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The map label for this gene is gatA

Identifier: 188585103

GI number: 188585103

Start: 490675

End: 492150

Strand: Direct

Name: gatA

Synonym: Nther_0464

Alternate gene names: 188585103

Gene position: 490675-492150 (Clockwise)

Preceding gene: 188585102

Following gene: 188585104

Centisome position: 15.5

GC content: 40.51

Gene sequence:

>1476_bases
ATGAAACTTCATGAACTAACATTAATGGAAGTAAAACGAGGCCTTGAACAAGGGGATTTTAGCTCGGAAGAATTGGTTAC
ATCCGTTTATGACAGAATTGAAGAAACTGAAGACCATATAAAAGCTTATATAACTTTAACTAAGGAACAAGCCTTGGCTC
AATCTAAAGAAATTGATTCTCAAAGGGCTAAGGGTGAAGAATTGGGTCCCCTTGCAGGTATTCCCGTTGCCATCAAAGAC
AATATTTGTACTAAGGATGTGACGACAAGCTGTGCTTCTAAAATTCTGGAAGATTTTGTGCCGCCCTATAATGCCACGGT
TATATCAAAGCTAGCTGAAGCCGGTGCCATTATAGTTGGAAAGACTAATATGGATGAATTCGCCATGGGATCTTCTACTG
AAAACTCGGCCTTTTTTGTAACCAGTAATCCTTGGGATACCGAGAGAGTTCCTGGAGGATCCAGTGGAGGTTCTGCCGCC
AGTGTAGCTTCGGCACAAGTGCCTCTTGCTTTGGGTTCTGATACTGGGGGTTCTATCCGACAACCAGCTTCATTTTGCGG
TGTAGTAGGTATGAAGCCTACATACGGAAGAGTTTCCCGTTATGGATTAGTAGCCTTTGCCTCATCACTAGATCAGATAG
GTCCACTATCCAAAAATGTCGAAGATACCGCGTTAGCTTTAGATATAATTAGTGGCCATGACCATATGGACTCTACTTCA
GTGGATTTAGAGGTTCCCAATCATACAGATTTCCTGAATCAAGATATTGATGAGTTAACTATTGGAATTCCAAAAGAATA
CTACGAATTAGTCGACAACGATGTTAAGAGCTTGGTAGAATCAAGTTTACAATCTATTGAGCCTGAGGCTGGTAATTACA
GACAGGTGTCTTTGCCAACTACAGAACACGCTCTTAGTGCTTATTACTTAATTGCTCCAGCTGAGGCTTCAAGTAATTTA
GCCCGTTTTGATGGTGTTAGATATGGACAACGATTTGCAACAGATGACTTAAAAACCATGTATAATCAAACACGCAAAGA
AGGCTTTGGAAATGAAGTCAAACAAAGAGTGATGTTAGGTACTTATGCTCTTAGCGCAGGTTATTATGATGCCTTGTATT
TAAAAGCATTAAAAGTTCGCAGTTTGATTAAACAAGAATTTGAACAGGTATTTTCTGAATGTGATGTACTGATTGCGCCA
ACTACTCCAACTGTCCCCTTCCGGGAAGGTGAGAATGTGGATGATCCTCTCACTATGTACAAAAACGATATTTGTACAGC
ACCTGTTAATCTAGCAGGACTGCCGTCCATTTCTATACCCTGTGGTTTTAGCAATGGATTGCCTGTAGGATTACAGGTTA
TCGGAAAGGCCTTTGATGAGGGTAAGGTTATTCAAGTTGCTCATAAAATTGAACAAATGTTACAGGTGTTTAAAAACGCA
CCCCAACAGGGATTAGATAATCAAGGGGGGAAATAA

Upstream 100 bases:

>100_bases
ATTCATTAACCAATGATAAAGCTTTGGAAAATGCTCCTGAAACAGAAGGCAATTATTTTAAAGTTCCAAGAATAATTGAA
TAAAGCGGGGGAGTGATAAA

Downstream 100 bases:

>100_bases
CGATGGCAAATTACGAAACTGTTATTGGTCTCGAGGTGCATTCTGAATTATTAACATCAACCAAGATTTTCTGTGGATGT
TCAACTAGCTTTGGTGATGA

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKLHELTLMEVKRGLEQGDFSSEELVTSVYDRIEETEDHIKAYITLTKEQALAQSKEIDSQRAKGEELGPLAGIPVAIKD
NICTKDVTTSCASKILEDFVPPYNATVISKLAEAGAIIVGKTNMDEFAMGSSTENSAFFVTSNPWDTERVPGGSSGGSAA
SVASAQVPLALGSDTGGSIRQPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPLSKNVEDTALALDIISGHDHMDSTS
VDLEVPNHTDFLNQDIDELTIGIPKEYYELVDNDVKSLVESSLQSIEPEAGNYRQVSLPTTEHALSAYYLIAPAEASSNL
ARFDGVRYGQRFATDDLKTMYNQTRKEGFGNEVKQRVMLGTYALSAGYYDALYLKALKVRSLIKQEFEQVFSECDVLIAP
TTPTVPFREGENVDDPLTMYKNDICTAPVNLAGLPSISIPCGFSNGLPVGLQVIGKAFDEGKVIQVAHKIEQMLQVFKNA
PQQGLDNQGGK

Sequences:

>Translated_491_residues
MKLHELTLMEVKRGLEQGDFSSEELVTSVYDRIEETEDHIKAYITLTKEQALAQSKEIDSQRAKGEELGPLAGIPVAIKD
NICTKDVTTSCASKILEDFVPPYNATVISKLAEAGAIIVGKTNMDEFAMGSSTENSAFFVTSNPWDTERVPGGSSGGSAA
SVASAQVPLALGSDTGGSIRQPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPLSKNVEDTALALDIISGHDHMDSTS
VDLEVPNHTDFLNQDIDELTIGIPKEYYELVDNDVKSLVESSLQSIEPEAGNYRQVSLPTTEHALSAYYLIAPAEASSNL
ARFDGVRYGQRFATDDLKTMYNQTRKEGFGNEVKQRVMLGTYALSAGYYDALYLKALKVRSLIKQEFEQVFSECDVLIAP
TTPTVPFREGENVDDPLTMYKNDICTAPVNLAGLPSISIPCGFSNGLPVGLQVIGKAFDEGKVIQVAHKIEQMLQVFKNA
PQQGLDNQGGK
>Mature_491_residues
MKLHELTLMEVKRGLEQGDFSSEELVTSVYDRIEETEDHIKAYITLTKEQALAQSKEIDSQRAKGEELGPLAGIPVAIKD
NICTKDVTTSCASKILEDFVPPYNATVISKLAEAGAIIVGKTNMDEFAMGSSTENSAFFVTSNPWDTERVPGGSSGGSAA
SVASAQVPLALGSDTGGSIRQPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPLSKNVEDTALALDIISGHDHMDSTS
VDLEVPNHTDFLNQDIDELTIGIPKEYYELVDNDVKSLVESSLQSIEPEAGNYRQVSLPTTEHALSAYYLIAPAEASSNL
ARFDGVRYGQRFATDDLKTMYNQTRKEGFGNEVKQRVMLGTYALSAGYYDALYLKALKVRSLIKQEFEQVFSECDVLIAP
TTPTVPFREGENVDDPLTMYKNDICTAPVNLAGLPSISIPCGFSNGLPVGLQVIGKAFDEGKVIQVAHKIEQMLQVFKNA
PQQGLDNQGGK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=41.4829659318637, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI195972892, Length=238, Percent_Identity=28.9915966386555, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI166795287, Length=169, Percent_Identity=33.7278106508876, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=441, Percent_Identity=37.18820861678, Blast_Score=270, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=477, Percent_Identity=27.2536687631027, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17556264, Length=300, Percent_Identity=25.3333333333333, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=158, Percent_Identity=38.6075949367089, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=201, Percent_Identity=32.3383084577114, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=201, Percent_Identity=32.3383084577114, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=234, Percent_Identity=25.6410256410256, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=230, Percent_Identity=26.9565217391304, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=39.1509433962264, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6319685, Length=444, Percent_Identity=30.8558558558559, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320448, Length=527, Percent_Identity=25.0474383301708, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648113, Length=459, Percent_Identity=41.1764705882353, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24652985, Length=490, Percent_Identity=27.5510204081633, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922090, Length=490, Percent_Identity=27.5510204081633, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24652981, Length=490, Percent_Identity=27.5510204081633, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24652983, Length=490, Percent_Identity=27.5510204081633, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24648435, Length=512, Percent_Identity=25.9765625, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648437, Length=512, Percent_Identity=25.9765625, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45550774, Length=512, Percent_Identity=26.3671875, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078093, Length=514, Percent_Identity=26.2645914396887, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=25.6410256410256, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648441, Length=442, Percent_Identity=26.6968325791855, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648439, Length=442, Percent_Identity=26.6968325791855, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356731, Length=499, Percent_Identity=22.4448897795591, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_NATTJ (B2A5W7)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916648.1
- ProteinModelPortal:   B2A5W7
- SMR:   B2A5W7
- GeneID:   6315342
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0464
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53153; Mature: 53153

