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The map label for this gene is cwlD [H]

Identifier: 188584875

GI number: 188584875

Start: 241113

End: 241916

Strand: Direct

Name: cwlD [H]

Synonym: Nther_0233

Alternate gene names: 188584875

Gene position: 241113-241916 (Clockwise)

Preceding gene: 188584872

Following gene: 188584877

Centisome position: 7.62

GC content: 34.58

Gene sequence:

>804_bases
TTGATTCTTATAATGGATAAGAATAAACTTGTCAAATATGTGAAATATTCAGCTATAATTTGTTTGTTAATATTAACGGC
GATAATTGGATTTAAATTTCTGAACTCCAGAACAGAAGTTCAACATGGTAAGGAAATTAATATATCTACTGATGACTATA
CCGAACCCTTTGAAACTATCGAAAATGTAGAATTACCTTTACAGGGATTAGTGATTGGAGTGGATCCCGGCCATGGAAGC
TATGATCCGGGTTTTTTTACTGAGGGAGTGAGAGAAAGTGATATTGTCCTGGATATTTCATTAAAATTAAGAAGGCTCTT
AGAACAAGGCGGAGCAGAAGTTGTTATGACTAGGGAAACTGATGAATTAATGGTGGAAAATGATAATGGAACCCAAAGAG
AAGAACTTAGAAAAAGGTCTAAATTATTTTTAAACCAGGATGTAGATTTATATGTAAGTATTCATGGTAATACTGTAGCT
AGTCCTATTTGGAGGGGAGCGCAAACTTTTTTCTATCCAGAAGAAACAGAAGAAGCAGAAGAAAACGATAATAAATGTAA
AAATTTTAATTTGGCTATTTGCATTCAAGATGAACTGCAGCGTGTATTAGCCAATACAGACAGACCTGTACGAATAGGTA
ACTATTACATTTTGCGAGAATTATCAAAACCGGGAGTCTTGGTAGAAGTTGGATTCTTATCTAACCCCCACGAGAGGAAA
TTACTCCAGGATGAAGAATACCAGGAGTTGATTGCCTGGTCCATATATCTCGGTATTATTAAATATCATCATACAGATAT
GTAA

Upstream 100 bases:

>100_bases
GTATAAGATAAAAACAACTAATTCACAAGTAAACAGTAATATCAATAAACAGTGATATCTCCTCCGGCTGTACATATATA
TAAGCTAGGGGGACTAAGAT

Downstream 100 bases:

>100_bases
GGGCTGTACCCAACAACTAGGGTACAGCCCAATTTTTAAGTAATATTTTGTTACTCACAATTTTTATCACATCTTAAAAA
TCTTTTAATATATCCTCAAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MILIMDKNKLVKYVKYSAIICLLILTAIIGFKFLNSRTEVQHGKEINISTDDYTEPFETIENVELPLQGLVIGVDPGHGS
YDPGFFTEGVRESDIVLDISLKLRRLLEQGGAEVVMTRETDELMVENDNGTQREELRKRSKLFLNQDVDLYVSIHGNTVA
SPIWRGAQTFFYPEETEEAEENDNKCKNFNLAICIQDELQRVLANTDRPVRIGNYYILRELSKPGVLVEVGFLSNPHERK
LLQDEEYQELIAWSIYLGIIKYHHTDM

Sequences:

>Translated_267_residues
MILIMDKNKLVKYVKYSAIICLLILTAIIGFKFLNSRTEVQHGKEINISTDDYTEPFETIENVELPLQGLVIGVDPGHGS
YDPGFFTEGVRESDIVLDISLKLRRLLEQGGAEVVMTRETDELMVENDNGTQREELRKRSKLFLNQDVDLYVSIHGNTVA
SPIWRGAQTFFYPEETEEAEENDNKCKNFNLAICIQDELQRVLANTDRPVRIGNYYILRELSKPGVLVEVGFLSNPHERK
LLQDEEYQELIAWSIYLGIIKYHHTDM
>Mature_267_residues
MILIMDKNKLVKYVKYSAIICLLILTAIIGFKFLNSRTEVQHGKEINISTDDYTEPFETIENVELPLQGLVIGVDPGHGS
YDPGFFTEGVRESDIVLDISLKLRRLLEQGGAEVVMTRETDELMVENDNGTQREELRKRSKLFLNQDVDLYVSIHGNTVA
SPIWRGAQTFFYPEETEEAEENDNKCKNFNLAICIQDELQRVLANTDRPVRIGNYYILRELSKPGVLVEVGFLSNPHERK
LLQDEEYQELIAWSIYLGIIKYHHTDM

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 30701; Mature: 30701

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIMDKNKLVKYVKYSAIICLLILTAIIGFKFLNSRTEVQHGKEINISTDDYTEPFETI
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCCCCHHHHH
ENVELPLQGLVIGVDPGHGSYDPGFFTEGVRESDIVLDISLKLRRLLEQGGAEVVMTRET
HCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEECC
DELMVENDNGTQREELRKRSKLFLNQDVDLYVSIHGNTVASPIWRGAQTFFYPEETEEAE
CEEEEECCCCCHHHHHHHHHHHEECCCCEEEEEECCCCHHHHHHCCCCEEECCCCHHHHH
ENDNKCKNFNLAICIQDELQRVLANTDRPVRIGNYYILRELSKPGVLVEVGFLSNPHERK
CCCCCCCCCEEEEEEHHHHHHHHHCCCCCEEECCEEEEEECCCCCEEEEECCCCCCHHHH
LLQDEEYQELIAWSIYLGIIKYHHTDM
HCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILIMDKNKLVKYVKYSAIICLLILTAIIGFKFLNSRTEVQHGKEINISTDDYTEPFETI
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCCCCHHHHH
ENVELPLQGLVIGVDPGHGSYDPGFFTEGVRESDIVLDISLKLRRLLEQGGAEVVMTRET
HCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEECC
DELMVENDNGTQREELRKRSKLFLNQDVDLYVSIHGNTVASPIWRGAQTFFYPEETEEAE
CEEEEECCCCCHHHHHHHHHHHEECCCCEEEEEECCCCHHHHHHCCCCEEECCCCHHHHH
ENDNKCKNFNLAICIQDELQRVLANTDRPVRIGNYYILRELSKPGVLVEVGFLSNPHERK
CCCCCCCCCEEEEEEHHHHHHHHHCCCCCEEECCEEEEEECCCCCEEEEECCCCCCHHHH
LLQDEEYQELIAWSIYLGIIKYHHTDM
HCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]