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The map label for this gene is ogt [H]

Identifier: 188585904

GI number: 188585904

Start: 1347647

End: 1348147

Strand: Direct

Name: ogt [H]

Synonym: Nther_1278

Alternate gene names: 188585904

Gene position: 1347647-1348147 (Clockwise)

Preceding gene: 188585903

Following gene: 188585905

Centisome position: 42.57

GC content: 37.13

Gene sequence:

>501_bases
ATGTTTAAAAAGATGCTTTATGAAACTAAATGGGGAACAATCACCTTTGGGCATAATCAGGGCATTGTAAATAGAGTGAT
TTTACCTGATAAGCAGGAAGAAACAGGTTTAGAAGAGGAACTATCCAGCTATCAAAATCAAAAACTAACAGCTCTAGAAA
GAGAACTGAACTTGTATTTTCAAGGAAGACTAGTGAATTTTTCAATTCCAGTTTCTTTGGAAGGTCTACCTACCTTTACT
AAACAGGTATTGGAAGTTACTAAAAGGGTCAATTGGGGAGAAGTGTGCACATATGGACAGATAGCTAAAATATTAAATAA
ACCAGGGGCAAGTAGAGCAGTCGGACAATCTTTAGGAAAAAATCCCTGCCCCCTATTGGTGCCGTGTCACCGAGTTATTG
GTAGAAATAATAGCCTGGGAGGTTTTTCGGGTATGATAGAGTTAAAAAAATGGTTTCTGAACCTAGAAGGTGTTAATTTG
AAGGTTTTGAAGGAGGTATAA

Upstream 100 bases:

>100_bases
CGTCTCAGGGTTTAATGTATTATATTTAGATCTTATGATATAAATCAAGTATTCTTTAATCGGATCAAGTATCCTATAAT
ACTATAGTCGGGGGGAGTTA

Downstream 100 bases:

>100_bases
ATATGGATCCAGTAGCTTTTGAAATTGGACCGATTTCAGTATACTGGTATGGCATTTTGATTACTTTAGGGATTTTAATC
GGCTTGTATTTAGCTATTAG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MFKKMLYETKWGTITFGHNQGIVNRVILPDKQEETGLEEELSSYQNQKLTALERELNLYFQGRLVNFSIPVSLEGLPTFT
KQVLEVTKRVNWGEVCTYGQIAKILNKPGASRAVGQSLGKNPCPLLVPCHRVIGRNNSLGGFSGMIELKKWFLNLEGVNL
KVLKEV

Sequences:

>Translated_166_residues
MFKKMLYETKWGTITFGHNQGIVNRVILPDKQEETGLEEELSSYQNQKLTALERELNLYFQGRLVNFSIPVSLEGLPTFT
KQVLEVTKRVNWGEVCTYGQIAKILNKPGASRAVGQSLGKNPCPLLVPCHRVIGRNNSLGGFSGMIELKKWFLNLEGVNL
KVLKEV
>Mature_166_residues
MFKKMLYETKWGTITFGHNQGIVNRVILPDKQEETGLEEELSSYQNQKLTALERELNLYFQGRLVNFSIPVSLEGLPTFT
KQVLEVTKRVNWGEVCTYGQIAKILNKPGASRAVGQSLGKNPCPLLVPCHRVIGRNNSLGGFSGMIELKKWFLNLEGVNL
KVLKEV

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=111, Percent_Identity=39.6396396396396, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1787596, Length=108, Percent_Identity=41.6666666666667, Blast_Score=93, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320001, Length=92, Percent_Identity=41.304347826087, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18708; Mature: 18708

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKMLYETKWGTITFGHNQGIVNRVILPDKQEETGLEEELSSYQNQKLTALERELNLYF
CCHHHHEECCCCEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHCEEE
QGRLVNFSIPVSLEGLPTFTKQVLEVTKRVNWGEVCTYGQIAKILNKPGASRAVGQSLGK
EEEEEEEECCEEECCCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCCHHHHHHHCC
NPCPLLVPCHRVIGRNNSLGGFSGMIELKKWFLNLEGVNLKVLKEV
CCCCEEEHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MFKKMLYETKWGTITFGHNQGIVNRVILPDKQEETGLEEELSSYQNQKLTALERELNLYF
CCHHHHEECCCCEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHCEEE
QGRLVNFSIPVSLEGLPTFTKQVLEVTKRVNWGEVCTYGQIAKILNKPGASRAVGQSLGK
EEEEEEEECCEEECCCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHCCCCCCHHHHHHHCC
NPCPLLVPCHRVIGRNNSLGGFSGMIELKKWFLNLEGVNLKVLKEV
CCCCEEEHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]