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The map label for this gene is ppdK [H]

Identifier: 188585837

GI number: 188585837

Start: 1280884

End: 1283532

Strand: Direct

Name: ppdK [H]

Synonym: Nther_1211

Alternate gene names: 188585837

Gene position: 1280884-1283532 (Clockwise)

Preceding gene: 188585836

Following gene: 188585839

Centisome position: 40.46

GC content: 37.9

Gene sequence:

>2649_bases
ATGACTTCGATGGATAAACTAGTTTATAAGTTTTCAGAAGGCAGCAGAGATATGAAGCCTTTTTTAGGGGGAAAGGGTGC
CAATCTTGCCGAAATGACGAAAATCGGTCTACCGGTTCCACCAGGATTTACAGTTTCCACTGATGCTTGTAATGAATACT
TTAAAAATAATGGGATATTAACAGAACAATTGCGGGAGCAAATCTTCACTGCTCTTTCGGAAATTGAAAATGCAACTAAT
AAAAAATTTGGTGATCCTTCTAATCCTTTATTAGTATCAGTGCGCTCTGGAGCAGTAATTTCCATGCCAGGGATGATGGA
CACAGTTTTGAATCTAGGATTGAATGATGAAACAGTACAAGGTCTGGCCCGTCAAACTTCCAATGATAGATTTGCTTATG
ATTCCTACAGACGTTTTATTCAAATGTTCAGTGATGTAGTTATGGGAATAGGAAATTATATGTTTGAAAATGAAATGTCC
CGCTTAAAAGAAGAATATCAGGTAACTGAAGATACTCAACTTAACAGTCAGGCATTACAGGACCTGATCACAAGGTATAA
GCAGGTATATAACAGAGAAACCGGTGAGGAATTCCCCCAGGACCCAAAAGACCAATTGATTATGGCAGTCAAAGCGGTTT
TTAATTCCTGGAATAATCAAAGAGCCAAAGTTTATAGAGAAATTCATAATATCTCTCACGAACTAGGTACTGCTGTTAAC
GTACAGACCATGGTATTTGGAAATATGGGGAATGATAGTGGAACAGGTGTGGCTTTTACGCGAAACCCGTCAACTGGTGA
GAAAAATATATATGGAGAATTTCTACTAAATGCCCAAGGAGAAGATGTGGTAGCAGGGATTAGAACACCAAAAGCGATTA
AAGATCTGGAGGATGAATTTCCTGAAGTTTATAATCAGTTTCTAGAAGTCAGTCAACTTCTAGAAAACCATTATTGCGAT
ATGCAAGATATTGAATTTACAGTTGAAAATAAAAAGCTCTATTTATTGCAAACTAGAAATGGAAAGAGAACAGCAGCTGC
TGCTGTAAAAATTGCAGTGGATATGGTTGAAGAGGGAGTAATATCGAAAGAAACTGCATTACAAAGAGTAGAACCAGAAC
AAATTGCCCATTTATTGCACCCACAAATTGATCCCGATCAGGACTTTGAAATGTTGACCAGTGGTTTACCTGCTTCGCCC
GGTGCTGCTACTGGCAATGTGGTCTTTGATGCCAATGAAGCTGAGGAGTTAAAAAACATTGGTAAAAATGTCATCCTAGC
TAGACCAGAAACTACCCCTGACGATATACATGGGATAGTTGCAGCAGAAGGAATACTAACAAGTCGTGGTGGCATGACTA
GTCATGCAGCTGTTGTGGCTAGAGGTATGGGTATTCCAGCCGTTTGTGGTTGTGATGAAATAAGTATTGATCTTGAGAAT
GAGAGAATGATCATTAACGGCCAAAACATAAATAAGGGTGAGTTAATTTCCATTGATGGTGGTAGTGGTAAAGTAATCTC
TGGAGCTGTAAAATTAACGGACCCTGGACTAAATGAAGAAAGTCAAAAATTACTTGATTGGGCAGAAAATATCAAACAAT
TAGGTGTGAGAGCTAATGCAGACACACCATCTGATGCCGCAAAAGCAAGAGAGTTTGGGGCCAAAGGGATTGGTCTTTGT
AGAACTGAACACATGTTTATGGGTTCAGAGCGCTTACCTAAGGTTCAGGAAATGATTATTTCAGAGTCTGAAAGCCAAAG
GAAAGAAGCTTTAGATAAATTGGTCCCATATCAAGAACAAGATTTTTATGAAATCTTAAAAATTATGGATGGATATCCTA
CCACTGTAAGATTATTGGATCCGCCACTCCATGAATTTTTGCCCGACAGAGAAAAACTTGAACAAGAATTGAATAACTTA
TCGAAAGATGAGATTGATGAAGAGGCCTTAGAAGAGAAAAAACAACTTCTTGATAAAGTTAAAGCCCTAGAAGAATCTAA
TCCTATGCTAGGATTTAGGGGCTGTCGTTTAGGATTAATATACCCAGAAATTTATAATATGCAGGTGGAAGCCCTGGTCA
AAGCAACTAAAAAGCTATTGGCTGAAGGATACTCACCGCAACCAGAGATTATGGTACCTTTAGTAGGACATGAAACAGAA
ATGGAGTTATTAAAAGATAGCATTCATGAAATAATAGACTTACATGTAGAAGCACCAGAAGAAAAAGAAATCTTCCATGT
TGGGACCATGATCGAATTACCCAGAGCTTGTATGACAGCTGATGAAATTGCCAATCATGTTCAATTCTTTTCTTTTGGTA
CTAATGATCTTACACAAACAACTTTAGGATATAGTAGAGATGATGCAGAAGGAAAGTTCTTAGCTCATTATATGCAAAAT
GACATTATTCCTGAAAACCCTTTTATGATTTTAGACCAGGCAGGAGTGGGTGAACTAGTTAAAATAGCAGTTGAAAAATC
AAAACAGGTCAATCCAGATTTAACTAGAGGTATTTGTGGTGAACACGGAGGCGATCCTGATTCTATTAAATTCTTCCATA
AGATTGGACTACACTATGTTAGTTGTTCTCCTTTTAGGGTTCCTGTAGCAAGGTTGGCTGCAGCACAATCTGTTTTAAAT
TCAGAATAA

