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The map label for this gene is rpoA

Identifier: 188584865

GI number: 188584865

Start: 234043

End: 234990

Strand: Direct

Name: rpoA

Synonym: Nther_0223

Alternate gene names: 188584865

Gene position: 234043-234990 (Clockwise)

Preceding gene: 188584864

Following gene: 188584866

Centisome position: 7.39

GC content: 37.97

Gene sequence:

>948_bases
TTGATTGAAATAGAAAAACCTCAGATCGAATGTCATGAGATGAATGAAGAGAGCACTTACGGAAAATTTGTGATAGAACC
CTTAGAAAGAGGTTATGGCACAACACTAGGGAATTCATTGAGACGGATTTTATTATCCTCCCTACCTGGAGCTGCCGTTA
CTTCTGTTAAAATAGATGGTGTTCTACATGAATTTTCTTCAATCCCAGGAGTTGTGGAAGATACTACGGAAATTGTGTTA
AATTTGAAAAGACTGGCATTGAAAATTCACTCTGATGAAAATCAGGTGTTAACCTTAGAAAAAGAGGGTAAAGGTCCTGT
GACTGCAGGTGATATTCAAGCCGGTTCCGATGTAGATGTAATTAATCCTGAACTTCATATAGCCACACTTTCTGAAGACA
CTAGACTTTATATGGAAATAACAGCTAAAAATGGAAGAGGATATGTGAGAGCAGAGAGAAACAAAGAACCAGAACAGCCT
ATTGGGTTGATTCCGGTGGATTCTGTGTTCACACCTGTAACTAAAGCCAACTTCTATGTAGATGATACAAGAGTGGGTCA
AGTAACAGACTATGACCGTCTAACCTTAGAAGTTTGGACTGATGGAAGTTTAAAACCTGATGAAGCTGTTAGTATGTCAG
CCAAGATAATGCAGGAACACTTAGACTTGTTTATTAATCTGACAGAAAAAGCTACTGATATGGAAGTTATGGTTGAAAAA
GAAGAGGACAACAAAGAGAAAATCATGGAACTCAGTATAGACGAACTCGATTTATCTGTCAGGTCATATAACTGTCTAAA
ACGAGCGGGCATCAATACTATAGGAGAACTGACACAAAAAACGCCCGAAGATATGATGAAAGTAAGGAATCTGGGTAAAA
AATCACTAGAAGAAGTTAAGAATAAATTGACGGAGCTAGGCCTGTCATTGAAGGACTCTAAAGAGTAA

Upstream 100 bases:

>100_bases
AATAGACGTACCTGTGGAAGAGCATCTAATTGTTGAGCTTTATTCCCGTTAATAAACCCTCAACTGGATAGTTGTAGTTT
CAAACAAAGGAGGGTTAACT

Downstream 100 bases:

>100_bases
AGGAGGGGAGAAGATGTCTTACAAAAAGCTAAAAAGAAATAAAGACCAGAGAAAAGCCCTCTTAAAAAACCTGACAACTG
CTTTTCTGAGGGACGAAAAA

Product: DNA-directed RNA polymerase, alpha subunit

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MIEIEKPQIECHEMNEESTYGKFVIEPLERGYGTTLGNSLRRILLSSLPGAAVTSVKIDGVLHEFSSIPGVVEDTTEIVL
NLKRLALKIHSDENQVLTLEKEGKGPVTAGDIQAGSDVDVINPELHIATLSEDTRLYMEITAKNGRGYVRAERNKEPEQP
IGLIPVDSVFTPVTKANFYVDDTRVGQVTDYDRLTLEVWTDGSLKPDEAVSMSAKIMQEHLDLFINLTEKATDMEVMVEK
EEDNKEKIMELSIDELDLSVRSYNCLKRAGINTIGELTQKTPEDMMKVRNLGKKSLEEVKNKLTELGLSLKDSKE

Sequences:

>Translated_315_residues
MIEIEKPQIECHEMNEESTYGKFVIEPLERGYGTTLGNSLRRILLSSLPGAAVTSVKIDGVLHEFSSIPGVVEDTTEIVL
NLKRLALKIHSDENQVLTLEKEGKGPVTAGDIQAGSDVDVINPELHIATLSEDTRLYMEITAKNGRGYVRAERNKEPEQP
IGLIPVDSVFTPVTKANFYVDDTRVGQVTDYDRLTLEVWTDGSLKPDEAVSMSAKIMQEHLDLFINLTEKATDMEVMVEK
EEDNKEKIMELSIDELDLSVRSYNCLKRAGINTIGELTQKTPEDMMKVRNLGKKSLEEVKNKLTELGLSLKDSKE
>Mature_315_residues
MIEIEKPQIECHEMNEESTYGKFVIEPLERGYGTTLGNSLRRILLSSLPGAAVTSVKIDGVLHEFSSIPGVVEDTTEIVL
NLKRLALKIHSDENQVLTLEKEGKGPVTAGDIQAGSDVDVINPELHIATLSEDTRLYMEITAKNGRGYVRAERNKEPEQP
IGLIPVDSVFTPVTKANFYVDDTRVGQVTDYDRLTLEVWTDGSLKPDEAVSMSAKIMQEHLDLFINLTEKATDMEVMVEK
EEDNKEKIMELSIDELDLSVRSYNCLKRAGINTIGELTQKTPEDMMKVRNLGKKSLEEVKNKLTELGLSLKDSKE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=297, Percent_Identity=47.1380471380471, Blast_Score=256, Evalue=2e-69,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_NATTJ (B2A4Q0)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916410.1
- ProteinModelPortal:   B2A4Q0
- GeneID:   6315970
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0223
- HOGENOM:   HBG430844
- OMA:   EKPIATI
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35210; Mature: 35210

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIEKPQIECHEMNEESTYGKFVIEPLERGYGTTLGNSLRRILLSSLPGAAVTSVKIDG
CCCCCCCCCHHCCCCCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHH
VLHEFSSIPGVVEDTTEIVLNLKRLALKIHSDENQVLTLEKEGKGPVTAGDIQAGSDVDV
HHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEE
INPELHIATLSEDTRLYMEITAKNGRGYVRAERNKEPEQPIGLIPVDSVFTPVTKANFYV
ECCCEEEEEECCCCEEEEEEEECCCCEEEEECCCCCCCCCCCEEECHHHHCCCCCCEEEE
DDTRVGQVTDYDRLTLEVWTDGSLKPDEAVSMSAKIMQEHLDLFINLTEKATDMEVMVEK
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEC
EEDNKEKIMELSIDELDLSVRSYNCLKRAGINTIGELTQKTPEDMMKVRNLGKKSLEEVK
CCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHH
NKLTELGLSLKDSKE
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MIEIEKPQIECHEMNEESTYGKFVIEPLERGYGTTLGNSLRRILLSSLPGAAVTSVKIDG
CCCCCCCCCHHCCCCCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHH
VLHEFSSIPGVVEDTTEIVLNLKRLALKIHSDENQVLTLEKEGKGPVTAGDIQAGSDVDV
HHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCEE
INPELHIATLSEDTRLYMEITAKNGRGYVRAERNKEPEQPIGLIPVDSVFTPVTKANFYV
ECCCEEEEEECCCCEEEEEEEECCCCEEEEECCCCCCCCCCCEEECHHHHCCCCCCEEEE
DDTRVGQVTDYDRLTLEVWTDGSLKPDEAVSMSAKIMQEHLDLFINLTEKATDMEVMVEK
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEEC
EEDNKEKIMELSIDELDLSVRSYNCLKRAGINTIGELTQKTPEDMMKVRNLGKKSLEEVK
CCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHH
NKLTELGLSLKDSKE
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA