCGCATCGACTACTGGGGGTAAAGAAAGGG
ATTTGAGCCCAGGAGAAATGATTGATCAAGTACTTATGGCAAATAAAGTGCTCCCGGGTTCGGAATCGATAAATAATATT
GTTGTTATGGGAAGTGGAGAACCTTTAGAAAATTATCAACATCTTATCAAGTTTTTAAAAATAGTCAATGACGGTAAAGG
TTTAAATATTGGTATGAGGCATATTACTGTATCTACTTGTGGTTTAGTACCTGAAATCTATAATCTTGCTGAAGAAGAGT
TGCAGTTAAACTTGGCAATTTCACTCCATGCTCCCAATGACGAATTGAGAAATAAACTGATTCCTTTAAATAAAATATAT
CCAATTCACGAACTGCTAGAAGCCTGTCAAGTGTATTTTCAAAAAACTGGGAGACGGATAACTTTTGAATACGTACTGAT
TAAGGACTTCAATGATTCAATTGATTTGGCAAAAGAACTTTCCGAAACTTTAACAGCATTGAAGATGCCCGTACATGTTA
ATCTGATACCCTTTAACCCAGTTGAAGAAACTAAATTTACGGCACCTCCGAGTTCCAGAATCTCGGATTTTAAAAATAAT
TTACAGTCGAATAATATTGGCGTTACTGTACGCAAAGAGCGCGGGGTCGATGTTGACGGCGCTTGCGGGCAGTTAAGATC
AAAGGTAATGAGGTGA

Upstream 100 bases:

>100_bases
ATCTAATTCCATTACAACTCCCTGCACTGTTAGTTGCTATGTGTCCTTTACTCTGATATACTTTGGCATGAATAAGTAAT
GAAGATGGGGATGATGCAAT

Downstream 100 bases:

>100_bases
AAAAATGCAGTTTTTCGGGATGAGTGATGTAGGGTGCTTACGTGAAAAAAACGAGGATAATTTTATTCAAGGCATCAAAA
CAGGAGACTTAGTAATAACA

Product: radical SAM enzyme, Cfr family

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MINHKQSLKDLTLNELQEYFSRKGWQQFRAKQIFDWMYIQQVDSIEVMSNIPKKLRQELMENCTINDLELDSNNIYTSPT
DGTIKFLSVLKDGIGVETTIMKYDYGNTVCISSQAGCNMNCVFCASTTGGKERDLSPGEMIDQVLMANKVLPGSESINNI
VVMGSGEPLENYQHLIKFLKIVNDGKGLNIGMRHITVSTCGLVPEIYNLAEEELQLNLAISLHAPNDELRNKLIPLNKIY
PIHELLEACQVYFQKTGRRITFEYVLIKDFNDSIDLAKELSETLTALKMPVHVNLIPFNPVEETKFTAPPSSRISDFKNN
LQSNNIGVTVRKERGVDVDGACGQLRSKVMR

Sequences:

>Translated_351_residues
MINHKQSLKDLTLNELQEYFSRKGWQQFRAKQIFDWMYIQQVDSIEVMSNIPKKLRQELMENCTINDLELDSNNIYTSPT
DGTIKFLSVLKDGIGVETTIMKYDYGNTVCISSQAGCNMNCVFCASTTGGKERDLSPGEMIDQVLMANKVLPGSESINNI
VVMGSGEPLENYQHLIKFLKIVNDGKGLNIGMRHITVSTCGLVPEIYNLAEEELQLNLAISLHAPNDELRNKLIPLNKIY
PIHELLEACQVYFQKTGRRITFEYVLIKDFNDSIDLAKELSETLTALKMPVHVNLIPFNPVEETKFTAPPSSRISDFKNN
LQSNNIGVTVRKERGVDVDGACGQLRSKVMR
>Mature_351_residues
MINHKQSLKDLTLNELQEYFSRKGWQQFRAKQIFDWMYIQQVDSIEVMSNIPKKLRQELMENCTINDLELDSNNIYTSPT
DGTIKFLSVLKDGIGVETTIMKYDYGNTVCISSQAGCNMNCVFCASTTGGKERDLSPGEMIDQVLMANKVLPGSESINNI
VVMGSGEPLENYQHLIKFLKIVNDGKGLNIGMRHITVSTCGLVPEIYNLAEEELQLNLAISLHAPNDELRNKLIPLNKIY
PIHELLEACQVYFQKTGRRITFEYVLIKDFNDSIDLAKELSETLTALKMPVHVNLIPFNPVEETKFTAPPSSRISDFKNN
LQSNNIGVTVRKERGVDVDGACGQLRSKVMR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=356, Percent_Identity=37.3595505617978, Blast_Score=227, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_NATTJ (B2A2K6)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917509.1
- ProteinModelPortal:   B2A2K6
- GeneID:   6316556
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1338
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39632; Mature: 39632

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINHKQSLKDLTLNELQEYFSRKGWQQFRAKQIFDWMYIQQVDSIEVMSNIPKKLRQELM
CCCCHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENCTINDLELDSNNIYTSPTDGTIKFLSVLKDGIGVETTIMKYDYGNTVCISSQAGCNMN
HCCCCCEEEECCCCEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCE
CVFCASTTGGKERDLSPGEMIDQVLMANKVLPGSESINNIVVMGSGEPLENYQHLIKFLK
EEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
IVNDGKGLNIGMRHITVSTCGLVPEIYNLAEEELQLNLAISLHAPNDELRNKLIPLNKIY
HHCCCCCCEEEEEEEEEHHHHCHHHHHHHHHHHEEEEEEEEEECCCHHHHHHCCCCCCCC
PIHELLEACQVYFQKTGRRITFEYVLIKDFNDSIDLAKELSETLTALKMPVHVNLIPFNP
CHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCC
VEETKFTAPPSSRISDFKNNLQSNNIGVTVRKERGVDVDGACGQLRSKVMR
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MINHKQSLKDLTLNELQEYFSRKGWQQFRAKQIFDWMYIQQVDSIEVMSNIPKKLRQELM
CCCCHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENCTINDLELDSNNIYTSPTDGTIKFLSVLKDGIGVETTIMKYDYGNTVCISSQAGCNMN
HCCCCCEEEECCCCEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCE
CVFCASTTGGKERDLSPGEMIDQVLMANKVLPGSESINNIVVMGSGEPLENYQHLIKFLK
EEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
IVNDGKGLNIGMRHITVSTCGLVPEIYNLAEEELQLNLAISLHAPNDELRNKLIPLNKIY
HHCCCCCCEEEEEEEEEHHHHCHHHHHHHHHHHEEEEEEEEEECCCHHHHHHCCCCCCCC
PIHELLEACQVYFQKTGRRITFEYVLIKDFNDSIDLAKELSETLTALKMPVHVNLIPFNP
CHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCC
VEETKFTAPPSSRISDFKNNLQSNNIGVTVRKERGVDVDGACGQLRSKVMR
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA