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The map label for this gene is asnB [H]

Identifier: 188585588

GI number: 188585588

Start: 1019217

End: 1021109

Strand: Direct

Name: asnB [H]

Synonym: Nther_0961

Alternate gene names: 188585588

Gene position: 1019217-1021109 (Clockwise)

Preceding gene: 188585584

Following gene: 188585589

Centisome position: 32.2

GC content: 31.85

Gene sequence:

>1893_bases
ATGTGTGGGTTTGTTTGTTATATACCTAAAAAAGAAAAAGGTAATTTGTATAGTAAAAACATGAAAATAAAAATTCAGAA
TAAGGTGAAAGATGCCGCAGAAGTTATAAGTCATAGAGGCCCAGATGATAGTGGTTATTATAGCGATAAAAATATTTCTT
TAGGGTTTAAACGACTAAGTATTATTGACTTGGAAGGCGGGAAGCAACCACTATCTTATTTTAATAACCGTTACCAAATA
GTTTTTAATGGGGAAATTTACAATTACCTAGAATTAAGAAATGAATTAATCAATACTGGAGTTAATTTAAACACAGAATC
AGATACTGAAATTATTGTTGCTTTATATCATCAGCATGGTTCTGAAATAGCTCATAAATTAAGGGGTATGTTTTCTTTTA
TTATTTGGGATTCAAAGCTAAAGAAACTGTTTGGTGCTAGAGACTATTTTGGTATAAAGCCGTTGTATTATTCAGACCAT
GATGAGTTTATAGTATTTTGTTCAGAGAAAAAAGCGTTGTTACAATTTATAGAAACTGACCTGGATTACGAGACTTTACA
ACATTATCTTACTTATCAGTATCCCCCGGAACCTAATACAATGGATAAAAACATTAAAAAAATACCGCCAGGTACTTGCT
TTTACTATGATACCAAAAGTCAATCTGAGTGTTTATCTTTAAATAAATATTTCAGTCTAAAACTCAATCCTAATAAAAAC
TACATCAATCAAGAAGAAAAGTTGGTTAGTCAAGTTAAAGTTGCTTTAGAAAAATCTGTTAAATTGCATATGAGAAGTGA
TGTTCCAGTAGGTGCCTTTTTATCAAGTGGTATAGATTCTACTGGTATAGTAGCTTTAGCTAAAAAGATTCATCCAGATA
TCAAAACTTTTACGAGTGGATTTTCAAGAGAAGGTTATAATGAAGCTGATATAGCTAAAGATACGGCAGAAAAAATAGGT
GTGGATCATTACACTAAAACTGTAACTGCGGAAGATATTATGAGAGACTTGCCAACAATAATTTGGCATATGGATGACCC
GGTTGCTGACCCCGCAGCCATTCCTTTGTATTACGTTAGTCAAGAAGCTTCAAAACAGGTTAAAGTTGTCTTATCAGGTG
AAGGTGCTGATGAATTGTTTGGAGGATACAATATTTATAGAGAACCAGGGGATTTGAAAAAGTTCACTTCTTTACCAATT
ACCTTAAAAAAATCATTATTTTATTTAAGTCGGATTCTACCTGATGGTGTAAAAGGAAAAGAGTTTATTAGTAGAGGTAT
TCTTCCTATTGAAAAAAGGTATTATGGTAATGCTAGGATTTTTAAAGAGTTAGAAAAAGCTAAGTTTTATCAAAAGTTTG
ATTCTGGAAAAAAATTTTATGATATTACTCAATCCTATTATAAAGAAGCTAAAAACATGGAAAGCATAGAAAAAATGCAG
TATATTGACTTGAACACCTGGCTTTCCGGAGACATACTTATTAAGGCAGATAGAATGACTATGGCACATTCTTTAGAATT
GCGGGTTCCCTTTTTAGATAAAAGTGTTTTTAATATTGCGAAGCAAATACCATCTGAATTTAAGGTAACTCCAGAAACTA
CTAAATATATTTTGAGAAAAGCTCTGGAAACCGAGATACCCGATTCTATCATAAACAGAAAAAAATTAGGATTCCCGGTT
CCCATAAGATTGTGGTTAAAAGAAGAAATGTTTTCTTGGGCAAGAACCATTATCAAAAATAGTGACACAGATTATTTATT
CAACAAGCAAGCGGTATTAAAGCTACTAGAAGATCACCGAAAAAACAAGGGAGACTATAGCCGCAAAATTTGGACTGTAC
TAACTTTTATGATTTGGCATAAAAATATGGTGGTAGAAAGAAAGGCTTATTAA

Upstream 100 bases:

>100_bases
GTTTCGTGCATTTTATCATGAGTTAACAAAGAATATCATACACGAGTACACTATTAATGTGATTTATCCTGGCAAAACAA
GTAACAGAAAGGATGGTAAA

Downstream 100 bases:

>100_bases
TTGGTTAAGCAGATTTAATAAATTACTTTATTAATAATCACATCTTATAGTAAATAATTATAATATATAGCAACTAAAAT
TTATAAACTTTGAGTGCAAA

Product: asparagine synthase (glutamine-hydrolysing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MCGFVCYIPKKEKGNLYSKNMKIKIQNKVKDAAEVISHRGPDDSGYYSDKNISLGFKRLSIIDLEGGKQPLSYFNNRYQI
VFNGEIYNYLELRNELINTGVNLNTESDTEIIVALYHQHGSEIAHKLRGMFSFIIWDSKLKKLFGARDYFGIKPLYYSDH
DEFIVFCSEKKALLQFIETDLDYETLQHYLTYQYPPEPNTMDKNIKKIPPGTCFYYDTKSQSECLSLNKYFSLKLNPNKN
YINQEEKLVSQVKVALEKSVKLHMRSDVPVGAFLSSGIDSTGIVALAKKIHPDIKTFTSGFSREGYNEADIAKDTAEKIG
VDHYTKTVTAEDIMRDLPTIIWHMDDPVADPAAIPLYYVSQEASKQVKVVLSGEGADELFGGYNIYREPGDLKKFTSLPI
TLKKSLFYLSRILPDGVKGKEFISRGILPIEKRYYGNARIFKELEKAKFYQKFDSGKKFYDITQSYYKEAKNMESIEKMQ
YIDLNTWLSGDILIKADRMTMAHSLELRVPFLDKSVFNIAKQIPSEFKVTPETTKYILRKALETEIPDSIINRKKLGFPV
PIRLWLKEEMFSWARTIIKNSDTDYLFNKQAVLKLLEDHRKNKGDYSRKIWTVLTFMIWHKNMVVERKAY

Sequences:

>Translated_630_residues
MCGFVCYIPKKEKGNLYSKNMKIKIQNKVKDAAEVISHRGPDDSGYYSDKNISLGFKRLSIIDLEGGKQPLSYFNNRYQI
VFNGEIYNYLELRNELINTGVNLNTESDTEIIVALYHQHGSEIAHKLRGMFSFIIWDSKLKKLFGARDYFGIKPLYYSDH
DEFIVFCSEKKALLQFIETDLDYETLQHYLTYQYPPEPNTMDKNIKKIPPGTCFYYDTKSQSECLSLNKYFSLKLNPNKN
YINQEEKLVSQVKVALEKSVKLHMRSDVPVGAFLSSGIDSTGIVALAKKIHPDIKTFTSGFSREGYNEADIAKDTAEKIG
VDHYTKTVTAEDIMRDLPTIIWHMDDPVADPAAIPLYYVSQEASKQVKVVLSGEGADELFGGYNIYREPGDLKKFTSLPI
TLKKSLFYLSRILPDGVKGKEFISRGILPIEKRYYGNARIFKELEKAKFYQKFDSGKKFYDITQSYYKEAKNMESIEKMQ
YIDLNTWLSGDILIKADRMTMAHSLELRVPFLDKSVFNIAKQIPSEFKVTPETTKYILRKALETEIPDSIINRKKLGFPV
PIRLWLKEEMFSWARTIIKNSDTDYLFNKQAVLKLLEDHRKNKGDYSRKIWTVLTFMIWHKNMVVERKAY
>Mature_630_residues
MCGFVCYIPKKEKGNLYSKNMKIKIQNKVKDAAEVISHRGPDDSGYYSDKNISLGFKRLSIIDLEGGKQPLSYFNNRYQI
VFNGEIYNYLELRNELINTGVNLNTESDTEIIVALYHQHGSEIAHKLRGMFSFIIWDSKLKKLFGARDYFGIKPLYYSDH
DEFIVFCSEKKALLQFIETDLDYETLQHYLTYQYPPEPNTMDKNIKKIPPGTCFYYDTKSQSECLSLNKYFSLKLNPNKN
YINQEEKLVSQVKVALEKSVKLHMRSDVPVGAFLSSGIDSTGIVALAKKIHPDIKTFTSGFSREGYNEADIAKDTAEKIG
VDHYTKTVTAEDIMRDLPTIIWHMDDPVADPAAIPLYYVSQEASKQVKVVLSGEGADELFGGYNIYREPGDLKKFTSLPI
TLKKSLFYLSRILPDGVKGKEFISRGILPIEKRYYGNARIFKELEKAKFYQKFDSGKKFYDITQSYYKEAKNMESIEKMQ
YIDLNTWLSGDILIKADRMTMAHSLELRVPFLDKSVFNIAKQIPSEFKVTPETTKYILRKALETEIPDSIINRKKLGFPV
PIRLWLKEEMFSWARTIIKNSDTDYLFNKQAVLKLLEDHRKNKGDYSRKIWTVLTFMIWHKNMVVERKAY