Upstream 100 bases:

>100_bases
ATAATGAAAAAAATTGGTTGTCCTACCATTGACGTTAGTAATCGGGCTGTGGAGGAAACGGCCCAAAAAATACTAGACAT
GATTAGAGGGGGTAAAGATA

Downstream 100 bases:

>100_bases
GTATAATTTCTTTTTTCTTTGTTATGTATTTATTTTGGTTTACACATTACAAGTAATGTAAAAATGCTCCCCTTTAGGTA
CAGATTTGTTTTTAACTGCA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 882; Mature: 881

Protein sequence:

>882_residues
MTSMDKLVYKFSEGSRDMKPFLGGKGANLAEMTKIGLPVPPGFTVSTDACNEYFKNNGILTEQLREQIFTALSEIENATN
KKFGDPSNPLLVSVRSGAVISMPGMMDTVLNLGLNDETVQGLARQTSNDRFAYDSYRRFIQMFSDVVMGIGNYMFENEMS
RLKEEYQVTEDTQLNSQALQDLITRYKQVYNRETGEEFPQDPKDQLIMAVKAVFNSWNNQRAKVYREIHNISHELGTAVN
VQTMVFGNMGNDSGTGVAFTRNPSTGEKNIYGEFLLNAQGEDVVAGIRTPKAIKDLEDEFPEVYNQFLEVSQLLENHYCD
MQDIEFTVENKKLYLLQTRNGKRTAAAAVKIAVDMVEEGVISKETALQRVEPEQIAHLLHPQIDPDQDFEMLTSGLPASP
GAATGNVVFDANEAEELKNIGKNVILARPETTPDDIHGIVAAEGILTSRGGMTSHAAVVARGMGIPAVCGCDEISIDLEN
ERMIINGQNINKGELISIDGGSGKVISGAVKLTDPGLNEESQKLLDWAENIKQLGVRANADTPSDAAKAREFGAKGIGLC
RTEHMFMGSERLPKVQEMIISESESQRKEALDKLVPYQEQDFYEILKIMDGYPTTVRLLDPPLHEFLPDREKLEQELNNL
SKDEIDEEALEEKKQLLDKVKALEESNPMLGFRGCRLGLIYPEIYNMQVEALVKATKKLLAEGYSPQPEIMVPLVGHETE
MELLKDSIHEIIDLHVEAPEEKEIFHVGTMIELPRACMTADEIANHVQFFSFGTNDLTQTTLGYSRDDAEGKFLAHYMQN
DIIPENPFMILDQAGVGELVKIAVEKSKQVNPDLTRGICGEHGGDPDSIKFFHKIGLHYVSCSPFRVPVARLAAAQSVLN
SE

Sequences:

>Translated_882_residues
MTSMDKLVYKFSEGSRDMKPFLGGKGANLAEMTKIGLPVPPGFTVSTDACNEYFKNNGILTEQLREQIFTALSEIENATN
KKFGDPSNPLLVSVRSGAVISMPGMMDTVLNLGLNDETVQGLARQTSNDRFAYDSYRRFIQMFSDVVMGIGNYMFENEMS
RLKEEYQVTEDTQLNSQALQDLITRYKQVYNRETGEEFPQDPKDQLIMAVKAVFNSWNNQRAKVYREIHNISHELGTAVN
VQTMVFGNMGNDSGTGVAFTRNPSTGEKNIYGEFLLNAQGEDVVAGIRTPKAIKDLEDEFPEVYNQFLEVSQLLENHYCD
MQDIEFTVENKKLYLLQTRNGKRTAAAAVKIAVDMVEEGVISKETALQRVEPEQIAHLLHPQIDPDQDFEMLTSGLPASP
GAATGNVVFDANEAEELKNIGKNVILARPETTPDDIHGIVAAEGILTSRGGMTSHAAVVARGMGIPAVCGCDEISIDLEN
ERMIINGQNINKGELISIDGGSGKVISGAVKLTDPGLNEESQKLLDWAENIKQLGVRANADTPSDAAKAREFGAKGIGLC
RTEHMFMGSERLPKVQEMIISESESQRKEALDKLVPYQEQDFYEILKIMDGYPTTVRLLDPPLHEFLPDREKLEQELNNL
SKDEIDEEALEEKKQLLDKVKALEESNPMLGFRGCRLGLIYPEIYNMQVEALVKATKKLLAEGYSPQPEIMVPLVGHETE
MELLKDSIHEIIDLHVEAPEEKEIFHVGTMIELPRACMTADEIANHVQFFSFGTNDLTQTTLGYSRDDAEGKFLAHYMQN
DIIPENPFMILDQAGVGELVKIAVEKSKQVNPDLTRGICGEHGGDPDSIKFFHKIGLHYVSCSPFRVPVARLAAAQSVLN
SE
>Mature_881_residues
TSMDKLVYKFSEGSRDMKPFLGGKGANLAEMTKIGLPVPPGFTVSTDACNEYFKNNGILTEQLREQIFTALSEIENATNK
KFGDPSNPLLVSVRSGAVISMPGMMDTVLNLGLNDETVQGLARQTSNDRFAYDSYRRFIQMFSDVVMGIGNYMFENEMSR
LKEEYQVTEDTQLNSQALQDLITRYKQVYNRETGEEFPQDPKDQLIMAVKAVFNSWNNQRAKVYREIHNISHELGTAVNV
QTMVFGNMGNDSGTGVAFTRNPSTGEKNIYGEFLLNAQGEDVVAGIRTPKAIKDLEDEFPEVYNQFLEVSQLLENHYCDM
QDIEFTVENKKLYLLQTRNGKRTAAAAVKIAVDMVEEGVISKETALQRVEPEQIAHLLHPQIDPDQDFEMLTSGLPASPG
AATGNVVFDANEAEELKNIGKNVILARPETTPDDIHGIVAAEGILTSRGGMTSHAAVVARGMGIPAVCGCDEISIDLENE
RMIINGQNINKGELISIDGGSGKVISGAVKLTDPGLNEESQKLLDWAENIKQLGVRANADTPSDAAKAREFGAKGIGLCR
TEHMFMGSERLPKVQEMIISESESQRKEALDKLVPYQEQDFYEILKIMDGYPTTVRLLDPPLHEFLPDREKLEQELNNLS
KDEIDEEALEEKKQLLDKVKALEESNPMLGFRGCRLGLIYPEIYNMQVEALVKATKKLLAEGYSPQPEIMVPLVGHETEM
ELLKDSIHEIIDLHVEAPEEKEIFHVGTMIELPRACMTADEIANHVQFFSFGTNDLTQTTLGYSRDDAEGKFLAHYMQND
IIPENPFMILDQAGVGELVKIAVEKSKQVNPDLTRGICGEHGGDPDSIKFFHKIGLHYVSCSPFRVPVARLAAAQSVLNS
E

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=470, Percent_Identity=26.5957446808511, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1787994, Length=905, Percent_Identity=22.2099447513812, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI1789193, Length=462, Percent_Identity=24.6753246753247, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1788726, Length=448, Percent_Identity=22.9910714285714, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI48994992, Length=457, Percent_Identity=24.507658643326, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 98023; Mature: 97892

