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The map label for this gene is truA

Identifier: 188584870

GI number: 188584870

Start: 237962

End: 238723

Strand: Direct

Name: truA

Synonym: Nther_0228

Alternate gene names: 188584870

Gene position: 237962-238723 (Clockwise)

Preceding gene: 188584869

Following gene: 188584871

Centisome position: 7.52

GC content: 36.35

Gene sequence:

>762_bases
GTGGACAACATTAAGCTTACAATTGCCTATGATGGGTCAAATTATCATGGATATCAATTACAAGAAAATGCAGTTACTGT
TCAAGAAAAGCTAGAAAAGGCCTTAAATACAGTTTATTATCCATATAAAATAACCCCTTACAGTGCTTCGAGAACTGATT
CGGGAGTACATGCAAAAGGACAGGTAGCCAATTTCTTCGCTCCTAAACAGATCCCAATTCACAAAATTCCTTTAGCTTTA
AATACTAACTTACCCAATGACATTGCTATTATTAATGCTGAGGTAGTGGGTTCTAATTTTCGAGCTCGTAGAGATGCTAT
CAAAAAAGAATATCGTTATTATGTTTACCATGCACCTTATATGGACCCTTTTTGGAGAAATTATGCTTTACATTATCGGG
AACAAAATTTGGATTACACAAAACTTCAACAGGCGGCAAATGACTTTATAGGGAAGCATGATTTTTCCCCCTTTAGAGCT
ATTCAAGGATCAAACCCATCGATTGACCCTGTGAAAGAAATTTATGAATTTGATATCGATGTAAATGCCGGGGAAGATGG
TAATTTGATAATTTTTAAAGTAATCGGAAATGCCTTTTTATATAAAATGGTGAGAATACTAGTAGGTACTTTACTACAAA
TTGGTAATGGAAAACTAGAGATCAATGCAGTCAAAAAGGCACTCCAGACGGGAGACCGTTTGTTTGTAGGGCCAACTGCC
AGTCCTCAGGGACTGGTATTACAGCAAATTTGGTATCCTTGA

Upstream 100 bases:

>100_bases
TAAGCAATTACAGACGCAAATTAGAGATTATTTCGCCATAATTATCCTGGTAACTTTAATGGCCTTTATTCTGTATTCAG
GAATTTGATGGGGTGATTCA

Downstream 100 bases:

>100_bases
CATTGTAAGCTGGTTTGTAATAAAATACATGCATGGATTTATGTGTATAAGCCCCGGAAATACATGATTTAAGGATTTGA
GATTTTTATATGAGGAGGGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MDNIKLTIAYDGSNYHGYQLQENAVTVQEKLEKALNTVYYPYKITPYSASRTDSGVHAKGQVANFFAPKQIPIHKIPLAL
NTNLPNDIAIINAEVVGSNFRARRDAIKKEYRYYVYHAPYMDPFWRNYALHYREQNLDYTKLQQAANDFIGKHDFSPFRA
IQGSNPSIDPVKEIYEFDIDVNAGEDGNLIIFKVIGNAFLYKMVRILVGTLLQIGNGKLEINAVKKALQTGDRLFVGPTA
SPQGLVLQQIWYP

Sequences:

>Translated_253_residues
MDNIKLTIAYDGSNYHGYQLQENAVTVQEKLEKALNTVYYPYKITPYSASRTDSGVHAKGQVANFFAPKQIPIHKIPLAL
NTNLPNDIAIINAEVVGSNFRARRDAIKKEYRYYVYHAPYMDPFWRNYALHYREQNLDYTKLQQAANDFIGKHDFSPFRA
IQGSNPSIDPVKEIYEFDIDVNAGEDGNLIIFKVIGNAFLYKMVRILVGTLLQIGNGKLEINAVKKALQTGDRLFVGPTA
SPQGLVLQQIWYP
>Mature_253_residues
MDNIKLTIAYDGSNYHGYQLQENAVTVQEKLEKALNTVYYPYKITPYSASRTDSGVHAKGQVANFFAPKQIPIHKIPLAL
NTNLPNDIAIINAEVVGSNFRARRDAIKKEYRYYVYHAPYMDPFWRNYALHYREQNLDYTKLQQAANDFIGKHDFSPFRA
IQGSNPSIDPVKEIYEFDIDVNAGEDGNLIIFKVIGNAFLYKMVRILVGTLLQIGNGKLEINAVKKALQTGDRLFVGPTA
SPQGLVLQQIWYP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=29.7794117647059, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI199559785, Length=246, Percent_Identity=28.4552845528455, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=35.2, Blast_Score=143, Evalue=9e-36,
Organism=Drosophila melanogaster, GI116007752, Length=299, Percent_Identity=26.4214046822742, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281362146, Length=259, Percent_Identity=25.0965250965251, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648455, Length=259, Percent_Identity=25.0965250965251, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_NATTJ (B2A4Q5)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916415.1
- ProteinModelPortal:   B2A4Q5
- SMR:   B2A4Q5
- GeneID:   6315986
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0228
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28738; Mature: 28738

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNIKLTIAYDGSNYHGYQLQENAVTVQEKLEKALNTVYYPYKITPYSASRTDSGVHAKG
CCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHEECCEEECCCCCCCCCCCCCCCC
QVANFFAPKQIPIHKIPLALNTNLPNDIAIINAEVVGSNFRARRDAIKKEYRYYVYHAPY
CEEECCCCCCCCCEEEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHCEEEEEECCC
MDPFWRNYALHYREQNLDYTKLQQAANDFIGKHDFSPFRAIQGSNPSIDPVKEIYEFDID
CCHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCE
VNAGEDGNLIIFKVIGNAFLYKMVRILVGTLLQIGNGKLEINAVKKALQTGDRLFVGPTA
ECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCEEEECCCC
SPQGLVLQQIWYP
CCCCEEHHHHCCC
>Mature Secondary Structure
MDNIKLTIAYDGSNYHGYQLQENAVTVQEKLEKALNTVYYPYKITPYSASRTDSGVHAKG
CCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHEECCEEECCCCCCCCCCCCCCCC
QVANFFAPKQIPIHKIPLALNTNLPNDIAIINAEVVGSNFRARRDAIKKEYRYYVYHAPY
CEEECCCCCCCCCEEEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHCEEEEEECCC
MDPFWRNYALHYREQNLDYTKLQQAANDFIGKHDFSPFRAIQGSNPSIDPVKEIYEFDID
CCHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHCCCE
VNAGEDGNLIIFKVIGNAFLYKMVRILVGTLLQIGNGKLEINAVKKALQTGDRLFVGPTA
ECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCEEEECCCC
SPQGLVLQQIWYP
CCCCEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA