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The map label for this gene is cpsA1 [H]

Identifier: 188585251

GI number: 188585251

Start: 639502

End: 640704

Strand: Direct

Name: cpsA1 [H]

Synonym: Nther_0613

Alternate gene names: 188585251

Gene position: 639502-640704 (Clockwise)

Preceding gene: 188585250

Following gene: 188585253

Centisome position: 20.2

GC content: 39.48

Gene sequence:

>1203_bases
TTGTTAAAAGAAATCACGGACAGTATCAAAGAGATCGAAAATTGGATAATTGATAAAAGGAGACAGTTACACAAATATCC
TGAAAAGAGCGCCTGTGAAGTTAAAACTAAAGAAGTTCTAAAGTCTACTTTAGAAGATTTAGGGATTGAGGTAGTCGAAG
GGTATTATACTACTGGTCTTACTGCTGTCATTAGAGGTAAATTAAGTGGTTCAAAGGATAAGACAATTGGCCTCAGATTT
GACATGGATGCTTTGGAAATGGAAGAAAAAACGGAGTTGGATTTTAAATCACAAAATCCTGGTCTAATGCATGCTTGCGG
TCACGATGGGCATATGGCTATGGGACTTGGCTGTGCCGTAGTTTTAAATAAGTTTAGAGATAAATTTGCTGGGAATATAA
AATTAATTTTTCAACCTGCCGAGGAAGATGCATTAAACGGTGGTGGAGCTAGATACATGATTGAAGATGGTGTTTTGCAT
GATGAACCTGGAGTTGATGCCATGGTGGGAGTTCATATTTGGCCCACTTTAAATGTTGGTACGGCTGGGACTAGAGTAGG
GCCCATCATGGCAGCATCAGATCCCTTTAAAATTAGAGTTAAAGGTAAAGGTGTGCACGCTTCTCTACCGCATATGGGTA
CCGATCCTATTCTAATTGCTTCTCAGATAGTAACTAATTTACAAAGTATTGTTAGTCGAAATATCGATCCCTTTGAACAG
GCTGTTGTCTCTACAGGGACAATCCAAGGTGGGACGGCACATAATACAATTCCTGATGAAGTTGAAATAATGGGGACTGT
GAGAACTTTTGACGATAATATCAGACAAGTAGTAAAGGAAAAGATGCAGGAAATAGTGACTAAAACTGCAGAATCCCTGG
GCGGTCAAGGTGAGTTGGAGTATACTTTTGGTTATCCACCTACCGTAAATAACGAAAAAATGGTTTGTGTTGCGCAAAAA
GCTATTAAGGCTGTTTTAGGAGACGAAAACTACATTCCGGTTCAGCGACCAGCTCCGGGTGGAGAAGACTTTGCTTACTT
TGCCCGAGAGGTACCCTCAGCCTTTATTTGGCTAGGCTATAATCAGGAAAATGAGCAAATTTTTCCACCCCACAATCCAT
ACTATAATTTTAATGAAGGTATATTAATCTGGGGAACGGAAATTTACTGTAACATAGCCTTAGAATGGCTCAGAAATAAT
TAA

Upstream 100 bases:

>100_bases
GAGTGAAGAAAGATCCTAAAAACTTTGGCAGGGTTTTTGTTTATTTTAAGTGGAAGTAGGAGAACTAAAATAAATAAAAG
AGTGAAACTGAGGTGGTTTT

Downstream 100 bases:

>100_bases
AGTCCCGCATTGTGCGGGACTTTAATTTAAATAATCAGCAGGGTAACTACCCCGAACCATTAACCTCAACCAAAGCCACT
CTTAGATAGTTTGTTGAGAT

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MLKEITDSIKEIENWIIDKRRQLHKYPEKSACEVKTKEVLKSTLEDLGIEVVEGYYTTGLTAVIRGKLSGSKDKTIGLRF
DMDALEMEEKTELDFKSQNPGLMHACGHDGHMAMGLGCAVVLNKFRDKFAGNIKLIFQPAEEDALNGGGARYMIEDGVLH
DEPGVDAMVGVHIWPTLNVGTAGTRVGPIMAASDPFKIRVKGKGVHASLPHMGTDPILIASQIVTNLQSIVSRNIDPFEQ
AVVSTGTIQGGTAHNTIPDEVEIMGTVRTFDDNIRQVVKEKMQEIVTKTAESLGGQGELEYTFGYPPTVNNEKMVCVAQK
AIKAVLGDENYIPVQRPAPGGEDFAYFAREVPSAFIWLGYNQENEQIFPPHNPYYNFNEGILIWGTEIYCNIALEWLRNN

Sequences:

>Translated_400_residues
MLKEITDSIKEIENWIIDKRRQLHKYPEKSACEVKTKEVLKSTLEDLGIEVVEGYYTTGLTAVIRGKLSGSKDKTIGLRF
DMDALEMEEKTELDFKSQNPGLMHACGHDGHMAMGLGCAVVLNKFRDKFAGNIKLIFQPAEEDALNGGGARYMIEDGVLH
DEPGVDAMVGVHIWPTLNVGTAGTRVGPIMAASDPFKIRVKGKGVHASLPHMGTDPILIASQIVTNLQSIVSRNIDPFEQ
AVVSTGTIQGGTAHNTIPDEVEIMGTVRTFDDNIRQVVKEKMQEIVTKTAESLGGQGELEYTFGYPPTVNNEKMVCVAQK
AIKAVLGDENYIPVQRPAPGGEDFAYFAREVPSAFIWLGYNQENEQIFPPHNPYYNFNEGILIWGTEIYCNIALEWLRNN
>Mature_400_residues
MLKEITDSIKEIENWIIDKRRQLHKYPEKSACEVKTKEVLKSTLEDLGIEVVEGYYTTGLTAVIRGKLSGSKDKTIGLRF
DMDALEMEEKTELDFKSQNPGLMHACGHDGHMAMGLGCAVVLNKFRDKFAGNIKLIFQPAEEDALNGGGARYMIEDGVLH
DEPGVDAMVGVHIWPTLNVGTAGTRVGPIMAASDPFKIRVKGKGVHASLPHMGTDPILIASQIVTNLQSIVSRNIDPFEQ
AVVSTGTIQGGTAHNTIPDEVEIMGTVRTFDDNIRQVVKEKMQEIVTKTAESLGGQGELEYTFGYPPTVNNEKMVCVAQK
AIKAVLGDENYIPVQRPAPGGEDFAYFAREVPSAFIWLGYNQENEQIFPPHNPYYNFNEGILIWGTEIYCNIALEWLRNN

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=451, Percent_Identity=25.2771618625277, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 44274; Mature: 44274

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEITDSIKEIENWIIDKRRQLHKYPEKSACEVKTKEVLKSTLEDLGIEVVEGYYTTGL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCC
TAVIRGKLSGSKDKTIGLRFDMDALEMEEKTELDFKSQNPGLMHACGHDGHMAMGLGCAV
EEEEEECCCCCCCCEEEEEECCHHHHCCHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
VLNKFRDKFAGNIKLIFQPAEEDALNGGGARYMIEDGVLHDEPGVDAMVGVHIWPTLNVG
HHHHHHHHHCCCEEEEEECCHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEEEECCC
TAGTRVGPIMAASDPFKIRVKGKGVHASLPHMGTDPILIASQIVTNLQSIVSRNIDPFEQ
CCCCCCCCEEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AVVSTGTIQGGTAHNTIPDEVEIMGTVRTFDDNIRQVVKEKMQEIVTKTAESLGGQGELE
HHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
YTFGYPPTVNNEKMVCVAQKAIKAVLGDENYIPVQRPAPGGEDFAYFAREVPSAFIWLGY
EEECCCCCCCCCEEEEHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHCCCEEEEEEE
NQENEQIFPPHNPYYNFNEGILIWGTEIYCNIALEWLRNN
CCCCCEECCCCCCCCCCCCCEEEEECEEEEEEEHHHHHCC
>Mature Secondary Structure
MLKEITDSIKEIENWIIDKRRQLHKYPEKSACEVKTKEVLKSTLEDLGIEVVEGYYTTGL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCC
TAVIRGKLSGSKDKTIGLRFDMDALEMEEKTELDFKSQNPGLMHACGHDGHMAMGLGCAV
EEEEEECCCCCCCCEEEEEECCHHHHCCHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
VLNKFRDKFAGNIKLIFQPAEEDALNGGGARYMIEDGVLHDEPGVDAMVGVHIWPTLNVG
HHHHHHHHHCCCEEEEEECCHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEEEECCC
TAGTRVGPIMAASDPFKIRVKGKGVHASLPHMGTDPILIASQIVTNLQSIVSRNIDPFEQ
CCCCCCCCEEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AVVSTGTIQGGTAHNTIPDEVEIMGTVRTFDDNIRQVVKEKMQEIVTKTAESLGGQGELE
HHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
YTFGYPPTVNNEKMVCVAQKAIKAVLGDENYIPVQRPAPGGEDFAYFAREVPSAFIWLGY
EEECCCCCCCCCEEEEHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHCCCEEEEEEE
NQENEQIFPPHNPYYNFNEGILIWGTEIYCNIALEWLRNN
CCCCCEECCCCCCCCCCCCCEEEEECEEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]