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The map label for this gene is purC [H]

Identifier: 188585481

GI number: 188585481

Start: 898231

End: 898911

Strand: Direct

Name: purC [H]

Synonym: Nther_0853

Alternate gene names: 188585481

Gene position: 898231-898911 (Clockwise)

Preceding gene: 188585480

Following gene: 188585484

Centisome position: 28.38

GC content: 34.8

Gene sequence:

>681_bases
ATGAAACAAATTTATGAGGGAAAGACCAAGAAAGTATTTTTGAATGAGAAAGGTAATTTAGTTCTTTACTTTAAAGATGA
GGTAACTGGTACCGGAGAAGATATCGATCCAGGTGGAAATGAAGTGGTGGGTGAAATACCAGAGAAAGGATTATCCTCAC
TGGCAGTAACAAAATATTTTTTTGATATGCTAAAAGAAAAGGGAATTCCAACTCATTTTATCGATGCTTCACCGGAAGAA
GGTTATATGGAAGTACAACAATGTGAAGCTTTAGGCCTGGAAGTTATCTGTCGGGTTAAGGCATATGGTAGTTTCCTGCG
ACGTTATCCTGAGTTTACTCAAAATAAGGAATTGGAACATCTGAACTATTTGGTTGAAGTCACAGTTAAAGATGATGAGA
GGGGTGATCCTTTAATCAATGATGAAGCTTTGATTAAACTAGGGATTTTGAACAATGAAGAGTTAGACTCTATGAAGGAA
ATTACTAAATCTGTTGCCAAAATCATTGAAACTGAGTTAAATGATAAAGGTTTAGATTTAATTGACTTTAAACTGGAATT
TGGTAGGGTTAAAGATGGCAAGATTATCATCATTGACGAAATCTCCGGTGATTCTATGAGGGTCTTCAAAGATGACAAAA
CTGTTACACAAAAAGAACTTGTTGAATATTTGCTGAAATAG

Upstream 100 bases:

>100_bases
TCTTTGATTTTTATTATCACAGCAGGTTTTTGGTATTTTAGTAGGAAAACAGAATTACCTGTTACTATTTTGAGTGACTA
ACTTGAAGGAGGTCAAAAAT

Downstream 100 bases:

>100_bases
AAGATAATTCATTTCCATTATCTTAAATAAATGCATCCTTTCTGTCTTGTCCTTCTCGGTCAGAAAGGATGCATTTATCT
AGTCTAACTGAGGTTATCGA

Product: Phosphoribosylaminoimidazolesuccinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKQIYEGKTKKVFLNEKGNLVLYFKDEVTGTGEDIDPGGNEVVGEIPEKGLSSLAVTKYFFDMLKEKGIPTHFIDASPEE
GYMEVQQCEALGLEVICRVKAYGSFLRRYPEFTQNKELEHLNYLVEVTVKDDERGDPLINDEALIKLGILNNEELDSMKE
ITKSVAKIIETELNDKGLDLIDFKLEFGRVKDGKIIIIDEISGDSMRVFKDDKTVTQKELVEYLLK

Sequences:

>Translated_226_residues
MKQIYEGKTKKVFLNEKGNLVLYFKDEVTGTGEDIDPGGNEVVGEIPEKGLSSLAVTKYFFDMLKEKGIPTHFIDASPEE
GYMEVQQCEALGLEVICRVKAYGSFLRRYPEFTQNKELEHLNYLVEVTVKDDERGDPLINDEALIKLGILNNEELDSMKE
ITKSVAKIIETELNDKGLDLIDFKLEFGRVKDGKIIIIDEISGDSMRVFKDDKTVTQKELVEYLLK
>Mature_226_residues
MKQIYEGKTKKVFLNEKGNLVLYFKDEVTGTGEDIDPGGNEVVGEIPEKGLSSLAVTKYFFDMLKEKGIPTHFIDASPEE
GYMEVQQCEALGLEVICRVKAYGSFLRRYPEFTQNKELEHLNYLVEVTVKDDERGDPLINDEALIKLGILNNEELDSMKE
ITKSVAKIIETELNDKGLDLIDFKLEFGRVKDGKIIIIDEISGDSMRVFKDDKTVTQKELVEYLLK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=231, Percent_Identity=30.7359307359307, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI5453539, Length=231, Percent_Identity=30.7359307359307, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI119220559, Length=238, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1788820, Length=208, Percent_Identity=28.3653846153846, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531275, Length=217, Percent_Identity=31.3364055299539, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583917, Length=216, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI18860083, Length=222, Percent_Identity=30.1801801801802, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 25711; Mature: 25711

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS01057 SAICAR_SYNTHETASE_1 ; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIYEGKTKKVFLNEKGNLVLYFKDEVTGTGEDIDPGGNEVVGEIPEKGLSSLAVTKYF
CCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
FDMLKEKGIPTHFIDASPEEGYMEVQQCEALGLEVICRVKAYGSFLRRYPEFTQNKELEH
HHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LNYLVEVTVKDDERGDPLINDEALIKLGILNNEELDSMKEITKSVAKIIETELNDKGLDL
EEEEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IDFKLEFGRVKDGKIIIIDEISGDSMRVFKDDKTVTQKELVEYLLK
EEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHC
>Mature Secondary Structure
MKQIYEGKTKKVFLNEKGNLVLYFKDEVTGTGEDIDPGGNEVVGEIPEKGLSSLAVTKYF
CCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
FDMLKEKGIPTHFIDASPEEGYMEVQQCEALGLEVICRVKAYGSFLRRYPEFTQNKELEH
HHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LNYLVEVTVKDDERGDPLINDEALIKLGILNNEELDSMKEITKSVAKIIETELNDKGLDL
EEEEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IDFKLEFGRVKDGKIIIIDEISGDSMRVFKDDKTVTQKELVEYLLK
EEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]