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The map label for this gene is cbiH [H]

Identifier: 188585563

GI number: 188585563

Start: 993168

End: 993884

Strand: Direct

Name: cbiH [H]

Synonym: Nther_0935

Alternate gene names: 188585563

Gene position: 993168-993884 (Clockwise)

Preceding gene: 188585562

Following gene: 188585564

Centisome position: 31.37

GC content: 39.75

Gene sequence:

>717_bases
GTGGCCGTGGTGGGTATTGGACCCGGTGGTGGAAAGAGCCTATCAATAGAAGCTAAACAAATATTGCTAAACTCGGAAGT
CATTATTGGGTATAAGACTTATATTCAACTGATAGAGGACTTAATTACAGAACAACAGGTAATTTCTTCTTCCATGAAGC
AAGAATTAGACAGATGTCAAGAGGCTCTTAAATTAGCTAAAGAAGGGAAACAAGTAGCCATAGTAAGCAGTGGAGACCCT
GGAGTTTACGGAATGGCAGGGGTTGTATTGGAATTAAATGAAGACCCAGATCTTCCTGTTGAAATTGTTCCGGGAACAAC
AGCCGCTACGGGTGCTGCTTCTTCTTTAGGTGCACCTTTAATGCATGATTTTACAGTTATTAGCCTCAGCGATCTATTGA
CTCCCTGGCAGCAGATCATAAAAAAATTAAAGGCTGTGGCTAGTACTGACTTTGTAGTAGTACTTTATAATCCGAAAAGC
AGTCAACGGAGAGAACAAATTGAAATTGCCCGGGAAATATTTCTGGAATATCGCAGCCCCCAAACCCCTGTTGGATTAGT
TTCTAACAATTACAGACAAGGGGAAAAGACTGTTATTACAACACTGGAGGAATTCACAAACTGTGATATTGATATGAGTA
CAACTGTCGTAATTGGTAATGACCAAAGTTATGTTAAATTCGAAAAGATGATTACTCCAAGGGGTTATCAATTATGA

Upstream 100 bases:

>100_bases
GAATATGCGAACCAGCAGCCAGGACTCTAGCAGGTACAGAGGAACTTTTGATGAAGAAACTGACTCACAAAGGTGTAACT
GTAGCACTGGCCAGGGCAAA

Downstream 100 bases:

>100_bases
TCTTAGTTTTGGGTGGAACCGATGATGGAATTAAAGTTGGCGAATTTTTAACTAAACAAGGTCATTCTGTTATGGTCAGT
GCCAGTACAGATTATGGTAA

Product: precorrin-3 methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MAVVGIGPGGGKSLSIEAKQILLNSEVIIGYKTYIQLIEDLITEQQVISSSMKQELDRCQEALKLAKEGKQVAIVSSGDP
GVYGMAGVVLELNEDPDLPVEIVPGTTAATGAASSLGAPLMHDFTVISLSDLLTPWQQIIKKLKAVASTDFVVVLYNPKS
SQRREQIEIAREIFLEYRSPQTPVGLVSNNYRQGEKTVITTLEEFTNCDIDMSTTVVIGNDQSYVKFEKMITPRGYQL

Sequences:

>Translated_238_residues
MAVVGIGPGGGKSLSIEAKQILLNSEVIIGYKTYIQLIEDLITEQQVISSSMKQELDRCQEALKLAKEGKQVAIVSSGDP
GVYGMAGVVLELNEDPDLPVEIVPGTTAATGAASSLGAPLMHDFTVISLSDLLTPWQQIIKKLKAVASTDFVVVLYNPKS
SQRREQIEIAREIFLEYRSPQTPVGLVSNNYRQGEKTVITTLEEFTNCDIDMSTTVVIGNDQSYVKFEKMITPRGYQL
>Mature_237_residues
AVVGIGPGGGKSLSIEAKQILLNSEVIIGYKTYIQLIEDLITEQQVISSSMKQELDRCQEALKLAKEGKQVAIVSSGDPG
VYGMAGVVLELNEDPDLPVEIVPGTTAATGAASSLGAPLMHDFTVISLSDLLTPWQQIIKKLKAVASTDFVVVLYNPKSS
QRREQIEIAREIFLEYRSPQTPVGLVSNNYRQGEKTVITTLEEFTNCDIDMSTTVVIGNDQSYVKFEKMITPRGYQL

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 25984; Mature: 25853

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVGIGPGGGKSLSIEAKQILLNSEVIIGYKTYIQLIEDLITEQQVISSSMKQELDRCQ
CEEEEECCCCCCEEEEEHHHEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALKLAKEGKQVAIVSSGDPGVYGMAGVVLELNEDPDLPVEIVPGTTAATGAASSLGAPL
HHHHHHHCCCEEEEEECCCCCCEEEEEEEEEECCCCCCCEEEECCCCCCCCCHHHCCCCH
MHDFTVISLSDLLTPWQQIIKKLKAVASTDFVVVLYNPKSSQRREQIEIAREIFLEYRSP
HHHHHEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
QTPVGLVSNNYRQGEKTVITTLEEFTNCDIDMSTTVVIGNDQSYVKFEKMITPRGYQL
CCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEHEEHCCCCCCCC
>Mature Secondary Structure 
AVVGIGPGGGKSLSIEAKQILLNSEVIIGYKTYIQLIEDLITEQQVISSSMKQELDRCQ
EEEEECCCCCCEEEEEHHHEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALKLAKEGKQVAIVSSGDPGVYGMAGVVLELNEDPDLPVEIVPGTTAATGAASSLGAPL
HHHHHHHCCCEEEEEECCCCCCEEEEEEEEEECCCCCCCEEEECCCCCCCCCHHHCCCCH
MHDFTVISLSDLLTPWQQIIKKLKAVASTDFVVVLYNPKSSQRREQIEIAREIFLEYRSP
HHHHHEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
QTPVGLVSNNYRQGEKTVITTLEEFTNCDIDMSTTVVIGNDQSYVKFEKMITPRGYQL
CCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEHEEHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]