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The map label for this gene is mrdA [H]

Identifier: 188585157

GI number: 188585157

Start: 540479

End: 542356

Strand: Direct

Name: mrdA [H]

Synonym: Nther_0518

Alternate gene names: 188585157

Gene position: 540479-542356 (Clockwise)

Preceding gene: 188585156

Following gene: 188585158

Centisome position: 17.07

GC content: 41.16

Gene sequence:

>1878_bases
ATGCTAATGTCTGAAGAGAAAGATAAAGTTATTTTTAAAAGAATACAGGCTTTTTTTGTAATTACAATTTTTTTGTGTTT
CATACTGGTAGCGCGCCTTGCCTATCTACAGGTATTAGAAGGGGATTATTACGAACAAGTTTCAGAAGAAAACCGCATGC
GTAGGATAACTATTGACCCACCGAGAGGAGAAATTTATGGTCGGGATGGCGAATTGTTGGCTAAAAACACCCCTGGGTAT
ACGGTATCTCTTATGGATGTAGGTGGAGCTGACGAAGAAAAGGTGGTCTATTTTTTAAGTGATTACCTGGAAATTGAAGA
ACAAGAAATAAAGGATAAGATTGATTCGCAACGATTTCGTCAATTTGAACCTATACGAATAAAATCAGGATTGAGTTATG
AACAGGTAGCACACTTGGCAGAAAAAAGGACGGAATTACCAGGAGTTATTCTCGAAATACAGCCAGATAGAATCTATACA
CAGGACAAATTATTAGGTCATGTGTTAGGTTATACCGGTGAAATCACCAGGACACAGTTAGATAGAGGCATGGGGGAAGA
AGGTTATAGGCCAGGTGATATTGTAGGCCAATCGGGGCTAGAAAAAACATACCAAGAAGAGCTGCGGGGAGAACCTGGAT
TTCGCCAGGTAGAAGTTAATAGATTCGGTCGAGTGATCAATAATATGGGTGAAGAAGAACCAATTCCAGGTAATAACCTG
CACCTAACCATCGATGTTGAACTTCAAGAATTTGTGTCGGAATCCTTGGACCATGCCATTGAAACCATCCGCGAAGAAGA
AGGGGAAGAATATGAAGATCACCTAGGAGGGGCCTCTGCTGTTGTTATGAATCCTGATAATGGTGAAATACTTTCGATGG
TCAGCTTACCTAAGTATGATCCCAATACATTATACGAGAATTATGCTGATTTGATAGAAGATAGCACACGGCCACTCAGA
AATAGAGCTATTTCTGAAACCTATCCACCAGGCTCAACCTACAAGATGGTAACAGCCTTAGCTGCCCTTGAAGAAAATGA
GCTGGCAGCCGAAGAAACAATTTTAGATGAAGGTAGATACTGGAATCCGCCAAGACCGAGGAACTTTCAAAATCGTGCCC
TTGGTAGAATAGACGTAGTTGATGCCTTGAAATATTCTTCTAACGTGTTCTTTTCTGAAATGGGACATCGCTTAGGTATC
AATCTACTATCCGAATGGAGTAGGAATTTTGGATTTGGTGCGACTACTGGTCTTGAGGATTTAGATGGTGAACATACCGG
GATAGTAGCCGGGGTAGAATATAAAGAGCGACATTTTCAAGAAGCAGAACAGCAGGTTTGGTATCCGGGAGAAACCATCA
TAGCTGCTATGGGCCAGGGTTATCATACTTATACTCCTTTACAATTAGCTAATTATGCTGCTATGTTAGCAAATGAAGGA
ATCCACTACCGGCCTCATCTAGTTAGTGAAGTGACTGACTCTCAAGGTGAAATTGTAGAAAAAACCGGCACTGAAACTCT
TAATGAGTTGGATATCTCTCAAGGAAATTGGGATTTGGTAAAAGAAGGGATGCGACAAGTGGCCCAACCTGGAGGCACTG
CTGGCTGGATGTTTGATGATCTAGAATTTGATGTCGGATTAAAGACAGGGACAGCGGAAGTGGCAGGAAGGCCGTCTCAT
GGTTGGATAGTAGGATTTGCTCCTTATGAAGATCCGGAAATAGCTTTTAGTATTTTTATTGAACACGGTGGCGGTTCAAG
TAGAGTTACTCCTGTTGCAAGGGCTTTAATTGATTACTATTTTGACTTAATGGAGGAAGATGAAGAAGAACCTGAAGAAT
TAGATATGGATACTGACTCAGGCTATGTTGATGAATAA

