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The map label for this gene is glnA [H]

Identifier: 188585579

GI number: 188585579

Start: 1008449

End: 1009786

Strand: Direct

Name: glnA [H]

Synonym: Nther_0952

Alternate gene names: 188585579

Gene position: 1008449-1009786 (Clockwise)

Preceding gene: 188585578

Following gene: 188585582

Centisome position: 31.86

GC content: 35.05

Gene sequence:

>1338_bases
ATGAGTGACCTTACAAGAGAAGAATTGAAAGAAAAAATTTCTGATTTGGACATCGAATTTATTCGTCTACAGTTTACTGA
TGTTTTTGGTATTATGAAAAGTATGTCTGTCACTGTTGACGAGTTAGATGCGGCTATGGATGGTGAACTGATGTTTGATG
GGTCTTCAATTGATGGTTTTGCTAGGATTGAAGAATCTGATCAGGTATTAATCCCGGATACTTCAACTTTTAATGTTATG
CCCTGGAGACCATCGGAAAAAGGTGTTGCTAGCATGATTTGTGATGTTTACAACACTGATGGTACACCATTTGAAGGATG
TCCAAGAAATATCTTAAAAAAAGCCGTACAAGAAGCAGAAGAGATGGGATATGAATTTAATGTAGGACCTGAAGGAGAAT
TTTTTCTATTTCACACTGATGAAAAAGGACGCCCGGTATTTGATGTACATGATACAGCAGGATACTTTGATCTAGCTCCC
TATGATATGGGGGAAGATGCCAGAAGAAGTATTATATTGACGATGAAAAAAATGGGCTTTAGGATTGAAGCCTCTCACCA
CGAAGTAGCGAAAGGCCAACATGAAATAGATTTCAAGTATGATGAAGCAGTAACTACAGCTGATAATTGGATGACTTTTA
AAGATATTGTCAAAAATATTGCAAAAAATCACGGATTGTATGCAACTTTCTTGCCCAAGCCATTTTCTAACGACAATGGC
AATGCAATGCACTGTAACCAATCTTTATTTAAAAATGGAGAAAATGCTTTTTATGACTGTAATGATCAAGAAAATGGGCA
ATTAAGCGATATTGCTTATTACTATATTGGTGGATTGTTAAAACATGCTAAAGGTATGACTTTAGTTGCCAACCCAATCG
TTAACAGTTATAAACGCTTATTACCAGGATATGAAGCACCTACTAACATTGCTTGGTCAACTAAAAATCGCAGTACACTT
GTAAGAGTGCCTGCTTCTAGGGGATGTAGTACCAGAATTGAACTTAGAAATCCAGATCCAACTGCTAATCCATATCTAAT
TTTTGCTGTTATGCTGAAGGCAGGTTTAGAAGGGATTAAAAATAAGATTACTCCACCACCAGATATAGAAGAGAATATAT
TTGAACTTTCCCTTGAAGAACAACAAAAGCTAAATGTAGATTTATTACCCAGAGATATGTACGAAGCCATTAATGAAATG
AAAAAAGATTCTTTAGTAAGAGAAGCTCTTGGTGAACATGTTTACAATTTATATTTAAAGGCTAAATTTGACGAATGGGT
TTCATATAACAAACAAGTGCATGATTGGGAAATTGATAATTATCTATCCAAGTATTAA

Upstream 100 bases:

>100_bases
TGAAACCTTATAATATTTTTACCTTAACTTGACATTATTTTAGCTTATGATAAAATTTTACATTAGATTTACAAATTTTG
TAGGAGGCGATATTTAATTC

Downstream 100 bases:

>100_bases
AAATAAATAAGCTAGCCAGATTTTTTCATCTACCTTAGGGGTTGTCCAAGCGACAACCCCTATCTTAATTTCGCATGTCC
CAGTATTAATTATGAGCTGG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSDLTREELKEKISDLDIEFIRLQFTDVFGIMKSMSVTVDELDAAMDGELMFDGSSIDGFARIEESDQVLIPDTSTFNVM
PWRPSEKGVASMICDVYNTDGTPFEGCPRNILKKAVQEAEEMGYEFNVGPEGEFFLFHTDEKGRPVFDVHDTAGYFDLAP
YDMGEDARRSIILTMKKMGFRIEASHHEVAKGQHEIDFKYDEAVTTADNWMTFKDIVKNIAKNHGLYATFLPKPFSNDNG
NAMHCNQSLFKNGENAFYDCNDQENGQLSDIAYYYIGGLLKHAKGMTLVANPIVNSYKRLLPGYEAPTNIAWSTKNRSTL
VRVPASRGCSTRIELRNPDPTANPYLIFAVMLKAGLEGIKNKITPPPDIEENIFELSLEEQQKLNVDLLPRDMYEAINEM
KKDSLVREALGEHVYNLYLKAKFDEWVSYNKQVHDWEIDNYLSKY

Sequences:

>Translated_445_residues
MSDLTREELKEKISDLDIEFIRLQFTDVFGIMKSMSVTVDELDAAMDGELMFDGSSIDGFARIEESDQVLIPDTSTFNVM
PWRPSEKGVASMICDVYNTDGTPFEGCPRNILKKAVQEAEEMGYEFNVGPEGEFFLFHTDEKGRPVFDVHDTAGYFDLAP
YDMGEDARRSIILTMKKMGFRIEASHHEVAKGQHEIDFKYDEAVTTADNWMTFKDIVKNIAKNHGLYATFLPKPFSNDNG
NAMHCNQSLFKNGENAFYDCNDQENGQLSDIAYYYIGGLLKHAKGMTLVANPIVNSYKRLLPGYEAPTNIAWSTKNRSTL
VRVPASRGCSTRIELRNPDPTANPYLIFAVMLKAGLEGIKNKITPPPDIEENIFELSLEEQQKLNVDLLPRDMYEAINEM
KKDSLVREALGEHVYNLYLKAKFDEWVSYNKQVHDWEIDNYLSKY
>Mature_444_residues
SDLTREELKEKISDLDIEFIRLQFTDVFGIMKSMSVTVDELDAAMDGELMFDGSSIDGFARIEESDQVLIPDTSTFNVMP
WRPSEKGVASMICDVYNTDGTPFEGCPRNILKKAVQEAEEMGYEFNVGPEGEFFLFHTDEKGRPVFDVHDTAGYFDLAPY
DMGEDARRSIILTMKKMGFRIEASHHEVAKGQHEIDFKYDEAVTTADNWMTFKDIVKNIAKNHGLYATFLPKPFSNDNGN
AMHCNQSLFKNGENAFYDCNDQENGQLSDIAYYYIGGLLKHAKGMTLVANPIVNSYKRLLPGYEAPTNIAWSTKNRSTLV
RVPASRGCSTRIELRNPDPTANPYLIFAVMLKAGLEGIKNKITPPPDIEENIFELSLEEQQKLNVDLLPRDMYEAINEMK
KDSLVREALGEHVYNLYLKAKFDEWVSYNKQVHDWEIDNYLSKY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=435, Percent_Identity=22.2988505747126, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1790301, Length=476, Percent_Identity=36.7647058823529, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI87081870, Length=395, Percent_Identity=26.0759493670886, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50713; Mature: 50582

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLTREELKEKISDLDIEFIRLQFTDVFGIMKSMSVTVDELDAAMDGELMFDGSSIDGF
CCCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCCE
ARIEESDQVLIPDTSTFNVMPWRPSEKGVASMICDVYNTDGTPFEGCPRNILKKAVQEAE
EEECCCCCEECCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EMGYEFNVGPEGEFFLFHTDEKGRPVFDVHDTAGYFDLAPYDMGEDARRSIILTMKKMGF
HCCCEECCCCCCCEEEEEECCCCCEEEEECCCCCEEECCCCCCCCHHHHHEEEEEHHCCE
RIEASHHEVAKGQHEIDFKYDEAVTTADNWMTFKDIVKNIAKNHGLYATFLPKPFSNDNG
EEECCCHHHHCCCCCCCEEECCCEECCCCCEEHHHHHHHHHHCCCEEEEECCCCCCCCCC
NAMHCNQSLFKNGENAFYDCNDQENGQLSDIAYYYIGGLLKHAKGMTLVANPIVNSYKRL
CEEEECHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
LPGYEAPTNIAWSTKNRSTLVRVPASRGCSTRIELRNPDPTANPYLIFAVMLKAGLEGIK
CCCCCCCCCCEEECCCCCEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
NKITPPPDIEENIFELSLEEQQKLNVDLLPRDMYEAINEMKKDSLVREALGEHVYNLYLK
HCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
AKFDEWVSYNKQVHDWEIDNYLSKY
EECHHHHHCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
SDLTREELKEKISDLDIEFIRLQFTDVFGIMKSMSVTVDELDAAMDGELMFDGSSIDGF
CCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCCE
ARIEESDQVLIPDTSTFNVMPWRPSEKGVASMICDVYNTDGTPFEGCPRNILKKAVQEAE
EEECCCCCEECCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EMGYEFNVGPEGEFFLFHTDEKGRPVFDVHDTAGYFDLAPYDMGEDARRSIILTMKKMGF
HCCCEECCCCCCCEEEEEECCCCCEEEEECCCCCEEECCCCCCCCHHHHHEEEEEHHCCE
RIEASHHEVAKGQHEIDFKYDEAVTTADNWMTFKDIVKNIAKNHGLYATFLPKPFSNDNG
EEECCCHHHHCCCCCCCEEECCCEECCCCCEEHHHHHHHHHHCCCEEEEECCCCCCCCCC
NAMHCNQSLFKNGENAFYDCNDQENGQLSDIAYYYIGGLLKHAKGMTLVANPIVNSYKRL
CEEEECHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
LPGYEAPTNIAWSTKNRSTLVRVPASRGCSTRIELRNPDPTANPYLIFAVMLKAGLEGIK
CCCCCCCCCCEEECCCCCEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
NKITPPPDIEENIFELSLEEQQKLNVDLLPRDMYEAINEMKKDSLVREALGEHVYNLYLK
HCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
AKFDEWVSYNKQVHDWEIDNYLSKY
EECHHHHHCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]