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The map label for this gene is rpfG [H]

Identifier: 188585413

GI number: 188585413

Start: 815408

End: 816859

Strand: Reverse

Name: rpfG [H]

Synonym: Nther_0783

Alternate gene names: 188585413

Gene position: 816859-815408 (Counterclockwise)

Preceding gene: 188585414

Following gene: 188585412

Centisome position: 25.8

GC content: 36.29

Gene sequence:

>1452_bases
ATGACCATTGAACACAAACAAGCAGAAGAAGAGCTACGTCTTAGTGAAGAGAAATTCAGGCGATTTATCGAAAATGCCAG
TGATATAGTCTTTACTTTGGACAAGGAAGGGTATTTAACCTTTGTTTCCCCTAACTGGAAACAATTATTGGGCCATGATC
CGGAGAATGTTGTAGGTAACCATTATAAAGAATTTGTACATAGTGAAGATATTGAAAAAGTCATAGCTAAACATAAAGAA
CTTTTAGAAACCGGTACTTTAGATAGCGAAATAGAGTACAGGGTTAGACATAAAAGTGGCGAGTGGAAATGGCATGCCTC
TCGCATAAATATAATGGAAAGTGACGAAAATCGGGTAGTGATACTTGGTGTAGCTCGAGATATCACCTTAAGAAAAGAAG
CTGAAAGTAAGATACGTTATTTAAGCTTTCATGACAGCTTGACTGGTTTATACAATAGAGCCTATTTAGATGAACAAATC
AAGCAATTAAATAATAAACACTTCTATCCCTTAAGTGTTATCATGGCTGATTTAAACGGACTAAAATTAGTTAACGATAC
TTATGGTCATTTAGCCGGGGACGAAATGCTTAAAAAAGCTGCCAGCTCAATTAAAAATTCCATTCGTAAGAATGATATCG
TTGCACGCTGGGGTGGTGATGAATTCTTGATACTTTTACCCCAGACCAATAAACAAGATGCAAAAAAACTAAGCGAGCGG
CTCTTAGATAGTTGCAAAAATCAATATGTTCAAGATGTTCCCATCTCATTAGCTGTAGGAAGAGCAACAATAACCAAACC
TGAAAATGATGATGAATATTTAACTAAAACACTAATTGAAGCTGAAGATGATATGTATAAACAAAAATTAGCAAACAGTA
GAAGTGCAAGAAGTTCTATTTTAACCGCCTTGTTAAAAACCTTAGAAGCAAAAAGTTTTGAAACAGAAAGTCATACTGTA
CGTATGCGCAATATTGCTAAACAAATAGGTAAAAAAATCGGATTATCTGATTCGGAATTAAACAAGCTAAATCTATTAAT
TACTCTCCATGACATAGGTAAGATTAACATCCCGGAAGATATCTTGAGTAAACAGGGAAAGTTAACAGAAAAAGAATGGG
AAACTATCAAAACTCACCCCCTCACCGGTTATCGCATAGCCCTTGCTACAGAAGAGTTTGCCCATGTGGCAGAGGGAATT
TTATCTCACCATGAGCGATGGGACGGTAAGGGGTATCCTCAAGGACTAAAGGAAAAAGAAATCCCTCTATTGGCAAGGAT
CACTACCATTGCCGATGCTTATGAAGTGATGACAAATGGAAGACCTTATCAAAAACCCATGACCCAAGGGGAAATTATTG
AAGAGTTTAAACGTTGTTCAGGTTCTCAATTTGATCCAGAATTAGTACAAGCTTTCCTAGAAATATTGACTAATACAGAG
ATCCAACTTTAA

Upstream 100 bases:

>100_bases
AAGGTGAAATCATTGCGACTTTCGTTGTTTATCATAACAAGCCTAAATCCCCTGATGAAAAAGATCTGAAAGACTTAAAA
TTTCTGCTGACTCAACCAGG

Downstream 100 bases:

>100_bases
CGAGATAAATTTTTGGATAATTTTCTGAAAAAAGATTGTGCTTTCATTTCTTTCATGGTATAATAAACATGTAACACGTT
AGTGTAACACAATTAAACTT

Product: diguanylate cyclase and metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTIEHKQAEEELRLSEEKFRRFIENASDIVFTLDKEGYLTFVSPNWKQLLGHDPENVVGNHYKEFVHSEDIEKVIAKHKE
LLETGTLDSEIEYRVRHKSGEWKWHASRINIMESDENRVVILGVARDITLRKEAESKIRYLSFHDSLTGLYNRAYLDEQI
KQLNNKHFYPLSVIMADLNGLKLVNDTYGHLAGDEMLKKAASSIKNSIRKNDIVARWGGDEFLILLPQTNKQDAKKLSER
LLDSCKNQYVQDVPISLAVGRATITKPENDDEYLTKTLIEAEDDMYKQKLANSRSARSSILTALLKTLEAKSFETESHTV
RMRNIAKQIGKKIGLSDSELNKLNLLITLHDIGKINIPEDILSKQGKLTEKEWETIKTHPLTGYRIALATEEFAHVAEGI
LSHHERWDGKGYPQGLKEKEIPLLARITTIADAYEVMTNGRPYQKPMTQGEIIEEFKRCSGSQFDPELVQAFLEILTNTE
IQL

Sequences:

>Translated_483_residues
MTIEHKQAEEELRLSEEKFRRFIENASDIVFTLDKEGYLTFVSPNWKQLLGHDPENVVGNHYKEFVHSEDIEKVIAKHKE
LLETGTLDSEIEYRVRHKSGEWKWHASRINIMESDENRVVILGVARDITLRKEAESKIRYLSFHDSLTGLYNRAYLDEQI
KQLNNKHFYPLSVIMADLNGLKLVNDTYGHLAGDEMLKKAASSIKNSIRKNDIVARWGGDEFLILLPQTNKQDAKKLSER
LLDSCKNQYVQDVPISLAVGRATITKPENDDEYLTKTLIEAEDDMYKQKLANSRSARSSILTALLKTLEAKSFETESHTV
RMRNIAKQIGKKIGLSDSELNKLNLLITLHDIGKINIPEDILSKQGKLTEKEWETIKTHPLTGYRIALATEEFAHVAEGI
LSHHERWDGKGYPQGLKEKEIPLLARITTIADAYEVMTNGRPYQKPMTQGEIIEEFKRCSGSQFDPELVQAFLEILTNTE
IQL
>Mature_482_residues
TIEHKQAEEELRLSEEKFRRFIENASDIVFTLDKEGYLTFVSPNWKQLLGHDPENVVGNHYKEFVHSEDIEKVIAKHKEL
LETGTLDSEIEYRVRHKSGEWKWHASRINIMESDENRVVILGVARDITLRKEAESKIRYLSFHDSLTGLYNRAYLDEQIK
QLNNKHFYPLSVIMADLNGLKLVNDTYGHLAGDEMLKKAASSIKNSIRKNDIVARWGGDEFLILLPQTNKQDAKKLSERL
LDSCKNQYVQDVPISLAVGRATITKPENDDEYLTKTLIEAEDDMYKQKLANSRSARSSILTALLKTLEAKSFETESHTVR
MRNIAKQIGKKIGLSDSELNKLNLLITLHDIGKINIPEDILSKQGKLTEKEWETIKTHPLTGYRIALATEEFAHVAEGIL
SHHERWDGKGYPQGLKEKEIPLLARITTIADAYEVMTNGRPYQKPMTQGEIIEEFKRCSGSQFDPELVQAFLEILTNTEI
QL

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=297, Percent_Identity=25.5892255892256, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87082007, Length=110, Percent_Identity=40, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=190, Percent_Identity=31.0526315789474, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=31.1111111111111, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI145693134, Length=148, Percent_Identity=33.1081081081081, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787262, Length=98, Percent_Identity=39.7959183673469, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787816, Length=152, Percent_Identity=32.2368421052632, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081974, Length=100, Percent_Identity=43, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788085, Length=193, Percent_Identity=29.0155440414508, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787802, Length=155, Percent_Identity=30.3225806451613, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=26.3473053892216, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=164, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 55374; Mature: 55243

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEHKQAEEELRLSEEKFRRFIENASDIVFTLDKEGYLTFVSPNWKQLLGHDPENVVGN
CCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEEEECCCHHHHHCCCCHHHHHH
HYKEFVHSEDIEKVIAKHKELLETGTLDSEIEYRVRHKSGEWKWHASRINIMESDENRVV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCEEEECCEEEEEECCCCEEE
ILGVARDITLRKEAESKIRYLSFHDSLTGLYNRAYLDEQIKQLNNKHFYPLSVIMADLNG
EEEECCCCCHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
LKLVNDTYGHLAGDEMLKKAASSIKNSIRKNDIVARWGGDEFLILLPQTNKQDAKKLSER
CEEECCHHHHCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCHHHHHHHHHH
LLDSCKNQYVQDVPISLAVGRATITKPENDDEYLTKTLIEAEDDMYKQKLANSRSARSSI
HHHHHHHHHHHCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LTALLKTLEAKSFETESHTVRMRNIAKQIGKKIGLSDSELNKLNLLITLHDIGKINIPED
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCHH
ILSKQGKLTEKEWETIKTHPLTGYRIALATEEFAHVAEGILSHHERWDGKGYPQGLKEKE
HHHCCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
IPLLARITTIADAYEVMTNGRPYQKPMTQGEIIEEFKRCSGSQFDPELVQAFLEILTNTE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
IQL
CCC
>Mature Secondary Structure 
TIEHKQAEEELRLSEEKFRRFIENASDIVFTLDKEGYLTFVSPNWKQLLGHDPENVVGN
CCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEEEECCCHHHHHCCCCHHHHHH
HYKEFVHSEDIEKVIAKHKELLETGTLDSEIEYRVRHKSGEWKWHASRINIMESDENRVV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCEEEECCEEEEEECCCCEEE
ILGVARDITLRKEAESKIRYLSFHDSLTGLYNRAYLDEQIKQLNNKHFYPLSVIMADLNG
EEEECCCCCHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
LKLVNDTYGHLAGDEMLKKAASSIKNSIRKNDIVARWGGDEFLILLPQTNKQDAKKLSER
CEEECCHHHHCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCHHHHHHHHHH
LLDSCKNQYVQDVPISLAVGRATITKPENDDEYLTKTLIEAEDDMYKQKLANSRSARSSI
HHHHHHHHHHHCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LTALLKTLEAKSFETESHTVRMRNIAKQIGKKIGLSDSELNKLNLLITLHDIGKINIPED
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCHH
ILSKQGKLTEKEWETIKTHPLTGYRIALATEEFAHVAEGILSHHERWDGKGYPQGLKEKE
HHHCCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
IPLLARITTIADAYEVMTNGRPYQKPMTQGEIIEEFKRCSGSQFDPELVQAFLEILTNTE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
IQL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]