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=382, Percent_Identity=28.7958115183246, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI168229252, Length=382, Percent_Identity=28.7958115183246, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI168229250, Length=382, Percent_Identity=28.7958115183246, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI296010848, Length=378, Percent_Identity=28.8359788359788, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI296010852, Length=303, Percent_Identity=28.0528052805281, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI296010850, Length=303, Percent_Identity=28.0528052805281, Blast_Score=94, Evalue=5e-19,
Organism=Escherichia coli, GI1786889, Length=396, Percent_Identity=30.8080808080808, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71993933, Length=360, Percent_Identity=33.0555555555556, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25147557, Length=360, Percent_Identity=33.0555555555556, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17560178, Length=382, Percent_Identity=27.2251308900524, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25147560, Length=298, Percent_Identity=30.8724832214765, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321563, Length=442, Percent_Identity=27.8280542986425, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6325403, Length=408, Percent_Identity=28.4313725490196, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45553209, Length=358, Percent_Identity=30.4469273743017, Blast_Score=149, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72966; Mature: 72966

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFVCYIPKKEKGNLYSKNMKIKIQNKVKDAAEVISHRGPDDSGYYSDKNISLGFKRLS
CCCEEEECCCCCCCCEEECCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEECEEEEE
IIDLEGGKQPLSYFNNRYQIVFNGEIYNYLELRNELINTGVNLNTESDTEIIVALYHQHG
EEEECCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHCC
SEIAHKLRGMFSFIIWDSKLKKLFGARDYFGIKPLYYSDHDEFIVFCSEKKALLQFIETD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHHHC
LDYETLQHYLTYQYPPEPNTMDKNIKKIPPGTCFYYDTKSQSECLSLNKYFSLKLNPNKN
CCHHHHHHHEEEECCCCCCCCCCCHHCCCCCEEEEECCCCCHHHHCCCEEEEEEECCCCC
YINQEEKLVSQVKVALEKSVKLHMRSDVPVGAFLSSGIDSTGIVALAKKIHPDIKTFTSG
CCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCHHHHHCC
FSREGYNEADIAKDTAEKIGVDHYTKTVTAEDIMRDLPTIIWHMDDPVADPAAIPLYYVS
CCCCCCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHEEEECCCCCCCCCCCEEEEEC
QEASKQVKVVLSGEGADELFGGYNIYREPGDLKKFTSLPITLKKSLFYLSRILPDGVKGK
CCCCCEEEEEEECCCHHHHHCCCEEECCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCH
EFISRGILPIEKRYYGNARIFKELEKAKFYQKFDSGKKFYDITQSYYKEAKNMESIEKMQ
HHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHH
YIDLNTWLSGDILIKADRMTMAHSLELRVPFLDKSVFNIAKQIPSEFKVTPETTKYILRK
EEECCCCCCCCEEEEECCEEEEEEEEEECCHHHHHHHHHHHHCCHHEECCCHHHHHHHHH
ALETEIPDSIINRKKLGFPVPIRLWLKEEMFSWARTIIKNSDTDYLFNKQAVLKLLEDHR
HHHHCCCHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHC
KNKGDYSRKIWTVLTFMIWHKNMVVERKAY
CCCCHHHHHHHHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure
MCGFVCYIPKKEKGNLYSKNMKIKIQNKVKDAAEVISHRGPDDSGYYSDKNISLGFKRLS
CCCEEEECCCCCCCCEEECCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEECEEEEE
IIDLEGGKQPLSYFNNRYQIVFNGEIYNYLELRNELINTGVNLNTESDTEIIVALYHQHG
EEEECCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHCC
SEIAHKLRGMFSFIIWDSKLKKLFGARDYFGIKPLYYSDHDEFIVFCSEKKALLQFIETD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHHHC
LDYETLQHYLTYQYPPEPNTMDKNIKKIPPGTCFYYDTKSQSECLSLNKYFSLKLNPNKN
CCHHHHHHHEEEECCCCCCCCCCCHHCCCCCEEEEECCCCCHHHHCCCEEEEEEECCCCC
YINQEEKLVSQVKVALEKSVKLHMRSDVPVGAFLSSGIDSTGIVALAKKIHPDIKTFTSG
CCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHCCCCCCHHHHHHHHHCCCHHHHHCC
FSREGYNEADIAKDTAEKIGVDHYTKTVTAEDIMRDLPTIIWHMDDPVADPAAIPLYYVS
CCCCCCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHEEEECCCCCCCCCCCEEEEEC
QEASKQVKVVLSGEGADELFGGYNIYREPGDLKKFTSLPITLKKSLFYLSRILPDGVKGK
CCCCCEEEEEEECCCHHHHHCCCEEECCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCH
EFISRGILPIEKRYYGNARIFKELEKAKFYQKFDSGKKFYDITQSYYKEAKNMESIEKMQ
HHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHH
YIDLNTWLSGDILIKADRMTMAHSLELRVPFLDKSVFNIAKQIPSEFKVTPETTKYILRK
EEECCCCCCCCEEEEECCEEEEEEEEEECCHHHHHHHHHHHHCCHHEECCCHHHHHHHHH
ALETEIPDSIINRKKLGFPVPIRLWLKEEMFSWARTIIKNSDTDYLFNKQAVLKLLEDHR
HHHHCCCHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHC
KNKGDYSRKIWTVLTFMIWHKNMVVERKAY
CCCCHHHHHHHHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]