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The map label for this gene is opuE [H]

Identifier: 188585399

GI number: 188585399

Start: 797318

End: 798781

Strand: Direct

Name: opuE [H]

Synonym: Nther_0769

Alternate gene names: 188585399

Gene position: 797318-798781 (Clockwise)

Preceding gene: 188585398

Following gene: 188585400

Centisome position: 25.19

GC content: 35.52

Gene sequence:

>1464_bases
TTGGATATTTATAGTATAGTTGCTGTCCTGTTTCTTGTATTATACTTATTTGTAGGTGTTTATGCCGGGCGGGTTACAAA
TACCACTGGTGATCATATGGTTATGAGCAAAAGTGCTGTGGGGTATCAAATAACAGGATCGCTAATTGCTTCTAACTTGA
GTAGTGTAACTTTAATAGGTTATACAGCTACAGTTGTAGCAAGTGGTCCTCTAGCTATACTTTCTCAATTTGGGATGACT
GTTGTTGCTAGCTTATTTATTGGACTATTAATTGGTAGATATATTAGAAGGATGGATTTAAATACAATCCCTGAATTTTT
TGAGAAAAGATATCCTAGTCAAGCTATACAATTAGTTTCCTCAATCATATTATTAATTTCTATGGTAGCTTATACAATCA
GCGTTACAATAGGTACAGTTGTTGTCGGTAAAAATTTATTTGGCTGGAATGAGATGACCTCTCTAATAGCTGTGATCCTT
TTGATTACTATGTTTACTATTAGAGGTGGAATGCGTTCTGTTGTTATTACGGATGTAATAATGTTTTGTGTGTTTTTTAT
GTCTTCTTTATTGATAGGGTATCCAATAATTCAGCACCTTGGAGGTTTTGAGTTAGCAATTCAAAAAGCAACACCAGAGT
TTACACATCTTTTTAATTGGGTTGGAGACGACTCCCTTCTCCAGGGTACCATGAATATCATTGAATTAAATGTTTTATGT
TTAATTAATGTTATGGCCGCACCTCATTTGATGAGTAGGATCTATATTGCGAAAAGTGAACGTGAGTTTGGTAAAGCTAT
GCTATATCTTTCAATAGGGATACCAATTACGGTATTTGGGTTGCTATACTCTTTTGGATATTTACCAGTATTAGAACATG
TTCAAGGGTTTACTGTTGAAGGAGAGGTATTTCCGTGGGTTGCAAGGAATTTAGTAAATCCTTTTGTAGGGGCAATAGGT
ATGTCTGGGGTTATAGCAGCGGCAATTTCTACTTCATCGTCTCTTTTGCAACAAGCTTCCGCTACCCTTGCGAATGATAT
AATTAAAAAATACTTTATAAAAGATTTATCAGATAATAGAGTTCTTCTGGTTACCAGGGTTTGTGTTGTAGTTCTAGCTA
TAATAGTTTTTATTGGTGCATCAATACCAACTATTGAAGGTTTAACTGTTCTGTATGCATTCTTGTTTGCTACCTCAGTT
TTTGCTGCTTGGTGGCCTTCAATAATAATTGGAACGTTGTGGAAGAAAGGGTCTACTTCTGGGGCTTTTTGGTCAATGAG
TATGTCACTATTTGTTGCAGTTATTTTAGGCTTTACTAGAGCTGCCGGGTTAACGCCAGAATGGGTCGTGCCAAATGTTG
CTGCACTAATTACATCAACAACGCTATTTATAGTCATATCATTGTTTACAGAGAATCAAAAAGAGGAGTTAGAATTAATG
GACAAAATTAGTCGTTCAGTTTAA

Upstream 100 bases:

>100_bases
ACATCTTTGAAATCCAGCGAATGGTGTTTTACAAACTGTAAATGCAGTTGTACTAGATATGAATTTTGTAAGAAATAAAT
GCAGTTAAGGAGGAAATAAA

Downstream 100 bases:

>100_bases
CTATTTCAAGTTACGACAGTTTATTACTAATCAAAGAGTAGGAGGTTGAAAATGAATTCTTCCAATTTACCTGTTGGTTT
AATTTGTTCTGGAGTATTAT

Product: Na+/solute symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MDIYSIVAVLFLVLYLFVGVYAGRVTNTTGDHMVMSKSAVGYQITGSLIASNLSSVTLIGYTATVVASGPLAILSQFGMT
VVASLFIGLLIGRYIRRMDLNTIPEFFEKRYPSQAIQLVSSIILLISMVAYTISVTIGTVVVGKNLFGWNEMTSLIAVIL
LITMFTIRGGMRSVVITDVIMFCVFFMSSLLIGYPIIQHLGGFELAIQKATPEFTHLFNWVGDDSLLQGTMNIIELNVLC
LINVMAAPHLMSRIYIAKSEREFGKAMLYLSIGIPITVFGLLYSFGYLPVLEHVQGFTVEGEVFPWVARNLVNPFVGAIG
MSGVIAAAISTSSSLLQQASATLANDIIKKYFIKDLSDNRVLLVTRVCVVVLAIIVFIGASIPTIEGLTVLYAFLFATSV
FAAWWPSIIIGTLWKKGSTSGAFWSMSMSLFVAVILGFTRAAGLTPEWVVPNVAALITSTTLFIVISLFTENQKEELELM
DKISRSV

Sequences:

>Translated_487_residues
MDIYSIVAVLFLVLYLFVGVYAGRVTNTTGDHMVMSKSAVGYQITGSLIASNLSSVTLIGYTATVVASGPLAILSQFGMT
VVASLFIGLLIGRYIRRMDLNTIPEFFEKRYPSQAIQLVSSIILLISMVAYTISVTIGTVVVGKNLFGWNEMTSLIAVIL
LITMFTIRGGMRSVVITDVIMFCVFFMSSLLIGYPIIQHLGGFELAIQKATPEFTHLFNWVGDDSLLQGTMNIIELNVLC
LINVMAAPHLMSRIYIAKSEREFGKAMLYLSIGIPITVFGLLYSFGYLPVLEHVQGFTVEGEVFPWVARNLVNPFVGAIG
MSGVIAAAISTSSSLLQQASATLANDIIKKYFIKDLSDNRVLLVTRVCVVVLAIIVFIGASIPTIEGLTVLYAFLFATSV
FAAWWPSIIIGTLWKKGSTSGAFWSMSMSLFVAVILGFTRAAGLTPEWVVPNVAALITSTTLFIVISLFTENQKEELELM
DKISRSV
>Mature_487_residues
MDIYSIVAVLFLVLYLFVGVYAGRVTNTTGDHMVMSKSAVGYQITGSLIASNLSSVTLIGYTATVVASGPLAILSQFGMT
VVASLFIGLLIGRYIRRMDLNTIPEFFEKRYPSQAIQLVSSIILLISMVAYTISVTIGTVVVGKNLFGWNEMTSLIAVIL
LITMFTIRGGMRSVVITDVIMFCVFFMSSLLIGYPIIQHLGGFELAIQKATPEFTHLFNWVGDDSLLQGTMNIIELNVLC
LINVMAAPHLMSRIYIAKSEREFGKAMLYLSIGIPITVFGLLYSFGYLPVLEHVQGFTVEGEVFPWVARNLVNPFVGAIG
MSGVIAAAISTSSSLLQQASATLANDIIKKYFIKDLSDNRVLLVTRVCVVVLAIIVFIGASIPTIEGLTVLYAFLFATSV
FAAWWPSIIIGTLWKKGSTSGAFWSMSMSLFVAVILGFTRAAGLTPEWVVPNVAALITSTTLFIVISLFTENQKEELELM
DKISRSV

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=523, Percent_Identity=25.8126195028681, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4507033, Length=213, Percent_Identity=29.1079812206573, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1787251, Length=437, Percent_Identity=24.9427917620137, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790503, Length=443, Percent_Identity=20.3160270880361, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082237, Length=436, Percent_Identity=22.9357798165138, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53108; Mature: 53108

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIYSIVAVLFLVLYLFVGVYAGRVTNTTGDHMVMSKSAVGYQITGSLIASNLSSVTLIG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEHHHHHHCCCCCEEEEE
YTATVVASGPLAILSQFGMTVVASLFIGLLIGRYIRRMDLNTIPEFFEKRYPSQAIQLVS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
SIILLISMVAYTISVTIGTVVVGKNLFGWNEMTSLIAVILLITMFTIRGGMRSVVITDVI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MFCVFFMSSLLIGYPIIQHLGGFELAIQKATPEFTHLFNWVGDDSLLQGTMNIIELNVLC
HHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LINVMAAPHLMSRIYIAKSEREFGKAMLYLSIGIPITVFGLLYSFGYLPVLEHVQGFTVE
HHHHHHHHHHHHHHHHHCCHHHHHHEEEEEECCCHHHHHHHHHHHCHHHHHHHHCCCCCC
GEVFPWVARNLVNPFVGAIGMSGVIAAAISTSSSLLQQASATLANDIIKKYFIKDLSDNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VLLVTRVCVVVLAIIVFIGASIPTIEGLTVLYAFLFATSVFAAWWPSIIIGTLWKKGSTS
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAFWSMSMSLFVAVILGFTRAAGLTPEWVVPNVAALITSTTLFIVISLFTENQKEELELM
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
DKISRSV
HHHHHCC
>Mature Secondary Structure
MDIYSIVAVLFLVLYLFVGVYAGRVTNTTGDHMVMSKSAVGYQITGSLIASNLSSVTLIG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEHHHHHHCCCCCEEEEE
YTATVVASGPLAILSQFGMTVVASLFIGLLIGRYIRRMDLNTIPEFFEKRYPSQAIQLVS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
SIILLISMVAYTISVTIGTVVVGKNLFGWNEMTSLIAVILLITMFTIRGGMRSVVITDVI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MFCVFFMSSLLIGYPIIQHLGGFELAIQKATPEFTHLFNWVGDDSLLQGTMNIIELNVLC
HHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LINVMAAPHLMSRIYIAKSEREFGKAMLYLSIGIPITVFGLLYSFGYLPVLEHVQGFTVE
HHHHHHHHHHHHHHHHHCCHHHHHHEEEEEECCCHHHHHHHHHHHCHHHHHHHHCCCCCC
GEVFPWVARNLVNPFVGAIGMSGVIAAAISTSSSLLQQASATLANDIIKKYFIKDLSDNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VLLVTRVCVVVLAIIVFIGASIPTIEGLTVLYAFLFATSVFAAWWPSIIIGTLWKKGSTS
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAFWSMSMSLFVAVILGFTRAAGLTPEWVVPNVAALITSTTLFIVISLFTENQKEELELM
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
DKISRSV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]