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The map label for this gene is dacF [H]

Identifier: 188585838

GI number: 188585838

Start: 1283702

End: 1284928

Strand: Reverse

Name: dacF [H]

Synonym: Nther_1212

Alternate gene names: 188585838

Gene position: 1284928-1283702 (Counterclockwise)

Preceding gene: 188585845

Following gene: 188585802

Centisome position: 40.59

GC content: 36.35

Gene sequence:

>1227_bases
GTGAAGTTTAACAATATCTACATTATTATACCCTTATTATTTTTAATGATTGTTAATCCCCTGTTATCACCATCAGTTTC
AGCCGAAGAATTTGACTTTGATGCAGAATCAGTTATATTAATCGAAGCTGAAACAGGGAGAACCATTTACGAAAATAATG
CCGAGAAAAGTTGGCCACCAGCTAGTTTGACCAAAGTAATGACTCTTGTAATTGGTTTAGAAGCTCTAAATGAAGGTGAG
CTAGAACTTGAAGAAACAACCAGAGTTAGTGCCAAGGCCAATCAAACGGGCGGCAGTCAGATGTTTCTCAGAGAAAATCA
AGAAGTTACCATTGAAGATCTCCTGACTGGAATCGCTGTAGTTTCTGCTAATGACGGTTGTGTTGCTCTAGCAGAACATA
TGGAAGGTTCAGTAAATAATTTTGTCAATCGCATGAATGAAAAGGCAGAAGAAATAGGATTAGAAAACACTAATTTTAAA
GACCCTCATGGTTTAGCTTCTGATGGGCAGTATATGAACGCTCGTGATGTAGCTCGACTATCCCAGTATGCCGTTAATGA
AACGCCTAAAATTTTAGAATTAGAAAGTATAACTGGATTTCAATTCAATATTGAAAGCACCCAATATAATAGAAATTTAA
TGTTACCTGACCACCCCCAAACCACAAGATATTCCTATGAAGGAACGGATGGTTTGAAAACTGGTCAAATCGAAGAAGCA
GGTTATAACTTTGTGGGTACTGCCCAACGCGACGATATGAGACTAATTGCAGTTGTTTTAGGTGCTGAAGAACAAACTGA
AAGATTTAATATTACTCGCCAACTTTTTGATCACGGATTCTCGGAGTATCAAAAAATAAAGTTATTTGATGAAGGTGACG
AATTGGAACAAGTAGATGTTAAAGATGGCTCAGAACGCCAAACAATGGCAATATCAGAAACTGAAGCAAATGTAACTACC
AAGTTTGACAATGATCAAGAACTTGAATTTGTATTTCAACCAAAAGATGAAGAACTTAGAGCCCCAATCCAAAAAGGTGA
ATATTTAGGTGAGTTATTAGTCATTGAAGACGGAAAAGAAATAGCTTCTATCCCCGGAATTGCTCAAGATGAAGTAGAAG
AACTTGGTTTTTTTGGTAGAATTGCTAGAACAATTAGTGATGCAATATCCAACACATTTGAGAGTATAAAAGATAGTATA
GTCGACTTTATTTTTGGCGATGATTAA

Upstream 100 bases:

>100_bases
AAAGTTTACATTTATTAATAGTGAAATGTAACAGCACCTTGTATAAGAGGAAAAACTAAGGCCAACAATATTATTACGCA
AAAACAAGGAGGAAAAGCCA

Downstream 100 bases:

>100_bases
ATTTATGTTGTAATATTTGTTAAATTACCAGCTTAAATTTGAGTTAAAAAGATATGCTCCCCTTAAGGTTGCAGTTAAAA
ACAAATCTGTACCTAAAGGG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKFNNIYIIIPLLFLMIVNPLLSPSVSAEEFDFDAESVILIEAETGRTIYENNAEKSWPPASLTKVMTLVIGLEALNEGE
LELEETTRVSAKANQTGGSQMFLRENQEVTIEDLLTGIAVVSANDGCVALAEHMEGSVNNFVNRMNEKAEEIGLENTNFK
DPHGLASDGQYMNARDVARLSQYAVNETPKILELESITGFQFNIESTQYNRNLMLPDHPQTTRYSYEGTDGLKTGQIEEA
GYNFVGTAQRDDMRLIAVVLGAEEQTERFNITRQLFDHGFSEYQKIKLFDEGDELEQVDVKDGSERQTMAISETEANVTT
KFDNDQELEFVFQPKDEELRAPIQKGEYLGELLVIEDGKEIASIPGIAQDEVEELGFFGRIARTISDAISNTFESIKDSI
VDFIFGDD

Sequences:

>Translated_408_residues
MKFNNIYIIIPLLFLMIVNPLLSPSVSAEEFDFDAESVILIEAETGRTIYENNAEKSWPPASLTKVMTLVIGLEALNEGE
LELEETTRVSAKANQTGGSQMFLRENQEVTIEDLLTGIAVVSANDGCVALAEHMEGSVNNFVNRMNEKAEEIGLENTNFK
DPHGLASDGQYMNARDVARLSQYAVNETPKILELESITGFQFNIESTQYNRNLMLPDHPQTTRYSYEGTDGLKTGQIEEA
GYNFVGTAQRDDMRLIAVVLGAEEQTERFNITRQLFDHGFSEYQKIKLFDEGDELEQVDVKDGSERQTMAISETEANVTT
KFDNDQELEFVFQPKDEELRAPIQKGEYLGELLVIEDGKEIASIPGIAQDEVEELGFFGRIARTISDAISNTFESIKDSI
VDFIFGDD
>Mature_408_residues
MKFNNIYIIIPLLFLMIVNPLLSPSVSAEEFDFDAESVILIEAETGRTIYENNAEKSWPPASLTKVMTLVIGLEALNEGE
LELEETTRVSAKANQTGGSQMFLRENQEVTIEDLLTGIAVVSANDGCVALAEHMEGSVNNFVNRMNEKAEEIGLENTNFK
DPHGLASDGQYMNARDVARLSQYAVNETPKILELESITGFQFNIESTQYNRNLMLPDHPQTTRYSYEGTDGLKTGQIEEA
GYNFVGTAQRDDMRLIAVVLGAEEQTERFNITRQLFDHGFSEYQKIKLFDEGDELEQVDVKDGSERQTMAISETEANVTT
KFDNDQELEFVFQPKDEELRAPIQKGEYLGELLVIEDGKEIASIPGIAQDEVEELGFFGRIARTISDAISNTFESIKDSI
VDFIFGDD

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=375, Percent_Identity=33.0666666666667, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1786851, Length=367, Percent_Identity=32.9700272479564, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082022, Length=374, Percent_Identity=30.2139037433155, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87082054, Length=145, Percent_Identity=31.7241379310345, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45684; Mature: 45684

Theoretical pI: Translated: 3.98; Mature: 3.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNNIYIIIPLLFLMIVNPLLSPSVSAEEFDFDAESVILIEAETGRTIYENNAEKSWPP
CCCCCEEEHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECCCCEEECCCCCCCCCC
ASLTKVMTLVIGLEALNEGELELEETTRVSAKANQTGGSQMFLRENQEVTIEDLLTGIAV
HHHHHHHHHHHHHHHCCCCCEEEHHHHEECCCCCCCCCCEEEEECCCCEEHHHHHHCEEE
VSANDGCVALAEHMEGSVNNFVNRMNEKAEEIGLENTNFKDPHGLASDGQYMNARDVARL
EECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SQYAVNETPKILELESITGFQFNIESTQYNRNLMLPDHPQTTRYSYEGTDGLKTGQIEEA
HHHHHCCCCCEEEEECCCCEEEEECCCCCCCEECCCCCCCCCEEEECCCCCCCCCCCHHC
GYNFVGTAQRDDMRLIAVVLGAEEQTERFNITRQLFDHGFSEYQKIKLFDEGDELEQVDV
CCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCCEEECC
KDGSERQTMAISETEANVTTKFDNDQELEFVFQPKDEELRAPIQKGEYLGELLVIEDGKE
CCCCCCCEEEEECCCCCEEEECCCCCCEEEEECCCCHHHHCCHHCCCCCCEEEEEECCCH
IASIPGIAQDEVEELGFFGRIARTISDAISNTFESIKDSIVDFIFGDD
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFNNIYIIIPLLFLMIVNPLLSPSVSAEEFDFDAESVILIEAETGRTIYENNAEKSWPP
CCCCCEEEHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECCCCEEECCCCCCCCCC
ASLTKVMTLVIGLEALNEGELELEETTRVSAKANQTGGSQMFLRENQEVTIEDLLTGIAV
HHHHHHHHHHHHHHHCCCCCEEEHHHHEECCCCCCCCCCEEEEECCCCEEHHHHHHCEEE
VSANDGCVALAEHMEGSVNNFVNRMNEKAEEIGLENTNFKDPHGLASDGQYMNARDVARL
EECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SQYAVNETPKILELESITGFQFNIESTQYNRNLMLPDHPQTTRYSYEGTDGLKTGQIEEA
HHHHHCCCCCEEEEECCCCEEEEECCCCCCCEECCCCCCCCCEEEECCCCCCCCCCCHHC
GYNFVGTAQRDDMRLIAVVLGAEEQTERFNITRQLFDHGFSEYQKIKLFDEGDELEQVDV
CCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCCEEECC
KDGSERQTMAISETEANVTTKFDNDQELEFVFQPKDEELRAPIQKGEYLGELLVIEDGKE
CCCCCCCEEEEECCCCCEEEECCCCCCEEEEECCCCHHHHCCHHCCCCCCEEEEEECCCH
IASIPGIAQDEVEELGFFGRIARTISDAISNTFESIKDSIVDFIFGDD
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]