Upstream 100 bases:

>100_bases
ATATTACTGCAAAGCCTAATTGGTATAATTTTGTATTCATATATTAAACCTTTATTTAAAAAAGGCGGCTACTTATTTAA
GTATTAGACAGAGGTGGATG

Downstream 100 bases:

>100_bases
TTCTGAAAAATAAAATTAAGGGGCTTGTAGGCCCCTTTTTTTATTAGCTTGGCAGGAAAATTTTAAAGATAAAAAGAATA
ATTACCTAATAGATAAATAT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MLMSEEKDKVIFKRIQAFFVITIFLCFILVARLAYLQVLEGDYYEQVSEENRMRRITIDPPRGEIYGRDGELLAKNTPGY
TVSLMDVGGADEEKVVYFLSDYLEIEEQEIKDKIDSQRFRQFEPIRIKSGLSYEQVAHLAEKRTELPGVILEIQPDRIYT
QDKLLGHVLGYTGEITRTQLDRGMGEEGYRPGDIVGQSGLEKTYQEELRGEPGFRQVEVNRFGRVINNMGEEEPIPGNNL
HLTIDVELQEFVSESLDHAIETIREEEGEEYEDHLGGASAVVMNPDNGEILSMVSLPKYDPNTLYENYADLIEDSTRPLR
NRAISETYPPGSTYKMVTALAALEENELAAEETILDEGRYWNPPRPRNFQNRALGRIDVVDALKYSSNVFFSEMGHRLGI
NLLSEWSRNFGFGATTGLEDLDGEHTGIVAGVEYKERHFQEAEQQVWYPGETIIAAMGQGYHTYTPLQLANYAAMLANEG
IHYRPHLVSEVTDSQGEIVEKTGTETLNELDISQGNWDLVKEGMRQVAQPGGTAGWMFDDLEFDVGLKTGTAEVAGRPSH
GWIVGFAPYEDPEIAFSIFIEHGGGSSRVTPVARALIDYYFDLMEEDEEEPEELDMDTDSGYVDE

Sequences:

>Translated_625_residues
MLMSEEKDKVIFKRIQAFFVITIFLCFILVARLAYLQVLEGDYYEQVSEENRMRRITIDPPRGEIYGRDGELLAKNTPGY
TVSLMDVGGADEEKVVYFLSDYLEIEEQEIKDKIDSQRFRQFEPIRIKSGLSYEQVAHLAEKRTELPGVILEIQPDRIYT
QDKLLGHVLGYTGEITRTQLDRGMGEEGYRPGDIVGQSGLEKTYQEELRGEPGFRQVEVNRFGRVINNMGEEEPIPGNNL
HLTIDVELQEFVSESLDHAIETIREEEGEEYEDHLGGASAVVMNPDNGEILSMVSLPKYDPNTLYENYADLIEDSTRPLR
NRAISETYPPGSTYKMVTALAALEENELAAEETILDEGRYWNPPRPRNFQNRALGRIDVVDALKYSSNVFFSEMGHRLGI
NLLSEWSRNFGFGATTGLEDLDGEHTGIVAGVEYKERHFQEAEQQVWYPGETIIAAMGQGYHTYTPLQLANYAAMLANEG
IHYRPHLVSEVTDSQGEIVEKTGTETLNELDISQGNWDLVKEGMRQVAQPGGTAGWMFDDLEFDVGLKTGTAEVAGRPSH
GWIVGFAPYEDPEIAFSIFIEHGGGSSRVTPVARALIDYYFDLMEEDEEEPEELDMDTDSGYVDE
>Mature_625_residues
MLMSEEKDKVIFKRIQAFFVITIFLCFILVARLAYLQVLEGDYYEQVSEENRMRRITIDPPRGEIYGRDGELLAKNTPGY
TVSLMDVGGADEEKVVYFLSDYLEIEEQEIKDKIDSQRFRQFEPIRIKSGLSYEQVAHLAEKRTELPGVILEIQPDRIYT
QDKLLGHVLGYTGEITRTQLDRGMGEEGYRPGDIVGQSGLEKTYQEELRGEPGFRQVEVNRFGRVINNMGEEEPIPGNNL
HLTIDVELQEFVSESLDHAIETIREEEGEEYEDHLGGASAVVMNPDNGEILSMVSLPKYDPNTLYENYADLIEDSTRPLR
NRAISETYPPGSTYKMVTALAALEENELAAEETILDEGRYWNPPRPRNFQNRALGRIDVVDALKYSSNVFFSEMGHRLGI
NLLSEWSRNFGFGATTGLEDLDGEHTGIVAGVEYKERHFQEAEQQVWYPGETIIAAMGQGYHTYTPLQLANYAAMLANEG
IHYRPHLVSEVTDSQGEIVEKTGTETLNELDISQGNWDLVKEGMRQVAQPGGTAGWMFDDLEFDVGLKTGTAEVAGRPSH
GWIVGFAPYEDPEIAFSIFIEHGGGSSRVTPVARALIDYYFDLMEEDEEEPEELDMDTDSGYVDE

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=636, Percent_Identity=31.6037735849057, Blast_Score=301, Evalue=8e-83,
Organism=Escherichia coli, GI1786272, Length=576, Percent_Identity=26.0416666666667, Blast_Score=148, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70672; Mature: 70672

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMSEEKDKVIFKRIQAFFVITIFLCFILVARLAYLQVLEGDYYEQVSEENRMRRITIDP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECC
PRGEIYGRDGELLAKNTPGYTVSLMDVGGADEEKVVYFLSDYLEIEEQEIKDKIDSQRFR
CCCCEECCCCCEEEECCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
QFEPIRIKSGLSYEQVAHLAEKRTELPGVILEIQPDRIYTQDKLLGHVLGYTGEITRTQL
CCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHH
DRGMGEEGYRPGDIVGQSGLEKTYQEELRGEPGFRQVEVNRFGRVINNMGEEEPIPGNNL
HCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCE
HLTIDVELQEFVSESLDHAIETIREEEGEEYEDHLGGASAVVMNPDNGEILSMVSLPKYD
EEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCCCEEEEEECCCCC
PNTLYENYADLIEDSTRPLRNRAISETYPPGSTYKMVTALAALEENELAAEETILDEGRY
CHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WNPPRPRNFQNRALGRIDVVDALKYSSNVFFSEMGHRLGINLLSEWSRNFGFGATTGLED
CCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
LDGEHTGIVAGVEYKERHFQEAEQQVWYPGETIIAAMGQGYHTYTPLQLANYAAMLANEG
CCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
IHYRPHLVSEVTDSQGEIVEKTGTETLNELDISQGNWDLVKEGMRQVAQPGGTAGWMFDD
CCCCCHHHHHHCCCCCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEC
LEFDVGLKTGTAEVAGRPSHGWIVGFAPYEDPEIAFSIFIEHGGGSSRVTPVARALIDYY
CEEECCCCCCCHHHCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
FDLMEEDEEEPEELDMDTDSGYVDE
HHHHHCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLMSEEKDKVIFKRIQAFFVITIFLCFILVARLAYLQVLEGDYYEQVSEENRMRRITIDP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECC
PRGEIYGRDGELLAKNTPGYTVSLMDVGGADEEKVVYFLSDYLEIEEQEIKDKIDSQRFR
CCCCEECCCCCEEEECCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
QFEPIRIKSGLSYEQVAHLAEKRTELPGVILEIQPDRIYTQDKLLGHVLGYTGEITRTQL
CCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHH
DRGMGEEGYRPGDIVGQSGLEKTYQEELRGEPGFRQVEVNRFGRVINNMGEEEPIPGNNL
HCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCE
HLTIDVELQEFVSESLDHAIETIREEEGEEYEDHLGGASAVVMNPDNGEILSMVSLPKYD
EEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCCCCEEEEEECCCCC
PNTLYENYADLIEDSTRPLRNRAISETYPPGSTYKMVTALAALEENELAAEETILDEGRY
CHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WNPPRPRNFQNRALGRIDVVDALKYSSNVFFSEMGHRLGINLLSEWSRNFGFGATTGLED
CCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
LDGEHTGIVAGVEYKERHFQEAEQQVWYPGETIIAAMGQGYHTYTPLQLANYAAMLANEG
CCCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
IHYRPHLVSEVTDSQGEIVEKTGTETLNELDISQGNWDLVKEGMRQVAQPGGTAGWMFDD
CCCCCHHHHHHCCCCCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEC
LEFDVGLKTGTAEVAGRPSHGWIVGFAPYEDPEIAFSIFIEHGGGSSRVTPVARALIDYY
CEEECCCCCCCHHHCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
FDLMEEDEEEPEELDMDTDSGYVDE
HHHHHCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]