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSMDKLVYKFSEGSRDMKPFLGGKGANLAEMTKIGLPVPPGFTVSTDACNEYFKNNGIL
CCCHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHCCCCCCCCCEECHHHHHHHHHCCCEE
TEQLREQIFTALSEIENATNKKFGDPSNPLLVSVRSGAVISMPGMMDTVLNLGLNDETVQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEECCCHHHHHHHCCCCHHHHH
GLARQTSNDRFAYDSYRRFIQMFSDVVMGIGNYMFENEMSRLKEEYQVTEDTQLNSQALQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DLITRYKQVYNRETGEEFPQDPKDQLIMAVKAVFNSWNNQRAKVYREIHNISHELGTAVN
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEE
VQTMVFGNMGNDSGTGVAFTRNPSTGEKNIYGEFLLNAQGEDVVAGIRTPKAIKDLEDEF
EEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCEEECCCCCHHHHHHHHHH
PEVYNQFLEVSQLLENHYCDMQDIEFTVENKKLYLLQTRNGKRTAAAAVKIAVDMVEEGV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCC
ISKETALQRVEPEQIAHLLHPQIDPDQDFEMLTSGLPASPGAATGNVVFDANEAEELKNI
CHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHC
GKNVILARPETTPDDIHGIVAAEGILTSRGGMTSHAAVVARGMGIPAVCGCDEISIDLEN
CCCEEEECCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHCCCCCCEECCCCEEEEECC
ERMIINGQNINKGELISIDGGSGKVISGAVKLTDPGLNEESQKLLDWAENIKQLGVRANA
CEEEEECCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
DTPSDAAKAREFGAKGIGLCRTEHMFMGSERLPKVQEMIISESESQRKEALDKLVPYQEQ
CCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCC
DFYEILKIMDGYPTTVRLLDPPLHEFLPDREKLEQELNNLSKDEIDEEALEEKKQLLDKV
CHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KALEESNPMLGFRGCRLGLIYPEIYNMQVEALVKATKKLLAEGYSPQPEIMVPLVGHETE
HHHHCCCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHH
MELLKDSIHEIIDLHVEAPEEKEIFHVGTMIELPRACMTADEIANHVQFFSFGTNDLTQT
HHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHH
TLGYSRDDAEGKFLAHYMQNDIIPENPFMILDQAGVGELVKIAVEKSKQVNPDLTRGICG
HCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHCC
EHGGDPDSIKFFHKIGLHYVSCSPFRVPVARLAAAQSVLNSE
CCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSMDKLVYKFSEGSRDMKPFLGGKGANLAEMTKIGLPVPPGFTVSTDACNEYFKNNGIL
CCHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHCCCCCCCCCEECHHHHHHHHHCCCEE
TEQLREQIFTALSEIENATNKKFGDPSNPLLVSVRSGAVISMPGMMDTVLNLGLNDETVQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEECCCHHHHHHHCCCCHHHHH
GLARQTSNDRFAYDSYRRFIQMFSDVVMGIGNYMFENEMSRLKEEYQVTEDTQLNSQALQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DLITRYKQVYNRETGEEFPQDPKDQLIMAVKAVFNSWNNQRAKVYREIHNISHELGTAVN
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEE
VQTMVFGNMGNDSGTGVAFTRNPSTGEKNIYGEFLLNAQGEDVVAGIRTPKAIKDLEDEF
EEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCEEECCCCCHHHHHHHHHH
PEVYNQFLEVSQLLENHYCDMQDIEFTVENKKLYLLQTRNGKRTAAAAVKIAVDMVEEGV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCC
ISKETALQRVEPEQIAHLLHPQIDPDQDFEMLTSGLPASPGAATGNVVFDANEAEELKNI
CHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHC
GKNVILARPETTPDDIHGIVAAEGILTSRGGMTSHAAVVARGMGIPAVCGCDEISIDLEN
CCCEEEECCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHCCCCCCEECCCCEEEEECC
ERMIINGQNINKGELISIDGGSGKVISGAVKLTDPGLNEESQKLLDWAENIKQLGVRANA
CEEEEECCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
DTPSDAAKAREFGAKGIGLCRTEHMFMGSERLPKVQEMIISESESQRKEALDKLVPYQEQ
CCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCC
DFYEILKIMDGYPTTVRLLDPPLHEFLPDREKLEQELNNLSKDEIDEEALEEKKQLLDKV
CHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KALEESNPMLGFRGCRLGLIYPEIYNMQVEALVKATKKLLAEGYSPQPEIMVPLVGHETE
HHHHCCCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHH
MELLKDSIHEIIDLHVEAPEEKEIFHVGTMIELPRACMTADEIANHVQFFSFGTNDLTQT
HHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHH
TLGYSRDDAEGKFLAHYMQNDIIPENPFMILDQAGVGELVKIAVEKSKQVNPDLTRGICG
HCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHCC
EHGGDPDSIKFFHKIGLHYVSCSPFRVPVARLAAAQSVLNSE
CCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]