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The map label for this gene is thiN [H]

Identifier: 188585265

GI number: 188585265

Start: 651429

End: 652088

Strand: Direct

Name: thiN [H]

Synonym: Nther_0627

Alternate gene names: 188585265

Gene position: 651429-652088 (Clockwise)

Preceding gene: 188585262

Following gene: 188585266

Centisome position: 20.58

GC content: 34.09

Gene sequence:

>660_bases
TTGGTTTCGGGAAAAATTAATCGGGTAGCTATTTTTGCCAATGGAGACTACGATAATCCTCAGTTTTATTGTGAAGAACT
GGAGAAAAGTGATTACATAATAGCGGTTGATGGTGGAGCTAACTTTTTATATAAAGTCGATGAAACTCCTGATCTTTTGC
TTGGTGATATGGATTCGATAACAGCTGAAGCTTATCAGTATTACTCCAGATTAGATATCCCGATTAAACACCATCCTGCT
GAAAAGGATGAATCTGATTTAGAACTAGCTTTAGTTACAGGAATAAATTTAAAACCCAAAGAAATATTTGTATATGGTGC
CTTTGGTAACAGGGTTGATCACTTATTTGCAAATATTATGGTTATGTTGAATCCCATAAAAAATGGGATCTATACTTGTT
TAAAAGATACATGCCATGAAATCACTATTACTGATTCCAAATTAACTTTGATTGGAAATCCCGGTGATTACTTGTCTTTA
TTTGCTTTAACTCAGGAAGTGAGTGGTATTTACGCTCATGGGGTAAAATATCCTTTAGAAAACGATTCTTTAACTATGGG
TCCTTCTAGAGGCTTAAGCAATGAATTTATAACTGAGCAAGTGGATATTGCTATAAAGACAGGATACCTCCTGGCTATTA
AGGTTAACACAAAATTTTAA

Upstream 100 bases:

>100_bases
AATTTCAATTTTACTTTACAATGCTTATACTTGGTACATCTAATACTCCTAAACAAAGGATTTAGAGCGCCTTGTAATAA
GAGGAGAGGGGGAAACTTAA

Downstream 100 bases:

>100_bases
TTTGAGACAACAACTGGAGGGGTATCGTGAAAGTTGCGAAATTAATTGGTCTAGACAAAACACCTGATATTAAACCAGGT
CACGATTTAGCGCAAATTAT

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MVSGKINRVAIFANGDYDNPQFYCEELEKSDYIIAVDGGANFLYKVDETPDLLLGDMDSITAEAYQYYSRLDIPIKHHPA
EKDESDLELALVTGINLKPKEIFVYGAFGNRVDHLFANIMVMLNPIKNGIYTCLKDTCHEITITDSKLTLIGNPGDYLSL
FALTQEVSGIYAHGVKYPLENDSLTMGPSRGLSNEFITEQVDIAIKTGYLLAIKVNTKF

Sequences:

>Translated_219_residues
MVSGKINRVAIFANGDYDNPQFYCEELEKSDYIIAVDGGANFLYKVDETPDLLLGDMDSITAEAYQYYSRLDIPIKHHPA
EKDESDLELALVTGINLKPKEIFVYGAFGNRVDHLFANIMVMLNPIKNGIYTCLKDTCHEITITDSKLTLIGNPGDYLSL
FALTQEVSGIYAHGVKYPLENDSLTMGPSRGLSNEFITEQVDIAIKTGYLLAIKVNTKF
>Mature_219_residues
MVSGKINRVAIFANGDYDNPQFYCEELEKSDYIIAVDGGANFLYKVDETPDLLLGDMDSITAEAYQYYSRLDIPIKHHPA
EKDESDLELALVTGINLKPKEIFVYGAFGNRVDHLFANIMVMLNPIKNGIYTCLKDTCHEITITDSKLTLIGNPGDYLSL
FALTQEVSGIYAHGVKYPLENDSLTMGPSRGLSNEFITEQVDIAIKTGYLLAIKVNTKF

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 24404; Mature: 24404

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSGKINRVAIFANGDYDNPQFYCEELEKSDYIIAVDGGANFLYKVDETPDLLLGDMDSI
CCCCCEEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCEEECCCHHH
TAEAYQYYSRLDIPIKHHPAEKDESDLELALVTGINLKPKEIFVYGAFGNRVDHLFANIM
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
VMLNPIKNGIYTCLKDTCHEITITDSKLTLIGNPGDYLSLFALTQEVSGIYAHGVKYPLE
HEEEHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCCEECCEECCCC
NDSLTMGPSRGLSNEFITEQVDIAIKTGYLLAIKVNTKF
CCCEEECCCCCCCCCCCCCEEEEEEECCEEEEEEEECCC
>Mature Secondary Structure
MVSGKINRVAIFANGDYDNPQFYCEELEKSDYIIAVDGGANFLYKVDETPDLLLGDMDSI
CCCCCEEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCEEECCCHHH
TAEAYQYYSRLDIPIKHHPAEKDESDLELALVTGINLKPKEIFVYGAFGNRVDHLFANIM
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
VMLNPIKNGIYTCLKDTCHEITITDSKLTLIGNPGDYLSLFALTQEVSGIYAHGVKYPLE
HEEEHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCCEECCEECCCC
NDSLTMGPSRGLSNEFITEQVDIAIKTGYLLAIKVNTKF
CCCEEECCCCCCCCCCCCCEEEEEEECCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]