Click here to switch to the map view.

The map label for this gene is tnsA [H]

Identifier: 188584887

GI number: 188584887

Start: 255977

End: 256807

Strand: Direct

Name: tnsA [H]

Synonym: Nther_0245

Alternate gene names: 188584887

Gene position: 255977-256807 (Clockwise)

Preceding gene: 188584886

Following gene: 188584888

Centisome position: 8.09

GC content: 33.57

Gene sequence:

>831_bases
TTGTCAAACCGTGTAAGGTATCCAAATAAAGTTGATACATACCATGAAATACCTCGTAGAGAAAAATATAAACCATTTAT
TGCTATTCGGAACGTCCCCTCCCTTGGGAGATCCTCACGCATTCTTGGCATAAAAACCAATCGACAACATGAATTTCTTT
CCGACCTAGAAAGAAACTACTACCATATTCTTGAATTCTCCGACCATGTAATTGATATTAGGGAGCAGTTTCCTCTACAG
CTAGAAAGAACATTACTTATTGCCGATGAACTTGGTGTAAAGCATCCAATTCACCCTAAAACTAAGGATCCTGTCCATAT
TACATCAGATTTTAATATAACAATTAAAGAAGATAAAAAAGCAAAAGATTTGGTTCGTACTATAAAAAGAAAAGACGATT
TGTTGGATAAAAGAACTATAGAAAAATTTGAAATTGAAAAGAGATATTGGGACGTTCTTAATATTGACTGGGGTATAGTT
ACGGAAAAAGAAATCGACAAAACATTAGCTCTAAATATTGCAGATGTTATGCATTATTACAGTTTTGATAGTTTAGAATT
TTATCAAATTATTGGTGAAGAAGAAGTGAAAAGCCTCATTGTGACTTTTATTCAAAGACTCATTGATACTGATAAAACTG
TTAGAGAGGTGAGTACTGTTTTTGAAAGAGACTTTCAGTTAGGTAAGGGAGTCGGGATAACCTTATTTAAGCATCTTATA
GCTAGGAAATATATAAAAGTAGATCTTTTTGAACCGTTAAATTTGGACAGGCCTCATCTAAGAGTTGACTTAACTAATAA
GAGCAAGGTTTTGGGGGAGGACGTATCATGA

Upstream 100 bases:

>100_bases
AGAAAGTCTGAACACCAAAAAGAAAATTAGAACATTTTAAAAGAAAGATTGTACAGATATCACCTCTTTAGGATATAATA
GTTCTAAAGAGGTGATTTTT

Downstream 100 bases:

>100_bases
TATTTGTTAATGCGGTTTATCAGTTTGTAGATAAGAATGAAAGAATTCGCATTATTTATGTATCTACAGAAGAGGAATTA
TGTGCATATGTTTATATTGA

Product: TnsA endonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSNRVRYPNKVDTYHEIPRREKYKPFIAIRNVPSLGRSSRILGIKTNRQHEFLSDLERNYYHILEFSDHVIDIREQFPLQ
LERTLLIADELGVKHPIHPKTKDPVHITSDFNITIKEDKKAKDLVRTIKRKDDLLDKRTIEKFEIEKRYWDVLNIDWGIV
TEKEIDKTLALNIADVMHYYSFDSLEFYQIIGEEEVKSLIVTFIQRLIDTDKTVREVSTVFERDFQLGKGVGITLFKHLI
ARKYIKVDLFEPLNLDRPHLRVDLTNKSKVLGEDVS

Sequences:

>Translated_276_residues
MSNRVRYPNKVDTYHEIPRREKYKPFIAIRNVPSLGRSSRILGIKTNRQHEFLSDLERNYYHILEFSDHVIDIREQFPLQ
LERTLLIADELGVKHPIHPKTKDPVHITSDFNITIKEDKKAKDLVRTIKRKDDLLDKRTIEKFEIEKRYWDVLNIDWGIV
TEKEIDKTLALNIADVMHYYSFDSLEFYQIIGEEEVKSLIVTFIQRLIDTDKTVREVSTVFERDFQLGKGVGITLFKHLI
ARKYIKVDLFEPLNLDRPHLRVDLTNKSKVLGEDVS
>Mature_275_residues
SNRVRYPNKVDTYHEIPRREKYKPFIAIRNVPSLGRSSRILGIKTNRQHEFLSDLERNYYHILEFSDHVIDIREQFPLQL
ERTLLIADELGVKHPIHPKTKDPVHITSDFNITIKEDKKAKDLVRTIKRKDDLLDKRTIEKFEIEKRYWDVLNIDWGIVT
EKEIDKTLALNIADVMHYYSFDSLEFYQIIGEEEVKSLIVTFIQRLIDTDKTVREVSTVFERDFQLGKGVGITLFKHLIA
RKYIKVDLFEPLNLDRPHLRVDLTNKSKVLGEDVS

Specific function: Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and tnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas tnsABC and tnsD prom

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR011335
- InterPro:   IPR014832
- InterPro:   IPR014833
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08721 TnsA_C; PF08722 TnsA_N [H]

EC number: NA

Molecular weight: Translated: 32675; Mature: 32544

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRVRYPNKVDTYHEIPRREKYKPFIAIRNVPSLGRSSRILGIKTNRQHEFLSDLERNY
CCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHCE
YHILEFSDHVIDIREQFPLQLERTLLIADELGVKHPIHPKTKDPVHITSDFNITIKEDKK
EEEEECCCCEEEHHHHCCCEEHHEEEEEHHCCCCCCCCCCCCCCEEEECCCEEEEECCCH
AKDLVRTIKRKDDLLDKRTIEKFEIEKRYWDVLNIDWGIVTEKEIDKTLALNIADVMHYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHHHHHH
SFDSLEFYQIIGEEEVKSLIVTFIQRLIDTDKTVREVSTVFERDFQLGKGVGITLFKHLI
CCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ARKYIKVDLFEPLNLDRPHLRVDLTNKSKVLGEDVS
HHHHHEEEEECCCCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SNRVRYPNKVDTYHEIPRREKYKPFIAIRNVPSLGRSSRILGIKTNRQHEFLSDLERNY
CCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHCE
YHILEFSDHVIDIREQFPLQLERTLLIADELGVKHPIHPKTKDPVHITSDFNITIKEDKK
EEEEECCCCEEEHHHHCCCEEHHEEEEEHHCCCCCCCCCCCCCCEEEECCCEEEEECCCH
AKDLVRTIKRKDDLLDKRTIEKFEIEKRYWDVLNIDWGIVTEKEIDKTLALNIADVMHYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHHHHHHHHHHH
SFDSLEFYQIIGEEEVKSLIVTFIQRLIDTDKTVREVSTVFERDFQLGKGVGITLFKHLI
CCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ARKYIKVDLFEPLNLDRPHLRVDLTNKSKVLGEDVS
HHHHHEEEEECCCCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 3010949; 8947057; 10704304 [H]