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The map label for this gene is frvX [H]

Identifier: 188584954

GI number: 188584954

Start: 329673

End: 330701

Strand: Direct

Name: frvX [H]

Synonym: Nther_0313

Alternate gene names: 188584954

Gene position: 329673-330701 (Clockwise)

Preceding gene: 188584953

Following gene: 188584958

Centisome position: 10.41

GC content: 39.16

Gene sequence:

>1029_bases
ATGGAGCTATTGAAAAAACTCACTCAAACACCTGGAATACCGGGAAGAGAGGAGCCTATTGCGGAATTAATCAAAGAAGA
AATGAACCAAATTTGCGATGAAGTATGGGTAGATCCCTTGGGAAGTGTAATAGGACTTAAAAAAGGTAATGGAAACAAAA
AGGTAATGGTTGCTGGTCACATGGATGAGATAGGTTTTATAGTAAAGCATATCGATAAGAATGGATTTATTCGCTTACAG
CCTGTAGGTGGCTTCGATCCCCGAAGTTTAATGGCTCAAAGGGTGATTGTTCATGGAAAGGAAGACTTGATTGGCAATTT
AGCCCCAGCTACTAAGCCAATTCATGTCTTGAGTCCTGAAGAGAAGAAAAAACAACTTCAAGTAAAGGATTATTTTGTTG
ATTTGGGTCTTTCTGGTGAAAAAGTCAAGGAATTGGTAGAAATCGGTGACCCTGTCACCCTAAAACAAGATTTTGAAGAA
ATCGGGAACATGTATAGTAGTAAATCCCTTGATGACAGAGTGGGAGTATATGTCATGTTAGAAGCTGCAAAACAGCTTAA
GGACCATGATGCAGATATTTATCTTGTAGCTACCTCTCAGGAGGAAGTGGGTATTAGAGGAGCCATGACATCTTCTTATG
GCATCGAGCCTGATGTAGGCATTGCCCTTGATGTGACTATAGCGGCAGATACTCCAGGAAGCGAGGAATCAGAACAGGTT
ACCAAATTAGGTGAAGGTGCAGCTATTAAAATTATGGACTCTGCTAGCATAACTAATAGAAAAGTACTTCAGACATTAAA
AGACCTAGCCAATGAAAAAGATATTAATCATCAAATGGAAATACTACCTAAAGGAGGAACCGATGCTGGTTCAATCCAGA
GAAGCAAATCTGGAATTCCTGTGGGGACAATATCTATACCATGCAGGTATGTACATACAGTCAATGAAATGATCCATAAA
GAGGATTTAGATGCGTCAGTAAACTTACTATCTGCTTTCCTTGCTGAAGCAAATTTTAACGAATTTTAA

Upstream 100 bases:

>100_bases
AAAATAGCTATTGATATTTGGTAGCTTGATAAGGATATTTACTGGATATTTAGGTCATTAATTTTTGAAATATAACTTAT
TTCAGAGGAGGAATGAATTT

Downstream 100 bases:

>100_bases
TCTGGAGATGCTAACGAATTTATCTGGAGATGCTAATTACAACTGAACTTAGCTTTCCATATAAAAGACCCCATCCCTAT
ATTACAGGATTGGGGTCTTT

Product: Cellulase

Products: D-glucose; GDP

Alternate protein names: TET; TET aminopeptidase; Leucyl aminopeptidase; PhTET2 [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MELLKKLTQTPGIPGREEPIAELIKEEMNQICDEVWVDPLGSVIGLKKGNGNKKVMVAGHMDEIGFIVKHIDKNGFIRLQ
PVGGFDPRSLMAQRVIVHGKEDLIGNLAPATKPIHVLSPEEKKKQLQVKDYFVDLGLSGEKVKELVEIGDPVTLKQDFEE
IGNMYSSKSLDDRVGVYVMLEAAKQLKDHDADIYLVATSQEEVGIRGAMTSSYGIEPDVGIALDVTIAADTPGSEESEQV
TKLGEGAAIKIMDSASITNRKVLQTLKDLANEKDINHQMEILPKGGTDAGSIQRSKSGIPVGTISIPCRYVHTVNEMIHK
EDLDASVNLLSAFLAEANFNEF

Sequences:

>Translated_342_residues
MELLKKLTQTPGIPGREEPIAELIKEEMNQICDEVWVDPLGSVIGLKKGNGNKKVMVAGHMDEIGFIVKHIDKNGFIRLQ
PVGGFDPRSLMAQRVIVHGKEDLIGNLAPATKPIHVLSPEEKKKQLQVKDYFVDLGLSGEKVKELVEIGDPVTLKQDFEE
IGNMYSSKSLDDRVGVYVMLEAAKQLKDHDADIYLVATSQEEVGIRGAMTSSYGIEPDVGIALDVTIAADTPGSEESEQV
TKLGEGAAIKIMDSASITNRKVLQTLKDLANEKDINHQMEILPKGGTDAGSIQRSKSGIPVGTISIPCRYVHTVNEMIHK
EDLDASVNLLSAFLAEANFNEF
>Mature_342_residues
MELLKKLTQTPGIPGREEPIAELIKEEMNQICDEVWVDPLGSVIGLKKGNGNKKVMVAGHMDEIGFIVKHIDKNGFIRLQ
PVGGFDPRSLMAQRVIVHGKEDLIGNLAPATKPIHVLSPEEKKKQLQVKDYFVDLGLSGEKVKELVEIGDPVTLKQDFEE
IGNMYSSKSLDDRVGVYVMLEAAKQLKDHDADIYLVATSQEEVGIRGAMTSSYGIEPDVGIALDVTIAADTPGSEESEQV
TKLGEGAAIKIMDSASITNRKVLQTLKDLANEKDINHQMEILPKGGTDAGSIQRSKSGIPVGTISIPCRYVHTVNEMIHK
EDLDASVNLLSAFLAEANFNEF

Specific function: Functions as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. However, can also cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. Has neither carboxypeptidase nor endop

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=342, Percent_Identity=34.2105263157895, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI87082417, Length=356, Percent_Identity=31.1797752808989, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788727, Length=350, Percent_Identity=28.2857142857143, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 37356; Mature: 37356

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLKKLTQTPGIPGREEPIAELIKEEMNQICDEVWVDPLGSVIGLKKGNGNKKVMVAGH
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECC
MDEIGFIVKHIDKNGFIRLQPVGGFDPRSLMAQRVIVHGKEDLIGNLAPATKPIHVLSPE
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEECCH
EKKKQLQVKDYFVDLGLSGEKVKELVEIGDPVTLKQDFEEIGNMYSSKSLDDRVGVYVML
HHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
EAAKQLKDHDADIYLVATSQEEVGIRGAMTSSYGIEPDVGIALDVTIAADTPGSEESEQV
HHHHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHH
TKLGEGAAIKIMDSASITNRKVLQTLKDLANEKDINHQMEILPKGGTDAGSIQRSKSGIP
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHCCCCCC
VGTISIPCRYVHTVNEMIHKEDLDASVNLLSAFLAEANFNEF
EEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MELLKKLTQTPGIPGREEPIAELIKEEMNQICDEVWVDPLGSVIGLKKGNGNKKVMVAGH
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECC
MDEIGFIVKHIDKNGFIRLQPVGGFDPRSLMAQRVIVHGKEDLIGNLAPATKPIHVLSPE
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEECCH
EKKKQLQVKDYFVDLGLSGEKVKELVEIGDPVTLKQDFEEIGNMYSSKSLDDRVGVYVML
HHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
EAAKQLKDHDADIYLVATSQEEVGIRGAMTSSYGIEPDVGIALDVTIAADTPGSEESEQV
HHHHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHH
TKLGEGAAIKIMDSASITNRKVLQTLKDLANEKDINHQMEILPKGGTDAGSIQRSKSGIP
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHCCCCCC
VGTISIPCRYVHTVNEMIHKEDLDASVNLLSAFLAEANFNEF
EEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]