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The map label for this gene is rsmH

Identifier: 188585922

GI number: 188585922

Start: 1367300

End: 1368247

Strand: Direct

Name: rsmH

Synonym: Nther_1296

Alternate gene names: 188585922

Gene position: 1367300-1368247 (Clockwise)

Preceding gene: 188585921

Following gene: 188585923

Centisome position: 43.19

GC content: 36.39

Gene sequence:

>948_bases
ATGAATTTTAATCATGAACCGGTTATGATTGAAAAAACAATAGAATTATTAAAACCTGAGTCGGCTCAAGTTATTGTTGA
TGGAACCATGGGAGGAGCTGGCCACTCATTAAGAATTATTAATAGTTTACCTCCTGAAGGCATTCTAGTTGGTATTGACC
AAGATCCAGATGCTTATGAAGCGGGAAAATCTCTGTTAGAGGCTTATGGAAATAAAGCTAAAGTTTTTAATGACAATTTT
GTAAATATAAAACATATCTGTGAAAGAATTGGTATTCATTATGTGGACGGAATTTTACTAGACTTAGGTGTTTCTAGTTA
TCAACTTGATAATAAAGACAGGGGTTTCACTTATCAAGAAAATGCACCTTTAGATATGAGAATGGATAAAAGCCAAAAGA
TAACAGCTAGCGAAGTAGTTAACACTTATTCAGAGCAAAAATTAGCCGAGATAATCCGAGAATATGGGGAGGAAAAATGG
GCACAGAGAATAGCACAATTTATTGTTCAGGCCCGTCAAGATCAGGAGATAAATACAACACAAGAGTTAGTAAAAGTAAT
TAAGGCTGCAATACCTAAAGGAGCTAGAAAATCAGGGCCTCATCCGGCAAAAAGGACATTTCAAGCCCTTAGAATACATG
TTAACGGAGAGCTTGATAGTTTAAGAACGGCCATCCAAGAAGGGATTCGGTTACTAAGGGGTAAAGGTCGATTTGTAGTT
ATTACATTTCATTCTCTAGAAGACAGGATAGTCAAGCAAGAATTTAAAAAATTAGCTCAAACCTGCGTTTGTCCTACTAA
ATTGCCGATATGCCAGTGTAAGGGAGAAGCTACAGTTAAATTACTAACAAAAAAACCTCTACTGCCAAGTCAAGGGGAAA
TAGAAATTAATCCTAGGGCAAGAAGTGCAAAATTAAGAGCAGTGGAGCGATTGGGTTCTAACAATTGA

Upstream 100 bases:

>100_bases
AGCAACAAGCTGAAAGTTCCTTTGAAAATATTGCCGAAGAGATTGTGGATTTTGATATATAGCCTGACATTAAAGGATAA
AACAAGAAAGGTGATATACA

Downstream 100 bases:

>100_bases
AAGGGGTGAATATATTGTATAAAAATCAAGTGACTGCCGAGGCAATACCTAAAACTCATAGACAAACCGCACCCCAAAGA
AGTCCCCAAGAACGTAAGAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNFNHEPVMIEKTIELLKPESAQVIVDGTMGGAGHSLRIINSLPPEGILVGIDQDPDAYEAGKSLLEAYGNKAKVFNDNF
VNIKHICERIGIHYVDGILLDLGVSSYQLDNKDRGFTYQENAPLDMRMDKSQKITASEVVNTYSEQKLAEIIREYGEEKW
AQRIAQFIVQARQDQEINTTQELVKVIKAAIPKGARKSGPHPAKRTFQALRIHVNGELDSLRTAIQEGIRLLRGKGRFVV
ITFHSLEDRIVKQEFKKLAQTCVCPTKLPICQCKGEATVKLLTKKPLLPSQGEIEINPRARSAKLRAVERLGSNN

Sequences:

>Translated_315_residues
MNFNHEPVMIEKTIELLKPESAQVIVDGTMGGAGHSLRIINSLPPEGILVGIDQDPDAYEAGKSLLEAYGNKAKVFNDNF
VNIKHICERIGIHYVDGILLDLGVSSYQLDNKDRGFTYQENAPLDMRMDKSQKITASEVVNTYSEQKLAEIIREYGEEKW
AQRIAQFIVQARQDQEINTTQELVKVIKAAIPKGARKSGPHPAKRTFQALRIHVNGELDSLRTAIQEGIRLLRGKGRFVV
ITFHSLEDRIVKQEFKKLAQTCVCPTKLPICQCKGEATVKLLTKKPLLPSQGEIEINPRARSAKLRAVERLGSNN
>Mature_315_residues
MNFNHEPVMIEKTIELLKPESAQVIVDGTMGGAGHSLRIINSLPPEGILVGIDQDPDAYEAGKSLLEAYGNKAKVFNDNF
VNIKHICERIGIHYVDGILLDLGVSSYQLDNKDRGFTYQENAPLDMRMDKSQKITASEVVNTYSEQKLAEIIREYGEEKW
AQRIAQFIVQARQDQEINTTQELVKVIKAAIPKGARKSGPHPAKRTFQALRIHVNGELDSLRTAIQEGIRLLRGKGRFVV
ITFHSLEDRIVKQEFKKLAQTCVCPTKLPICQCKGEATVKLLTKKPLLPSQGEIEINPRARSAKLRAVERLGSNN

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=37.0919881305638, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=38.2978723404255, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=38.7820512820513, Blast_Score=220, Evalue=1e-58,
Organism=Drosophila melanogaster, GI62472493, Length=326, Percent_Identity=35.5828220858896, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28571637, Length=326, Percent_Identity=35.5828220858896, Blast_Score=173, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_NATTJ (B2A2G4)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917467.1
- ProteinModelPortal:   B2A2G4
- GeneID:   6315159
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1296
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35251; Mature: 35251

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: BINDING 53-53 BINDING 80-80 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNHEPVMIEKTIELLKPESAQVIVDGTMGGAGHSLRIINSLPPEGILVGIDQDPDAYE
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCHHH
AGKSLLEAYGNKAKVFNDNFVNIKHICERIGIHYVDGILLDLGVSSYQLDNKDRGFTYQE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHHCCCCCEECCCCCCCEEECC
NAPLDMRMDKSQKITASEVVNTYSEQKLAEIIREYGEEKWAQRIAQFIVQARQDQEINTT
CCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QELVKVIKAAIPKGARKSGPHPAKRTFQALRIHVNGELDSLRTAIQEGIRLLRGKGRFVV
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEE
ITFHSLEDRIVKQEFKKLAQTCVCPTKLPICQCKGEATVKLLTKKPLLPSQGEIEINPRA
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEEECCCCCCCCCCEEECCCH
RSAKLRAVERLGSNN
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFNHEPVMIEKTIELLKPESAQVIVDGTMGGAGHSLRIINSLPPEGILVGIDQDPDAYE
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCHHH
AGKSLLEAYGNKAKVFNDNFVNIKHICERIGIHYVDGILLDLGVSSYQLDNKDRGFTYQE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHHCCCCCEECCCCCCCEEECC
NAPLDMRMDKSQKITASEVVNTYSEQKLAEIIREYGEEKWAQRIAQFIVQARQDQEINTT
CCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QELVKVIKAAIPKGARKSGPHPAKRTFQALRIHVNGELDSLRTAIQEGIRLLRGKGRFVV
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEE
ITFHSLEDRIVKQEFKKLAQTCVCPTKLPICQCKGEATVKLLTKKPLLPSQGEIEINPRA
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEEECCCCCCCCCCEEECCCH
RSAKLRAVERLGSNN
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA