Click here to switch to the map view.

The map label for this gene is ltaA [H]

Identifier: 188585183

GI number: 188585183

Start: 565922

End: 566947

Strand: Direct

Name: ltaA [H]

Synonym: Nther_0544

Alternate gene names: 188585183

Gene position: 565922-566947 (Clockwise)

Preceding gene: 188585182

Following gene: 188585185

Centisome position: 17.88

GC content: 38.21

Gene sequence:

>1026_bases
ATGTCTTTTATAGATTTGCGTAGTGATACCGTTACCCAACCAACTGAGAAGATGCGTACAGCAATGAGTGAAGCCGCTGT
TGGAGATGATGTATACGAAGAAGATCCAACTATTTTGGAATTGGAAGAGAAAAGTGCTCGAATTTTTAATAAAGAAAAAG
GCTTATTTTTTCCAACAGGAACTATGGCTAATCAAGCAGCAATTCTAGCTCATACTAATCCCGGTGAGGAAATTATAATA
GAAAAGGACATGCATATTTATTTATATGAGGTTGGGGGCTTGGCTTTTTTATCAGGAGTTCAATCGCGACAACTTCCAGG
AAATTTAGGACAACTTGATCCTCAGGAAGTAGAGGCAGCAATCAGACCATCCAATATACATTTTCCGAGGACTTCACTTA
TTTGTTTAGAAAACACTCATAATGTTCATGGAGGTACTGTTATTTCTAAAAGGGACATAGACAAAATTGCAGAAGTGGCT
CATCAATATAAACTGCCTTTGCACTTGGACGGTGCCCGTATTTTTAATGCAGCTACTTATTTACAGACAACTGTGGCAGA
GCTTACTTCGTCATGTGATACTCTCATGTTTTGCCTGTCTAAAGGTTTATCAGCACCAGCGGGATCGATGTTAGTTGGCG
ATGAAAACTTTATCAATAGGGCTAGAAAATGTCGTAAATTACTAGGAGGAGGTATGAGACAAGCCGGGATATTAGCTGCT
GCTGGAATTGTTGCTTTAGAGGAAATGACTAACAGGCTTCAGGAAGACCATGAAAATGCTCTTTTATTAGCCAAAAATTT
AGCAGAAATACCCTGGATCGAAATAGATGTAGATAGAGTTGAAACGAATATCATTAGATTCAATTTAGATACATCTCACT
TTAGTGCTGTAGAGTTTGAAAACAAATTAAGAGAAGCTAATATAAAGGTCAATGTCAGCGGTGATGGTGGTGTCAGGATG
GTGACTCATAAAGATGTCACAAGAGACCAAGTAGAGCATGTGGTTTCAGTATTGAAAAGTTTATAG

Upstream 100 bases:

>100_bases
CAGGGTTAAGATCCCCCTTGCTGGTAATAATCGCCAGCAAGGGTTTTTTATCTATCAAATTATTTAAAAAATTTATTAGA
CGGATAAAGGGGAGTCAGAT

Downstream 100 bases:

>100_bases
TTTGAACTAGTTTTTGCTACTTTTATCTGCCTGATAATCAACTTGAAAAACCAGTTTTCCTCTGAGGGCAATTCTATTTC
TGATAGTTTTGGCATTAATT

Product: L-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSFIDLRSDTVTQPTEKMRTAMSEAAVGDDVYEEDPTILELEEKSARIFNKEKGLFFPTGTMANQAAILAHTNPGEEIII
EKDMHIYLYEVGGLAFLSGVQSRQLPGNLGQLDPQEVEAAIRPSNIHFPRTSLICLENTHNVHGGTVISKRDIDKIAEVA
HQYKLPLHLDGARIFNAATYLQTTVAELTSSCDTLMFCLSKGLSAPAGSMLVGDENFINRARKCRKLLGGGMRQAGILAA
AGIVALEEMTNRLQEDHENALLLAKNLAEIPWIEIDVDRVETNIIRFNLDTSHFSAVEFENKLREANIKVNVSGDGGVRM
VTHKDVTRDQVEHVVSVLKSL

Sequences:

>Translated_341_residues
MSFIDLRSDTVTQPTEKMRTAMSEAAVGDDVYEEDPTILELEEKSARIFNKEKGLFFPTGTMANQAAILAHTNPGEEIII
EKDMHIYLYEVGGLAFLSGVQSRQLPGNLGQLDPQEVEAAIRPSNIHFPRTSLICLENTHNVHGGTVISKRDIDKIAEVA
HQYKLPLHLDGARIFNAATYLQTTVAELTSSCDTLMFCLSKGLSAPAGSMLVGDENFINRARKCRKLLGGGMRQAGILAA
AGIVALEEMTNRLQEDHENALLLAKNLAEIPWIEIDVDRVETNIIRFNLDTSHFSAVEFENKLREANIKVNVSGDGGVRM
VTHKDVTRDQVEHVVSVLKSL
>Mature_340_residues
SFIDLRSDTVTQPTEKMRTAMSEAAVGDDVYEEDPTILELEEKSARIFNKEKGLFFPTGTMANQAAILAHTNPGEEIIIE
KDMHIYLYEVGGLAFLSGVQSRQLPGNLGQLDPQEVEAAIRPSNIHFPRTSLICLENTHNVHGGTVISKRDIDKIAEVAH
QYKLPLHLDGARIFNAATYLQTTVAELTSSCDTLMFCLSKGLSAPAGSMLVGDENFINRARKCRKLLGGGMRQAGILAAA
GIVALEEMTNRLQEDHENALLLAKNLAEIPWIEIDVDRVETNIIRFNLDTSHFSAVEFENKLREANIKVNVSGDGGVRMV
THKDVTRDQVEHVVSVLKSL

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=340, Percent_Identity=44.1176470588235, Blast_Score=272, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI193206522, Length=263, Percent_Identity=47.5285171102662, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI193206524, Length=263, Percent_Identity=47.5285171102662, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI193206520, Length=263, Percent_Identity=47.5285171102662, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6320789, Length=335, Percent_Identity=36.4179104477612, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21355439, Length=367, Percent_Identity=42.7792915531335, Blast_Score=267, Evalue=9e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 37622; Mature: 37491

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIDLRSDTVTQPTEKMRTAMSEAAVGDDVYEEDPTILELEEKSARIFNKEKGLFFPTG
CCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCEEEECCHHHHHHHCCCCEEEECC
TMANQAAILAHTNPGEEIIIEKDMHIYLYEVGGLAFLSGVQSRQLPGNLGQLDPQEVEAA
CCCCCEEEEEECCCCCEEEEECCCEEEEEECCCHHHHHCCHHCCCCCCCCCCCHHHHHHH
IRPSNIHFPRTSLICLENTHNVHGGTVISKRDIDKIAEVAHQYKLPLHLDGARIFNAATY
CCCCCCCCCCEEEEEEECCCCCCCCEEECHHHHHHHHHHHHHHCCCEEECCHHHHHHHHH
LQTTVAELTSSCDTLMFCLSKGLSAPAGSMLVGDENFINRARKCRKLLGGGMRQAGILAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHHHH
AGIVALEEMTNRLQEDHENALLLAKNLAEIPWIEIDVDRVETNIIRFNLDTSHFSAVEFE
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCEEEEEHHHEEEEEEEEEECCCHHHHHHHH
NKLREANIKVNVSGDGGVRMVTHKDVTRDQVEHVVSVLKSL
HHHHHCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFIDLRSDTVTQPTEKMRTAMSEAAVGDDVYEEDPTILELEEKSARIFNKEKGLFFPTG
CEEECCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCEEEECCHHHHHHHCCCCEEEECC
TMANQAAILAHTNPGEEIIIEKDMHIYLYEVGGLAFLSGVQSRQLPGNLGQLDPQEVEAA
CCCCCEEEEEECCCCCEEEEECCCEEEEEECCCHHHHHCCHHCCCCCCCCCCCHHHHHHH
IRPSNIHFPRTSLICLENTHNVHGGTVISKRDIDKIAEVAHQYKLPLHLDGARIFNAATY
CCCCCCCCCCEEEEEEECCCCCCCCEEECHHHHHHHHHHHHHHCCCEEECCHHHHHHHHH
LQTTVAELTSSCDTLMFCLSKGLSAPAGSMLVGDENFINRARKCRKLLGGGMRQAGILAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHHHH
AGIVALEEMTNRLQEDHENALLLAKNLAEIPWIEIDVDRVETNIIRFNLDTSHFSAVEFE
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCEEEEEHHHEEEEEEEEEECCCHHHHHHHH
NKLREANIKVNVSGDGGVRMVTHKDVTRDQVEHVVSVLKSL
HHHHHCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.24 {DL-erythro-phenylserine}} 0.12 {DL-threo-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]