Click here to switch to the map view.

The map label for this gene is cofE [H]

Identifier: 188585266

GI number: 188585266

Start: 652115

End: 653029

Strand: Direct

Name: cofE [H]

Synonym: Nther_0628

Alternate gene names: 188585266

Gene position: 652115-653029 (Clockwise)

Preceding gene: 188585265

Following gene: 188585267

Centisome position: 20.6

GC content: 38.47

Gene sequence:

>915_bases
GTGAAAGTTGCGAAATTAATTGGTCTAGACAAAACACCTGATATTAAACCAGGTCACGATTTAGCGCAAATTATATCAGA
AAATATGGTAAAAGAAAGTTTAGAATTAGTTTCTGGAGACATAGTTGTAATCAGCAGTAAAATAGTTTCCAAGGCAGAAA
ATCGAATAATAGATCTCAACTATGTTTCACCTGGTAAAAAGGCTAAATCCCTTTCCCAATTGACGGGGAAAGATGCTGCC
CGAATTCAAGTGATCCTTGAAGAAGCAGAGCAAGTGGTTGGTTATCTGTCACTAAAAAAGCTTGTTCAGGACCGAGGTTT
TGTTGAATCTTATTTTGGGAATGAAGATGCCCACAAAATGTTACAGAATAATGAAGCTGTACTCTTTACCGTTATGCCCA
GTGGACAATTGATGACAGATGGAGGAACAGATTTTTCCAATGTAAAAGGAAAAAGAGTATTGTCTTTGCTCCCTCGCAAG
CCCATGGAAAGCGCTGAGGAAATTCGAACACAACTTGAAAGCATTTGGAATTGTTCACTAGGGGTGGTGGTTTCTGATTC
AGAGATTAGAGCTCTTCGTCAAGGAACTGCAGATATAGCAATTGGTTATAGTGGGATAACTCCTTTAGAGCAAGGCTTTG
GCAAACAAGATTTATATGGTGAACCTACATTTGGTGGGGTTGATTCCATCTGTGACCAAATAGCAAATAGTGCGGCTTTG
TTGATGGGACAGCGGGATGAGAATGTACCGGTTGTCATACTTAAAGGACTGGCCAATTTTCAAGAAAATTATGTTAAAAT
AGATAGCAAACCAGAATTACCATATAGTTACATGGCCAGATTCATTAAAGAAGTTGCCATATCCAGGTTCAAATTATATA
CTTGGCCTTTACTAGCTTTGTTGAGAAATGACTAG

Upstream 100 bases:

>100_bases
TTATAACTGAGCAAGTGGATATTGCTATAAAGACAGGATACCTCCTGGCTATTAAGGTTAACACAAAATTTTAATTTGAG
ACAACAACTGGAGGGGTATC

Downstream 100 bases:

>100_bases
TAGGTAGGGAGAGTAACTATTTTTCACATAAAAAATTAAAACAGGAATATAAAGACACTAAGGGAGGACGAATTCATGGA
AAAAAAAGTAGTTGGAGTAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKVAKLIGLDKTPDIKPGHDLAQIISENMVKESLELVSGDIVVISSKIVSKAENRIIDLNYVSPGKKAKSLSQLTGKDAA
RIQVILEEAEQVVGYLSLKKLVQDRGFVESYFGNEDAHKMLQNNEAVLFTVMPSGQLMTDGGTDFSNVKGKRVLSLLPRK
PMESAEEIRTQLESIWNCSLGVVVSDSEIRALRQGTADIAIGYSGITPLEQGFGKQDLYGEPTFGGVDSICDQIANSAAL
LMGQRDENVPVVILKGLANFQENYVKIDSKPELPYSYMARFIKEVAISRFKLYTWPLLALLRND

Sequences:

>Translated_304_residues
MKVAKLIGLDKTPDIKPGHDLAQIISENMVKESLELVSGDIVVISSKIVSKAENRIIDLNYVSPGKKAKSLSQLTGKDAA
RIQVILEEAEQVVGYLSLKKLVQDRGFVESYFGNEDAHKMLQNNEAVLFTVMPSGQLMTDGGTDFSNVKGKRVLSLLPRK
PMESAEEIRTQLESIWNCSLGVVVSDSEIRALRQGTADIAIGYSGITPLEQGFGKQDLYGEPTFGGVDSICDQIANSAAL
LMGQRDENVPVVILKGLANFQENYVKIDSKPELPYSYMARFIKEVAISRFKLYTWPLLALLRND
>Mature_304_residues
MKVAKLIGLDKTPDIKPGHDLAQIISENMVKESLELVSGDIVVISSKIVSKAENRIIDLNYVSPGKKAKSLSQLTGKDAA
RIQVILEEAEQVVGYLSLKKLVQDRGFVESYFGNEDAHKMLQNNEAVLFTVMPSGQLMTDGGTDFSNVKGKRVLSLLPRK
PMESAEEIRTQLESIWNCSLGVVVSDSEIRALRQGTADIAIGYSGITPLEQGFGKQDLYGEPTFGGVDSICDQIANSAAL
LMGQRDENVPVVILKGLANFQENYVKIDSKPELPYSYMARFIKEVAISRFKLYTWPLLALLRND

Specific function: Catalyzes the GTP-dependent successive addition of two L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8- hydroxy-5-deazariboflavin (F420-0) to form F420-0-glutamyl- glutamate (F420-2), with a gamma-linkage between the two glutamates. Cannot

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 33499; Mature: 33499

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAKLIGLDKTPDIKPGHDLAQIISENMVKESLELVSGDIVVISSKIVSKAENRIIDLN
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCEEEEE
YVSPGKKAKSLSQLTGKDAARIQVILEEAEQVVGYLSLKKLVQDRGFVESYFGNEDAHKM
ECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHH
LQNNEAVLFTVMPSGQLMTDGGTDFSNVKGKRVLSLLPRKPMESAEEIRTQLESIWNCSL
HHCCCEEEEEEECCCCEEECCCCCHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
GVVVSDSEIRALRQGTADIAIGYSGITPLEQGFGKQDLYGEPTFGGVDSICDQIANSAAL
EEEECCHHHHHHHCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEE
LMGQRDENVPVVILKGLANFQENYVKIDSKPELPYSYMARFIKEVAISRFKLYTWPLLAL
EECCCCCCCCEEEEHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRND
HCCC
>Mature Secondary Structure
MKVAKLIGLDKTPDIKPGHDLAQIISENMVKESLELVSGDIVVISSKIVSKAENRIIDLN
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCEEEEE
YVSPGKKAKSLSQLTGKDAARIQVILEEAEQVVGYLSLKKLVQDRGFVESYFGNEDAHKM
ECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHH
LQNNEAVLFTVMPSGQLMTDGGTDFSNVKGKRVLSLLPRKPMESAEEIRTQLESIWNCSL
HHCCCEEEEEEECCCCEEECCCCCHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
GVVVSDSEIRALRQGTADIAIGYSGITPLEQGFGKQDLYGEPTFGGVDSICDQIANSAAL
EEEECCHHHHHHHCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEE
LMGQRDENVPVVILKGLANFQENYVKIDSKPELPYSYMARFIKEVAISRFKLYTWPLLAL
EECCCCCCCCEEEEHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRND
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]