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 154-154 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHELTLMEVKRGLEQGDFSSEELVTSVYDRIEETEDHIKAYITLTKEQALAQSKEIDS
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHH
QRAKGEELGPLAGIPVAIKDNICTKDVTTSCASKILEDFVPPYNATVISKLAEAGAIIVG
HHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEE
KTNMDEFAMGSSTENSAFFVTSNPWDTERVPGGSSGGSAASVASAQVPLALGSDTGGSIR
CCCCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCCC
QPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPLSKNVEDTALALDIISGHDHMDSTS
CCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCCCE
VDLEVPNHTDFLNQDIDELTIGIPKEYYELVDNDVKSLVESSLQSIEPEAGNYRQVSLPT
EEEECCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
TEHALSAYYLIAPAEASSNLARFDGVRYGQRFATDDLKTMYNQTRKEGFGNEVKQRVMLG
HHHHHEEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TYALSAGYYDALYLKALKVRSLIKQEFEQVFSECDVLIAPTTPTVPFREGENVDDPLTMY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHH
KNDICTAPVNLAGLPSISIPCGFSNGLPVGLQVIGKAFDEGKVIQVAHKIEQMLQVFKNA
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
PQQGLDNQGGK
CCCCCCCCCCC
>Mature Secondary Structure
MKLHELTLMEVKRGLEQGDFSSEELVTSVYDRIEETEDHIKAYITLTKEQALAQSKEIDS
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHH
QRAKGEELGPLAGIPVAIKDNICTKDVTTSCASKILEDFVPPYNATVISKLAEAGAIIVG
HHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEE
KTNMDEFAMGSSTENSAFFVTSNPWDTERVPGGSSGGSAASVASAQVPLALGSDTGGSIR
CCCCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCCC
QPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPLSKNVEDTALALDIISGHDHMDSTS
CCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCCCE
VDLEVPNHTDFLNQDIDELTIGIPKEYYELVDNDVKSLVESSLQSIEPEAGNYRQVSLPT
EEEECCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
TEHALSAYYLIAPAEASSNLARFDGVRYGQRFATDDLKTMYNQTRKEGFGNEVKQRVMLG
HHHHHEEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TYALSAGYYDALYLKALKVRSLIKQEFEQVFSECDVLIAPTTPTVPFREGENVDDPLTMY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHH
KNDICTAPVNLAGLPSISIPCGFSNGLPVGLQVIGKAFDEGKVIQVAHKIEQMLQVFKNA
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
PQQGLDNQGGK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA