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The map label for this gene is yaaI [H]

Identifier: 188585244

GI number: 188585244

Start: 632029

End: 632631

Strand: Direct

Name: yaaI [H]

Synonym: Nther_0606

Alternate gene names: 188585244

Gene position: 632029-632631 (Clockwise)

Preceding gene: 188585243

Following gene: 188585245

Centisome position: 19.97

GC content: 35.99

Gene sequence:

>603_bases
ATGGGTTCTCCAAGACACATTAATATCGAAGACTGTGCCTTGTTGATCGTCAACATGCAAAATGATTTTTTAGATAAAAT
CACTTCTTTTGAATGTCCTCGGTGCAGGGAGATTGTTCCTAATTTACAAAAGTTCAAGCATGATATGAAATTTTTTAGAG
TTCCAGTTATCTATACAAAGGAACTGCATAGACCAGGAATGGTTGATTATGGGAAAGAGCTTAGTAAAGAAGGAGAAGAG
CACTGTATAGAAGGAACAAAAGGGGCGGAGATAGTTTCAGAATTGACCCCTGATGAAGATGATCATGTGATTTTAAAACG
TCGCCATAGTGGTTTCTATGCTACAGATCTGGAAATTTTACTAAGAGGCCTTAAGAAAAATACTATTATTTTGGCTGGAG
TTCCTACTAATATTTGTTATTATGCTACTGCATTGGATGCTCATCAATTGAATTTTAATATAATTGCAGTTCCAGATTGT
ACTGCTCCTTCAAAAGGTGTAGATAAAGAACCTTTCCTCAGGAGTATAGAAAAAGTAGTGGGGGAAGTCATGTCTTCCCA
AGAAATTAAAGAGAGATTTCACATGATGGTAGAAGAACCTTAA

Upstream 100 bases:

>100_bases
GTGAATTTAATCTTTTGAATTAAGGAGAAAAGAGTGATGTAATCATTTAACTTTTAAAACAATATAATGTTGATGAAATA
AACTTTAAGGAGGAATGTTT

Downstream 100 bases:

>100_bases
TGATTCTAAGGGCTGAAGCTTGCTGATGTTAGCACAGTTTCAGCCCTTTTTTTTATTAAATTCGACGCTGTTATAAAATT
AAAATTTTACTAGTTATCAA

Product: isochorismatase hydrolase

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MGSPRHINIEDCALLIVNMQNDFLDKITSFECPRCREIVPNLQKFKHDMKFFRVPVIYTKELHRPGMVDYGKELSKEGEE
HCIEGTKGAEIVSELTPDEDDHVILKRRHSGFYATDLEILLRGLKKNTIILAGVPTNICYYATALDAHQLNFNIIAVPDC
TAPSKGVDKEPFLRSIEKVVGEVMSSQEIKERFHMMVEEP

Sequences:

>Translated_200_residues
MGSPRHINIEDCALLIVNMQNDFLDKITSFECPRCREIVPNLQKFKHDMKFFRVPVIYTKELHRPGMVDYGKELSKEGEE
HCIEGTKGAEIVSELTPDEDDHVILKRRHSGFYATDLEILLRGLKKNTIILAGVPTNICYYATALDAHQLNFNIIAVPDC
TAPSKGVDKEPFLRSIEKVVGEVMSSQEIKERFHMMVEEP
>Mature_199_residues
GSPRHINIEDCALLIVNMQNDFLDKITSFECPRCREIVPNLQKFKHDMKFFRVPVIYTKELHRPGMVDYGKELSKEGEEH
CIEGTKGAEIVSELTPDEDDHVILKRRHSGFYATDLEILLRGLKKNTIILAGVPTNICYYATALDAHQLNFNIIAVPDCT
APSKGVDKEPFLRSIEKVVGEVMSSQEIKERFHMMVEEP

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=177, Percent_Identity=28.2485875706215, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 22818; Mature: 22686

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSPRHINIEDCALLIVNMQNDFLDKITSFECPRCREIVPNLQKFKHDMKFFRVPVIYTK
CCCCCCCCHHHEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEH
ELHRPGMVDYGKELSKEGEEHCIEGTKGAEIVSELTPDEDDHVILKRRHSGFYATDLEIL
HCCCCCCCHHHHHHHHCHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCEEHHHHHH
LRGLKKNTIILAGVPTNICYYATALDAHQLNFNIIAVPDCTAPSKGVDKEPFLRSIEKVV
HHHCCCCEEEEEECCCCCEEHHHCCCCEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
GEVMSSQEIKERFHMMVEEP
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GSPRHINIEDCALLIVNMQNDFLDKITSFECPRCREIVPNLQKFKHDMKFFRVPVIYTK
CCCCCCCHHHEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEH
ELHRPGMVDYGKELSKEGEEHCIEGTKGAEIVSELTPDEDDHVILKRRHSGFYATDLEIL
HCCCCCCCHHHHHHHHCHHHHHHCCCCHHHHHHHCCCCCCCCEEEEECCCCCEEHHHHHH
LRGLKKNTIILAGVPTNICYYATALDAHQLNFNIIAVPDCTAPSKGVDKEPFLRSIEKVV
HHHCCCCEEEEEECCCCCEEHHHCCCCEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
GEVMSSQEIKERFHMMVEEP
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]